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The goal of the qNMR Summit is to take stock of the status quo and the recent developments in qNMR research and applications in a timely and accurate manner. It provides a platform for both advanced and novice qNMR practitioners to receive a well-rounded update and discuss potential qNMR-related applications and collaborations. For over a decade, scientists from academia, industry, nonprofit institutions, and governmental bodies have focused on the standardization of qNMR methodology, as well as its metrological and pharmacopeial utility. This paper reviews key content of qNMR Summits 1.0 to 4.0 and puts into perspective the outcomes and available transcripts of the October 2019 Summit 5.0, with attendees from the United States, Canada, Japan, Korea, and several European countries. Summit presentations focused on qNMR methodology in the pharmaceutical industry, advanced quantitation algorithms, and promising developments.
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Tecnologia , Canadá , Japão , Padrões de Referência , Estados UnidosRESUMO
Iron-based shape memory alloys are promising candidates for large-scale structural applications due to their cost efficiency and the possibility of using conventional processing routes from the steel industry. However, recently developed alloy systems like Fe-Mn-Al-Ni suffer from low recoverability if the grains do not completely cover the sample cross-section. To overcome this issue, here we show that small amounts of titanium added to Fe-Mn-Al-Ni significantly enhance abnormal grain growth due to a considerable refinement of the subgrain sizes, whereas small amounts of chromium lead to a strong inhibition of abnormal grain growth. By tailoring and promoting abnormal grain growth it is possible to obtain very large single crystalline bars. We expect that the findings of the present study regarding the elementary mechanisms of abnormal grain growth and the role of chemical composition can be applied to tailor other alloy systems with similar microstructural features.
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The ternary iron arsenide compound BaFe2As2 exhibits a structural phase transition from tetragonal to orthorhombic at a temperature of about 140 K. The twin lamellae arising below this transition temperature were studied in undoped single crystalline bulk and epitaxial thin film samples using electron backscatter diffraction in a scanning electron microscope equipped with a helium cryostat. Applying this technique on bulk single crystals a characteristic twin lamella size in the range of 0.1 µm up to a few µm was observed. In contrast, in epitaxially strained thin films the phase transition is not observed at temperatures above 19 K.
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PURPOSE: Talbot-Lau x-ray interferometry provides information about the scattering and refractive properties of an object - in addition to the object's attenuation features. Until recently, this method was ineligible for imaging human-sized objects as it is challenging to adapt Talbot-Lau interferometers (TLIs) to the relevant x-ray energy ranges. In this work, we present a preclinical Talbot-Lau prototype capable of imaging human-sized objects with proper image quality at clinically acceptable dose levels. METHODS: The TLI is designed to match a setup of clinical relevance as closely as possible. The system provides a scan range of 120 × 30 cm2 by using a scanning beam geometry. Its ultimate load is 100 kg. High aspect ratios and fine grid periods of the gratings ensure a reasonable setup length and clinically relevant image quality. The system is installed in a university hospital and is, therefore, exposed to the external influences of a clinical environment. To demonstrate the system's capabilities, a full-body scan of a euthanized pig was performed. In addition, freshly excised porcine lungs with an extrinsically provoked pneumothorax were mounted into a human thorax phantom and examined with the prototype. RESULTS: Both examination sequences resulted in clinically relevant image quality - even in the case of a skin entrance air kerma of only 0.3 mGy which is in the range of human thoracic imaging. The presented case of a pneumothorax and a reader study showed that the prototype's dark-field images provide added value for pulmonary diagnosis. CONCLUSION: We demonstrated that a dedicated design of a Talbot-Lau interferometer can be applied to medical imaging by constructing a preclinical Talbot-Lau prototype. We experienced that the system is feasible for imaging human-sized objects and the phase-stepping approach is suitable for clinical practice. Hence, we conclude that Talbot-Lau x-ray imaging has potential for clinical use and enhances the diagnostic power of medical x-ray imaging.
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Interferometria/métodos , Radiografia/métodos , Imagem Corporal Total/métodos , Raios X , Animais , Desenho de Equipamento , Humanos , Interferometria/instrumentação , Pulmão/diagnóstico por imagem , Modelos Anatômicos , Imagens de Fantasmas , Pneumotórax/diagnóstico por imagem , Doses de Radiação , Radiografia/instrumentação , Pele/diagnóstico por imagem , Suínos , Tórax/diagnóstico por imagem , Imagem Corporal Total/instrumentaçãoRESUMO
Different hardening strategies were evaluated regarding their potential to improve the mechanical biofunctionality of the cast and solution-treated low modulus ß-Ti alloy Ti 40Nb. The strategies are based on thermomechanical treatments comprised of different hot- and cold-rolling steps, as well as annealing treatments aiming at the successive exploitation of different hardening mechanisms (grain boundary hardening, work hardening and precipitation hardening). Quasi-static tensile testing revealed that grain refinement by one order of magnitude has only a small impact on improving the mechanical biofunctionality of Ti-40Nb. However, work hardening effectively improves the tensile strength by 30% to a value of 650MPa, while retaining Young׳s modulus at 60GPa. The α-phase precipitation hardening was verified to have an increasing effect on both, strength and Young׳s modulus. Thereby, the change of Young׳s modulus dominates the change of the strength, even at low α-phase fractions. The pseudo-elastic behavior of Ti 40Nb is discussed under consideration of the microstructural changes due to the thermomechanical treatment. The texture changes evolving upon cold-rolling markedly influence the recrystallization behavior. However, the present results do not show a significant effect of the texture on the mechanical properties of Ti-40Nb.
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Ligas/análise , Materiais Biocompatíveis/análise , Teste de Materiais , Módulo de Elasticidade , Resistência à Tração , TitânioRESUMO
Residual dipolar couplings are highly useful NMR parameters for calculating and refining molecular structures, dynamics, and interactions. For some applications, however, it is inevitable that the preferred orientation of a molecule in an alignment medium is calculated a priori. Several methods have been developed to predict molecular orientations and residual dipolar couplings. Being beneficial for macromolecules and selected small-molecule applications, such approaches lack sufficient accuracy for a large number of organic compounds for which the fine structure and eventually the flexibility of all involved molecules have to be considered or are limited to specific, well-studied liquid crystals. We introduce a simplified model for detailed all-atom molecular dynamics calculations with a polymer strand lined up along the principal axis as a new approach to simulate the preferred orientation of small to medium-sized solutes in polymer-based, gel-type alignment media. As is shown by a first example of strychnine in a polystyrene/CDCl3 gel, the simulations potentially enable the accurate prediction of residual dipolar couplings taking into account structural details and dynamic averaging effects of both the polymer and the solute.
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Géis , Simulação de Dinâmica Molecular , Processos EstocásticosRESUMO
The correlation between the microstructure and mechanical behavior during tensile loading of Ti68.8Nb13.6Al6.5Cu6Ni5.1 and Ti71.8Nb14.1Al6.7Cu4Ni3.4 alloys was investigated. The present alloys were prepared by the non-equilibrium processing applying relatively high cooling rates. The microstructure consists of a dendritic bcc ß-Ti solid solution and fine intermetallic precipitates in the interdendritic region. The volume fraction of the intermetallic phases decreases significantly with slightly decreasing the Cu and Ni content. Consequently, the fracture mechanism in tension changes from cleavage to shear. This in turn strongly enhances the ductility of the alloy and as a result Ti71.8Nb14.1Al6.7Cu4Ni3.4 demonstrates a significant tensile ductility of about 14% combined with the high yield strength of above 820 MPa already in the as-cast state. The results demonstrate that the control of precipitates can significantly enhance the ductility and yet maintaining the high strength and the low Young's modulus of these alloys. The achieved high bio performance (ratio of strength to Young's modulus) is comparable (or even superior) with that of the recently developed Ti-based biomedical alloys.
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Ligas/química , Metais/química , Módulo de Elasticidade , Microscopia Eletrônica , Resistência à Tração , Titânio/químicaRESUMO
With polyurethane (PU), a novel alignment medium for organic solvents is introduced and characterized, which is very robust and easy to produce on a large scale. Linear PU already constitutes an elastomer gel with several solvents based on its ability to form hydrogen bonds. Covalent cross-linking of the polymer with accelerated electrons provides an alignment medium with different properties. However, PU exhibits a number of undesired polymer signals in corresponding spectra, which ideally have to be removed spectroscopically. Within this context, we demonstrate the applicability of diffusion-filtered experiments for removal of the polymer signals. Example spectra for the usefulness of PU alignment media are provided for the common test molecules strychnine and norcamphor.
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Reagentes de Ligações Cruzadas/química , Difusão , Espectroscopia de Ressonância Magnética , Poliuretanos/química , Géis/química , Estrutura MolecularRESUMO
Having succeeded in the fabrication of epitaxial superconducting LaFeAsO(1-x)F(x) thin films we performed an extensive study of electrical transport properties. In the face of multiband superconductivity we can demonstrate that an anisotropic Ginzburg-Landau scaling of the angular dependent critical current densities can be adopted, although being originally developed for single band superconductors. In contrast with single band superconductors the mass anisotropy of LaFeAsO(1-x)F(x) is temperature dependent. A very steep increase of the upper critical field and the irreversibility field can be observed at temperatures below 6 K, indicating that the band with the smaller gap is in the dirty limit. This temperature dependence can be theoretically described by two dominating bands responsible for superconductivity. A pinning force scaling provides insight into the prevalent pinning mechanism and can be specified in terms of the Kramer model.
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Superconducting LaFeAsO1-xFx thin films were grown on single crystalline LaAlO3 substrates with critical temperatures (onset) up to 28 K. Resistive measurements in high magnetic fields up to 40 T reveal a paramagnetically limited upper critical field mu{0}H{c2}(0) around 77 T and a remarkable steep slope of -6.2 T K-1 near T{c}. From transport measurements we observed weak-link behavior in low magnetic fields and evidence for a broad reversible regime.
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We report upper critical field Bc2(T) data for disordered (arsenic-deficient) LaO0.9F0.1FeAs1-delta in a wide temperature and magnetic field range up to 47 T. Because of the large linear slope of Bc2 approximately -5.4 to -6.6 T/K near Tc approximately 28.5 K, the T dependence of the in-plane Bc2(T) shows a flattening near 23 K above 30 T which points to Pauli-limited behavior with Bc2(0) approximately 63-68 T. Our results are discussed in terms of disorder effects within [corrected] unconventional superconducting pairings.
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To simultaneously perform magnetization and magnetostriction measurements in high magnetic fields, a miniaturized device was developed that combines an inductive magnetometer with a capacitive dilatometer and, therefore, it is called "dilamagmeter." This combination of magnetic and magnetoelastic investigations is a new step to a complex understanding of solid state properties. The whole system can be mounted in a 12 mm clear bore of any cryostat usually used in nondestructive pulsed high field magnets. The sensitivity of both methods is about 10(-5) A m(2) for magnetization and 10(-5) relative changes in length for striction measurements. Measurements on a GdSi single crystal, which are corrected by the background signal of the experimental setup, agree well with the results of steady field experiments. All test measurements, which are up until now performed in the temperature range of 4-100 K, confirm the perfect usability and high stability in pulsed fields up to 50 T with a pulse duration of 10 ms.
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Heteronuclear residual dipolar one-bond couplings of organic molecules at natural abundance are most easily measured using t2 coupled HSQC spectra. However, inevitably mismatched transfer delays result in phase distortions due to residual dispersive antiphase coherences in such experiments. In this article, slightly modified t2 coupled HSQC experiments with clean inphase (CLIP) multiplets are introduced which also reduce the intensities of undesired long-range cross peaks. With the corresponding antiphase (CLAP) experiment, situations where alpha and beta components overlap can be resolved for all multiplicities in an IPAP manner. A comparison of the experiments using hard pulses and shaped broadband excitation and inversion pulses on the heteronucleus is given and potential spectral artefacts are discussed in detail.
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Ressonância Magnética Nuclear Biomolecular/métodos , Carbono/química , Isótopos de Carbono , Isótopos de Nitrogênio , Estricnina/química , Sacarose/químicaRESUMO
The measurement of residual dipolar couplings (RDCs), meanwhile a standard method for obtaining structural information in biomolecular NMR, requires partial alignment of the sample. Special demands on alignment media so far limit the applicability of this approach to small molecules in organic solvents. Major limitations are the free scalability of alignment and the suppression of residual signals of the alignment medium to allow effective measurement of low-concentration samples. Here, we present stretched poly(dimethylsiloxane) (PDMS) cross-linked by beta-rays as an alignment medium with no visible impurities in 1H NMR spectra but a single signal at approximately 0.1 ppm that can easily be removed by slightly modified water suppression methods. Besides the free scalability, its applicability to the measurement of RDCs in small molecules at low concentration is demonstrated on a approximately 12 mM sample of spiroindene. The induced alignment tensor in this case can be predicted reasonably well by a simplified model on the basis of steric interactions only.
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Dimetilpolisiloxanos/química , Espectroscopia de Ressonância Magnética/métodos , Géis , Modelos Moleculares , Solventes , EstereoisomerismoRESUMO
Parametric x rays (PXR) produced by bombarding silicon and diamond crystals with electrons of 30 to 87 MeV were detected at 180 degrees relative to the direction of the electron beam. It was found that the dependence of the intensity on the orientation of the crystal agrees with the predictions of the kinematical theory of PXR. The absolute intensity is twice as large as predicted. These findings can be explained considering dynamical effects that govern the x-ray crystal interaction. Additionally, x rays caused by self-diffracted transition radiation have been observed.
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Mevalonate kinase may play a key role in regulating cholesterol biosynthesis because its activity may be regulated via feedback inhibition by intermediates in the cholesterol biosynthetic pathway. To study the regulation of mevalonate kinase, the enzyme was purified to homogeneity from rat liver, and monospecific antibody against mevalonate kinase was prepared. The purified mevalonate kinase had a dimeric structure composed of identical subunits, and the Mr of the enzyme determined by gel chromatography was 86,000. Based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the subunit Mr was 39,900. The pI for mevalonate kinate was 6.2. The levels of mevalonate kinase protein and enzyme activity were determined in the livers of rats treated with either cholesterol-lowering agents (cholestyramine, pravastatin, and lovastatin) or with dietary modifications. Diets containing cholestyramine alone or cholestyramine and either pravastatin or lovastatin increased mevalonate kinase activity 3-6-fold. Mevalonate kinase activity decreased approximately 50% in rats treated with diets containing either 5% cholesterol or 5% cholesterol and 0.5% cholic acid. Fasting did not significantly change mevalonate kinase activity. The amount of mevalonate kinase protein in the liver was quantitated using immunoblots, and the changes in the levels of kinase activity induced by either drug treatment or by cholesterol feeding were correlated with similar changes in the levels of mevalonate kinase protein. Therefore, under these experimental conditions, mevalonate kinase activity in the liver was regulated principally by changes in the rates of enzyme synthesis and degradation.