Multifunctional glycoprotein coatings improve the surface properties of highly oxygen permeable contact lenses.

To achieve and maintain good operability of medical devices while reducing putative side effects for the patient, a promising strategy is to tailor the surface properties of such devices as they critically dictate the tissue compatibility and the biofouling behavior. Indeed, those properties can be strongly improved by generating mucin coatings on such medical devices. However, using coatings on optical systems, e.g., contact lenses, comes with various challenges: here, the geometrical and optical characteristics of the lens may not be compromised by either the coating process or the coating itself. In this study, we show how mucin macromolecules can be attached onto the surfaces of rigid, gas permeable contact lenses while maintaining all critical lens parameters. We demonstrate that the generated coatings improve the surface wettability (contact angles are reduced from 105° to 40° and liquid film break-up times are increased from <1 s to 31 s) and prevent tribological damage to corneal tissue. Additionally, such coatings are highly transparent (transmission values above 98 % compared to an uncoated sample are reached) and efficiently reduce lipid deposition to the lens surface by 90 % but fully maintain the geometrical and mechanical properties of the lenses. Thus, such mucin coatings could also be highly beneficial for other optical systems that are used in direct contact with tissues or body fluids.

Highly Transparent Covalent Mucin Coatings Improve the Wettability and Tribology of Hydrophobic Contact Lenses.

A stable, good coverage of the corneal tissue by the tear film is essential for protecting the eye. Contact lenses, however, constitute a foreign body that separates the tear film into two thinner layers, which are then more vulnerable toward disruption. This effect is even more pronounced if the contact lenses possess an insufficient surface wettability, which, in addition to friction, is suggested to be linked to discomfort and damage to the ocular surface. In this study, we establish covalent surface coatings with mucin macromolecules to overcome this issue for pure silicone contact lenses. This material class, which outperforms state-of-the-art silicone hydrogels in terms of oxygen permeability, is not yet used for commercial contact lens applications, which is due to its strongly hydrophobic surface characteristics. The applied process stably attaches a transparent mucin layer onto the contact lenses and thereby establishes hydrophilic surfaces that not only prevent lipid adsorption but also interact very well with liquid environments. Most importantly, however, we show that those mucin coatings are indeed able to prevent wear formation on corneal tissue that is subjected to the tribological stress applied by a contact lens. Our results open up great possibilities for a variety of hydrophobic materials that are, to date, not suitable for a contact lens application. Furthermore, the ability of mucin coatings to reduce wear in a tissue/synthetic material contact might be also beneficial for other biomedical applications.

In vitro adhesion of Acanthamoeba castellanii to soft contact lenses depends on water content and disinfection procedure.

PURPOSE: To compare the potential of different soft contact lenses to be contaminated with Acanthamoeba castellanii as a function of material parameters and cleaning procedures. METHODS: Different unworn soft hydrogel and silicone hydrogel contact lenses were incubated with human pathogenic A. castellanii. The adhesion of the acanthamoebae was investigated on the contact lenses and put into relation to their material parameters. The efficacy of a recommended contact lens cleaning procedure in reducing A. castellanii adhesion was investigated. RESULTS: We found that material parameters such as elastic modulus, silicone content, ionic properties and swelling do not influence the adhesion of acanthamoebae to soft contact lenses. A material parameter that influenced adhesion significantly was the water content of the lens. With increasing water content, the adhesion of acanthamoebae increased. By following the cleaning instructions of the manufacturer the contamination of the lenses with A. castellanii could be reduced to a minimum, as shown both on contact lenses and in control experiments. CONCLUSION: With this study we show that for the tested lenses, the adhesion of A. castellanii to contact lenses is independent of the silicone content of the lens, but depends nonlinearly on the water content of the lens. Furthermore, we demonstrate that applying proper lens cleaning procedures minimizes the risk of acanthamoebae adhesion to contact lenses.