RESUMO
Clinical investigations and hematological, serum biochemical, and serological surveys were carried out on 11 male and 6 female Iriomote cats (Felis iriomotensis) in Japan. Examined Iriomote cats were considered clinically healthy by the inspection for the general physical conditions. However, urinalysis suggested the inflammation of the urinary tract in all the cats. Antibody for feline panleukopenia virus was positive in one of the examined Iriomote cats, which suggested a previous infection.
Assuntos
Carnívoros/sangue , Carnívoros/urina , Viroses/veterinária , Animais , Animais Selvagens , Anticorpos Antivirais/análise , Contagem de Células Sanguíneas/veterinária , Análise Química do Sangue/veterinária , Feminino , Japão/epidemiologia , Masculino , Valores de Referência , Estudos Soroepidemiológicos , Urinálise/veterinária , Viroses/epidemiologiaRESUMO
Electron microscopic observation was performed on platelets activated by collagen stimulation in Japanese Black cattle with delta-storage pool deficiency (delta-SPD) to identify their morphological and functional abnormalities compared from normal bovine platelets. Platelets of normal Japanese Black cattle changed their shapes to spherical and were in the phase of release reactions 5 min after the collagen (90.9 microg/ml) stimulation, and most of platelets were aggregated. On the other hand, in GSPD cattle, most of a granules were still dispersed in activated platelets, although the spherical shape change of the platelets was observed. These results suggested that there are abnormalities in the release reactions in platelets of delta-SPD cattle.
Assuntos
Plaquetas/efeitos dos fármacos , Doenças dos Bovinos/sangue , Colágeno/farmacologia , Agregação Plaquetária/efeitos dos fármacos , Deficiência do Pool Plaquetário/sangue , Animais , Plaquetas/ultraestrutura , BovinosRESUMO
Feline immunodeficiency virus (FIV) was isolated from a wild-caught Tsushima cat (Felis bengalensis euptilura), an endangered Japanese nondomestic subspecies of leopard cat (F. bengalensis). Phylogenetic analysis of the env gene sequences indicated that the FIV from the Tsushima cat belonged to a cluster of subtype D FIVs from domestic cats. FIVs from both the Tsushima cat and the domestic cat showed similar levels of replication and cytopathicity in lymphoid cell lines derived from these two species. The results indicated the occurrence of interspecies transmission of FIV from the domestic cat to the Tsushima cat in the wild.
Assuntos
Vírus da Imunodeficiência Felina/genética , Infecções por Lentivirus/virologia , Sequência de Aminoácidos , Animais , Animais Selvagens , Carnívoros , Gatos , Linhagem Celular , Efeito Citopatogênico Viral , Produtos do Gene env/genética , Vírus da Imunodeficiência Felina/classificação , Vírus da Imunodeficiência Felina/isolamento & purificação , Vírus da Imunodeficiência Felina/fisiologia , Dados de Sequência Molecular , Filogenia , Provírus/genética , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Replicação ViralRESUMO
Five Japanese Black cattle showing the tendency of persistent hemorrhage were diagnosed as delta-storage pool deficiency because of lack of dense bodies in platelets. There was no significant difference in the platelet count, fibrinogen concentration, prothrombin time and activated partial thromboplastin time between the cases and normal control cattle. However, the maximum platelet aggregation rate and the values of myosin in the cytoskeletons during platelet aggregation induced by collagen were significantly lower in the cases compared with those in normal control cattle. The quantities of platelet membrane glycoprotein were in the range of 94-160 kDa and were not different between the cases and control cattle. However, a decrease of thrombospondin in alpha-granules in platelet cytoplasm were suspected in two of the 5 cases.
