A comprehensive approach to assess marine macro litter pollution and its impacts on corals in the Bangka Strait, North Sulawesi, Indonesia.

This is a comprehensive study showing the marine anthropogenic litter pollution within North Sulawesi, Indonesia. From an area of 2972 m2 that encompassed five sparsely populated locations, a total of 9421 litter items weighing 137 kg were collected. One location (Talisei North) contributed 50% of all collected litter items. Plastic litter always dominated with 96-99%. Litter was unevenly distributed across investigated areas reaching from the upper beach (3.6-30.1 items/m2) to the reef slope (0-0.03 items/m2). Litter composition and daily accumulation showed spatial-temporal dynamics, with upper beach areas displaying the overall highest accumulation rates. Reef micro-habitats were differently affected, with the reef moat and reef flat showing the highest litter concentrations, although litter amounts were much lower compared to the corresponding beaches. Branching corals, especially Porites cylindrica, were most affected by litter entanglement. Field experiments with P. cylindrica showed that attached plastic induced bleaching, necrosis, and algal overgrowth within five months.

Trashing our own "backyard" - Investigating dispersal and accumulation of floating litter from coastal, riverine, and offshore sources in the German Bight using a citizen science-based wooden drifter recapture approach.

Understanding marine debris dispersal through drift buoys and numerical modelling is one focus of litter pollution research that can identify particularly affected regions and aid targeted clean-up efforts. This study assessed the dispersal and accumulation of floating litter from coastal, riverine, and offshore sources in the German Bight, based on ~33,000 reports of wooden drifters deployed between 2016 and 2019. The majority (66.6%) released along the Lower Saxony coast and the rivers Ems, Weser, and Elbe were first reported within ≤25 km, indicating that coastal and riverine litter sources largely pollute adjacent shorelines. Drifters from coastal sites and release points near the river mouths dispersed similarly far, occasionally crossing distances comparable to those deployed at sea, reaching Scandinavia and Great Britain within a few days or weeks. Small-scale clustering occurred in <2% of all locations where drifters had been reported. These findings and limitations of the methodological approach are discussed.

Wasting the North Sea? - A field-based assessment of anthropogenic macrolitter loads and emission rates of three German tributaries.

Research into the scope of litter pollution in freshwater systems has shown similar levels to the marine and coastal environment. Global model estimates of riverine emission rates of anthropogenic litter are largely based on microplastic studies as long-term and holistic observations of riverine macroplastics are still scarce. This study therefore aims to contribute a detailed assessment of macrolitter in the transitional waters of three major North Sea tributaries: Ems, Weser, and Elbe. Litter surveys were carried out in four river compartments: along the embankment, on the river surface, in the water column, and on the river bed. The data revealed spatio-temporal variability and distinct pollution levels for each compartment. Beaches had the highest debris diversity and were significantly more littered than vegetated sites and harbors. Stony embankments were least polluted. Benthic litter levels appeared substantial despite rapid burial of objects being likely due to high suspended sediment loads. Two extrapolation approaches were tested to scale daily and annual litter emission quantities of surface- and subsurface-floating litter. Using the mean (median) litter item mass from water column samples, total annual mass discharges were calculated: ∼0.9 (0.2) t y-1 to ∼2.8 (0.5) t y-1 emitted via the Ems, ∼1.3 (0.2) t y-1 to ∼12.0 (1.9) t y-1 through the Weser, and ∼14.7 (2.4) t y-1 to ∼801 (128) t y-1 carried into the North Sea by the Elbe. These rates deviate considerably from previous model estimates of plastic loads discharged by these three rivers. Future studies should therefore ground-truth model estimates with more river-specific and long-term field observations. Overall, the estimated plastic debris discharge quantities account for <1% of the total mass of mismanaged plastic waste per catchment.

Wind, waves, tides, and human error? - Influences on litter abundance and composition on German North Sea coastlines: An exploratory analysis.

Being globally and locally prevalent, beach litter has been monitored at 29 sites along the German North Sea coastline. This study offers an exploratory analysis of data from 1991 to 2016. Schleswig-Holstein exhibited lower mean litter pollution levels than Lower Saxony, possibly because the locations in Lower Saxony are situated along the main coastal current, whereas the North Frisian Islands act as a barrier for the mainland sites in Schleswig-Holstein. Locations close to the Elbe estuary had significantly larger amounts of debris, likely receiving litter from marine and riverine sources. No clear overall pattern in litter abundance or composition could be detected. Significant quantitative similarities between debris types were inconsistent. The effect of wind, tides, and exposure appeared to be marginal. Recurring data inconsistencies, gaps, and outliers were partly attributed to human error. This could be reduced through hypothesis-driven monitoring with a simpler litter classification and continuous data checking.

Iron sulfide formation in young and rapidly-deposited permeable sands at the land-sea transition zone.

Organic-poor, permeable quartz sands are often present at land-sea transition zones in coastal regions. Yet, the biogeochemical cycles of carbon, sulfur, and iron are not well studied here. The aim of this work was, therefore, to improve our understanding regarding the chemical processes in these prominent coastal sediments. A 10 m core was collected at a dune base of the barrier island Spiekeroog, Germany, for this purpose. Additionally, groundwater was sampled from a multi-level well for one year to record seasonal hydrochemical variations. Methods included the analyses of geochemical (total carbon, total inorganic carbon, reactive iron, total sulfur, reduced inorganic sulfur) and hydrochemical parameters (field parameters, major ions, DOC, and molecular compositions of DOM), as well as stable sulfur isotopes (δ34S-sulfate, -sulfide, -total reduced inorganic sulfur). Moreover, optically stimulated luminescence (OSL) dating was applied. Results show that the core sediments are very young (<500 a) and were rapidly deposited. They are characterized by remarkably low contents of organic carbon (<0.1% dw.), reactive iron (~10 mmol/kg), and iron sulfides (<3 mmol/kg). Groundwater salinities were low in the top core sediments and increased at depth during most times of the year. However, the sampling site is subject to (seasonally) varying salinities, which could be linked to the biogeochemical cycles. For instance, the infiltration of seawater-derived labile DOM during inundation events drives microbial respiration besides sedimentary organic matter. Oxygen and nitrate were the dominant electron acceptors for the decomposition of organic matter in near-surface groundwater, while sulfate reduction was constrained to the lower brackish sediments. Here, authigenic pyrite formation was inferred based on the detection of dissolved sulfide, intact pyrite framboids, and matching stable sulfur isotope signatures of dissolved and solid sulfides. We concluded that the extremely low organic carbon contents limit pyrite formation in the organic-poor, permeable quartz sands.

Impact of Extraction Methods on the Detectable Protein Complement of Metaproteomic Analyses of Marine Sediments.

Metaproteomic analysis targets proteins, the catalytic entities in the habitat, thereby providing direct insights into the metabolic activity of the community studied. A major challenge still remaining for metaproteomics is the effective and comprehensive extraction of proteins from environmental samples, due to their high complexity with respect to organismic diversity and abundance range. Moreover, in certain habitats, the inherent matrix may interfere with protein extraction. In recent years, several studies reported different protein extraction methods for soils known for their complex geochemistry, but only three analyzed marine sediments that generally comprise different though similarly complex geochemistry. In this study, the impact of four different extraction methods was investigated for coastal North Sea and deep sea Pacific Ocean sediments. The extraction methods comprised (i) phenol, (ii) SDS, (iii) a mixture of SDS and phenol, and (iv) urea and thiourea. Prior to extraction, a cell and protein standard (CPS) was added to the sediment samples to trace recovery of proteins from different subcellular locations as well as dissolved BSA. While each extraction method detected distinct peptide complements, SDS-phenol extraction generally achieved highest protein yield and most comprehensive CPS protein identification. Application of two different methods was shown to further improve proteome coverage.