High-temperature responses of Myzus persicae and its parasitoid Aphidius gifuensis in relation to heat level, duration and developmental stage.

BACKGROUND: Understanding how parasitoids respond to temperature is crucial for improving biological control strategies under the context of global warming. This study examined the suitability of Myzus persicae and its parasitoid Aphidius gifuensis to varying temperature conditions, as well as the stage-specific response of A. gifuensis to high temperatures. RESULTS: High temperatures had a significant impact on the both M. persicae and A. gifuensis. When exposed to 36°C, M. persicae developed more slowly and produced smaller adults compared to control, regardless of the duration of exposure (2, 4 or 6 h); additionally, the survival rate of M. persicae nymphs sharply decreased under these conditions. Exposure to 36°C for 4 h negatively impacted the development of A. gifuensis. Female parasitoids exposed to 32°C developed into smaller adults, whereas males exposed to all three temperature levels were smaller compared to control group. Female parasitoids exposed to high temperatures, regardless of the specific heat level and duration, exhibited reduced longevity and decreased fecundity. None of the parasitoids exposed to 36°C for 6 h daily developed into adults. Heat treated during early developmental stages (2 and 4 days old) had a greater influence on parasitoid development, whereas heat treatment at 4 and 6 days old had a more significant impact on its fecundity. CONCLUSION: High temperatures not only directly affected the performance of A. gifuensis, but also exerted indirect effects by influencing the quality of the host aphids M. persicae. The deleterious effects of high temperature on larvae can persist into the adult stage, affecting the longevity and reproduction of adults. These findings are important for the utilization of A. gifuensis in the control of M. persicae in warming environments. © 2024 Society of Chemical Industry.

Identification and Characterization of Antioxidant Enzyme Genes in Parasitoid Aphelinus asychis (Hymenoptera: Aphelinidae) and Expression Profiling Analysis under Temperature Stress.

It is well known that high temperature, a typically negative environmental factor, reduces the parasitism of a parasitoid. Generally, high temperature causes the rapid overproduction of reactive oxygen species (ROS) in organisms, and antioxidative enzymes participate in the process of resisting environmental stress by eliminating excess ROS. In the present study, we identify two superoxide dismutase (SOD), one catalase (CAT), six peroxidases (POD), and five glutathione-S-transferase (GST) genes; and the survival rate and antioxidative enzyme patterns under short-term high temperature exposure of the parasitoid wasp, A.asychis, are examined. Survival results of A.asychis reveal that females show higher thermal tolerance than males. Under short-term high-temperature exposure, in females, the expression levels of most antioxidant enzyme genes decreased first and then increased to a peak at 41 °C, while only the expression of AasyGST4 showed a continuous increase. In males, the expression patterns of most antioxidant enzyme genes fluctuated and reached a maximum at 41 °C. Moreover, the expression levels of the majority of antioxidant enzyme genes were higher in females than in males. In addition, at temperatures of and above 35 °C, the activities of these four antioxidant enzymes were induced. The results show that the antioxidant enzymes confer thermo-tolerance to A. asychis against lethal thermal stress. Our observations enrich the understanding of the response mechanism to high-temperature assaults of A. asychis.