RESUMO
BACKGROUND/AIMS: Obesity leads to increased risk of thromboembolic events in adults, but few studies have addressed the relationship between obesity and thrombogenic risk during childhood. The aim of this study was to evaluate the prothrombotic state of obese children in comparison with healthy children. METHODS: Thrombin generation, fibrinogen, and D-dimer levels, along with metabolic parameters, were measured in 72 prepubertal children, of which 47 were obese and 25 eutrophic. RESULTS: A significant increase in thrombin generation, fibrinogen, and dyslipidemia was found among obese patients. CONCLUSION: A prothrombotic state develops in childhood obesity during the prepubertal phase.
Assuntos
Doenças Cardiovasculares , Obesidade Infantil , Adulto , Índice de Massa Corporal , Doenças Cardiovasculares/epidemiologia , Doenças Cardiovasculares/etiologia , Criança , Fibrinogênio/análise , Fibrinogênio/metabolismo , Fatores de Risco de Doenças Cardíacas , Humanos , Obesidade Infantil/complicações , Fatores de RiscoRESUMO
OBJECTIVES: To assess the craniofacial skeletal growth in pediatric hematopoietic stem-cell transplantation (HSCT) survivors in comparison with age-sex matched-paired controls. METHODS: A case-controlled retrospective comparison of the craniofacial growth in 25 HSCT children and 25 matched-paired controls was conducted. Craniofacial growth was quantitatively assessed by linear and angular measurements in panoramic radiographic images using ImageJ¯. Stature growth and body weight were obtained through physical examination. Cancer diagnosis, myeloablative conditioning, and HSCT were retrieved from medical records. RESULTS: Patients aged 12.2 years (±3.8; 16 male, 9 female). Radiographic images were obtained on an average of 2.43 (±2.0) years after HSCT. The main malignant diagnosis was acute lymphoblastic leukemia (56%), followed by acute myeloid leukemia (36%) and myelodysplastic syndromes (8%). Total body irradiation was associated with chemotherapy at 80%. Mean age at transplantation was 10 (±4.7) years. HSCT survivors showed reduced a vertical growth of the mandibular ramus (p=0.003). This persisted among individuals below 12 years of age (p=0.017). The HSCT group showed delayed dental eruption, though there was no statistically significant difference (p=0.3668). The HSCT group showed stature deficit, increased weight, and body mass index (Z-score stature: -0.28; Z-score weight: 0.38, respectively). CONCLUSIONS: Pediatric HSCT has decreased vertical craniofacial growth compared to their matched controls. There might be an association between reduced craniofacial vertical growth and reduced estature growth. Further studies to quantitatively investigate the impact of different myeloablative regimens in craniofacial skeletal growth and development.
Assuntos
Transplante de Células-Tronco Hematopoéticas , Leucemia Mieloide Aguda , Criança , Feminino , Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Humanos , Masculino , Estudos Retrospectivos , Condicionamento Pré-Transplante , Irradiação Corporal Total/efeitos adversosRESUMO
OBJECTIVES: To assess the craniofacial skeletal growth in pediatric hematopoietic stem-cell transplantation (HSCT) survivors in comparison with age-sex matched-paired controls. METHODS: A case-controlled retrospective comparison of the craniofacial growth in 25 HSCT children and 25 matched-paired controls was conducted. Craniofacial growth was quantitatively assessed by linear and angular measurements in panoramic radiographic images using ImageJ¯. Stature growth and body weight were obtained through physical examination. Cancer diagnosis, myeloablative conditioning, and HSCT were retrieved from medical records. RESULTS: Patients aged 12.2 years (±3.8; 16 male, 9 female). Radiographic images were obtained on an average of 2.43 (±2.0) years after HSCT. The main malignant diagnosis was acute lymphoblastic leukemia (56%), followed by acute myeloid leukemia (36%) and myelodysplastic syndromes (8%). Total body irradiation was associated with chemotherapy at 80%. Mean age at transplantation was 10 (±4.7) years. HSCT survivors showed reduced a vertical growth of the mandibular ramus (p=0.003). This persisted among individuals below 12 years of age (p=0.017). The HSCT group showed delayed dental eruption, though there was no statistically significant difference (p=0.3668). The HSCT group showed stature deficit, increased weight, and body mass index (Z-score stature: -0.28; Z-score weight: 0.38, respectively). CONCLUSIONS: Pediatric HSCT has decreased vertical craniofacial growth compared to their matched controls. There might be an association between reduced craniofacial vertical growth and reduced estature growth. Further studies to quantitatively investigate the impact of different myeloablative regimens in craniofacial skeletal growth and development.
Assuntos
Humanos , Masculino , Feminino , Criança , Leucemia Mieloide Aguda , Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Estudos Retrospectivos , Irradiação Corporal Total/efeitos adversos , Condicionamento Pré-TransplanteRESUMO
OBJECTIVE: Hematopoietic stem-cell transplantation (HSCT) childhood survivors of hematologic malignancies are prone to develop late osteopenia and osteoporosis. The purpose of this retrospective study was to quantitatively and qualitatively assess bone mineral density (BMD) in HSCT childhood survivors and to compare the effectiveness of both qualitative and quantitative assessment methods. METHODS: DESIGN BMD assessment using panoramic radiographs of childhood HSCT survivors aged 3.69-18.88 years using two radiomorphometric indexes. Case-control double-blinded comparison of panoramic radiographic images from childhood HSCT survivors and age- and sex-matched healthy controls. Quantitative assessment was performed by measuring the cortical bone width bilaterally at the mental foramen level. Qualitative assessment was performed using the mandibular cortical index bilaterally on all panoramic images. RESULTS: Radiographs were taken 6.59-83.95 months after bone marrow transplantation [median±SD=25.92±24.9 months]. Fifty-two panoramic radiographic images were analyzed: 21 from HSCT survivors and 31 from healthy controls aged 3.69-25.1 years [mean±SD=11.89±5.28 years]. The mandibular cortical bone width was 17% smaller in childhood HSCT survivors than in healthy controls (case group: 2.420, control group: 3.307; p=0.00617). Qualitative analysis revealed an increased frequency of severe mandibular cortical erosion in childhood HSCT survivors, although no significant difference was observed (case group: 1.540, control group: 1.490; p=0.32). The interobserver agreement was 85% (Kappa index). CONCLUSIONS: HSCT childhood survivors exhibit quantitative and qualitative mandibular bone impairments. Further studies are needed to establish an association between mandibular cortical bone impairment and osteoporosis.
Assuntos
Densidade Óssea , Transplante de Células-Tronco Hematopoéticas , Mandíbula/diagnóstico por imagem , Radiografia Panorâmica/métodos , Adolescente , Doenças Ósseas Metabólicas , Estudos de Casos e Controles , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Estudos Retrospectivos , Dente/diagnóstico por imagemRESUMO
OBJECTIVE: Hematopoietic stem-cell transplantation (HSCT) childhood survivors of hematologic malignancies are prone to develop late osteopenia and osteoporosis. The purpose of this retrospective study was to quantitatively and qualitatively assess bone mineral density (BMD) in HSCT childhood survivors and to compare the effectiveness of both qualitative and quantitative assessment methods. METHODS: DESIGN BMD assessment using panoramic radiographs of childhood HSCT survivors aged 3.69-18.88 years using two radiomorphometric indexes. Case-control double-blinded comparison of panoramic radiographic images from childhood HSCT survivors and age- and sex-matched healthy controls. Quantitative assessment was performed by measuring the cortical bone width bilaterally at the mental foramen level. Qualitative assessment was performed using the mandibular cortical index bilaterally on all panoramic images. RESULTS: Radiographs were taken 6.59-83.95 months after bone marrow transplantation [median±SD=25.92±24.9 months]. Fifty-two panoramic radiographic images were analyzed: 21 from HSCT survivors and 31 from healthy controls aged 3.69-25.1 years [mean±SD=11.89±5.28 years]. The mandibular cortical bone width was 17% smaller in childhood HSCT survivors than in healthy controls (case group: 2.420, control group: 3.307; p=0.00617). Qualitative analysis revealed an increased frequency of severe mandibular cortical erosion in childhood HSCT survivors, although no significant difference was observed (case group: 1.540, control group: 1.490; p=0.32). The interobserver agreement was 85% (Kappa index). CONCLUSIONS: HSCT childhood survivors exhibit quantitative and qualitative mandibular bone impairments. Further studies are needed to establish an association between mandibular cortical bone impairment and osteoporosis.
Assuntos
Humanos , Masculino , Feminino , Pré-Escolar , Criança , Adolescente , Radiografia Panorâmica/métodos , Densidade Óssea , Transplante de Células-Tronco Hematopoéticas , Mandíbula/diagnóstico por imagem , Dente/diagnóstico por imagem , Doenças Ósseas Metabólicas , Estudos de Casos e Controles , Estudos RetrospectivosRESUMO
The aim of this study was to provide an updated review of dental procedures undertaken at the dental unit of the Onco-hematology service of the Instituto da Criança at the Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo (ICr/HC-FMUSP). We retrospectively reviewed 565 of 1902 medical and dental records of patients diagnosed with onco-hematological diseases who were seen in a 3-year study (January 2015 to December 2017). We assessed data regarding population characteristics, onco-hematological diagnosis and dental procedures performed. Of the selected medical records, preventive dentistry was the most common procedure undertaken in this population, followed by oral maxillofacial surgeries, restorative dentistry and oral mucositis treatment. The most prevalent malignant diagnosis was acute lymphocytic leukemia, and the most prevalent nonmalignant diagnosis was sickle-cell anemia. Preventive dental procedures represent most of the dental procedures undertaken in hospitalized onco-hematological pediatric patients.
Assuntos
Assistência Odontológica para Crianças/estatística & dados numéricos , Neoplasias/terapia , Doenças Estomatognáticas/prevenção & controle , Adolescente , Criança , Pré-Escolar , Prestação Integrada de Cuidados de Saúde , Serviços de Saúde Bucal , Humanos , Lactente , Recém-Nascido , Prontuários Médicos , Estudos Retrospectivos , Doenças Estomatognáticas/terapiaRESUMO
Objective: The purpose of this study was to comparatively evaluate quantitative effects of three different prophylactic methods of surface polishing treatments for primary teeth compared to a standardized control group. Material and Methods: 48 naturally exfoliated primary teeth were selected and randomly assigned into four groups: Control Group teeth receiving only enamel standardization treatment with polishing disc to reduce natural enamel; Group I teeth receiving superficial enamel standardization treatment followed by polishing with a mixture of water and pumice; Group II teeth receiving enamel standardization treatment followed by prophylaxis with paste (Herjos-F, Vigodent S/A Indústria e Comércio, Rio de Janeiro, Brazil); and Group III teeth receiving enamel standardization treatment followed by sodium bicarbonate spray (Profi II Ceramic, Dabi Atlante Indústrias Médico-Odontológicas Ltda, Ribeirão Preto, Brazil). All the procedures were performed by the same operator and the samples were rinsed and stored in distilled water. Comparative assessment of the enamel surface roughness between experimental groups and control group was performed by using a surface profilometer (Mitutoyo SJ400). Results: The results of this study were statistically analysed by using Minitab statistical software (version 17.1.0, 2013). The use of pumice and water led to significantly rougher surfaces than in other groups (i.e. Group I: 1.22 Ra; Group II 0.38 Ra; Group III: 1.01 Ra). Conclusion: Based on this study, one can conclude that use of pumice and water resulted in increased enamel surface roughness in comparison to the surface treatment with bicarbonate spray and prophylaxis paste. (AU)
Objetivos: O objetivo deste estudo foi quantificar e comparar a rugosidade do esmalte dentário após de três diferentes tratamentos profiláticos para polimento de superfície de esmalte em dentes decíduos, em relação a um grupo controle. Material e Métodos: 48 dentes decíduos naturalmente esfoliados foram selecionados e alocados aleatoriamente em quatro grupos. Grupo Controle: recebeu apenas padronização do esmalte através do disco de polimento para redução de dentes naturais. O grupo I recebeu padronização superficial do esmalte seguido de polimento por mistura de água e pedra-pomes; o grupo II recebeu padronização do esmalte seguido da pasta de profilaxia Herjos-F (Vigodent S / A Indústria e Comércio, Rio de Janeiro, Brasil); e o grupo III recebeu padronização da superfície do esmalte seguido de spray de bicarbonato de sódio Profi II Ceramic (Dabi Atlante Indústrias Médico Odontológicas Ltda., Ribeirão Preto, Brasil). Todos os tratamentos de superfície foram realizados pelo mesmo operador, por dez segundos e as amostras foram lavadas e armazenadas em água destilada. A avaliação comparativa da rugosidade superficial do esmalte entre os grupos experimentais e controle foi realizada utilizando-se um perfilômetro de superfície Mitutoyo SJ400. Resultados: Os resultados deste estudo foram analisados estatisticamente com o software estatístico Minitab (versão 17.1.0, 2013). A pedra-pomes e a água geraram significativamente maior aspereza na superfície de esmalte, em comparação aos outros grupos, quando empregado o teste estatístico de Tukey (Grupo I: 1,22 Ra; Grupo II: 0,38 Ra; Grupo III: 1,01 Ra). Conclusão: Com base neste estudo, pode-se concluir que a pedra-pomes e a água resultaram em aumento da rugosidade superficial do esmalte em comparação ao tratamento superficial com spray de bicarbonato e pasta profilática.(AU)
Assuntos
Esmalte Dentário/anormalidades , Profilaxia Dentária , Dente DecíduoRESUMO
The aim of this study was to provide an updated review of dental procedures undertaken at the dental unit of the Onco-hematology service of the Instituto da Criança at the Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo (ICr/HC-FMUSP). We retrospectively reviewed 565 of 1902 medical and dental records of patients diagnosed with onco-hematological diseases who were seen in a 3-year study (January 2015 to December 2017). We assessed data regarding population characteristics, onco-hematological diagnosis and dental procedures performed. Of the selected medical records, preventive dentistry was the most common procedure undertaken in this population, followed by oral maxillofacial surgeries, restorative dentistry and oral mucositis treatment. The most prevalent malignant diagnosis was acute lymphocytic leukemia, and the most prevalent nonmalignant diagnosis was sickle-cell anemia. Preventive dental procedures represent most of the dental procedures undertaken in hospitalized onco-hematological pediatric patients.
Assuntos
Humanos , Recém-Nascido , Lactente , Pré-Escolar , Criança , Adolescente , Doenças Estomatognáticas/prevenção & controle , Assistência Odontológica para Crianças/estatística & dados numéricos , Neoplasias/terapia , Doenças Estomatognáticas/terapia , Prontuários Médicos , Estudos Retrospectivos , Prestação Integrada de Cuidados de Saúde , Serviços de Saúde BucalRESUMO
The aim of this paper is to update the current published content about the craniofacial long-term development disturbances of childhood hematopoietic stem-cell transplantation (HSCT) and the preparative regimens for the onco-hematological malignancies treatments. Methods: Two author sindependently reviewed the published articles about long-term effects of childhood HSCT that fitted into predetermined inclusion/exclusion criteria: clear definition of exposure or intervention, standard outcomes measurement and appropriate statistical analysis. Results: Twelve papers matched all the previous established eligibility criteria and were included in this review. The childrens age at HSCT were related to a higher risk of dental development disturbances, such as agenesis, dental hypoplasia, root stunting, crown-root proportion alterations, and microdontia. Craniofacial vertical growth was impaired in the irradiated patients without antero-posterior or latero-lateral impairment. Temporomandibular joint dysfunction was found to be more prevalent in the patients diagnosed withgraft-versus-host disease. Conclusion: The late effects of craniofacial development disturbances lead to several aesthetic and functional impairment, periodontal bone resorption with consequent impairment of chewing ability, and risk of early tooth loss often associated with lifes quality impairment. Further investigations should be performed to provide accurate information for patients, parent sand health care professionals...
Este artigo visa revisar o conteúdo publicado sobre as alterações tardias em crianças submetidas ao transplante de células-tronco hematopoiéticas(TCTH) e o regime pré-transplante para tratamento das doenças onco-hematológicas. Método: Dois autores independentes conduziram uma revisão simples dos artigos recentemente publicados sobre o tema, utilizando os critérios de exclusão/inclusão pré determinados: definição clara da intervenção clínica realizada, mensuração dos resultados padronizada e análise estatística adequada. Resultados: Doze artigos enquadraram-se em todos os critérios de avaliação pré-determinados para esta revisão. Houve correlação entre a idade da criança no momento do TCTH e maior risco de distúrbios de desenvolvimento dentário, como agenesia, hipoplasia dentária, encurtamento radicular,alterações da proporção coroa-raiz e microdontia.Houve alteração do crescimento craniofacial no sentido vertical sem, no entanto, alterar o crescimento anteroposterior e latero-lateral. Disfunção da articulação temporomandibular foi mais prevalente entre os pacientes com diagnostico de Doença do Enxerto contra o Hospedeiro. Conclusão: Os efeitos tardios do desenvolvimento craniofacial podem levara diversas alterações estéticas e funcionais, reabsorção óssea periodontal, diminuição da capacidade de mastigação e perda dentária precoce com possíveis impactos negativos sobre a qualidade de vida. Mais estudos são necessários para fornecer informações precisas aos pacientes, cuidadores e profissionais de saúde...
Assuntos
Humanos , Criança , Anormalidades Craniofaciais/tratamento farmacológico , Anormalidades Craniofaciais/radioterapia , Dente/crescimento & desenvolvimento , Transplante de Células-Tronco HematopoéticasRESUMO
PURPOSE: To analyze the effects of aqueous ozone irrigation over bone healing in hyperglycemia-induced rats. METHODS: Forty-eight male Wistar rats were allocated into Group H (hyperglycemic) or Group N (control). Monocortical bone wound were performed on femurs' anterolateral face. Wounds were treated with a trans-operatory single irrigation of 100ml of aqueous ozone [0.004mg/ml] whereas control groups received 100ml of pure water (Milli-Q®). Histomorphological and histomorphometrical analyses were accomplished after seven, 14 and 21 days. Kruskal-Wallis and Mann-Whitney statistical tests were applied for bone neoformation quantification and assessment. RESULTS: Aqueous ozone wounds irrigated revealed diffuse hemorrhage and increased neoformed of blood vessels number. There was no statistical significant difference in bone trabeculae neoformation. After seven and 14 days, the number of osteoclasts was higher in aqueous ozone groups than in those treated with pure water. CONCLUSION: Independently of blood glucose levels, aqueous ozone allowed an increase in blood vessels neoformation and osteoclast migration, without affect bone trabeculae neoformation.
Assuntos
Regeneração Óssea/efeitos dos fármacos , Hiperglicemia/fisiopatologia , Ozônio/uso terapêutico , Cicatrização/efeitos dos fármacos , Animais , Glicemia/efeitos dos fármacos , Masculino , Osteoclastos/citologia , Osteoclastos/efeitos dos fármacos , Distribuição Aleatória , Ratos , Ratos Wistar , Valores de Referência , Irrigação Terapêutica/métodos , Fatores de Tempo , Cicatrização/fisiologiaRESUMO
PURPOSE: To analyze the effects of aqueous ozone irrigation over bone healing in hyperglycemia-induced rats. METHODS: Forty-eight male Wistar rats were allocated into Group H (hyperglycemic) or Group N (control). Monocortical bone wound were performed on femurs' anterolateral face. Wounds were treated with a trans-operatory single irrigation of 100ml of aqueous ozone [0.004mg/ml] whereas control groups received 100ml of pure water (Milli-Q®). Histomorphological and histomorphometrical analyses were accomplished after seven, 14 and 21 days. Kruskal-Wallis and Mann-Whitney statistical tests were applied for bone neoformation quantification and assessment. RESULTS: Aqueous ozone wounds irrigated revealed diffuse hemorrhage and increased neoformed of blood vessels number. There was no statistical significant difference in bone trabeculae neoformation. After seven and 14 days, the number of osteoclasts was higher in aqueous ozone groups than in those treated with pure water. CONCLUSION: Independently of blood glucose levels, aqueous ozone allowed an increase in blood vessels neoformation and osteoclast migration, without affect bone trabeculae neoformation.
Assuntos
Animais , Masculino , Ratos , Regeneração Óssea/efeitos dos fármacos , Hiperglicemia/fisiopatologia , Ozônio/uso terapêutico , Cicatrização/efeitos dos fármacos , Glicemia/efeitos dos fármacos , Osteoclastos/citologia , Osteoclastos/efeitos dos fármacos , Distribuição Aleatória , Ratos Wistar , Valores de Referência , Fatores de Tempo , Irrigação Terapêutica/métodos , Cicatrização/fisiologiaRESUMO
A instalação de implantes dentários é um procedimento cirúrgico utilizado para reabilitação funcional e estética em pacientes de diferentes idades, gêneros e condições de saúde. O processo de osseointegração depende primordialmente que a reparação dos tecidos ósseos ocorra na ausência de interferências negativas. As condições sistêmicas observadas nos pacientes diabéticos descompensados causam comprometimento da reparação tecidual, alta susceptibilidade a infecções e complicações microvasculares que podem contraindicar a instalação de implantes metálicos. Entretanto, devido à expansão da prevalência do diabetes mellitus é maior o número de portadores desta doença que necessitam de implantes osseointegrados. A literatura sobre o comprometimento da reparação dos tecidos ósseos observado no diabetes é extensa e está relacionada ao desequilíbrio entre a produção, o metabolismo e o equilíbrio de moléculas reativas de oxigênio. A hipoinsulinemia e a hiperglicemia seriam as principais complicações responsáveis pelos distúrbios no processo reparativo. Objetivando descrever os principais aspectos do diabetes e sua correlação com a reparação óssea e a osseointegração, uma revisão da literatura é apresentada
Dental implants have become the therapy of choice for functional and esthetic rehabilitation in patients of different ages, gender, and health status. Implant osseointegration is primarily dependent on bone tissue repair that should occur in the absence of negative interferences. Systemic conditions observed in the non-controlled diabetic patient cause tissue repair impairment, high susceptibility to infections, and microvascular complications that may contraindicate the installation of metallic implants. However, the ongoing prevalence of diabetes mellitus increases the number of patients with this disease who require dental implants. Literature on bone tissue repair and Diabetes is extensive and related to the imbalance between production, metabolism, and action of reactive oxygen molecules. Hypoinsulinemia and hyperglicemia are the main causes of healing disturbances. Aiming to describe the main aspects of diabetes and their correlation with bone healing and osseointegration, a review of the literature is presented
Assuntos
Diabetes Mellitus , Implantes Dentários , OsseointegraçãoRESUMO
Alguns relatos encontrados na literatura sobre as propriedades da molécula de ozônio de interferir de maneira favorável na reparação de tecidos e da sua ação antimicrobiana têm fundamentado emprego do ozônio com fins terapêuticos no tratamento de diversas doenças. Nenhuma pesquisa ainda conseguiu comprovar, por meio de estudos padronizados, os efeitos terapêuticos sobre os processos de reparação óssea desta molécula triatômica de oxigênio quando diluído em água. Nesta investigação foram avaliados os aspectos histomorfológicos e histomorfométricos do processo de reparo tecidual ósseo após a irrigação com 100mL de 4ppm de ozônio diluído em água Milli-Q® durante a perfuração de feridas monocorticais padronizadas, realizadas por meio de broca trefina (2mm), após 7, 14 e 21 dias, em fêmures de ratos Wistar induzidos e não induzidos ao diabetes por injeção intraperitoneal de Estreptozotocina (STZ- Sigma®). Nos grupos controles as feridas foram irrigadas com de 100mL de água Milli-Q® pura. Os resultados histomorfológicos revelaram que os animais diabéticos que receberam o ozônio apresentaram intensa hemorragia, maior proliferação de vasos sanguíneos e trabeculado ósseo imaturo quando comparados aos animais diabéticos não submetidos à aplicação de ozônio em todos os períodos avaliados. Os animais não diabéticos que receberam ozônio apresentaram intenso infiltrado inflamatório e maior proliferação de vasos sanguíneos quando comparados aos animais do grupo controle. Comparativamente as feridas nos animais que receberam a irrigação por meio de água ozonizada mostraram maior proliferação de vasos sanguíneos e trabéculas ósseas mais imaturas quando comparados aos animais dos grupos que não receberam ozônio.
A avaliação histomorfométrica mostrou as médias percentuais de trabéculas ósseas neoformadas. O estudo estatístico, entre os grupos de animais diabéticos e não diabéticos, não apresentou resultados estatisticamente significantes (7 dias, P=0,362; 14 dias, P=0,54; 21 dias, P=0,351) nos períodos de 7, 14 e 21 dias pós-operatórios. A comparação entre o grupo de animais não diabéticos que receberam e não receberam o ozônio, 21 dias pós-operatórios mostrou resultados estatisticamente significantes (P=0,034) confirmando um retardo no processo de maturação das trabéculas. Os resultados desta pesquisa sugerem que a irrigação de feridas ósseas de animais induzidos e não induzidos ao diabetes empregando solução de 4ppm de ozônio diluídos em água Milli-Q® foram capazes de estimular a proliferação de vasos sanguíneos durante a fase inflamatória da reparação tecidual, sem produzir efeitos tóxicos ou prejudiciais, mas não mostrou benefícios na neoformação de trabéculas ósseas em animais diabéticos.
Some accounts in the medical literature about ozones molecule properties to favorably interfere with tissue repair and its antimicrobial action have based the therapeutic usage of ozone to treat several diseases. No standardized investigation yet succeeded to establish the therapeutic effects over bone repair process of this triatomic molecule of the oxygen when diluted in pure water. In this investigation we evaluated the histomorphological aspects of bone tissue repair process after irrigation with 100 ml of the 4 ppm of the ozone dissolved in Milli-Q ® during drilling of the standardized monocortical wounds performed through trephine drill (2mm), after 7, 14 and 21 days in femurs of rats induced and not induced to diabetes by intraperitoneal injection of streptozotocin (STZ, Sigma®). The wounds of the control groups were irrigated with 100ml of Milli-Q®. The histomorphological evaluation revealed that the diabetic animals that received ozone showed intense hemorrhage, increased proliferation of blood vessels and immature bone trabeculae when compared with diabetic animals not submitted to the application of ozone in all evaluated periods.
The non-diabetic animals that received ozone presented intense inflammatory infiltrate and increased proliferation of blood vessels compared to the control group. Comparatively wounds of the animals that received irrigation through ozonized water showed higher proliferation of blood vessels and bone trabeculae more immature when compared to groups that did not receive ozone. Histomorphometric of bone trabeculae development, comparatively in wounds of diabetic and non diabetic animals showed no statistically significant results (7 days, P=0,362; 14 days, P=0,54; 21 days, P=0,351) . The comparison of bone trabeculae development between wounds in the group of non-diabetic animals that received and did not receive ozone, 21 days postoperatively showed statistically significant (P = 0.034). These results suggests that the irrigation of bone wounds of animals induced and not induced to diabetes with 4 ppm of ozone diluted in Milli-Q® were capable to stimulate the proliferation of blood vessels during the inflammatory phase of tissue repair without producing toxic or detrimental effects but did not improve bone trabeculae development when a diabetes state is present.