[Four years follow up in congenital talipes equino varus patients managed with Ponseti method]. / Seguimiento por cuatro años de pacientes con pie equinovaro aducto congénito manejados con método Ponseti.

INTRODUCTION: Congenital talipes equino varus (club foot) is a frequent congenital deformity of the foot. The Ponseti method is the gold standard for treatment. It consists of foot manipulation with weekly serial cast, minimally invasive surgery and Dennis-Brown bar up to five years. OBJECTIVE: To describe the follow-up of patients with PEVAC treated using the Ponseti method. MATERIAL AND METHODS: Descriptive, longitudinal study, during 2013-2019, in patients with PEVAC managed with Ponseti method. We included patients with uni- or bilateral club foot, under two years of age, without prior surgery, whose parents signed informed consent. Patients with other malformations were excluded. Serial weekly cast was placed for 4-8 weeks, a tenotomy of the Achilles tendon was performed, and cast for three more weeks; then reverse footwear with Dennis-Brown bar. The revisions were recorded at day zero, at eight weeks and every three months up to five years of age. Correction of deformity and pain on walking was assessed. RESULTS: There were 22 patients; 17 (77.3%) corrected more than 90% of the deformity, with adequate functionality and 86.3% without pain on gait, mean follow-up 3.9 years (1-7 years); six patients relapsed (27.27%) due to poor attachment, one re-treated with cast, and five with anterior tibial transfer, all successfully. CONCLUSIONS: The club foot managed with Ponseti method corrects more than 90% of the deformity and without or minimal pain with good adherence to treatment. We had a 27.27% recurrence in our series.

INTRODUCCIÓN: El pie equinovaro aducto congénito (PEVAC) es una deformidad congénita frecuente del pie. El método Ponseti es el estándar de oro para el tratamiento. Consiste en la manipulación del pie con yesos seriados semanales, una cirugía mínimamente invasiva y barra Dennis-Brown hasta los cinco años. OBJETIVO: Describir el seguimiento de los pacientes con PEVAC tratados mediante método Ponseti. MATERIAL Y MÉTODOS: Estudio descriptivo, longitudinal, durante 2013-2019, en pacientes con PEVAC manejados con método Ponseti. Se incluyeron pacientes con PEVAC uni- o bilateral, menores de dos años, sin cirugía previa, cuyos padres firmaron consentimiento informado. Se excluyeron pacientes con otras malformaciones. Se colocó yeso semanal seriado por cuatro a ocho semanas, se realizó tenotomía del tendón de Aquiles y yeso por tres semanas más; luego calzado de horma inversa con barra Dennis-Brown. Se registraron las revisiones al día cero, a las ocho semanas y cada tres meses hasta los cinco años de edad. Se valoró la corrección de la deformidad y el dolor a la marcha. RESULTADOS: Fueron 22 pacientes; 17 (77.3%) corrigieron más de 90% de la deformidad, con adecuada funcionalidad y 86.3% sin dolor a la marcha, seguimiento medio de 3.9 años (uno a siete años); seis pacientes tuvieron recidiva (27.27%) por mal apego, uno retratado con yesos y cinco con transferencia de tibial anterior, todos con éxito. CONCLUSIONES: El PEVAC manejado con método Ponseti corrige más de 90% de la deformidad y sin o mínimo dolor con buen apego al tratamiento. Tuvimos una recidiva de 27.27% en nuestra serie.

Experimental and theoretical vibrational study of methylene bis(thiocyanate), CH2(SCN)2. A comparison with thiocyanogen, (SCN)2.

The infrared and Raman spectra of methylene bis(thiocyanate), CH2(SCN)2, were obtained. The observed bands were assigned to the different normal modes of vibration using the results of a DFT calculation of the molecular vibrational properties. These results and the experimental data were used to define a Scaled Quantum Mechanics force field for the molecule. A similar treatment was applied to the thiocyanogen molecule, (SCN)2, for which the experimental frequencies were already reported in the literature. The sets of internal force constants for both molecules show very similar values.

Gastroallergic anisakiasis: findings in 22 patients.

BACKGROUND AND AIMS: Ingestion of Anisakidae larvae in raw seafood may cause anisakiasis. However, despite the high level of consumption of seafood in Spain, only a few cases of anisakiasis have been reported until now. Anisakis simplex can cause allergic reactions in sensitized patients as a result of its parasitism in the gastrointestinal tract. The purpose of this study was to analyse the clinical findings in 22 patients with gastroallergic anisakiasis. METHODS: Patients with allergic and/or gastric symptoms after seafood ingestion were evaluated in the emergency room of the La Paz General University Hospital. Skin testing for Anisakis simplex and tests on the implicated seafood were performed and amounts of serum-specific immunoglobulin E were assessed. A gastroscopy was performed in those patients with severe allergic or/and persistent gastric symptoms after ingestion of raw or undercooked seafood. RESULTS: Twenty-two patients were diagnosed with gastroallergic anisakiasis in 1 year. Most patients presented to the emergency room of our hospital with allergic symptoms. Gastric symptoms were usually moderate. Gastroscopy revealed local mucosal oedema and gastric erosion at the point of fixation. Two or more worms were detected in three patients. The mean time of latency of allergic symptoms was 5 h, while the mean time for gastric symptoms was 3 h. CONCLUSION: Anisakis simplex parasitism was the causative agent of allergic and gastric symptoms. Gastroallergic anisakiasis appears to be a relatively common disease, that may have been underdiagnosed.

[Indicators of oxidative stress and the effect of antioxidant treatment in patients with primary Parkinson disease]. / Indicadores de estrés oxidativo y efecto del tratamiento antioxidante en pacientes con enfermedad de Parkinson primaria.

INTRODUCTION AND OBJECTIVE: Parkinson's disease (PD) is characterized by a progressive, slow loss of dopaminergic neurones in the substance nigra. Although the cause of this neurone loss is unknown, at the present time many papers suggest oxidative stress (OS), secondary to dopaminergic metabolism, as an aetiopathogenic factor of PD. Therefore study of the part played by OS in this would permit the use of antioxidants (AO) as another possibility for treatment of the disease. It would also be a major step forward in the search for possible biological markers. MATERIAL AND METHODS: A study using spectrophotometric techniques was made of the serum levels of four biochemical indicators: catalase (CAT), malonyl aldehyde (MDA), phospholipase A2 (PLA2) and Vitamin C (VITC) in controls and in patients with PD. We found the average value for each of the variables studied in controls and in patients, taking AO treatment into account. RESULTS: The effect of clinical variables on serum levels of CAT, MDA, PLA2 and VITC was analyzed. It was seen that only the clinical state of Hoen and Yahr was related to the biochemical indicators. The CAT activity and VITC concentration showed statistically significant differences between patients (independently of their AO treatment) and controls. The CAT activity was significantly less in those treated with AO. The patients with PD did not all have the same degree of OS. The effect of AO treatment on plasma markers showed changes only in one subgroup of Parkinson patients. CONCLUSIONS: Our study suggests that AO treatment in this condition should be tailored to the individual patient according to the degree of OS present.

Indicadores de estrés oxidativo y efecto del tratamiento antioxidante en pacientes con enfermedad de Parkinson primaria / Indicators of oxidative stress and effect of the antioxidating treatment on patients with primary Parkinson disease

Introducción y objetivo. La enfermedad de Parkinson (EP) se caracteriza por una pérdida lenta y progresiva de las neuronas dopaminérgicas localizadas en la sustancia nigra. Aunque no se conoce la causa de la pérdida neuronal, en la actualidad es frecuente encontrar trabajos que proponen el estrés oxidativo (EO), secundario al metabolismo dopaminérgico, como factor etiopatogénico en la EP; por tanto, profundizar en el conocimiento del papel que desempeña el EO en ésta permitirá utilizar los antioxidantes (AO) como una alternativa más en el tratamiento de la enfermedad, además de constituir un paso importante en la búsqueda de posibles marcadores biológicos. Material y métodos. Se realizó un estudio de los niveles séricos de cuatro indicadores bioquímicos: catalasa (CAT), malonilaldehido (MDA), fosfolipasa A2 (PLA2) y vitamina C (VITC) en controles y pacientes con EP mediante técnicas espectrofotométricas. Se determinó la media para cada una de las variables estudiadas en controles y pacientes tomando en consideración el tratamiento AO. Resultados. Se analizó la influencia de las variables clínicas sobre los niveles séricos de la CAT, MDA, PLA2 y VITC y se observó que sólo el estadio clínico de Hoen y Yahr estaba relacionado con los indicadores bioquímicos. La actividad de la CAT y la concentración de VITC presentaron diferencias estadísticamente significativas entre pacientes (independientemente del tratamiento AO) y controles, siendo la actividad CAT significativamente menor en los tratados con AO. Todos los pacientes con EP no presentaron el mismo grado de EO; la influencia del tratamiento AO sobre los indicadores séricos sólo mostró diferencias en un subgrupo de parkinsonianos. Conclusiones. Nuestro estudio sugiere que el tratamiento AO en esta enfermedad debe ser individualizado y en concordancia con el grado de EO que presente el paciente

A method of dynamic analysis of iodine-123-metaiodobenzylguanidine scintigrams in cardiac mechanical overload hypertrophy and failure.

Cardiac sympathetic neuronal degeneration accompanies mechanical overload heart failure. We hypothesized that sympathetic nerve and myocyte failure share a common etiology and that 123I-metaiodobenzylguanidine (MIBG) might provide a precise method of detecting failure in chronic mechanical overload. Our aim was to develop a method for the dynamic analysis of 123I-MIBG scintigrams which could yield a quantitative index of myocardial sympathetic neuronal function in this condition. We performed serial 123I-MIBG scintigraphy in 33 volunteers, 10 orthotopic cardiac transplant recipients and 26 patients with chronic mechanical overload of the left ventricle. We constructed a compartmental model in which total heart activity represents the sum of cardiac sympathetic vesicular and cytosolic pools. Patients with antecedent mechanical overload heart failure or myocardial dysfunction had accelerated myocardial egress of tracer that we ascribed to a specific impairment in vesicular storage rather than to a more rapid turnover of an intact vesicular pool.