Gingival squamous cell carcinoma in 2 lions under managed care.

Neoplasia is one of the main causes of euthanasia in geriatric captive nondomestic felids. However, few studies have examined oral tumors in these animals. We describe here the clinicopathologic features of gingival squamous cell carcinoma (SCC) in 2 lions (Panthera leo) from separate zoologic collections. In both cases, the lions had a history of sialorrhea, bloody oral discharge, and anorexia. Autopsy findings in both lions were similar and were characterized by poorly circumscribed, friable, and bloody gingival masses with grossly apparent invasion of the mandibular bone; a pathologic fracture was observed in 1 case. Histologically, the masses consisted of poorly circumscribed, unencapsulated, densely cellular proliferations of neoplastic epithelial cells arranged in irregular islands, cords, and anastomosing trabeculae with formation of keratin pearls, which, coupled with positive immunohistochemistry for pancytokeratin, were diagnostic for SCC. Although no metastases were found in either animal, both lions were ultimately euthanized because of poor prognosis.

Outbreaks of dermatopathy caused by Tephrosia noctiflora intoxication in Brazilian cattle.

This study aims to update the knowledge concerning the intoxication by Tephrosia noctiflora in Brazilian cattle herds by reporting new cases of intoxication in lactating cows, their calves and bulls and highlight the epidemiology, clinical signs, pathogenesis, gross, and microscopic lesions. The morbidity and mortality of this intoxication in the farms studied was low. Gross lesions in all affected cattle consisted of dermatitis with hyperpigmentation, crusts, ulceration, erythema, and lichenification in the skin of limbs, ventral abdomen, perianal and perineal areas of lactating calves and adult cattle. Microscopically, the main lesion observed consisted of severe dermatitis with parakeratotic hyperkeratosis, papillated proliferation, and diffuse, accentuated lymphoplasmacytic inflammatory infiltrate in the epidermis and dermis. The presence of skin lesions mainly in the limbs and ventral abdomen of cattle implies the pathogenesis of intoxication is related to a primary contact dermatitis, and the occurrence of similar lesions on the skin of nursing calves reinforces this hypothesis. The putative toxins of T. noctiflora have been thought to be rotenoids. Additional work is needed to define better if these compounds are the main toxin responsible for the dermatopathy observed in these herds.

Identification of a Virulent Newcastle Disease Virus Strain Isolated from Pigeons (Columbia livia) in Northeastern Brazil Using Next-Generation Genome Sequencing.

Newcastle disease virus (NDV), also known as avian paramyxoviruses 1 (APMV-1) is among the most important viruses infecting avian species. Given its widespread circulation, there is a high risk for the reintroduction of virulent strains into the domestic poultry industry, making the surveillance of wild and domestic birds a crucial process to appropriately respond to novel outbreaks. In the present study, we investigated an outbreak characterized by the identification of sick pigeons in a large municipality in Northeastern Brazil in 2018. The affected pigeons presented neurological signs, including motor incoordination, torticollis, and lethargy. Moribund birds were collected, and through a detailed histopathological analysis we identified severe lymphoplasmacytic meningoencephalitis with perivascular cuffs and gliosis in the central nervous system, and lymphoplasmacytic inflammation in the liver, kidney, and intestine. A total of five pigeons tested positive for NDV, as assessed by rRT-PCR targeted to the M gene. Laboratory virus isolation on Vero E6 cells confirmed infection, after the recovery of infectious NVD from brain and kidney tissues. We next characterized the isolated NDV/pigeon/PE-Brazil/MP003/2018 by next-generation sequencing (NGS). Phylogenetic analysis grouped the virus with other NDV class II isolates from subgenotype VI.2.1.2, including two previous NDV isolates from Brazil in 2014 and 2019. The diversity of aminoacid residues at the fusion F protein cleavage site was analyzed identifying the motif RRQKR↓F, typical of virulent strains. Our results all highlight the importance of virus surveillance in wild and domestic birds, especially given the risk of zoonotic NDV.

Dermatopathy caused by Tephrosia noctiflora intoxication in cattle.

An outbreak of a disease characterized by emaciation, dermatitis with erythema, alopecia, foul-smelling exudation, crusting, hyperpigmentation, lichenification, and edema of fore- and hindlimbs, chest and dewlap is described affecting cattle in the State of Alagoas, Northeastern Brazil. Microscopically, the main lesions were characterized by diffuse dermatitis with infiltration of lymphocytes, histiocytes, parakeratotic hyperkeratosis and acanthosis. The plant Tephrosia noctiflora, which exhibited signs of consumption, infested the grazing areas of cattle. To test its toxicity, T. noctiflora was harvested, dried in the shade, crushed and sourced at a concentration of 50% mixed with commercial food for three guinea pigs. The main clinical signs in guinea pigs included weight loss and multifocal, moderate to severe areas of alopecia, diffuse erythema of the skin, vaginal edema and hematuria. Microscopically, lymphocytic and histiocytic dermatitis, parakeratotic hyperkeratosis and acanthosis were noted in guinea pigs. This experiment confirms that T. noctiflora is the cause of outbreaks of dermatitis observed in cattle grazing in areas infested by this plant.

What are the characteristics that influence the sitting time in university students? / Quais são as características que influenciam o tempo sentado em universitários?

Abstract The objective was to estimate the relationship between sociodemographic characteristics, link with the university, behavioral, biological, and self-rated related to health, with time sitting in university students at federal institutions in the state of Bahia, Brazil. This cross-sectional study was carried out with university students from six institutions (Federal University "Recôncavo da Bahia", Federal University of "Bahia", Federal University of "Oeste da Bahia", University of International Integration of Afro-Brazilian Lusophony, Federal University of "Vale do São Francisco" and Federal University of "Sul da Bahia") in the state from Bahia in 2019. Time sitting in hours per day was investigated and relation sociodemographic, link with the university, behavioral, biological, self-rated of stress and health variables. Path analysis was used to apply multivariate linear regression. The significance status was 5%. A total of 1,217 university students participated of the study. The final model accounts for 6% of sitting time. Self-rated health as positive (β: -0.117; p: <0.001), increasing age (β: -0.115; p: <0.001) and physical activity practice (β: -0.113; p: <0.001) contributed to the decrease in sitting time. The increase in the amount of inadequate eating habits (β: 0.063; p: 0.032) favored the increase in sitting time. The adjustment indices were satisfactory. Concluded that self-rated health as positive, advancing age and physical activities practice were determinants of reduced sitting time, on the other hand, irregular eating behaviors favored sedentary behavior.

Resumo O objetivo foi estimar a associação entre as características sociodemográficas, de vínculo com a universidade, comportamentais, biológicas e de autoavaliação relacionada a saúde, com o tempo sentado em universitários de instituições federais do estado da Bahia, Brasil. Este estudo transversal foi realizado com universitários de seis instituições (Universidade Federal do Recôncavo da Bahia, Universidade Federal da Bahia, Universidade Federal do Oeste da Bahia, Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Universidade Federal do Vale do São Francisco e Universidade Federal do Sul da Bahia) do estado da Bahia em 2019. Foi investigado o tempo sentado em horas/dia em relação as variáveis sociodemográficas, de vínculo com a universidade, comportamentais, biológicas e de autoavaliação do estresse e saúde. Empregou-se a análise de caminhos por meio da regressão linear múltipla. O nível de significância foi de 5%. Participaram do estudo 1.217 universitários. O modelo final explicou 6% do tempo sentado. Os universitários que autoavaliaram a saúde como positiva (β: -0,117; p: <0,001), aqueles com maior idade (β: -0,115; p: <0,001) e que apresentam mais tempo de prática de atividades físicas (β: -0,113; p: <0,001) apresentaram diminuição do tempo sentado. O aumento da quantidade de hábitos alimentares inadequados (β: 0,063; p: 0,032) favoreceu o aumento do tempo sentado. Os índices de ajuste foram satisfatórios. Concluiu-se que a autoavaliação da saúde como positiva, o avanço da idade e a prática de atividades físicas foram determinantes da redução do tempo sentado, por outro lado, os comportamentos alimentares irregulares favoreceram o sedentarismo.

Encephalopathy caused by Talisia esculenta intoxication in pregnant ewes and their newborn lambs.

An outbreak of acute encephalopathy occurred in pregnant ewes and their newborn lambs associated with consumption of Talisia esculenta fruits and bark. Clinical signs in 5 adult pregnant ewes included drooling, bloat, tachypnea, depression, ataxia, body shaking, difficulty in rising, and recumbency. Three neonatal lambs born to some of those ewes had similar clinical signs. No significant gross abnormalities were observed on autopsy. Histologically, neuronal necrosis, axonal and dendritic swelling, and loss of Purkinje neurons were observed in the cerebellum. The observation of similar neurologic clinical signs and lesions in pregnant ewes and their neonatal lambs suggests that the toxic principle of T. esculenta crosses the placenta and reaches the fetus.

Mudanças percebidas no estilo de vida no distanciamento social: validade preliminar do questionário / Change perceptions of lifestyle of social distance: preliminary validity of questionnaire

Este estudo teve como objetivos: 1) apresentar o questionário "Percepção das Mudanças no Estilo de Vida Durante o Período de Distanciamento Social" (PERMEV ), elaborado para a aplicação em adultos; 2) estimar a validade de face e conteúdo, e a reprodutibilidade. Foram elaboradas 40 questões agrupadas aos domínios: Alimentação, Atividade física, Comportamento sedentário, Sono, Controle do estresse, Relacionamentos, Comportamentos preventivos e Comportamentos durante o distan-ciamento social. Especialistas na área da saúde realizaram o julgamento da adequação e pertinência do conteúdo das questões. Posteriormente, o instrumento foi reestruturado e submetido à análise da validade de clareza por trabalhadores e estudantes de instituições de ensino superior. Em seguida, realizou-se a análise da reprodutibilidade com a aplicação do teste e reteste com intervalo de 15 a 30 dias e utilizou-se o coeficiente Kappa (K) para a concordância dos dados. O nível de significância es-tatística adotado foi de 5%. Participaram 75 especialistas para a etapa de validade de face e conteúdo, com valores médios de adequação de 89,3% e de pertinência de 89,7%. A validade de clareza foi de 90,2%. Participaram da reprodutibilidade 83 estudantes e servidores de instituições de ensino supe-rior brasileiras e o nível de concordância variou de K de 0,182 a 0,584. Conclui-se que o questionário PERMEV apresentou validade de face e conteúdo e de clareza excelente (≥ 90%), bem como a vali-dade de reprodutibilidade foi razoável para avaliar as mudanças no estilo de vida de adultos durante períodos de distanciamento social, a exemplo, a pandemia da COVID-19

The objectives were: 1) to present the questionnaire "Perception of Changes in Lifestyle During Social Dis-tance Period " (PERMEV ), designed for application in adults; 2) to estimate the face and content validity, and reproducibility. Forty questions were created, grouped into the following domains: Food, Physical Activ-ity, Sedentary Behavior, Sleep, Stress Control, Relationships, Preventive Behaviors and Behaviors during the pandemic. Specialists in the health area performed the judgment of the adequacy and relevance of the content of the questions. Subsequently, the instrument was restructured and submitted to clarity validity analysis by workers and students from higher education institutions. Then, the reproducibility analysis was performed with the application of the test and retest with an interval of 15 to 30 days and the Kappa coefficient (K) was used for data agreement. The level of statistical significance adopted was 5%. Total of 75 specialists participated in the face and content validation, and the mean values of adequacy was 89.3% and relevance was 89.7%. Clarity validity was 90.2%. Eighty-three students and workers from Brazilian higher education institutions participated in reproducibility, and the level of agreement ranged from K from 0.182 to 0.584. Concluded that the PERMEV questionnaire had excellent face and content validity and clarity (≥90%), as well as the reproducibility level was reasonable to assess changes in adult lifestyle during social distance period, for example, the COVID-19 pandemic

Construct validity of the Individual Lifestyle Profile scale in times of social distancing / Validade de constructo da escala Perfil do Estilo de Vida Individual em tempos de distanciamento social

The aims of the study were to estimate the level of reliability and factorial validity of the "Individual Lifestyle Profile" scale in times of social distancing, through confirmatory factor analysis (CFA). Therefore, the "Individual Lifestyle Profile" scale in times of social distancing was used in a study carried out with samples of students and employees of higher education institutions from different Brazilian regions. The final sample consisted of 4,694 adults who have answered the online form. For construct evaluation, internal consistency analysis was performed using Cronbach's Alpha (a) and Spearman's correlation. The CFA was used to test the hypothetical factor structure of the scale. Overall internal consistency was a = 0.778 and there were significant correlations, however, less than ± 0.799 for items from the same constructs and ± 0.499 among items from different constructs. In the CFA, after adjustments to the model structure, all indicators were adequate (Goodness-of-fit Index: 0.976; Comparative Fit Index: 0.937; Normalized Fit Indices: 0.932; Tucker-Lewis Indi-ces: 0.914; Root Mean Square Error of Approximation: 0.047; Root Mean-Square Residual: 0.031; Standardized Root Mean-Square Residual: 0.0337), with the exception for chi-square p values and the ratio between chi-square and degrees of freedom. It is concluded that the "Individual Lifestyle Profile" scale in times of social distancing has shown satisfactory internal consistency and factor structure to guide the assessment of lifestyle (individual or groups) and interventions to promote healthy lifestyles

Os objetivos do estudo foram estimar o nível de confiabilidade e validade fatorial da escala "Perfil do Estilo de Vida Individual " em tempos de distanciamento social, por meio da análise fatorial confirmatória (AFC). Para tanto, a escala "Perfil do Estilo de Vida Individual " em tempos de distanciamento social foi empregada em um estudo realizado com amostras de estudantes e servidores de instituições de ensino superior de diferen-tes regiões brasileiras. A amostra final correspondeu a 4.694 adultos que responderam o formulário on-line. Para a avaliação de constructo foi realizada a análise de consistência interna via Alfa de Cronbach's (a) e correlação de Spearman. Empregou-se a AFC para testar a estrutura fatorial hipotética da escala. A con-sistência interna geral foi de a de 0,778 e houve correlações significativas, porém, inferiores a ± 0,799 para os itens dos mesmos constructos e ± 0,499 entre os itens de constructos diferentes. Na AFC, após ajustes na estrutura do modelo, ocorreu a adequação para todos os indicadores (Goodness-of-fit Index: 0,976; Compa-rative Fit Index: 0,937; Normalized Fit Indices: 0,932; Tucker-Lewis Indices: 0,914; Root Mean Square Error of Approximation: 0,047; Root Mean-Square Residual: 0,031; Standardized Root Mean-Square Residual: 0,0337), com a exceção para os valores de p do Qui-quadrado e razão entre Qui-quadrado e graus de liberdade. Conclui-se que a escala "Perfil do Estilo de Vida Individual " em tempos de distanciamento social, mostrou consistência interna e estrutura fatorial satisfatórias para orientar a avaliação do estilo de vida (individual ou de grupos) e as intervenções para promover estilos de vida saudáveis

Validade Preliminar da Escala Perfil do Estilo de Vida Individual para uso online em tempos de distanciamento social / Preliminary validity of Individual Lifestyle Profile Scale for online use in times of social distancing

Este estudo teve como objetivo estimar a validade de conteúdo e o nível de reprodutibilidade da escala "Perfil do Estilo de Vida Individual" em tempos de distanciamento social. Os itens da escala original proposta por Nahas, Barros e Francalacci, foram modificados para avaliar o estilo de vida de adultos, de forma online, durante o período distanciamento social, a exemplo, a pandemia da COVID-19. As 15 questões contemplaram comportamentos recomendados por especialistas em saúde durante a pandemia, mantendo-se os cinco domínios originais propostos na versão inicial: alimentação saudável, atividade física, controle do estresse, relacionamentos e comportamentos pre-ventivos. Foram avaliadas a adequação e pertinência por meio do julgamento de especialistas (n = 38) e, posteriormente, os profissionais e estudantes (n = 71) de Instituições de Ensino Superior (IES) avaliaram a clareza dos itens. A reprodutibilidade foi estimada via coeficiente Kappa. O nível de significância adotado foi de 5%. A validade de conteúdo mostrou valores médios de adequação, pertinência e clareza de 92,4%, 87,1% e 78,7%, respectivamente. Participaram na etapa de reprodu-tibilidade 83 estudantes e profissionais de IES e o nível de concordância variou de 0,358 referente ao item "G" (Procuro ocupar a maior parte do meu dia com atividades interessantes) a 0,626 relativo ao item "N" (Mantenho o distanciamento físico e uso máscara sempre que preciso sair). Conclui-se que a escala "Perfil do Estilo de Vida Individual" em tempos de distanciamento social apresentou satisfatória validade de conteúdo e os níveis de reprodutibilidade podem ser considerados aceitáveis para escalas desta natureza

This study aimed to estimate the content validation and the reproducibility level of the "Individual Lifestyle Profile" scale in times of social distancing. The items on the scale originally proposed by Nahas, Barros and Francalacci, were modified to assess the lifestyle of adults, of online form, during social distancing period, for example the COVID-19 pandemic. The 15 questions covered the behavior considered by health experts during a pandemic, maintaining the original five domains proposed in the initial version: healthy eating, physical activity, stress control, preventive behavior and relationships. Adequacy and pertinence were assessed through the judgment of specialists (n = 38) and, subsequently, professionals and students (n = 71) from Higher Education Institutions (HEIs) assess the clarity of the items. Reproducibility was estimated using the Kappa coefficient. The level of significance adopted was 5%. The average of the validity content of adequacy, relevance and clarity was 92.4%, 87.1% and 78.7%, respectively. 83 students and professionals of HEI participated in the reproducibility stage and the level of agreement ranged from 0.358 for item "G" (I try to occupy most of my day with interesting activities) to 0.626 for item "N " (I keep my physical distance and wear a mask whenever I need to leave). Concluded that "Individual Lifestyle Profile" scale in times of social distancing presented satisfactory content validity and reproducibility levels may be acceptable for scales of this nature

Seroprevalence of Trypanosoma cruzi in pregnant women in Midwest Brazil: an evaluation of congenital transmission.

Chagas disease (CD) is caused by the protozoan Trypanosoma cruzi and it is mainly acquired through the vector route, however, blood transfusion and congenital transmission are implicated in the spread of the illness worldwide. The congenital route can occur at any stage of pregnancy and its frequency varies. In the Federal District, in Brazil, the frequency of T. cruzi infection in pregnant women and their offspring has not been updated. Thus, the aim of this study was to estimate the prevalence of T. cruzi infection in pregnant women and the rate of congenital transmission in the Federal District. A cross-sectional study was conducted to estimate the seroprevalence of T. cruzi from 2014 to 2016 in the population of pregnant women attended by the public health service throughout the Federal District and a descriptive cohort for the evaluation of congenital transmission. During the study, prenatal data of 98,895 women were consulted and pregnant women registered in 2016, presenting with positive T. cruzi serology, were part of the descriptive cohort. The estimated prevalence of T. cruzi infection in the three years was 0.19% and the congenital transmission rate was 1/40 (2.5%). Our results have shown that, although the main routes of transmission of CD have been interrupted, there is still a risk of congenital transmission in the Federal District. This present study highlights the need for the continuous implementation of a screening program for pregnant women and timely treatment of infected newborns and children.

Poisonous plants for ruminants in the dairy region of Pernambuco, Northeastern Brazil / Plantas tóxicas para ruminantes na bacia leiteira de Pernambuco, Nordeste do Brasil

A survey carried out in the dairy region of Pernambuco was conducted to determine the most important poisonous plants for ruminants in the region. A total of 95 farmers from 19 municipalities were interviewed and the grazing areas of ruminants were inspected. According to the survey, the most important poisonous plants for ruminants were Manihot esculenta, Palicourea aeneofusca, Brachiaria decumbens, Indigofera suffruticosa, Ricinus communis and Cestrum axillare. Less reported classes of poisonous plants included nephrotoxic plants and plants that cause abortions or congenic malformations. Psychotria hoffmannseggiana, Psychotria colorata and Psychotria capitata were reported by farmers as the cause of sudden death in cattle. These plants should be better investigated to evaluate their importance as the cause of cattle deaths.(AU)

Neste estudo prospectivo, realizou-se um levantamento sobre a ocorrência das principais plantas tóxicas e a ocorrência de intoxicações por plantas em ruminantes na bacia leiteira de Pernambuco. Para isso, foram entrevistados 95 produtores rurais de 19 municípios e as áreas de pastagem de ruminantes foram inspecionadas. De acordo com o levantamento, as plantas tóxicas mais importantes para ruminantes foram Manihot esculenta, Palicourea aeneofusca, Brachiaria decumbens, Indigofera suffruticosa e Ricinus communis. As classes menos importantes de plantas tóxicas incluíram plantas nefrotóxicas e plantas que causam abortos e malformações congênitas. Psychotria hoffmannseggiana, Psychotria colorata e Psychotria capitata foram relatadas por produtores rurais como a causa de mortes súbitas em bovinos. Essas plantas devem ser investigadas melhor para avaliar sua importância como a causa de mortes de bovinos.(AU)

Equine coital exanthema caused by equid alphaherpesvirus 3: a report of an outbreak in northeastern Brazil / Exantema coital equino causado pelo alphaherpesvirus equídeo tipo 3: relato de um surto no nordeste do Brasil

Equine coital exanthema is a venereal infectious disease poorly reported in horses in Brazil and was never described in the northeastern region of the country. This work aims to describe the clinical and pathological aspects of an outbreak of equine coital exanthema caused by equid alphaherpesvirus 3, occurred in a herd of horses at the semiarid region of the State of Rio Grande do Norte. Main clinical signs consisted of anorexia, hiporexia, fibrinous or purulent secretion in the penis mucosa and vagina. Two mares presented mild to minimal lesions that consisted of scars in the mucosa of the vagina and in the perivulvar region. In a stallion the disease consisted of severe, multifocal, umbilicated-exanthematous ulcers of approximately 1cm in diameter on the penis mucosa. Other areas where ulcers and crusts were focally observed included the skin of the scrotum and on the lips and mucocutaneous junctions of the oral cavity. Histologically, the main lesion consisted of multifocal severe ulcerative and fibrinous necrotizing balanoposthitis and mild multifocal necrotizing, lymphocytic dermatitis in the lips and scrotum. The equide alphaherpesvirus 3 DNA was amplified in blood samples and penis mucosa using the PCR technique. This is the first report of molecular diagnosis of equine coital exanthema affecting horses in northeastern Brazil. Further studies should be carried out in order to investigate the epidemiology and the importance of this herpetic disease in the country.(AU)

O exantema coital equino é uma doença infecciosa venérea pouco relatada em equinos no Brasil e nunca descrita na região Nordeste do país. Este trabalho tem como objetivo descrever os aspectos clínicos e patológicos de um surto de exantema coital equino causado pelo alphaherpesvirus equídeo 3, que ocorreu em um haras na região semiárida do Estado do Rio Grande do Norte. Os principais sinais clínicos consistiram em anorexia, hiporexia, secreção fibrinosa ou purulenta na mucosa do pênis e vagina. Duas éguas apresentavam lesões discretas que consistiam em cicatrizes na mucosa da vagina e na região perivulvar. Em um garanhão, a doença consistia em úlceras umbilicadas-exantematosas severas, multifocais, de aproximadamente 1 cm de diâmetro na mucosa do pênis. Outras áreas onde úlceras e crostas foram observadas focalmente incluíram a pele do escroto, lábios e junções mucocutâneas da cavidade oral. Histologicamente, as principais lesões consistiam em balanopostite multifocal ulcerativa e necrosante fibrinosa grave e dermatite linfocítica necrosante multifocal leve nos lábios e escroto. O DNA do alphaherpesvirus equídeo tipo 3 foi amplificado em amostras de sangue e mucosa do pênis pela técnica de PCR. Este é o primeiro relato de diagnóstico molecular de exantema coital equino afetando cavalos no nordeste do Brasil. Novos estudos devem ser realizados a fim de investigar a epidemiologia e a importância dessa doença herpética no país.(AU)

Poisonous plants for ruminants in the dairy region of Pernambuco, Northeastern Brazil

ABSTRACT: A survey carried out in the dairy region of Pernambuco was conducted to determine the most important poisonous plants for ruminants in the region. A total of 95 farmers from 19 municipalities were interviewed and the grazing areas of ruminants were inspected. According to the survey, the most important poisonous plants for ruminants were Manihot esculenta, Palicourea aeneofusca, Brachiaria decumbens, Indigofera suffruticosa, Ricinus communis and Cestrum axillare. Less reported classes of poisonous plants included nephrotoxic plants and plants that cause abortions or congenic malformations. Psychotria hoffmannseggiana, Psychotria colorata and Psychotria capitata were reported by farmers as the cause of sudden death in cattle. These plants should be better investigated to evaluate their importance as the cause of cattle deaths.

RESUMO: Neste estudo prospectivo, realizou-se um levantamento sobre a ocorrência das principais plantas tóxicas e a ocorrência de intoxicações por plantas em ruminantes na bacia leiteira de Pernambuco. Para isso, foram entrevistados 95 produtores rurais de 19 municípios e as áreas de pastagem de ruminantes foram inspecionadas. De acordo com o levantamento, as plantas tóxicas mais importantes para ruminantes foram Manihot esculenta, Palicourea aeneofusca, Brachiaria decumbens, Indigofera suffruticosa e Ricinus communis. As classes menos importantes de plantas tóxicas incluíram plantas nefrotóxicas e plantas que causam abortos e malformações congênitas. Psychotria hoffmannseggiana, Psychotria colorata e Psychotria capitata foram relatadas por produtores rurais como a causa de mortes súbitas em bovinos. Essas plantas devem ser investigadas melhor para avaliar sua importância como a causa de mortes de bovinos.

Equine coital exanthema caused by equid alphaherpesvirus 3: a report of an outbreak in northeastern Brazil

ABSTRACT: Equine coital exanthema is a venereal infectious disease poorly reported in horses in Brazil and was never described in the northeastern region of the country. This work aims to describe the clinical and pathological aspects of an outbreak of equine coital exanthema caused by equid alphaherpesvirus 3, occurred in a herd of horses at the semiarid region of the State of Rio Grande do Norte. Main clinical signs consisted of anorexia, hiporexia, fibrinous or purulent secretion in the penis mucosa and vagina. Two mares presented mild to minimal lesions that consisted of scars in the mucosa of the vagina and in the perivulvar region. In a stallion the disease consisted of severe, multifocal, umbilicated-exanthematous ulcers of approximately 1cm in diameter on the penis mucosa. Other areas where ulcers and crusts were focally observed included the skin of the scrotum and on the lips and mucocutaneous junctions of the oral cavity. Histologically, the main lesion consisted of multifocal severe ulcerative and fibrinous necrotizing balanoposthitis and mild multifocal necrotizing, lymphocytic dermatitis in the lips and scrotum. The equide alphaherpesvirus 3 DNA was amplified in blood samples and penis mucosa using the PCR technique. This is the first report of molecular diagnosis of equine coital exanthema affecting horses in northeastern Brazil. Further studies should be carried out in order to investigate the epidemiology and the importance of this herpetic disease in the country.

RESUMO: O exantema coital equino é uma doença infecciosa venérea pouco relatada em equinos no Brasil e nunca descrita na região Nordeste do país. Este trabalho tem como objetivo descrever os aspectos clínicos e patológicos de um surto de exantema coital equino causado pelo alphaherpesvirus equídeo 3, que ocorreu em um haras na região semiárida do Estado do Rio Grande do Norte. Os principais sinais clínicos consistiram em anorexia, hiporexia, secreção fibrinosa ou purulenta na mucosa do pênis e vagina. Duas éguas apresentavam lesões discretas que consistiam em cicatrizes na mucosa da vagina e na região perivulvar. Em um garanhão, a doença consistia em úlceras umbilicadas-exantematosas severas, multifocais, de aproximadamente 1 cm de diâmetro na mucosa do pênis. Outras áreas onde úlceras e crostas foram observadas focalmente incluíram a pele do escroto, lábios e junções mucocutâneas da cavidade oral. Histologicamente, as principais lesões consistiam em balanopostite multifocal ulcerativa e necrosante fibrinosa grave e dermatite linfocítica necrosante multifocal leve nos lábios e escroto. O DNA do alphaherpesvirus equídeo tipo 3 foi amplificado em amostras de sangue e mucosa do pênis pela técnica de PCR. Este é o primeiro relato de diagnóstico molecular de exantema coital equino afetando cavalos no nordeste do Brasil. Novos estudos devem ser realizados a fim de investigar a epidemiologia e a importância dessa doença herpética no país.

Intestinal Myxoid Leiomyosarcoma in a Sambar Deer (Rusa unicolor).

A 9-year-old male sambar deer (Rusa unicolor) that died during sedation had a large and firm polypoid mass, which expanded the small intestinal wall and partially obstructed the duodenal lumen. Histopathology revealed a pleomorphic sarcoma composed of stellate to spindloid cells loosely arranged in an abundant myxoid matrix. The cytoplasm of the neoplastic cells was strongly immunopositive for vimentin and smooth muscle actin, but negative for c-KIT, desmin and myoglobin. The findings are consistent with intestinal myxoid leiomyosarcoma, which is rare in cervids and has not been described in the sambar deer, which is an endangered species.

Glässer's disease in swine from Northeastern Brazil / Doença de Glässer em suínos no Nordeste do Brasil

Glässer's disease is an important infectious disorder of swine caused by Haemophilus parasuis. Although well recognized in most regions of Brazil, outbreaks of Glässer's disease have not been described in Northeastern region. For this reason, three municipalities of the Pernambuco State were visited in order to identify histories of high mortality in growing and finishing pigs. The main clinical signs consisted of dry cough, apathy, fever, anorexia, paresis, muscle tremors, motor incoordination, seizures leading to high mortality rates. Nine pigs were necropsied, and fragments of the nervous system, organs of the abdominal and thoracic cavities were collected for histological analysis. In addition, lung and brain fragments were used for DNA extraction and molecular testing by real-time Polymerase Chain Reaction (PCR). Grossly, the main lesions consisted of petechial hemorrhages or ecchymosis on the skin of the face, abdomen, forelimbs, and hind limbs. The main severe lesions consisted of hydropericardium, hemopericardium, fibrinous pericarditis and pleuropneumonia. Microscopically, pericarditis, epicarditis and subepicardial myocarditis, followed by a moderate to severe multifocal pleuropneumonia, fibrinosuppurative and necrotizing were the most frequent lesions observed. Real-time PCR amplified H. parasuis infB gene in all samples analyzed, confirming the presence of this etiologic agent.(AU)

A doença de Glässer é uma importante enfermidade infecciosa de suínos causada pela bactéria Haemophilus parasuis. Embora bem reconhecida na maioria das regiões do Brasil, surtos de doença de Glässer não têm sido descritos na região Nordeste. Por este motivo, três regiões do Estado de Pernambuco foram visitadas com o objetivo de se identificar históricos de alta mortalidade em leitões e suínos em fase de terminação. Nove suínos foram necropsiados e fragmentos do sistema nervoso, órgãos das cavidades abdominal e torácica foram coletados para análise histopatológica. Além disso, fragmentos de pulmão e cérebro foram utilizados para extração de DNA e realização de teste molecular por meio da Reação em Cadeia da Polimerase (PCR) em tempo real. Os principais sinais clínicos consistiram em tosse seca, apatia, febre, anorexia, paresia, tremores musculares, incoordenação motora e convulsões levando a altas taxas de mortalidade. As lesões macroscópicas mais severas consistiam em petéquias e equimoses na pele da face, abdome, membros anteriores e posteriores, além de hidropericárdio, hemopericárdio, pericardite fibrinosa e pleuropneumonia. Microscopicamente, pericardite, epicardite e miocardite subepicárdica, seguidas de pleuropneumonia multifocal moderada a grave, fibrino-supurativa e necrosante foram as lesões mais frequentes observadas. A PCR em tempo real amplificou o gene infB de H. parasuis em todas as amostras analisadas, confirmando a presença deste agente etiológico.(AU)

Hypertrophic osteoarthropathy in a sheep / Osteoartropatia hipertrófica em um ovino

ABSTRACT: This research described the radiological, clinical and pathological aspects of hypertrophic osteoarthropathy in a seven-year-old Santa Inês ram. The main clinical features were lameness of the thoracic and pelvic limbs, rigid, cracking and enlarged of the humeroradial, humeroulnar and tibiofemoral joints, cervical spine stiffness, and severe lordosis of the thoracolumbar vertebrae. Dyspnea, nasal discharge and abdominal breathing were also observed. On radiographic examination of the thoracic and pelvic limbs, osteoarthrosis with the formation of osteophyte bridging, intra-articular calcification and periosteum reaction were observed. At necropsy were reported bilateral osteophytosis in the humeroradial, humeroulnar, tibiofemoral and interphalangeal joints, and fusion of the bodies of thoracic vertebrae T5-13 T6 and T12-T13. Microscopically, extensive areas of fibrosis were observed in the lungs and the affected joints were thickened by well demarcated and perpendicular orientated woven and trabecular bone proliferation in the epiphysis. The periosteum was also expanded by fibrous connective tissue that extended into and between the new proliferated bone. Based on these findings, hypertrophic osteoarthropathy, which is an unusual condition in sheep, was diagnosed.

RESUMO: Este trabalho descreve os aspectos radiográficos e clínico-patológicos de um caso de osteoartropatia hipertrófica em um ovino, macho, de sete anos de idade, da raça Santa Inês. Os principais sinais clínicos consistiram em claudicação dos membros torácicos e pélvicos, rigidez, crepitação e aumento das articulações úmero-radio-ulnar e femoro-tibio-patelar, rigidez na coluna cervical e severa lordose das vértebras toracolombares. Dispneia, secreção nasal e respiração abdominal também foram observadas. No exame radiográfico dos membros torácicos e pélvicos, foram observadas osteoartrose com formação de osteófitos em ponte, calcificação intra-articular e reação do periósteo. À necropsia notou-se osteofitose bilateral nas articulações umerorradioulnar, femorotibiopatelar e interfalangiana e fusão dos corpos das vértebras torácicas T5-T6 e T12-T13. Microscopicamente, havia áreas extensivas de fibrose pulmonar e as articulações afetadas encontravam-se expandidas pela proliferação de tecido ósseo recém-formado e trabecular, bem demarcado na epífise. O periósteo também estava expandido por tecido conjuntivo fibroso que se estendia dentro e entre o novo osso proliferado. Com base nestes achados, osteoartropatia hipertrófica, que é uma condição inusual em ovinos, foi diagnosticada.

Validade de constructo do módulo do questionário ISAQ-A para mensurar barreiras para a prática de atividades físicas no lazer de universitários / Construct validity of module of ISAQ-A questionnaire to measure leisure-time physical activity barriers of undergraduate

O objetivo deste estudo foi analisar a validade de constructo e a consistência interna do módulo do questionário ISAQ-A para mensurar barreiras para a prática de atividades físicas no lazer de universitários, tendo como base a proposta de agrupamento em três fatores. Foi realizado um estudo transversal com amostra de 970 universitários de uma instituição de ensino superior do Estado da Bahia de média etária de 23,5 anos (±5,2 anos) e 54,5% do sexo feminino. O módulo do questionário ISAQ-A mensura 12 tipos de barreiras, com a possibilidade de resposta até três opções, por ordem de importância. Para analisar a validade de constructo, considerou-se a proposta de agrupamento das barreiras em três fatores: barreiras pessoais, barreiras situacionais e barreiras de recursos/oportunidades, mediante a análise fatorial confirmatória (AFC). A consistência interna foi avaliada por meio do alfa de Cronbach's. A consistência interna geral foi de 0,95, e entre os fatores, variou de 0,86 para o fator situacional a 0,90 para o fator de recursos/oportunidades. Os resultados da AFC mostraram adequação da proposta de classificação das barreiras em três fatores (Comparative Fit Index = 0,96; Standardized Root Mean-Square Residual= 0,03; Tucker-Lewis Indices = 0,95; Normalized Fit Indices = 0,96). Conclui-se que o módulo do questionário ISAQ-A para a mensuração de barreiras para a prática de atividades físicas no lazer de universitários apresentou níveis adequados de validade de constructo e de consistência interna.

The objective of this study was to analyze the construct validity and internal consistency of the module of ISAQ-A (Indicadores de Saúde e Qualidade de Vida em Acadêmicos) questionnaire to measure leisure-time physical activity barriers of undergraduate, based on the proposal of grouping in three factors. A cross- sectional study was carried out with a sample of 970 undergraduate from a higher education institution in the state of Bahia with a mean age of 23.5 years (±5.2 years) and 54.5% female. The ISAQ-A questionnaire measures 12 types of barriers, with the possibility of answering up to three options, in order of importance. To analyze the construct validity, we considered the proposal of grouping the barriers into three factors: personal barriers, situational barriers and resources/opportunities barriers, through confirmatory factor analysis (CFA). The internal consistency was assessed using Cronbach's alpha. The overall internal consistency was 0.95 and for the factors ranged from 0.86 for situational factor to 0.90 for resource/opportunity factor. The CFA results showed the adequacy of the proposed classification of barriers in three factors (Comparative Fit Index = 0.96; Standar-dized Root Mean-Square Residual = 0.03; Tucker-Lewis Indices = 0.95; Normalized Fit Indices = 0.96). It was concluded that the module of the ISAQ-A questionnaire for the measurement of leisure-time physical activity barriers of undergraduate, presented adequate levels of construct validity and internal consistency.

Apoio Matricial e a atuação do Profissional de Educação Física do Núcleo de Apoio à Saúde da Família / Matrix Support and the intervention of Professional Physical Education in the Support Nucleus Family ́s Health

O estudo analisou os fatores associados as práticas de Apoio Matricial realizadas pelos Profissionais de Educação Física do Núcleo de Apoio à Saúde da Família (NASF) no Brasil e segundo as suas regiões. Foi realizado um estudo exploratório e descritivo com 296 profissionais de Educação Física credenciados nas equipes do NASF do Brasil. A amostra foi proporcional às regiões do Brasil e modalidades de NASF, sendo realizada entrevista telefônica entre julho a setembro de 2011. Os componentes de Apoio Matricial foram definidos a partir do modelo teórico do trabalho,foram eles: educação em saúde, educação permanente, controle social e clínica ampliada. Os componentes isolados e suas combinações foram associa-das as variáveis pessoais e de condições de trabalho dos profissionais.Para análise dos dados foi utilizado os testes de Regres-são Logística Binária, nas análises brutas e ajustadas,adotando significância de 5%.Os resultados apontaram os profissionais mais jovens(OR = 4,8; IC95%:1,2-18,0), os integrantes da equipe do NASF desde a implantação(OR = 3,9, IC95%: 1,1-14,0) e aqueles que receberam capacitação (OR = 2,6; IC95%:1,3-5,4) realizaram um maior número de atividades simultâneas.Os indicadores relacionados a condições de trabalho estáveis e a capacitação pela gestão de saúde ofereceram maior chance de os profissionais realizarem a prática do apoio matricial. Investimentos na qualificação dos profissionais podem reduzir as divergências na atuação dos profissionais de Educação Física do NASF nas diferentes regiões do Brasil.

The study analyzed the factors associated with the Matrix Support practices performed by the Physical Education Professionals at the Support Center for Family Health (NASF) in Brazil and according to their regions. Was carried out exploratory and descriptive study with 296 professionals of the Physical Education teams accredited of the NASF of Brazil. A sample was proportional regions of Brazil and modalities of NASF and selected by telephone interview, applied in the period from July to September 2011. The indicators of Matrix Support were defined from the theoretical work model: Health Education, Continuing Education, Social Control and Expanded Clinic. The isolated components and their combinations were associated personal and professional working conditions variables. The associations were to estimate through multinomial and binary logistic regression in crude and adjusted analysis, adopting a 5% significance. The results showed that younger professionals (OR: 4.8; CI95%: 1.2-18.0), the members the NASF team since implementation (OR: 3.9; CI%: 1.1-4.0) and those that received capacitation (OR: 2.6; CI%: 1.3-5.4) realized a higher numbers of simultaneous activities. The indicators related the stable working conditions and the training for health management were associated with higher chances of achieving of matrix support. Investment in the training of professionals can reduce the differences in the perfor-mance of professional of Physical Education of NASF in different regions of Brazil.

Prevalence and factors associated with leisure-time physical activity: survey repeated in university students / Prevalência e fatores associados à prática de atividades físicas no lazer: inquérito repetido em estudantes universitários

Abstract The practice of leisure physical activity represents a behavior that must be monitored in different population groups. The objectives of this study were i) to compare the prevalence of leisure-time physical activity in university students along the survey years in three separate years; ii) to analyze the factors associated with practice of leisure-time physical activity in each survey. Three surveys in a college institution in northeastern Brazil in 2010, 2012 and 2014 were carried out. The dependent variable was practice of leisure-time physical activity. Exploratory variables were sociodemographic factors and link with the university. The prevalence was compared using the chi-square test for linear trend and association for Prevalence Ratio. Participation was of 1,084, 1,085 and 1,041 college students in 2010, 2012 and 2014, respectively. The prevalence of leisure-time physical activity was approximately 50% in the three surveys. Women were less active in leisure time on the three surveys. In each survey, associations were different in relation the courses, and Chemistry – teacher training, Computer Science, Pedagogy – teacher training and Linguistics (no specialization) students showed lower levels of leisure-time physical activity and Biomedicine, Geography – Bachelor, Physical Education – teacher training and Geography – teacher training students were more active in leisure time. It was concluded that there was a stabilization of the prevalence of active leisure over time and that women showed lower prevalence of leisure-time physical activity in the three surveys.

Resumo A prática de atividades físicas no lazer representa um comportamento que auxilia na obtenção de potenciais beneficios para a saúde. Os objetivos deste estudo foram i) comparar as prevalências de universitários ativos no lazer entre três inquéritos transvesais; ii) analisar os fatores associados à prática de atividade física no lazer, em cada inquérito. Foram realizados três inquéritos nos anos de 2010, 2012 e 2014. O desfecho deste estudo foram os ativos no lazer (≥1 dia por semana). As variáveis exploratórias foram as sociodemográficas e de vínculo com a universidade. As prevalências entre os inquéritos foram comparadas pelo teste qui-quadrado para tendência e a associação foi estimada pelas Razões de Prevalências. A participação foi de 1.084, 1.085 e 1.041 universitários nos anos de 2010, 2012 e 2014, respectivamente. As prevalências de ativos no lazer foram de aproximadamente 50% nos três inquéritos. As mulheres foram menos ativas no lazer nos três inquéritos. Os universitários vinculados aos cursos da Biomedicina, Geografia – bacharelado, Educação Física – licenciatura e Geografia – licenciatura foram associados com maiores razões de prevalências de prática de atividades físicas no lazer. Os universitários com menores razões de prevalências de prática de atividades físicas no lazer foram provenientes dos cursos de Química – licenciatura, Ciência da computação, Pedagogia e Letras (sem habilitação). Conclui-se que houve a estabilização da prevalência de universitários ativos no lazer ao longo do período de análise e que as mulheres apresentaram menores prevalências de prática de atividades físicas no lazer nos três inquéritos.