Assessing marine litter in a highly polluted area in the Mediterranean: A multi-perspective approach in the Saronikos Gulf, Greece.

Marine litter poses a significant environmental challenge in the Saronikos Gulf, Greece. Employing an integrated approach, data from both beach and underwater sites were analyzed. The average litter density on beaches was 2.61 items m-2, with plastic being the most common material, notably small polystyrene fragments and cigarette butts. The western part of the Gulf exhibited higher litter density, mainly due to surface circulation patterns. Most beaches fell short of meeting Good Environmental Status criteria for marine litter. Higher litter densities were observed in autumn. Benthic litter density decreased with depth, being 23 times higher in shallower waters, with plastic being the predominant type. This extensive study offers crucial insights into the pollution status and litter distribution in the Saronikos Gulf, contributing significantly to the global understanding of marine litter distribution on coastal ecosystems. Such information is crucial for raising awareness, informing policy decisions, and driving environmental actions.

Paraffin waxes in the North-Western Mediterranean Sea: A comprehensive assessment in the Pelagos Sanctuary, a Specially Protected Area of Mediterranean Importance.

Paraffin waxes are widely recognized as emerging marine pollutants, even their classification by the recent monitoring programs and the knowledge of their occurrence, and sources of contamination in marine ecosystems are poorly defined and reported. Wax presence and distribution have been evaluated in different environmental compartments in the Pelagos Sanctuary (Mediterranean Sea) floating on the sea surface and stranded on beaches, focussing on their characterization, accumulation areas and pollution inputs. More than 2500 yellow paraffin residues were detected and analysed in the study area showing a prevailing dimension smaller than 5 mm. The Genoa Canyon and the waters facing Gorgona Island resulted in the more polluted areas representing two distinct hotspots of wax accumulation potentially related to the high density of tanker vessels sailing to and from the harbour of Genova and Livorno. Higher concentrations of beached particles were found along the Tuscan coast (11 items/100 m) and on Pianosa Island (110 items/m2). This study gives valuable insights into paraffin wax pollution in the Pelagos Sanctuary, emphasizing the need for harmonized monitoring and detection methods to elucidate the potential impacts on marine organisms. Moreover, mitigating actions are crucial to prevent and curb the waxes pollution of marine ecosystems.

Characterization of floating microplastic contamination in the bay of Marseille (French Mediterranean Sea) and its impact on zooplankton and mussels.

Microplastics (MPs) were sampled in three seasons from 2016 to 2018 in the Bay of Marseille, northwestern Mediterranean Sea, adjacent to a highly urbanized area. Six sites were selected according to their different characteristics (river mouth, treatment plants, protected marine area). Surface floating MPs were characterized (number, weight, typology and polymer) as was zooplankton. In addition, mussels were submerged and used to investigate ingestion. Finally, a hydrodynamic model was used to improve understanding of dispersion mechanisms. The annual averages of floating MPs values ranged from 39,217 to 514,817 items/km2. The MPs collected were mainly fragments principally composed of polyethylene and polypropylene. The mean abundance ratio (MPs/zooplankton) was 0.09. On average 87% of mussel pools were contaminated and ingested 18.73 items/100 g of flesh. Two hydrodynamic patterns were identified: the first retaining the MPs in the harbor, and the second dispersing them outside.

Lessons learned from an intercalibration exercise on the quantification and characterisation of microplastic particles in sediment and water samples.

An intercalibration exercise on the characterisation of microplastics in marine sediment and water samples was carried out among five laboratories involved in the implementation of the Marine Strategy Framework Directive (MSFD) in their country. The samples were prepared by mixing cleaned natural sediment and sea water with microplastics sets made of particles of various polymers, shapes and colours. Overall, the errors on total counts were under 25% in absolute value. The risk of non-detection and loss of particles is greater than the risk of contamination during sample analysis. Significant differences are observed among particle types. It appears difficult to obtain reliable and comparable data on the colour of microplastics. A comparison of the errors with regards to the protocols used led to recommend NaCl [1.2 g/cm3] density separation for sediment and one filtering step (200 µm). The operators' experience appears as a key factor for the quality of the results.

Seafloor litter from the continental shelf and canyons in French Mediterranean Water: Distribution, typologies and trends.

Seafloor litter has been studied both on the continental shelves (by trawling during 24 years) and in canyons (by ROV) of the French Mediterranean sea Water (FMW). On the continental shelf, mean densities range from 49.63 to 289.01 items/km2. The most abundant categories were plastic, glass/ceramics, metals and textiles. Trend analysis shows a significant increase in plastic quantities during the study period. Plastics accumulate at all depths, with heavier items being found in deeper areas, while the continental slope-break appears as a clean area. The spatial distribution of litter revealed the influence of geomorphologic factors, anthropic activities, shipping route, river inputs. All the canyons are affected by debris but coastal canyons (Ligurian Sea and Corsica) were more impacted than offshore canyons in the Gulf of Lion. The FMW appears to be highly polluted with regard to values found in other areas, but lower than those observed in the Eastern Mediterranean.

Tools and constraints in monitoring interactions between marine litter and megafauna: Insights from case studies around the world.

Adverse impacts of marine litter is documented on >1400 species, including marine megafauna (fish, birds, sea turtles and mammals). The primary impacts include ingestion and entanglement, and there is increasing concern about chemical contamination via ingestion. Numerous survey approaches and monitoring programs have been developed and implemented around the world. They may aim to provide data about parameters such as species distribution and interactions with anthropogenic activities. During the Sixth International Marine Debris Conference, a session was dedicated to the tools and constraints in monitoring interactions between litter and megafauna. In the present paper, we summarize 7 case studies which discuss entanglement and ingestion including macro- and micro-debris in several taxa and across multiple geographic regions. We then discusses the importance of tools and standardizing methods for assessment and management purposes, in the context of international environmental policies and marine litter strategies.

Optimising beached litter monitoring protocols through aerial imagery.

The monitoring of beached litter along the coast is an onerous obligation enshrined within a number of legislative frameworks (e.g. the MSFD) and which requires substantial human resources in the field. Through this study, we have optimised the protocol for the monitoring of the same litter along coastal stretches within an MPA in the Maltese Islands through aerial drones, with the aim of generating density maps for the beached litter, of assisting in the identification of the same litter and of mainstreaming this type of methodology within national and regional monitoring programmes for marine litter. Concurrent and concomitant in situ monitoring of beached litter enabled us to ground truth the aerial imagery results. Results were finally discussed within the context of current and future MSFD monitoring obligations, with considerations made on possible future policy implications.

The degradation potential of PET bottles in the marine environment: An ATR-FTIR based approach.

The dominance and persistence of plastic debris in the marine environment are well documented. No information exists in respect to their lifespan in the marine environment. Nevertheless, the degradation potential of plastic litter items remains a critical issue for marine litter research. In the present study, polyethylene terephthalate bottles (PETs) collected from the submarine environment were characterized using ATR-FTIR in respect to their degradation potential attributed to environmental conditions. A temporal indication was used as indicative to the years of presence of the PETs in the environment as debris. PETs seem to remain robust for approximately fifteen years. Afterwards, a significant decrease of the native functional groups was recorded; some even disappear; or new-not typical for PETs-are created. At a later stage, using the PET time series collected from the Saronikos Gulf (Aegean Sea-E. Mediterranean), it was possible to date bottles that were collected from the bottom of the Ionian Sea (W. Greece). It is the first time that such a study has been conducted with samples that were actually degraded in the marine environment.

A MSFD complementary approach for the assessment of pressures, knowledge and data gaps in Southern European Seas: The PERSEUS experience.

PERSEUS project aims to identify the most relevant pressures exerted on the ecosystems of the Southern European Seas (SES), highlighting knowledge and data gaps that endanger the achievement of SES Good Environmental Status (GES) as mandated by the Marine Strategy Framework Directive (MSFD). A complementary approach has been adopted, by a meta-analysis of existing literature on pressure/impact/knowledge gaps summarized in tables related to the MSFD descriptors, discriminating open waters from coastal areas. A comparative assessment of the Initial Assessments (IAs) for five SES countries has been also independently performed. The comparison between meta-analysis results and IAs shows similarities for coastal areas only. Major knowledge gaps have been detected for the biodiversity, marine food web, marine litter and underwater noise descriptors. The meta-analysis also allowed the identification of additional research themes targeting research topics that are requested to the achievement of GES.

A comparative study of marine litter on the seafloor of coastal areas in the Eastern Mediterranean and Black Seas.

In the present work, abundance, spatial distribution and qualitative composition, of benthic marine litter, were investigated in five study areas from the Eastern Mediterranean and Black Seas (Saronikos, Patras and Echinades Gulfs; Limassol Gulf; Constanta Bay). Surveys were performed using the monitoring protocol proposed by the Technical Group for Marine Litter. Densities ranged from 24items/km(2) to 1211items/km(2), with the Saronikos Gulf being the most affected area. Plastics were predominant in all study areas ranging from 45.2% to 95%. Metals and Glass/Ceramics reached maximum values of 21.9% and of 22.4%. The size distribution of litter items showed that ⩾50% fall into medium size categories (10×10cm, 20×20cm) along with an elevated percentage of small-sized (<5×5cm) plastic litter items. The comparative analysis of the data highlighted the dependence of the marine litter problem on many local factors (human sources and oceanographic conditions) and the urgent need for specific actions.

Monitoring the impact of litter in large vertebrates in the Mediterranean Sea within the European Marine Strategy Framework Directive (MSFD): constraints, specificities and recommendations.

In its decision (2010/477/EU) relating to the European Marine Strategy Framework Directive (MSFD, 2008/56/EC), the European Commission identified the following points as focuses for monitoring: (i) 10.1.1: Trends in the amount, source and composition of litter washed ashore and/or deposited on coastlines, (ii) 10.1.2: Trends in the amount and composition of litter in the water column and accumulation on the sea floor, (iii) 10.1.3: Trends in the amount, distribution and composition of micro-particles (mainly microplastics), and (iv) 10.2.1: Trends in the amount and composition of litter ingested by marine animals. Monitoring the impacts of litter will be considered further in 2014. At that time, the strategy will be discussed in the context of the Mediterranean Sea, providing information on constraints, protocols, existing harm and research needed to support monitoring efforts. The definition of targets and acceptable levels of harm must take all factors into account, whether entanglement, ingestion, the transport and release of pollutants, the transport of alien species and socio-economic impacts. It must also reflect on the practical deployment of "ingestion" measures (10.2.1). The analysis of existing data will reveal the potential and suitability of some higher trophic level organisms (fish, turtles, birds and mammals) for monitoring the adverse effects of litter. Sea turtles appear to be useful indicator species, but the definition of an ecological quality objective is still needed, as well as research on alternative potential indicator species.

Plastic litter in the sea.

On June 2013 a workshop at the University of Siena (Italy) was organized to review current knowledge and to clarify what is known, and what remains to be investigated, concerning plastic litter in the sea. The content of the workshop was designed to contribute further to the European Marine Strategy Framework Directive (MSFD) following an inaugural workshop in 2012. Here we report a number of statements relevant to policymakers and scientists that was overwhelming agreement from the participants. Many might view this as already providing sufficient grounds for policy action. At the very least, this early warning of the problems that lie ahead should be taken seriously, and serve as a stimulus for further research.

Efecto de la ingesta aguda de vanillina sobre la resistencia insulínica en humanos / Effect of a single oral dose of vanillin on insulin resistance in humans

Background: NADPH oxidase is a source of reactive oxygen species that may contribute to insulin resistance (IR). Aim: To assess the effect of a single oral dose of vanillin (a putative inhibitor of the enzyme) on IR in humans. Material and Methods: Using a crossover, random, double-blind design, eight lean and 10 obese males ingested 600 mg of vanillin or placebo followed by the ingestion of 75g of glucose. Serum/plasma glucose, free-fatty acids, insulin, glutathione, C reactive protein concentrations and red blood cell glutathione concentration were determined. Insulin resistance was estimated by the Matsuda index. Results: Under fasting conditions, obese individuals had higher glucose and insulin and lower red blood cell glutathione levels than their lean counterparts (p < 0.01). Serum free-fatty acids, total and oxidized plasma glutathione concentrations were similar in both groups. After glucose ingestion, obese individuals had a lower red blood cell total glutathione concentration and increased plasma oxidized glutathione concentration than their lean counterparts (p < 0.05). In addition, obese participants had a higher level of IR (p < 0.001) and impaired serum free-fatty acid suppression (p < 0.001) than their lean counterparts. Ingestion of vanillin did not modify any of these variables when compared with placebo in obese individuals. In lean volunteers a reduction in Matsuda index was detected when vanillin was administered, compared to placebo (4.3 +/- 0.6 and 3.6 +/- 0.6 respectively; p < 0.05). Conclusions: IR was ameliorated after vanillin ingestion among lean but not obese participants.

Etiopatogenia de la obesidad / Etiopathogenesis of obesity

La investigación de los factores etiopatogénicos de la obesidad, sean estos ambientales o genéticos, ha influido fuertemente en el desarrollo de las estrategias para controlar la enfermedad. Se considera a la obesidad como una enfermedad en la cual confluyen factores genéticos y ambientales, pero no se han dilucidado cabalmente los mecanismos de regulación del balance energético, cuyas alteraciones favorecen el incremento de la adiposidad en humanos. Entre los factores ambientales, además de la alimentación alta en calorías y el sedentarismo, se describen factores predisponentes sociales, culturales y familiares, así como situaciones o etapas fisiológicas de la vida que pueden gatillar aumento de peso. Aunque los factores y patologías genéticas se pueden evidenciar en un bajo porcentaje de los sujetos obesos, la predisposición genética a la obesidad se puede expresar a través de algunas características metabólicas, como un bajo gasto energético de reposo, mala oxidación de grasas o un bajo nivel de actividad física espontánea.

The investigation of the etiological factors of obesity, whether genetic or environmental, has strongly influenced the development of strategies to control the disease. Obesity must be considered as a disease in which genetic and environmental factors converge. However, the mechanisms of regulation of the energy balance, which changes favoring increased adiposity in humans, are not fully elucidated. Among environmental factors, in addition to high-calorie diet and sedentary lifestyle, it has been described social, cultural and family predisposing factors, as well as physiological conditions or stages of life that can trigger weight gain. Although genetic factors and pathologies can be evidenced in a low percentage of obese subjects, genetic predisposition to obesity can be expressed through some metabolic characteristics such as low metabolic rate, poor oxidation of fats or a low level of spontaneous physical activity.

Minerals as additives for decreasing the toxicity of Mediterranean contaminated dredged sediments.

The management of dredged sediments is a priority issue in the Mediterranean sea where sediments are historically polluted. The aims of this study were to evaluate the toxicity of port sediment samples and the effect of three mineral additives (hematite, zerovalent iron (ZVI) and natural zeolite (NZ)) on sediment elutriate toxicity. Four sediments (A, B, C and D) were provided by port authorities after composting procedure; particle size, particulate organic carbon, metals and organic pollutants (TBT, PAHs, PCBs) were determined in whole sediments. Elutriates from these composted sediments were analyzed by determining toxicity level using oyster (Crassostrea gigas) larvae bioassay, metal and dissolved organic carbon concentrations. Toxicity, measured on undiluted elutriates (250 g/L), decreased as follows: A≥B>C∼D. The treatment of sediments with mineral additives (5%) revealed that hematite tends to decrease the elutriate toxicity in all samples, particularly in samples B and C. This effect may be related to metal concentration decrease in elutriates, in particular Cu and Zn, that have a significant toxic effect on oyster larvae. ZVI and NZ have a variable influence on elutriate toxicity. Results suggest that hematite may be a possible candidate for decreasing chemical concentration and improving the quality of elutriates. Hematite could be used for sediment stabilization prior to the deposit in a specific site or landfill.

Multiple biomarkers of pollution effects in caged mussels on the Greek coastline.

A suite of biomarkers was measured in caged mussels at areas impacted by different anthropogenic activities along the Greek coastline to assess biological effects of environmental pollution. Mussels were caged at coastal sites in the vicinity of major cities, in areas influenced by major industries, agricultural practices and in islands away from known sources of pollution. Biomarkers indicative of neurotoxicity (acetylcholinesterase, AchE), oxidative stress (catalase, CAT), phase II biotransformation of xenobiotics (glutathione S-transferase, GST), metal exposure (metallothioneins, MTs) and protein synthesis (RNA:DNA ratio) were measured to assess effects of various types of pollutants. AchE activity proved to be the most responsive biomarker with decreased values at sites influenced by agricultural, urban and industrial activities. Decreased CAT and GST activities and increased MTs levels were recorded at a number of anthropogenic-impacted sites. RNA:DNA ratio showed a biphasic response as both high and low values were found at impacted sites. Principal component analysis clearly distinguished sites receiving pollution inputs from non-polluted sites. The combination of the selected biomarkers used in caged mussels resulted useful in the assessment of the effects of environmental pollution.

Assessment of the environmental quality of French continental Mediterranean lagoons with oyster embryo bioassay.

In order to better understand environmental disturbances in the French coastal Mediterranean lagoons, we used an ecotoxicological approach based on the measurement of the toxicity of the sediments using oyster embryo bioassay that provides a basis for assessing the effects on the fauna of contaminants adsorbed on the sedimentary particles. The study covers all of the main lagoons of the French Mediterranean coasts of Languedoc Roussillon, Camargue, and Provence (Berre and Bolmon lagoons), where 188 stations were sampled. The toxicity tests provide evidence of variable levels of toxicity in sediments. Contaminated lagoons such as La peyrade, Le canet, and Ingrill and locally affected lagoons such as Bages-Sigean, Vaccares, Bolmon, and Berre have sampling stations with 100% of larval abnormalities during 24-h development. In all of the lagoons, the toxicity was mainly located close to local harbors and rivers. Salses Leucate (Languedoc roussillon) lagoon was found very clean, with no important toxicity. The results are discussed in terms of environmental disturbances of the coastal lagoons and with regard to the long-term monitoring of the impact of contaminants on the coastal environment.

Assessment of environmental pollution at Balearic Islands applying oxidative stress biomarkers in the mussel Mytilus galloprovincialis.

The antioxidant enzyme response of the mussel Mytilus galloprovincialis to different degree of pollution was investigated. Antioxidant enzyme activities - catalase (CAT), glutathione peroxidase (GSH-PX), glutathione reductase (GR), superoxide dismutase (SOD) - and malondialdehyde (MDA) concentration were measured in gills and digestive glands of mussels. Mussels from the same origin were transplanted along the Balearic coastal waters in eight stations characterized by a different degree of contamination and human impacts. Antioxidant enzyme activities showed an adaptive response to increase the activities in the more polluted areas. CAT, GR and SOD in gills and CAT and GR in digestive gland presented significant differences between polluted and non-polluted stations. No significant differences were observed in MDA concentration indicating that the antioxidant response is capable to avoid the lipid peroxidation. The use of biomarkers such as CAT and GR in gills and digestive glands of the mussel M. galloprovincialis is a good tool to categorize differences between polluted and non-polluted areas.

Determinación del índice glicémico del alimento NutriDiabetic® destinado a diabéticos tipo 2 / Determination of glycemic index of NutriDiabetic®, a food targeted to type-2 diabetic subjects

La diabetes tipo 2 es un desorden del metabolismo de sustratos donde la dieta juega un papel crítico en mantener niveles de glicemia estables. La cantidad y tipo de carbohidratos son los principales determinantes de la glicemia. El índice glicémico es reconocido como un parámetro para determinar el impacto agudo de los carbohidratos de un alimento en la glicemia. Así, alimentos de alto índice glicémico promueven un aumento de la glicemia, y lo opuesto sucede con los de bajo índice glicémico. Este estudio evaluó el índice glicémico de un alimento (NutriDiabetic®) para diabéticos en 10 sujetos controles y 9 diabéticos. En ambos grupos, la ingestión de 50g de carbohidratos disponibles a partir de NutriDiabetic®, indujo una menor respuesta glicémica comparado con 50g de glucosa. Como consecuencia, el índice glicémico del alimento fue 27 por ciento y 54 por ciento para controles y diabéticos, respectivamente. La respuesta glicémica estimada para pan blanco será 85 por ciento mayor a una cantidad igual de carbohidratos a partir de NutriDiabetic®. En el manejo dietético de diabéticos tipo 2 es necesario considerar a la carga glicémica como un índice más apropiado para estimar la respuesta glicémica, dado que combina el índice glicémico con la cantidad de carbohidratos del alimento.

Type-2 diabetes is a fuel metabolism disorder with the diet being critical to maintain normal glycemic levels. The amount and type of carbohydrate are the main determinant factors of glycemia. The glycemic index is a known parameter to estimate the acute blood glucose impact of carbohydrate foods. Thus, high glycemic index foods promote increased glycemia, and the opposite occurs for low glycemic index foods. This study evaluated the glycemic index of a specially-designed diabetic food (NutriDiabetic®) in 10 non-diabetic and 9 diabetic subjects from both sexes. In both groups, the ingestion of 50g of available carbohydrates from NutriDiabetic® induced a lower postprandial glycemic response in comparison to the administration of 50g of glucose. As consequence, the glycemic index from this new food was 27 percent for non-diabetic and 54 percent for diabetic subjects. The estimated blood glucose reponse to white bread would be 85 percent higher to equal amounts of available carbohydrates from NutriDiabetic®. For the dietetic management of type-2 diabetic patients, it is necessary to consider the glycemic load as a more adequate index to estimate blood glucose response.

Evaluación de la situación de ácidos grasos esenciales y derivados de cadena larga en la dieta de lactantes menores de un año en Chile / Assessment of the status for essential fatty acids and long-chain derivatives in the diet of children younger than 1 year old from Chile

Los ácidos grasos esenciales (AGE) son precursores de ácidos grasos de muy larga cadena. Se concentran en el tejido nervioso, habiéndose sugerido que tienen un rol crítico en el desarrollo cerebral y visual. Se desconoce si la conversión de AGE a estos ácidos grasos es suficiente para sustentar una función óptima, por lo tanto es prioritario definir los requerimientos de AGE y sus derivados. Algunos autores sugieren incorporar estos ácidos grasos derivados en las fórmulas lácteas. Este artículo revisa y evalúa la ingesta aparente de ácidos grasos esenciales y derivados a partir de la fórmula láctea consumida por los lactantes beneficiarios del Programa Nacional de Alimentación Complementaria durante el primer año de vida, comparándola con las recomendaciones de lípidos recientemente propuestas. Se observó una ingesta deficitaria de ácido docosahexaenoico en lactantes alimentados con fórmulas lácteas no modificadas o sin adición de derivados de cadena larga.