[THE CLINICAL COURSE OF HEPATITIS C IN CHILDREN WITH DIFFERENT VARIANTS OF THE GENE POLYMORPHISM IL28B].

Aim of investigation: The aim of the research is to study the clinical course of hepatitis C in children with different variants of the gene polymorphism of IL-28B. Materials and methods: We observed 94 children (46 girls and 48 boys) with chronic hepatitis C (CHC) in age from 3 to 17 years (mean age 10 years). There were significant differences in the distribution of allele frequencies in children with chronic hepatitis C and in the population. In children with chronic hepatitis C significantly increased the incidence of the T allele at the locus of the gene IL-28B rs12979860 C>T, which makes it possible to consider it as a predictor of antiviral therapy ineffective. Results: When analyzing the frequency of occurrence of a polymorphic variant T>G [rs8099917] IL-28B gene in children with chronic hepatitis C and healthy children revealed no differences in the distribution of alleles. Conclusion: Personalized approach to the appointment of HCV antiviral therapy in children is to carry out genetic studies to determine on the basis of predictive features of the course of HCV in children during the treatment.

[Modern trends of research in nutritiology and nutrition hygiene].

Advances in instrumental analysis and new knowledge in biology and medicine have allowed nutritiology and nutrition hygiene: 1) to go from studies of dietary intake of the population and the establishment of standards nutrient and energy needs to ensuring of public health and prevention of diseases by changing the composition and structure of nutrition; 2) to assume that the nutrition includes all the processes of cell and organism vital functions; 3) to develop an individualized nutrition and dietetics purposeful; 4) to evaluate energy value of ontogeny; 5) to ensure food safety at all stages of its manufacture; 6) to combine many disciplines into a single unit to achieve scientific and practical problems. Nutritiology achievements of the 21st century will be based on the development of cell biology as a basis for systematic studies of ontogenetic development of a unicellular or multicellular organism on the external factors, including food. OMICs-disciplines should be used for these purposes in order to understand the physiological meaning of transmission and coding signals to food interaction of genes, proteins, metabolites inside cells or in the organism, to reveal the mechanisms of encoding cell responses to these specific interactions. The nutrition process covers all aspects of life of the cell and organism. Postnatal ontogenetic development of placental mammals has its own specific energy dimension. Consumption of 100 kJ/kg body weight provides 1/50,000 of their ontogeny. A hypothesis of lifespan regulating by changing the rate of ontogenetic development (aging) of organs and tissues of mammals, depending on the amount of energy consumed and food has been offered. Heterochronicity of internal organs and tissues aging depends on the work they are doing and the specific impact on them of food and substances. Research should be directed at identifying the regulatory properties of food substances on pre- and postnatal ontogenetic development of human. This knowledge will serve as a basis for the development of care to ensure the health of the population.

Dynamics of locomotor activity and heat production in rats after acute stress.

The dynamics of locomotor activity and heat production were studied in rats demonstrating passive and active behavior in the open field test at different time after exposure to acute emotional stress caused by 12-h immobilization during dark hours. The most pronounced changes in behavior and heat production followed by disturbances in circadian rhythms of these parameters were detected within the first 2 days after stress. In contrast to behaviorally active rats, the most significant decrease in locomotor activity and heat production of passive animals subjected to emotional stress was observed during dark hours. Circadian rhythms of behavior and heat production in rats tended to recover on day 3 after immobilization stress. These data illustrate the specificity of metabolic and behavioral changes reflecting the shift of endogenous biological rhythms in individuals with different prognostic resistance to stress at different terms after exposure to negative emotiogenic stimuli.

Proteomic study of rat hippocampus under conditions of emotional stress.

Proteomic differences in the hippocampus of stress-resistant and stress-sensitive rats were detected on the model of emotional stress. Differential expression of some proteins was detected in animals with different behavioral activity initially and after experimental stress exposure. Specific involvement of the hippocampus in the realization of stress response in animals with different sensitivity to emotional stress was demonstrated.

Effect of coenzyme Q10 on the proteomic profile of the cytosolic and microsomal fractions from rat hepatocytes upon dietary consumption of various lipid components during ontogeny.

We studied proteomic features of subcellular fractions from rat hepatocytes and intensity of enzymatic and non-enzymatic free radical oxidation depending on the type of dietary fat during adaptation of animals to modified nutrition. Our results illustrate the formation of specific nutriproteomes in the microsomal and cytosolic fractions of rat hepatocytes (measurement of the macro-component and micro-component composition of diet).

Peculiarities of rat serum proteome profile in metabolic stress.

We identified individual proteins characterizing starvation-induced metabolic stress. Sustained changes at the level of proteome caused by metabolic stress were demonstrated. Intensified synthesis of S6 kinase ribosomal protein, immunoglobulin lambda light chains, and inter-alpha-trypsin inhibitor was observed in the experimental groups.

Effect of taurine on the proteomic profile of the cytosolic and microsomal fractions of rat hepatocytes during ontogeny.

The proteomic features of the cytosolic and microsomal fractions of rat hepatocytes were studied during long-term dietary consumption of taurine (12 months) as a modulator of energy homeostasis. We identified proteomic markers of the effect of taurine on regulation of cell homeostasis. A protein with unknown biological function was revealed.

Effect of coenzyme Q10 on proteomic profile of blood plasma and cytosolic and microsomal fractions of rat hepatocytes during ontogeny.

The proteomic features of blood plasma and subcellular fractions of rat hepatocytes were studied during long-term dietary consumption of coenzyme Q10 as the endogenous mediator of antioxidant and energy homeostasis in the cell. Long-term coenzyme Q10 consumption was followed by the formation of specific nutriproteomes of the microsomal and cytosolic fractions of rat hepatocytes.

Effect of coenzyme Q10 on proteomic profile of rat plasma under conditions of metabolic stress.

Proteomic profile of rat plasma was evaluated under conditions of metabolic stress (5-day starvation) and administration of coenzyme Q10 in doses of 10 and 100 mg/kg. This antioxidant and geroprotector plays a key role in cell metabolism. The expression of some low-molecular-weight proteins in response to treatment with coenzyme Q10 was observed.

Study of biological activity of Lys-Glu-Asp-Trp-NH2 endogenous tetrapeptide.

Biological effect of natural tetrapeptide Lys-Glu-Asp-Trp-NH(2)was evaluated during the ontogeny and in experimental streptozotocin-induced diabetes mellitus (a model of rapid experimental aging) by the sum of metabolic parameters characterizing apoptosis.

[Medical and biological safety assessment of genetically modified maize strain MIR604].

The results of toxicologo-hygienic examinations, which were conducted within the framework of integrated medical and biological assessment of genetically modified rootworm Diabrotica spp.-protected maize event MIR604, are presented. Analysis of morphological, hematological, biochemical parameters and system (sensitive) biomarkers has not confirmed any toxic effect of maize event MIR604.