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2.
NPJ Microgravity ; 9(1): 33, 2023 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-37120425

RESUMO

We conducted a charge-charge clustering experiment of positively and negatively charged colloidal particles in aqueous media under a microgravity environment at the International Space Station. A special setup was used to mix the colloid particles in microgravity and then these structures were immobilized in gel cured using ultraviolet (UV) light. The samples returned to the ground were observed by optical microscopy. The space sample of polystyrene particles with a specific gravity ρ (=1.05) close to the medium had an average association number of ~50% larger than the ground control and better structural symmetry. The effect of electrostatic interactions on the clustering was also confirmed for titania particles (ρ ~ 3), whose association structures were only possible in the microgravity environment without any sedimentation they generally suffer on the ground. This study suggests that even slight sedimentation and convection on the ground significantly affect the structure formation of colloids. Knowledge from this study will help us to develop a model which will be used to design photonic materials and better drugs.

3.
Acta Crystallogr D Struct Biol ; 77(Pt 5): 599-605, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-33950016

RESUMO

It is important to reveal the exact cause of poor diffractivity in protein crystals in order to determine the accurate structure of protein molecules. It is shown that there is a large amount of local strain in subgrains of glucose isomerase crystals even though the overall crystal quality is rather high, as shown by clear equal-thickness fringes in X-ray topography. Thus, a large stress is exerted on the subgrains of protein crystals, which could significantly lower the resistance of the crystals to radiation damage. It is also demonstrated that this local strain can be reduced through the introduction of dislocations in the crystal. This suggests that the introduction of dislocations in protein crystals can be effective in enhancing the crystal quality of subgrains of protein crystals. By exploiting this effect, the radiation damage in subgrains could be decreased, leading to the collection of X-ray diffraction data sets with high diffractivity.


Assuntos
Aldose-Cetose Isomerases/química , Difração de Raios X/métodos , Conformação Proteica
4.
Rev Sci Instrum ; 86(8): 083704, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26329200

RESUMO

The normal growth rates of the {110} faces of tetragonal hen egg-white lysozyme crystals, R, were measured as a function of the supersaturation σ parameter using a reflection type interferometer under µG at the International Space Station (NanoStep Project). Since water slightly evaporated from in situ observation cells during a long-term space station experiment for several months, equilibrium temperature T(e) changed, and the actual σ, however, significantly increased mainly due to the increase in salt concentration C(s). To correct σ, the actual C(s) and protein concentration C(p), which correctly represent the measured T(e) value in space, were first calculated. Second, a new solubility curve with the corrected C(s) was plotted. Finally, the revised σ was obtained from the new solubility curve. This correction method successfully revealed that the 2.8% water was evaporated from the solution, leading to 2.8% increase in the C(s) and C(p) of the solution.


Assuntos
Meio Ambiente Extraterreno , Muramidase/química , Astronave , Temperatura , Água/química , Cristalização , Solubilidade , Volatilização
5.
Rev Sci Instrum ; 84(10): 103707, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24182119

RESUMO

The growth rate vs. supersaturation of a lysozyme crystal was successfully measured in situ together with the crystal surface observation and the concentration measurements onboard the International Space Station. A Michelson-type interferometer and a Mach-Zehnder interferometer were, respectively, employed for real-time growth rate measurements and concentration field measurements. The hardware development, sample preparation, operation, and analysis methods are described.


Assuntos
Cristalização/instrumentação , Lasers , Muramidase/química , Ausência de Peso , Animais , Interferometria , Cinética , Temperatura
6.
Ann N Y Acad Sci ; 1027: 28-47, 2004 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-15644343

RESUMO

The National Space Development Agency of Japan (NASDA) developed a practical protocol to optimize protein crystallization conditions for microgravity experiments. This protocol focuses on the vapor diffusion method using high density protein crystal growth (HDPCG)--hardware developed by the University of Alabama, Birmingham--that flew on the STS-107 mission. The objective of this development was to increase the success rate of microgravity experiments by setting crystallization conditions based on knowledge of crystal growth and fluid dynamics. The protocol consists of four steps: (1) phase diagram preparation, (2) estimation of condensation rate in the vapor diffusion method, (3) fluid dynamic property measurement, and (4) fluid dynamic simulation. First, a phase diagram was constructed. Crystallization characteristics were investigated by a microbatch method. The data were recalculated based on classical nucleation theory and the crystallization boundary was determined as a function of time. The second step was to develop a practical model to estimate the condensation rate of the crystallizing solution, including protein and precipitant, as a function of the precipitant concentration and solution volume. By considering the crystallization map and the vapor diffusion condensation model we were able to optimize the crystallization conditions that generate crystals in the desired time. This was particularly important in a shuttle mission whose mission duration is limited. The third step was fluid dynamic property measurement necessary for fluid dynamics simulation and crystal growth study. The last step was to estimate the mass transport in space on the basis of the fluid dynamics simulation transport model. It turned out that neither the vapor phase nor the solution phase was seriously affected by gravity until nucleation provided the hardware was set in a normal direction. Therefore, we concluded that the optimized crystallization conditions could be directly applied to microgravity experiments. By completing the approach, we were able to control the time for nucleation in the vapor diffusion method.


Assuntos
Biofísica/métodos , Proteínas/química , Animais , Cristalização , Cristalografia , Difusão , Substâncias Macromoleculares , Muramidase/química , Voo Espacial , Astronave , Fatores de Tempo , Ausência de Peso
7.
Biotechnol Lett ; 25(4): 349-52, 2003 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-12882551

RESUMO

D-Fructose was isomerized to D-mannose using immobilized Agrobacterium radiobacter that produces a thermostable mannose isomerase. The cells were immobilized by adsorption on chitosan or by glutaraldehyde crosslinking in the presence of albumin. Optimum conditions for mannose isomerase activity were 60 degrees C and pH 7.5. Continuous reaction at 55 degrees C was achieved with immobilized cells packed in a column to produce D-mannose.


Assuntos
Aldose-Cetose Isomerases/metabolismo , Quitina/análogos & derivados , Frutose/metabolismo , Manose/biossíntese , Rhizobium/metabolismo , Albuminas , Células Imobilizadas/enzimologia , Células Imobilizadas/metabolismo , Quitosana , Ativação Enzimática , Estabilidade Enzimática , Enzimas Imobilizadas/metabolismo , Glutaral , Isomerismo , Controle de Qualidade , Rhizobium/classificação , Temperatura
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