Aniseikonia induced by cataract surgery and its effect on binocular vision.

PURPOSE: This study measured aniseikonia before and after the first and second cataract surgeries in ametropic adults. The relationship of aniseikonia to anisometropia and its effect on stereopsis, ocular alignment, and clinical symptoms were determined. METHODS: Seventeen patients scheduled to have bilateral cataract surgery with 2 diopters or more ametropia participated. Patients were evaluated before cataract surgery and 4 weeks (±1 week) after the first and second surgery. Visual acuity, refractive error, aniseikonia, stereopsis, ocular alignment, and visual symptoms were determined at each visit. RESULTS: Aniseikonia increased after the first cataract surgery. The increase in aniseikonia occurred in concert with increased anisometropia and resulted in poorer stereopsis overall. Aniseikonia and anisometropia 1 month (±1 week) after the second cataract surgery returned to near baseline and were associated with better stereopsis. The amount of aniseikonia showed substantial variance and could not be predicted by the amount of induced anisometropia. Changes in ocular alignment were minimal. Statistically significant changes in patient symptoms between study visits were infrequent. CONCLUSIONS: Aniseikonia induced by cataract surgery may not be a substantial problem for ametropic adults with normal binocular vision, at least in the short term.

Adaptation of impression cytology to enable conjunctival surface cell transcriptome analysis.

PURPOSE: This study investigates the extent of the human transcriptome that can be quantified from conjunctival impression cytology extracts. The aim is to determine if sufficient RNA can be isolated from a patient's conjunctival surface to identify differences in gene expression between dry eye and normal patients of (a) an array of 96 inflammatory biomarkers and associated receptors, and (b) if this comparison can be expanded to the entire transcriptome. MATERIALS AND METHODS: CIC was used to collect conjunctival surface cells from 53 qualifying normal and dry eye patients. Based on prior optimization of all assay steps, RNA was isolated from the samples using a Qiagen RNeasy Plus Mini Kit and qRT-PCR was used to determine gene expression of 96 genes using TaqMan Low Density Array cards. Samples from six normal and six dry eye patients were then assayed on an Illumina Human HT-12 BeadChip. RESULTS: Optimization steps yielded an RNA processing procedure that improved yield from an initial 12 genes through 96, then to the entire human transcriptome. For the HT-12 BeadChip, more than 30 genes differed by a factor of >1.5 between the dry eye and normal groups and seven genes were down-regulated by a factor of >2.0 in the dry eye group: HLA-DRB5, PSCA, FOS, lysozyme, TSC22D1, CAPN13 and CXCL6. CONCLUSIONS: Conjunctival impression cytology can be used to collect sufficient RNA from conjunctival surface cells that, when processed optimally, allows successful transcriptome-wide expression analysis. While the current transcriptome analysis used a limited patient group, larger studies of patients with various types and severities of dry eye should reveal significant gene expression trends that can then be targeted to improve dry eye treatment options.

Multiple cytokine analysis in human tears: an optimized procedure for cytometric bead-based assay.

PURPOSE: To compare instruments and reagents used for cytometric bead-based assay (CBA) of cytokines in human tears. METHODS: Two CBA instruments and reagent kits from five vendors were compared in parallel. Vendor protocols were modified to accommodate tear volumes. Precision, recovery, dilution linearity, and stability were evaluated. RESULTS: Luminextrade mark and Bio-Radtrade mark proved optimal for tear CBA. Good correlation for dilution linearity was observed (r > 0.80, p < 0.05) for 1-4-mu l sample volumes. Samples were stable for 30 min at 25 degrees C. CONCLUSION: Many tear cytokines can be reliably measured by CBA using an optimized protocol. Concentrations remain stable during periods consistent with collection.

The evaluation of two new computer-based tests for measurement of Aniseikonia.

PURPOSE: To evaluate the accuracy and repeatability of size lens induced aniseikonia measurement with the Aniseikonia Inspector Version 1 and a newer customized version of the Aniseikonia Inspector, Version 2. METHODS: Aniseikonia was measured on 27 subjects with both versions of the Aniseikonia Inspector in normal room illumination. Measurements of induced aniseikonia were made using size lenses in a randomized order. Twenty-five subjects were further tested in the dark using target sizes of equal visual angle for both tests. Repeatability of the intrinsic aniseikonia measurement was assessed on five subjects using randomized testing order for instrument and light and dark measurements. RESULTS: In normal illumination, the mean slopes for plots of induced aniseikonia vs. size lens magnification for Version 1 were 0.883 and 0.838 for the vertical and horizontal meridians, respectively. For Version 2, the corresponding slopes were 1.162 and 1.043. In the dark and using targets of the same size for both tests, the slopes for Version 1 were 1.038 in the vertical meridian and 0.866 in the horizontal meridian whereas for Version 2, the slopes were 1.195 in the vertical meridian and 1.127 in the horizontal meridian. The amount of underestimation or overestimation within any given testing condition showed considerable intersubject variation. Version 1 was more repeatable than 2, particularly in the vertical meridian. CONCLUSIONS: On average, the most accurate and repeatable measurement of aniseikonia was found with Version 1 in the vertical meridian in the dark. Measurement of aniseikonia in the horizontal meridian appears to be less reliable. Version 2 overestimates size lens-induced aniseikonia under all testing conditions. Intersubject variation in slopes of induced aniseikonia vs. size lens magnification should be further addressed.

Comparison of aniseikonia as measured by the aniseikonia inspector and the space eikonometer.

PURPOSE: The purpose of this study was to compare the new, computerized Aniseikonia Inspector with the Space Eikonometer for the measurement of aniseikonia. METHODS: Eighteen subjects, ages 21 to 61 years, with normal binocular vision and normal visual acuity had aniseikonia measured with both the Aniseikonia Inspector Version I and the Space Eikonometer. Aniseikonia was measured first with the subjects' habitual refractive correction and then with afocal size lenses of 1%, 2%, and 3.5% added in random order before the right and left eyes. Measurements were taken initially with the Aniseikonia Inspector and on a subsequent day with the Space Eikonometer. RESULTS: For the Space Eikonometer, the slopes of the lines for the relationship between the measured aniseikonia and the induced magnification differences in the vertical and horizontal meridians are not significantly different from 1.0. For the Aniseikonia Inspector, the slopes of the lines in the vertical and the horizontal meridians are less than and significantly different from 1.0. On average, the Aniseikonia Inspector underestimates the magnitude of induced aniseikonia, predicting only 68% and 61% of the overall size lens magnification in the vertical and horizontal meridians, respectively. The corresponding values of the Space Eikonometer in the vertical and horizontal meridians are 99% and 93%. Variability is greater with the Space Eikonometer than the Aniseikonia Inspector. CONCLUSION: The Space Eikonometer appears to be measuring the induced aniseikonia appropriately, whereas the Aniseikonia Inspector underestimates the amount of aniseikonia. However, the Space Eikonometer shows greater measurement variability. Modification of the Aniseikonia Inspector or the testing conditions should be pursued in future studies.