Environmental methicillin-resistant Staphylococcus aureus contamination in pig herds in relation to the productive phase and application of cleaning and disinfection.

The aim of the study is to better understand MRSA environmental contamination in pig holdings in relation to the productive phase and the application of environmental cleaning and disinfection practices (C&D). This study was carried out on six herds. Dust samples from farrowing crates, weaning boxes, growing and finishing pens were collected from each herd environment prior to and after C&D and cultured for MRSA. Ten samples were collected at the end of each productive phase with the animals present and 10 additional samples were collected after C&D, prior to introduction of a new stock. The proportion of positive samples prior to C&D was lower in fattening than in other phases. The proportion of positive samples prior to and after C&D was 121/240 (50%) and 46/240 (19%) respectively. In the mixed effects logistic regression analysis it was shown not only that both productive phase and C&D were associated with the probability of having a positive sample but also that the C&D effect was different in the different productive phases. In particular the effect of C&D was stronger in farrowing crates than in the other productive phases. The results of this study show that, although current practices of cleaning and disinfection reduce MRSA environmental contamination, they are likely to be inadequate to the elimination of the microorganism. However, a strict application of hygienic protocols can lead to a marked reduction of MRSA environmental contamination.

Transverse-mode selection in apertured super-Gaussian resonators: an experimental and numerical investigation for a pulsed CO(2) Doppler lidar transmitter.

A representative prototype of a high-energy, long-pulse, and narrow-bandwidth pulsed CO(2) laser suitable for a spaceborne Doppler wind lidar application has been developed. We obtained 10 J of output energy at greater than 8% efficiency in long, narrow-bandwidth, single-longitudinal, and transverse-mode pulses. We used a positive branch unstable resonator with a fourth-order super-Gaussian mirror as the output coupler. Experiments were carried out to assess the effect of intracavity hard apertures of different diameters that induce diffractive perturbation of the theoretical field and reduce the transverse-mode selectivity of the cavity. An upper limit to the choice of the mirror soft radius has been found, which allows optimization of the trade-off between laser efficiency and beam quality. We determined experimentally that a value of 0.75-0.8 for the ratio between the exp(-1) diameter of the beam intensity and the laser clear aperture gave a single-transverse-mode operation without significant loss of efficiency.

[Peroxidation of erythrocyte lipids and levels of vitamin E in red blood cells in children with chronic renal insufficiency]. / Perossidazione dei lipidi eritrocitari e livelli di vitamina E nei globuli rossi dei bambini con insufficienza renale cronica.

The tocopherol and malonyldialdeyde levels in the erythrocytes of 15 children affected by chronic renal failure, free of therapy, have been studied. Decreased levels of the former and increased of the latter were found. These data indicate red blood cell lipids peroxidation, responsible, at least in part, of the reduced red blood cell survival and of chronic anemia.

[Clinical study of a new preparation with antiseptic and antiphlogistic action on the female genital tract]. / Studio clinico su di un nuovo preparato ad azione antisettica ed antiflogistica sulle vie genitali femminili

PIP: 55 patients aged 20-68 with various forms of genital tract infection or diseases, such as vaginitis or cervicitis, were treated for 12 days with Agena, an antiseptic and antiinflammatory preparation containing benzalkonium chloride and nonoxynol-9. Disappearance or regression of symptoms was noted in all cases, and no side effects were reported. Gynecological examination and bacteriological tests showed evidence of the efficacy and tolerability of the product.^ieng