Using blood plasma for monitoring organochlorine contaminants in juvenile white sturgeon, Acipenser transmontanus, from the lower Columbia River.

Organochlorine (OC) pesticide concentrations in blood plasma samples from 88 juvenile white sturgeon collected from the lower Columbia River were measured and compared to plasma sex steroid and OC tissue levels previously measured in corresponding fish. Significant squared correlation coefficients between summation operator DDT concentrations in sturgeon plasma and gonads and livers were 0.37 and 0.32, respectively. Significant negative correlations between plasma testosterone concentration and plasma Sigma DDT concentration in male fish (r(2)=0.26), plasma 17beta estradiol concentration and plasma Sigma DDT concentration in female fish (r(2)=0.38) and condition factor and plasma Sigma DDT concentration in all fish were found (r(2)=0.17). These results suggest that blood plasma may be a suitable nondestructive method for monitoring adult sturgeon population for persistent OC contaminants.

Mercury concentrations in gonad, liver, and muscle of white sturgeon Acipenser transmontanus in the lower Columbia River.

This study determined the partitioning of total mercury in liver, gonad, and cheek muscle of white sturgeon (Acipenser transmonatus) in the lower Columbia River. The relationship between tissue mercury concentrations and various physiologic parameters was assessed. White sturgeon were captured in commercial fisheries in the estuary and Bonneville, The Dalles, and John Day Reservoirs. Condition factor (CF), relative weight (Wr), and gonadosomatic index (GSI) were determined for each fish (n = 57). Gonadal tissue was examined histologically to determine sex and stage of maturity. Liver (n = 49), gonad (n = 49), and cheek muscle (n = 57) were analyzed for total mercury using cold-vapor atomic fluorescence spectrophotometry. Tissue protein concentrations were measured by ultraviolet-visible spectroscopy. Plasma was analyzed for testosterone (T), 11-ketotestosterone (KT), and 17ss-estradiol (E2) using radioimmunoassay. Mean tissue mercury concentrations were higher in muscle compared with liver and gonad at all sampling locations, except Bonneville Reservoir where mean liver mercury content was the highest tissue concentration observed in the study. Significant negative correlations between plasma androgens (T and KT) and muscle mercury content and plasma E2 and liver mercury content were found. A significant positive linear relationship between white sturgeon age and liver mercury concentrations was evident. Significant negative correlations between CF and relative weight and gonad and liver mercury content were found. In addition, immature male sturgeon with increased gonad mercury content had decreased GSIs. These results suggest that mercury, in the form of methylmercury, may have an effect on the reproductive potential of white sturgeon.

Plasma androgen correlation, EROD induction, reduced condition factor, and the occurrence of organochlorine pollutants in reproductively immature white sturgeon (Acipenser transmontanus) from the Columbia River, USA.

White sturgeon (Acipenser transmontanus) support an active fishery in the Columbia River, but there is poor reproductive success within the impounded sections. The poor reproductive success has been attributed to hydroelectric development; however, water pollution could be a significant factor. White sturgeon plasma, liver, and gonad samples were collected from four Columbia River locations and a California aquaculture facility. Total length and weight of the fish were measured, and plasma samples were analyzed for testosterone (T), 11-ketotestosterone (KT), 17 beta-estradiol (E2), and vitellogenin. Liver samples were analyzed for chlorinated pesticides and polychlorinated biphenyls, ethoxyresorufin-O-deethylase (EROD) activity and histopathology. Gonads were examined histologically to assess sexual maturity and characterize any lesions. Significant differences by location existed for p,p'-DDE, EROD activity, and condition factor. Plasma T was negatively correlated with p,p'-DDE in males and females, and plasma KT was negatively correlated in males. These data indicate that pollutants could be adversely affecting white sturgeon in the Columbia River basin.

Seasonal changes and tissue distribution of mercury in largemouth bass (Micropterus salmoides) from Dorena Reservoir, Oregon.

Mercury contamination in fish has lead to the issuance of fish consumption advisories in many water bodies of the United States. There is evidence that mercury concentrations may fluctuate seasonally, which could affect consumption advisories. Largemouth bass (Micropterus salmoides) were collected from Dorena Reservoir, Oregon, in spring and summer of 1995 and spring, summer, and fall of 1996. Samples of muscle, liver, and gonad were analyzed for total mercury. There were seasonal differences for mercury in liver and gonads, but not muscle tissue. Liver mercury concentrations were higher in summer and lower in spring whereas ovarian mercury concentrations were higher in fall and lower in spring. This study shows that liver tissue was more sensitive than muscle tissue to seasonal variability of mercury, but these seasonal fluctuations may not influence consumption advisories.

Interaction of 2,2',4,4',5,5'-hexachlorobiphenyl with hepatic cytochrome P-4501A in rainbow trout (Oncorhynchus mykiss).

Di-ortho polychlorinated biphenyls (PCBs) are prominent environmental contaminants and their biological activity in fish may be more significant than previously thought. Four weeks after intraperitoneal (i.p.) injection with 50 or 250 microg 2,2',4,4',5,5'-hexachlorobiphenyl (2HxCB)/g fish, rainbow trout livers were removed and frozen at -80 degrees C or microsomes were prepared. Microsomal ethoxyresorufin O-deethylase (EROD) activity was approximately one and two orders of magnitude greater than controls in fish treated with 50 and 250 microg 2HxCB/g fish, respectively. Cytochrome P4501A (CYP1A) Western immunoblot relative optical density increased with 2HxCB dose. Hepatic CYP1A1 mRNA levels were approximately threefold greater in fish treated with 250 microg 2HxCB/g fish than in controls, while hepatic CYP1A1 mRNA levels in fish treated with 50 microg 2HxCB/g fish were not significantly induced. There was no increase of CYP1A3 mRNA in 2HxCB-treated fish. The study showed 2HxCB induced hepatic EROD activity, CYP1A protein, and CYP1A1 mRNA content in rainbow trout.