RESUMO
One hundred eighty-nine human tumor specimens were tested in a human tumor cloning assay to determine their growth response to human recombinant granulocyte-macrophage colony-stimulating factor. Of these samples 48 were evaluable for response. Growth stimulation to greater than 150% of controls was noted in 1 of 12 lung cancers (8%) and 1 of 14 breast cancers (7%) but in no other instances for an overall rate of 2 of 48 (4.2%). A dose-response effect was not seen with each of the two stimulated samples responding only at the two lowest concentrations tested. In addition, 7 cell lines derived from human tumors were tested using a metabolic CO2 production assay without evidence of growth stimulation. Samples of normal bone marrow displayed the usual dose-dependent stimulation whether grown in agar or assayed metabolically. We conclude that human recombinant granulocyte-macrophage colony-stimulating factor has minimal effect on the growth of the solid tumors tested and that clinical trials to reduce chemotherapy-associated myelo-suppression may proceed without undue concern for enhancement of tumor growth.
Assuntos
Adenocarcinoma/patologia , Neoplasias da Mama/patologia , Carcinoma de Células Escamosas/patologia , Neoplasias do Colo/patologia , Ensaio de Unidades Formadoras de Colônias , Fatores Estimuladores de Colônias/farmacologia , Substâncias de Crescimento/farmacologia , Neoplasias Pulmonares/patologia , Ensaio Tumoral de Célula-Tronco , Fator Estimulador de Colônias de Granulócitos e Macrófagos , Humanos , Proteínas Recombinantes/farmacologia , Células Tumorais CultivadasRESUMO
Four structural polypeptides of Hazara virus, an agent closely related to the Crimean-Congo haemorrhagic fever (C-CHF) viruses, were resolved by SDS-polyacrylamide gel electrophoresis. Three glycoproteins were identified (mol. wt. 84,000, 45,000 and 30,000) and were found to be associated with the virion envelope. A fourth polypeptide (mol. wt. 52,000) was non-glycosylated and associated with the nucleocapsid. The structural proteins of Hazara virus differ markedly from those reported for other bunyaviruses.
Assuntos
Bunyaviridae/análise , Glicoproteínas/análise , Proteínas Virais/análise , Capsídeo/análise , Eletroforese em Gel de Poliacrilamida , Peso Molecular , Proteínas do Envelope ViralRESUMO
The etiologic agent of Korean hemorrhagic fever has been propagated in a human cultured cell line derived from a carcinoma of the lung. The cells, described as type II, alveolar epithelial, support replication of the agent and successive passages. Antigen of the Korean hemorrhagic fever agent is readily detected in infected cells by means of direct or indirect fluorescent antibody techniques. Previous attempts to propagate this agent in vitro had been unsuccessful.
Assuntos
Febre Hemorrágica com Síndrome Renal/microbiologia , Orthohantavírus/crescimento & desenvolvimento , Vírus de RNA/crescimento & desenvolvimento , Antígenos Virais/análise , Linhagem Celular , Orthohantavírus/imunologia , Humanos , Alvéolos Pulmonares/microbiologiaRESUMO
Studies were conducted to define the natural host range of the Korean hemorrhagic fever (KHF) agent in South Korea, and to identify colonized rodents susceptible to this infection. Eight species of field rodents were captured in areas of Korea endemic for KHF and their tissues were examined by immunofluorescence for the presence of KHF antigen. One hundred and fourteen of 817 Apodemus agrarius coreae captured between 1974 and 1978 had one or more positive organs. No positive organ was found in 239 rodents of the other seven species examined. Two hundred and thirty-eight specimens of Apodemus agrarius jejuensis captured on Jeju Island, an area thought to be free of disease, were also negative. Attempted laboratory infection of nine species of rodents captured in the field but maintained in the laboratory was successful only in the two subspecies of Apodemus. The 46 specimens of A. a. jejunesis tested in this manner were all uniformly susceptible to infection as determined by immunofluorescence. Serial sacrifice of experimentally infected A. a. jejuensis revealed viremia of short duration terminating on day 10 postinfection. In contrast, other tissues of this animal, including lung, kidney, liver and parotid gland were positive on day 10 and remained so through the 100-day observation period. When 12 species of colonized laboratory rodents were inoculated with KHF agent five were found to develop KHF antibody by indirect immunofluorescence and two, Calomys callosus and Apodemus agrarious ningpoensis, developed detectable KHF antigen in their tissues.