RESUMO
SBA-16 is a mesoporous nanostructure with high surface area and cubic symmetry which make them an ideal carrier to carry different nanoparticles. Zinc peroxide (ZnO2) is a novel nanoparticle that inhibits the growth of microorganisms due to its antiseptic property. Thin film of SBA-16 and SBA-16/ZnO2 was deposited on glass slide by spin coating method. Anti-biofilm property of the thin films was examined against E. coli and S. aureus bacteria. Structural property was found out by wide angle X-ray diffraction (XRD), small angle X-ray scattering (SAXS) and High Resolution Transmission Electron Microscope (HRTEM). Fourier Transform Infrared Spectrophotometer (FTIR) was used to confirmation the presence of ZnO2 in Nano-composite and AFM utilized for surface topography of thin films. Fluorescent microscope image clearly revealed that SBA-16/ZnO2 nanocomposite thin film significantly inhibit the biofilm formation against both E. coli (gram negative bacteria) as well as S. aureus (gram positive bacteria) in comparision to SBA-16. The antimicrobial behavior of ZnO nanoparticle is due to formation of H2O2 as reactive oxygen species (ROS) to which the bacterial cells are permeable. H2O2 is a powerful oxidizing agents that oxidized the mycobacterial elements like DNA and proteins which results into the toxicity of the bacteria cell.
Assuntos
Nanocompostos , Óxido de Zinco , Antibacterianos/farmacologia , Biofilmes , Escherichia coli , Peróxido de Hidrogênio/farmacologia , Espalhamento a Baixo Ângulo , Dióxido de Silício , Espectroscopia de Infravermelho com Transformada de Fourier , Staphylococcus aureus , Difração de Raios X , Óxido de Zinco/farmacologiaRESUMO
Bioactive glasses are inorganic biomaterials that have been used successfully in bone tissue engineering and in dentistry as filling materials. But due to poorer mechanical strength the bioactive glasses have limited application in load-bearing positions. If efforts are made to increase material strength, their bioactivity gets decrease. To comprehend the optimal toning between biological and mechanical properties, we aim to develop the new nano bioactive glass using simple sol-gel method having a composition of 50%SiO2-40%CaO-5%P2O5-2%ZnO-2%MgO-1%SrO (mol%) with three different bivalent metal ions (Zn2+, Mg2+ and Sr2+). The structural morphology, crystallinity, physical and chemical behavior of the prepared bioglass ceramics were analyzed through XRD, N2-adsorption measurements, FTIR, FESEM with EDS and HR-TEM. The bioactivity of the sample was analyzed in Dulbecco's modified eagle's medium solution for ten days. The synthesized nano bioglass-ceramic shows the apatite layer formation which was confirmed through XRD and FTIR and show anti-bacterial properties for the four well-known pathogens/microorganism Bacillus cereus, Candida albicans, Escherichia coli and Staphylococcus aureus.