RESUMO
We studied the effect of KE and AED peptides on the expression of sirtuin-1, sirtuin-6, collagen I, cytokines (IL-1, TGF-ß), and transcription factor NF-κB in human skin fibroblasts during their replicative aging. Immunocytochemical analysis and confocal microscopy showed that KE peptide reduces the synthesis of factors of the inflammatory response IL-1, NF-κB, and TGF-ß and stimulates the synthesis of sirtuin-6. KE peptide normalizes the immunological function of human skin fibroblasts during their aging. AED peptide activates the synthesis of sirtuin-1, sirtuin-6, and collagen I in human skin fibroblasts during their replicative aging, which attests to its geroprotective effect.
Assuntos
Antioxidantes/farmacologia , Fibroblastos/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Fatores Imunológicos/farmacologia , Oligopeptídeos/farmacologia , Antioxidantes/síntese química , Senescência Celular/efeitos dos fármacos , Colágeno Tipo I/genética , Colágeno Tipo I/imunologia , Feminino , Fibroblastos/citologia , Fibroblastos/imunologia , Humanos , Fatores Imunológicos/síntese química , Interleucina-1/genética , Interleucina-1/imunologia , NF-kappa B/genética , NF-kappa B/imunologia , Oligopeptídeos/síntese química , Cultura Primária de Células , Transdução de Sinais , Sirtuína 1/genética , Sirtuína 1/imunologia , Sirtuínas/genética , Sirtuínas/imunologia , Fator de Crescimento Transformador beta/genética , Fator de Crescimento Transformador beta/imunologiaRESUMO
Skin aging is one of the topical issues in modern gerontocosmetology. Application of cosmetic products with short peptides is a promising measure for retardation of skin aging. This research is aimed at investigation of KE (Lys-Glu, Vilon) dipeptide influence on the expression of markers of aging in human skin fibroblasts in vitro. Collagen type I and sirtuin-6 expression in «young¼ and «old¼ skin cell fibroblasts cultures was studied using immunofluorescence confocal microscopy method. The areas of expression of collagen type I and sirtuin-6 are known to decrease in skin fibroblasts with aging by 3,5 and 3,6 times accordingly. KE dipeptide increases collagen type I expression area in «old¼ skin fibroblasts cultures by 83%. KE dipeptide increases expression area of sirtuin-6 in «young¼ and «old¼ skin fibroblasts cultures by 1,6 and 2,6 times correspondingly. Thus, KE dipeptide promotes functional activity of skin fibroblasts and inhibits their aging.
Assuntos
Senescência Celular/efeitos dos fármacos , Colágeno Tipo I/metabolismo , Dipeptídeos/farmacologia , Fibroblastos/efeitos dos fármacos , Ozônio/uso terapêutico , Substâncias Protetoras/farmacologia , Sirtuínas/metabolismo , Envelhecimento da Pele/efeitos dos fármacos , Idoso , Células Cultivadas , Terapia Combinada/métodos , Fibroblastos/fisiologia , Humanos , Microscopia Confocal/métodos , Microscopia de Fluorescência/métodosRESUMO
The review of the peptides used in dermatocosmetology is represented. The purpose of this review is a search of results of the basic scientific researches confirming or disproving efficiency and/or safety of peptide molecules used in a modern dermatocosmetology. Questions of forms of application, biological availability, reliability of the rendered anti-aging effects are considered.
Assuntos
Técnicas Cosméticas , Dermatologia/métodos , Peptídeos/farmacologia , Envelhecimento da Pele , Disponibilidade Biológica , Fármacos Dermatológicos/farmacologia , Humanos , Reprodutibilidade dos Testes , Envelhecimento da Pele/efeitos dos fármacos , Envelhecimento da Pele/patologia , Envelhecimento da Pele/fisiologiaRESUMO
Two types of lens photography are compared in the same patients: photography according to Schaimpflüger and reproduction biomicrophotography with subsequent densitometry of the negatives. The authors find Schaimpflüger's photography preferable, its advantages consisting in convenience of the procedure and possibility to make standard pictures. An experienced worker can make high-quality images of the lens optic section with a slit photographic lamp, this permitting an objective assessment of changes in the optic densities of different lenticular laminae.
Assuntos
Cristalino , Fotografação/métodos , Densitometria , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
Fluorescein movements in the lens laminae of isolated porcine eyes were examined by biomicroscopy. The stain disseminated in two directions: from the lens nucleus to capsules and fluorescein release into the intraocular fluid and from the outer laminae of the equatorial zone to the nucleus. Lecozyme, a proteolytic agent, accelerated fluorescein movement in both directions. The method suggested by the authors permits an objective assessment of fluorescein movement in the lens, which appears to reflect the processes of extracellular fluid ultrafiltration in the lens. The data evidence the possibility of effecting the extracellular intralenticular fluid microcirculation by proteolytic enzymes. The suggested technique may be useful in studies of cataract pathogenesis and of anticataract action of various drugs.
