Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 19 de 19
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
2.
PLoS One ; 18(8): e0289279, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37527243

RESUMO

Single-cell transcriptomics is essential for understanding biological variability among cells in a heterogenous population. Acquiring high-quality single-cell sequencing data from a tissue sample has multiple challenges including isolation of individual cells as well as amplification of the genetic material. Commercially available techniques require the isolation of individual cells from a tissue through extensive manual manipulation before single cell sequence data can be acquired. However, since cells within a tissue have different dissociation constants, enzymatic and mechanical manipulation do not guarantee the isolation of a homogenous population of cells. To overcome this drawback, in this research we have developed a revolutionary approach that utilizes a fully automated nanopipette technology in combination with magnetic nanoparticles to obtain high quality sequencing reads from individual cells within an intact tissue thereby eliminating the need for manual manipulation and single cell isolation. With the proposed technology, it is possible to sample an individual cell within the tissue multiple times to obtain longitudinal information. Single-cell RNAseq was achieved by aspirating only1-5% of sub-single-cell RNA content from individual cells within fresh frozen tissue samples. As a proof of concept, aspiration was carried out from 22 cells within a breast cancer tissue slice using quartz nanopipettes. The mRNA from the aspirate was then selectively captured using magnetic nanoparticles. The RNAseq data from aspiration of 22 individual cells provided high alignment rates (80%) with 2 control tissue samples. The technology is exceptionally simple, quick and efficient as the entire cell targeting and aspiration process is fully automated.


Assuntos
Perfilação da Expressão Gênica , RNA , RNA/genética , RNA Mensageiro/genética , Separação Celular , Perfilação da Expressão Gênica/métodos , Análise de Sequência de RNA/métodos , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Análise de Célula Única/métodos
3.
Animals (Basel) ; 13(5)2023 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-36899712

RESUMO

Global climate change has become a trend and is one of the main factors affecting biodiversity patterns and species distributions. Many wild animals adapt to the changing living environment caused by climate change by changing their habitats. Birds are highly sensitive to climate change. Understanding the suitable wintering habitat of the Eurasian Spoonbill (Platalea leucorodia leucorodia) and its response to future climatic change is essential for its protection. In China, it was listed as national grade II key protected wild animal in the adjusted State List of key protected wild animals in 2021, in Near Threatened status. Few studies on the distribution of the wintering Eurasian Spoonbill have been carried out in China. In this study, we simulated the suitable habitat under the current period and modeled the distribution dynamics of the wintering Eurasian Spoonbill in response to climate change under different periods by using the MaxEnt model. Our results showed that the current suitable wintering habitats for the Eurasian Spoonbill are mainly concentrated in the middle and lower reaches of the Yangtze River. Distance from the water, precipitation of the driest quarter, altitude, and mean temperature of the driest quarter contributed the most to the distribution model for the wintering Eurasian Spoonbill, with a cumulative contribution of 85%. Future modeling showed that the suitable distribution of the wintering Eurasian Spoonbill extends to the north as a whole, and the suitable area shows an increasing trend. Our simulation results are helpful in understanding the distribution of the wintering Eurasian Spoonbill under different periods in China and support species conservation.

4.
Luminescence ; 38(2): 109-115, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36544245

RESUMO

Reactive oxygen species (ROS) are usually produced in rice under aerobic environmental conditions, resulting in peroxidative changes in polyunsaturated fatty acids, and affecting the deterioration of rice during storage. In addition, as an important enzyme that participates in removing ROS, peroxidase is also present in rice, and takes part in protecting rice from attack by ROS. Moreover, loss of peroxidase activity may give rise to rice deterioration during storage. Therefore, measuring peroxidase activity makes it possible to ascertain the freshness of rice. In addition, peroxidase can also catalyze the luminol-hydrogen peroxide system. Based on this, in this work we established a new chemiluminescence (CL) method that was used to detect the freshness of stored rice. Under optimal experimental conditions, we showed that the freshness of rice can be measured using this CL method. This study is the first to detect the freshness of rice using a CL method, opening up a novel direction for the application of CL.


Assuntos
Oryza , Espécies Reativas de Oxigênio , Luminescência , Medições Luminescentes/métodos , Luminol , Peroxidase , Peróxido de Hidrogênio
5.
Clin Chem ; 67(3): 534-542, 2021 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-33393992

RESUMO

BACKGROUND: Liquid biopsy circulating tumor DNA (ctDNA) mutational analysis holds great promises for precision medicine targeted therapy and more effective cancer management. However, its wide adoption is hampered by high cost and long turnaround time of sequencing assays, or by inadequate analytical sensitivity of existing portable nucleic acid tests to mutant allelic fraction in ctDNA. METHODS: We developed a ctDNA Epidermal Growth Factor Receptor (EGFR) mutational assay using giant magnetoresistive (GMR) nanosensors. This assay was validated in 36 plasma samples of non-small cell lung cancer patients with known EGFR mutations. We assessed therapy response through follow-up blood draws, determined concordance between the GMR assay and radiographic response, and ascertained progression-free survival of patients. RESULTS: The GMR assay achieved analytical sensitivities of 0.01% mutant allelic fraction. In clinical samples, the assay had 87.5% sensitivity (95% CI = 64.0-97.8%) for Exon19 deletion and 90% sensitivity (95% CI = 69.9-98.2%) for L858R mutation with 100% specificity; our assay detected T790M resistance with 96.3% specificity (95% CI = 81.7-99.8%) with 100% sensitivity. After 2 weeks of therapy, 10 patients showed disappearance of ctDNA by GMR (predicted responders), whereas 3 patients did not (predicted nonresponders). These predictions were 100% concordant with radiographic response. Kaplan-Meier analysis showed responders had significantly (P < 0.0001) longer PFS compared to nonresponders (N/A vs. 12 weeks, respectively). CONCLUSIONS: The GMR assay has high diagnostic sensitivity and specificity and is well suited for detecting EGFR mutations at diagnosis and noninvasively monitoring treatment response at the point-of-care.


Assuntos
Técnicas Biossensoriais , Carcinoma Pulmonar de Células não Pequenas , DNA Tumoral Circulante/genética , Análise Mutacional de DNA/métodos , Monitoramento de Medicamentos/métodos , Receptores ErbB/genética , Neoplasias Pulmonares , Acrilamidas/uso terapêutico , Idoso , Compostos de Anilina/uso terapêutico , Carcinoma Pulmonar de Células não Pequenas/diagnóstico , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Carcinoma Pulmonar de Células não Pequenas/genética , Feminino , Humanos , Biópsia Líquida , Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/genética , Masculino , Pessoa de Meia-Idade , Mutação , Análise de Sequência com Séries de Oligonucleotídeos , Inibidores de Proteínas Quinases/uso terapêutico , Proteínas Tirosina Quinases/antagonistas & inibidores
6.
ACS Nano ; 6(8): 6862-9, 2012 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-22857784

RESUMO

Early detection and targeted therapy are two major challenges in the battle against cancer. Novel imaging contrast agents and targeting approaches are greatly needed to improve the sensitivity and specificity of cancer theranostic agents. Here, we implemented a novel approach using a magnetic micromesh and biocompatible fluorescent magnetic nanoparticles (FMN) to magnetically enhance cancer targeting in living subjects. This approach enables magnetic targeting of systemically administered individual FMN, containing a single 8 nm superparamagnetic iron oxide core. Using a human glioblastoma mouse model, we show that nanoparticles can be magnetically retained in both the tumor neovasculature and surrounding tumor tissues. Magnetic accumulation of nanoparticles within the neovasculature was observable by fluorescence intravital microscopy in real time. Finally, we demonstrate that such magnetically enhanced cancer targeting augments the biological functions of molecules linked to the nanoparticle surface.


Assuntos
Corantes Fluorescentes/química , Glioblastoma/patologia , Nanopartículas de Magnetita , Microscopia de Fluorescência/métodos , Nanocápsulas/química , Animais , Linhagem Celular Tumoral , Humanos , Campos Magnéticos , Teste de Materiais , Camundongos , Camundongos SCID , Nanocápsulas/ultraestrutura , Tamanho da Partícula
7.
Appl Biochem Biotechnol ; 166(6): 1604-14, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22328250

RESUMO

Enzyme-linked immunosorbent assay (ELISA), horseradish peroxidase (HRP)-catalyzed fluorescent reaction, and oxalate chemiluminescence analysis have been combined to develop a highly sensitive, simple, and rapid method for analysis of bovine serum albumin (BSA) based on a pair of specific monoclonal antibodies in vaccines. A typical "sandwich type" immunoassay was used. Reaction of 3-(4-hydroxyphenyl propionate) (PHPPA) with hydrogen peroxide-urea, catalyzed by HRP, produced fluorescence of 3-(4-hydroxyphenyl propionate) dimer, which was detected by chemiluminescence analysis with the bis(2,4,6-trichlorophenyl)oxalate (TCPO)-H(2)O(2)-glyoxaline-PHPPA dimer chemiluminescent system. This method exhibited high performance with a linear correlation between response and amount of bovine serum albumin (BSA) in the range 0.1 to 100.0 ng mL(-1) (r = 0.9988), and the detection limit was 0.03 ng mL(-1) (S/N = 3). Intra- and interassay coefficient variations were all lower than 9.0% at three concentrations (1.0, 20.0, and 80.0 ng mL(-1)). The proposed method has been used for successful analysis of the amount of residual BSA in vaccines. The results obtained compared well with those obtained by conventional colorimetric ELISA and luminol chemiluminescent ELISA.


Assuntos
Anticorpos Monoclonais/química , Imunoensaio/métodos , Medições Luminescentes/métodos , Soroalbumina Bovina/química , Vacinas/química , Animais , Bovinos , Ensaio de Imunoadsorção Enzimática/métodos , Epitopos/química , Peroxidase do Rábano Silvestre/química , Peróxido de Hidrogênio/química , Luminescência , Oxalatos/química , Sensibilidade e Especificidade , Soroalbumina Bovina/análise , Temperatura , Fatores de Tempo , Ureia/química , Vacinas/análise
8.
Biosens Bioelectron ; 26(9): 3958-61, 2011 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-21470843

RESUMO

A highly sensitive and stable sandwich fluorescence immunoassay for the quantitative detection of residual BSA in vaccines based on the labels of the functionalized fluorescent core-shell silica nanoparticles and laser-induced fluorescence millimeter sensor array detection platform has been developed. On a glass slide with low fluorescence background, capture antibody against BSA was immobilized, after BSA was captured, another identify antibody against BSA which was labeled with the new fluorescent silica nanoparticles was used to recognize the BSA. The fluorescence issued from the fluorescent silica nanoparticles was successfully detected by the laser induced fluorescence millimeter sensor assay detection platform which was made by us. This method exhibited high performance with a linear correlation between response and amount of BSA in the range 1.0-100 ng/mL and the detection limit was 0.3 ng/mL (3σ). The relative standard deviation (R.S.D.) was 6.7% at the concentration of 20 ng/mL for 5 parallel measurements of BSA.


Assuntos
Técnicas Biossensoriais , Soroalbumina Bovina/isolamento & purificação , Vacinas/química , Animais , Bovinos , Fluorescência , Imunoensaio , Limite de Detecção , Nanopartículas/química , Soroalbumina Bovina/química , Dióxido de Silício/química
9.
Angew Chem Int Ed Engl ; 48(9): 1620-4, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19156803

RESUMO

Direct protein functionalization provides synthetic antiferromagnetic nanoparticles with high chemical specificity and multifunctionality. These nanoparticle-protein conjugates function as improved magnetic labels for biological detection experiments, and exhibit tunable responses to a small external magnetic field gradient, thus allowing the observation of distinctive single nanoparticle motion.


Assuntos
Nanopartículas/química , Proteínas/química , Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais , Magnetismo , Microscopia Eletrônica de Varredura , Proteínas/síntese química
10.
Artigo em Chinês | MEDLINE | ID: mdl-20108782

RESUMO

OBJECTIVE: For the birth, to improve the quality of the population, to explore the prevention and treatment of early pregnancy TORCH infection, and treatment of patients with positive eugenics and guidance. METHODS: Enzyme-linked immunosorbent assay (ELISA) testing of all the objects in the peripheral blood-money pathogen-specific antibodies LgM. Person in charge of testing. In strict accordance with the instructions. Reagents from Shanghai magnolia biotechnology institute. RESULT: The total number of 319 cases of positive, with a total infection rate: 3.28%, TOX-IgM, RV-IgM, CMV-IgM, HSV (II)-IgM infection rate of 0.103 percent, 2.64 percent and 0.309 percent, 0.237 percent; 319 cases of TORCH infected persons are to receive treatment for the treatment of wrap, with a total negative rate of 97.49 percent. CONCLUSION: Detection of TORCH infections to ensure early diagnosis, early treatment and early prevention is necessary.


Assuntos
Citomegalovirus/imunologia , Complicações Infecciosas na Gravidez/parasitologia , Complicações Infecciosas na Gravidez/virologia , Vírus da Rubéola/imunologia , Simplexvirus/isolamento & purificação , Toxoplasma/imunologia , Anticorpos Antiprotozoários/sangue , Anticorpos Antiprotozoários/imunologia , Anticorpos Antivirais/sangue , Anticorpos Antivirais/imunologia , Antiprotozoários/uso terapêutico , Antivirais/uso terapêutico , Citomegalovirus/isolamento & purificação , Feminino , Humanos , Imunoglobulina M/sangue , Imunoglobulina M/imunologia , Gravidez , Complicações Infecciosas na Gravidez/tratamento farmacológico , Complicações Infecciosas na Gravidez/imunologia , Vírus da Rubéola/efeitos dos fármacos , Vírus da Rubéola/isolamento & purificação , Simplexvirus/efeitos dos fármacos , Simplexvirus/imunologia , Toxoplasma/efeitos dos fármacos , Toxoplasma/isolamento & purificação , Resultado do Tratamento
11.
J Magn Magn Mater ; 321(10): 1452-1458, 2009 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-20161001

RESUMO

Optical observations of 100 nm metallic magnetic nanoparticles are used to study their magnetic field induced self assembly. Chains with lengths of tens of microns are observed to form within minutes at nanoparticle concentrations of 10(10) per mL. Chain rotation and magnetophoresis are readily observed, and SEM reveals that long chains are not simple single particle filaments. Similar chains are detected for several 100 nm commercial bio-separation nanoparticles. We demonstrate the staged magnetic condensation of different types of nanoparticles into composite structures and show that magnetic chains bind to immunomagnetically labeled cells, serving as temporary handles which allow novel magnetic cell manipulations.

12.
Nano Lett ; 7(1): 179-82, 2007 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-17212460

RESUMO

In this paper, we report the development of rod-shaped semiconductor nanocrystals (quantum rods) as fluorescent biological labels. Water-soluble biocompatible quantum rods have been prepared by surface silanization and applied for nonspecific cell tracking as well as specific cellular targeting. Quantum rods are brighter single molecule probes as compared to quantum dots. They have many potential applications as biological labels in situations where their properties offer advantages over quantum dots.


Assuntos
Corantes Fluorescentes , Teoria Quântica , Semicondutores
13.
Nano Lett ; 6(4): 843-7, 2006 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-16608295

RESUMO

The photoluminescence dynamics of colloidal CdSe/ZnS/streptavidin quantum dots were studied using time-resolved single-molecule spectroscopy. Statistical tests of the photon-counting data suggested that the simple "on/off" discrete state model is inconsistent with experimental results. Instead, a continuous emission state distribution model was found to be more appropriate. Autocorrelation analysis of lifetime and intensity fluctuations showed a nonlinear correlation between them. These results were consistent with the model that charged quantum dots were also emissive, and that time-dependent charge migration gave rise to the observed photoluminescence dynamics.


Assuntos
Compostos de Cádmio/química , Medições Luminescentes , Modelos Químicos , Pontos Quânticos , Compostos de Selênio/química , Sulfetos/química , Compostos de Zinco/química , Compostos de Cádmio/efeitos da radiação , Simulação por Computador , Luz , Teste de Materiais , Compostos de Selênio/efeitos da radiação , Sulfetos/efeitos da radiação , Compostos de Zinco/efeitos da radiação
14.
Curr Opin Neurobiol ; 15(5): 568-75, 2005 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-16150591

RESUMO

Conventional organic fluorophores suffer from poor photo stability, narrow absorption spectra and broad emission spectra. Semiconductor nanocrystals, however, are highly photo-stable with broad absorption spectra and narrow size-tunable emission spectra. Recent advances in the synthesis of these materials have resulted in the generation of bright, sensitive, extremely photo-stable and biocompatible semiconductor fluorophores. Commercial availability facilitates their application in a variety of unprecedented biological experiments, including multiplexed cellular imaging, long-term in vitro and in vivo labeling, deep tissue structure mapping and single particle investigation of dynamic cellular processes. Semiconductor nanocrystals are one of the first examples of nanotechnology enabling a new class of biomedical applications.


Assuntos
Diagnóstico por Imagem/métodos , Nanotecnologia/métodos , Pontos Quânticos , Semicondutores , Animais , Cristalização , Humanos
15.
J Am Chem Soc ; 126(35): 10832-3, 2004 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-15339154

RESUMO

Nanostructures of colloidal CdSe/ZnS core/shell quantum dots (QDs) surrounded by a discrete number of Au nanoparticles were generated via DNA hybridization and purified by gel electrophoresis. Statistics from TEM analysis showed a high yield of designed structures. The distance between Au particles and QD, the number of Au around the central QD, and the size of Au and QD can be adjusted. Rationally designed structures such as these hold great promise for researching the physical interactions between semiconductor and Au nanoparticles and for developing more efficient nanoprobes.


Assuntos
DNA/química , Ouro/química , Pontos Quânticos , Eletroforese , Nanotecnologia/métodos , Hibridização de Ácido Nucleico
16.
Anal Chem ; 75(18): 4766-72, 2003 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-14674452

RESUMO

We report two cDNA microarray-based applications of DNA-nanocrystal conjugates, single-nucleotide polymorphism (SNP) and multiallele detections, using a commercial scanner and two sets of nanocrystals with orthogonal emissions. We focus on SNP mutation detection in the human p53 tumor suppressor gene, which has been found to be mutated in more than 50% of the known human cancers. DNA-nanocrystal conjugates are able to detect both SNP and single-base deletion at room temperature within minutes, with true-to-false signal ratios above 10. We also demonstrate microarray-based multiallele detection, using hybridization of multicolor nanocrystals conjugated to two sequences specific for the hepatitis B and hepatitis C virus, two common viral pathogens that inflict more than 10% of the population in the developing countries worldwide. The simultaneous detection of multiple genetic markers with microarrays and DNA-nanocrystal conjugates has no precedent and suggests the possibility of detecting an even greater number of bacterial or viral pathogens simultaneously.


Assuntos
Alelos , DNA/genética , Análise de Sequência com Séries de Oligonucleotídeos , Polimorfismo de Nucleotídeo Único , Cristalização , Hibridização de Ácido Nucleico , Sensibilidade e Especificidade , Espectrometria de Fluorescência
17.
Beijing Da Xue Xue Bao Yi Xue Ban ; 35(5): 449-52, 2003 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-14601296

RESUMO

OBJECTIVE: To evaluate the role played by mesolimbic dopaminergic system in the reinstatement of drug-seeking behavior induced by priming injections of morphine. METHODS: After the extinguishment of morphine conditioned place preference (CPP), low-dose catecholaminergic neurotoxin 6-hydroxydopamine (6-OHDA) was bilaterally injected into ventral tegmental area (VTA, 1 g.L-1) and nucleus accumbens (NAc, 5 g.L-1) before being primed with low-dose morphine. RESULTS: The effects of drug-priming to induce reinstatement of morphine CPP could be completely abolished by 6-OHDA microinjected into VTA to damage the perikaryon of dopaminergic neurons, or into NAc to lesion the terminal field of the dopaminergic pathway. CONCLUSION: The functional integrality of the mesolimbic dopaminergic system is indispensable for drug priming-induced reinstatement of conditioned place preference.


Assuntos
Comportamento de Procura de Droga , Morfina , Oxidopamina , Animais , Condicionamento Operante , Dopamina , Núcleo Accumbens , Ratos
18.
Brain Res ; 950(1-2): 1-9, 2002 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-12231223

RESUMO

We have previously shown with a model of morphine-induced conditioned place preference (CPP) that a brief exposure to footshock stress or a priming dose of morphine could reactivate drug-seeking behavior after a long drug-free period. The present study was designed to examine the possible role of certain brain areas in such a reactivation. After the rats were successfully trained with morphine (4 mg/kg, i.p.) through a CPP paradigm (10 sessions of daily pairing of morphine with one of the two compartments), different parts of nucleus accumbens (NAc), ventral tegmental area (VTA), and central (Ce) or lateral (La) nucleus of amygdala were lesioned with a DC current passing through the respective location. After a 9-day abstinence period, random intermittent footshock (DC square wave, 0.5 mA, 0.5 s width, off time 10-70 s) or drug priming (morphine 0.25 mg/kg, s.c.) reactivated the place preference in sham lesion rats. However, the effect of drug priming could be completely abolished by lesions placed either at VTA, or the majority or shell part, but not the core of NAc. On the other hand, the effect of footshock stressor could be eliminated by a lesion placed at Ce but not La. These results suggest that, while both drug priming and footshock stress are effective in reactivating drug-seeking behavior, they might work through different neurochemical mechanisms and anatomical pathways.


Assuntos
Comportamento Aditivo/psicologia , Encéfalo/efeitos dos fármacos , Condicionamento Psicológico/efeitos dos fármacos , Morfina/farmacologia , Estresse Fisiológico/psicologia , Tonsila do Cerebelo/efeitos dos fármacos , Tonsila do Cerebelo/fisiologia , Animais , Encéfalo/fisiologia , Condicionamento Psicológico/fisiologia , Masculino , Núcleo Accumbens/efeitos dos fármacos , Núcleo Accumbens/fisiologia , Ratos , Ratos Sprague-Dawley , Área Tegmentar Ventral/efeitos dos fármacos , Área Tegmentar Ventral/fisiologia
19.
Chemistry ; 8(10): 2239-47, 2002 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-12012407

RESUMO

Controlled synthesis of transition metal complexes with mixed ligands has led to two new compounds with the same empirical formula [Fe(N3)2(4,4'-bpy)] (4,4'-bpy=4,4'- bipyridine). The compound 2D-[Fe(N3)2(4,4'-bpy)] (I) contains end-on (EO) bridging azido ligands. It crystallizes in the orthorhombic crystal system, space group Cmmm (No. 65): a=11.444(2) A, b=15.181(3) A, c=3.458(1) A, V=600.8(2) A(3), and Z=2. The compound 3D-[Fe(N3)2(4,4'-bpy)] (II) contains end-to-end (EE) azido bridges. It belongs to the tetragonal crystal system, space group P4(1)2(1)2 (No. 92): a=8.132(1) A, b=8.132(1) A, c=16.708(3) A, V=1104.9(5) A(3), and Z=4. Crystals of I and II have been grown by the diffusion method. Phase-pure samples of both compounds have been obtained by means of an optimal solution synthesis. Spontaneous long-range magnetic ordering was found in both I and II, with I being a metamagnet, and II being a ferromagnet. For I, in the low-field region, multiple transitions at TN1=20 K and TN2=5 K were observed, and these indicated the existence of Fe moment reorientation. Heat capacity measurements on II confirmed ferromagnetic transition at TC=20 K.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA