Comparison of Printable Biomaterials for Use in Neural Tissue Engineering: An In Vitro Characterization and In Vivo Biocompatibility Assessment.

Nervous system traumatic injuries are prevalent in our society, with a significant socioeconomic impact. Due to the highly complex structure of the neural tissue, the treatment of these injuries is still a challenge. Recently, 3D printing has emerged as a promising alternative for producing biomimetic scaffolds, which can lead to the restoration of neural tissue function. The objective of this work was to compare different biomaterials for generating 3D-printed scaffolds for use in neural tissue engineering. For this purpose, four thermoplastic biomaterials, ((polylactic acid) (PLA), polycaprolactone (PCL), Filaflex (FF) (assessed here for the first time for biomedical purposes), and Flexdym (FD)) and gelatin methacrylate (GelMA) hydrogel were subjected to printability and mechanical tests, in vitro cell-biomaterial interaction analyses, and in vivo biocompatibility assessment. The thermoplastics showed superior printing results in terms of resolution and shape fidelity, whereas FD and GelMA revealed great viscoelastic properties. GelMA demonstrated a greater cell viability index after 7 days of in vitro cell culture. Moreover, all groups displayed connective tissue encapsulation, with some inflammatory cells around the scaffolds after 10 days of in vivo implantation. Future studies will determine the usefulness and in vivo therapeutic efficacy of novel neural substitutes based on the use of these 3D-printed scaffolds.

A Novel In Vitro Pathological Model for Studying Neural Invasion in Non-Melanoma Skin Cancer.

Neural Invasion (NI) is a key pathological feature of cancer in the colonization of distant tissues, and its underlying biological mechanisms are still scarcely known. The complex interactions between nerve and tumor cells, along with the stroma, make it difficult to reproduce this pathology in effective study models, which in turn has limited the understanding of NI pathogenesis. In this study, we have designed a three-dimensional model of NI squamous cell carcinoma combining human epidermoid carcinoma cells (hECCs) with a complete peripheral nerve segment encapsulated in a fibrine-agarose hydrogel. We recreated two vital processes of NI: a pre-invasive NI model in which hECCs were seeded on the top of the nerve-enriched stroma, and an invasive NI model in which cancer cells were immersed with the nerve in the hydrogel. Histological, histochemical and immunohistochemical analyses were performed to validate the model. Results showed that the integration of fibrin-agarose advanced hydrogel with a complete nerve structure and hECCs successfully generated an environment in which tumor cells and nerve components coexisted. Moreover, this model correctly preserved components of the neural extracellular matrix as well as allowing the proliferation and migration of cells embedded in hydrogel. All these results suggest the suitability of the model for the study of the mechanisms underlaying NI.

Genipin crosslinking promotes biomechanical reinforcement and pro-regenerative macrophage polarization in bioartificial tubular substitutes.

Traumatic nerve injuries are nowadays a significant clinical challenge and new substitutes with adequate biological and mechanical properties are in need. In this context, fibrin-agarose hydrogels (FA) have shown the possibility to generate tubular scaffolds with promising results for nerve repair. However, to be clinically viable, these scaffolds need to possess enhanced mechanical properties. In this line, genipin (GP) crosslinking has demonstrated to improve biomechanical properties with good biological properties compared to other crosslinkers. In this study, we evaluated the impact of different GP concentrations (0.05, 0.1 and 0.2% (m/v)) and reaction times (6, 12, 24, 72 h) on bioartificial nerve substitutes (BNS) consisting of nanostructured FA scaffolds. First, crosslinked BNS were studied histologically, ultrastructurally and biomechanically and then, its biocompatibility and immunomodulatory effects were ex vivo assessed with a macrophage cell line. Results showed that GP was able to improve the biomechanical resistance of BNS, which were dependent on both the GP treatment time and concentration without altering the structure. Moreover, biocompatibility analyses on macrophages confirmed high cell viability and a minimal reduction of their metabolic activity by WST-1. In addition, GP-crosslinked BNS effectively directed macrophage polarization from a pro-inflammatory (M1) towards a pro-regenerative (M2) phenotype, which was in line with the cytokines release profile. In conclusion, this study considers time and dose-dependent effects of GP in FA substitutes which exhibited increased biomechanical properties while reducing immunogenicity and promoting pro-regenerative macrophage shift. These tubular substitutes could be useful for nerve application or even other tissue engineering applications such as urethra.

Muscle contraction time after caffeine intake is faster after 30 minutes than after 60 minutes.

BACKGROUND: This study aimed to determine the optimal time point, either 30 or 60 minutes, at which muscle reactivity to caffeine administration is highest. Unlike previous studies that focused on the nervous system response, we employed tensiomyography (TMG) to directly assess the effects of caffeine on muscle fibers. METHODS: TMG measurements were performed on the gastrocnemius medialis muscle of 42 male athletes who regularly consumed caffeine. Participants received a dose of 6 mg/kg body weight and TMG measurements were taken prior to caffeine intake, as well as 30 and 60 minutes afterward. RESULTS: Analysis of TMG parameters including time to contraction (Tc), time delay (Td), and maximal displacement (Dm) revealed that muscles exhibited faster contractions and greater stiffness at the 30-minute mark compared to both pre-caffeine intake and the 60-minute time point. Time exerted a significant main effect on Tc (F(2, 246) = 12.09, p < .001, ή2p = 0.09), Td (F(2, 246) = 3.39, p = .035, ή2p = 0.03), and Dm (F(2, 246) = 6.83, p = .001, ή2p = 0.05), while no significant effect of body side was observed. CONCLUSIONS: The findings indicate that muscle contraction time (Tc) and delay time (Td) are influenced by the time elapsed since caffeine ingestion, with the fastest responses occurring after 30 minutes. Additionally, a systemic effect of caffeine was observed, as there were no discernible differences in measurements between the two sides of the body. TMG proves to be an effective noninvasive method for assessing muscle responses following caffeine administration.

Efectos de un programa de ejercicios en casa para pacientes sometidos a hemodiálisis un estudio exploratorio / Effects of a home exercise program for patients undergoing hemodialysis an exploratory study

Antecedentes y objetivos: Los pacientes sometidos a hemodiálisis (HD) son particularmente vulnerables debido a las comorbilidades y a los fármacos inmunosupresores. En respuesta, este estudio tuvo como objetivo evaluar un programa alternativo de ejercicio en casa implementado para mantener la forma física de los pacientes en HD. Métodos: Se propuso un programa de entrenamiento de resistencia fuera de la clínica basado en ejercicios calisténicos a aquellos pacientes en HD que participaban en un programa de ejercicio intradialítico interrumpido durante diez semanas. Se aconsejó a los pacientes que realizaran una rutina de ejercicios en casa dos días a la semana. El impacto del programa sobre la función física se evaluó mediante pruebas que medían la fuerza muscular del tren superior e inferior, la aptitud cardiorrespiratoria y la autonomía funcional. Resultados: De los 53 pacientes elegibles para el estudio, 38 aceptaron participar y fueron asignados al grupo de ejercicio (n=17) o al grupo de control (n=21). Doce participantes completaron al menos el 80% de las sesiones de ejercicio y fueron incluidos en el análisis final. La intervención no tuvo efectos significativos intragrupo en las variables evaluadas. La comparación entre grupos indicó una mejora significativa en la fuerza muscular de la parte inferior del cuerpo, a favor del grupo de ejercicio. Conclusiones: Los resultados de este estudio exploratorio indican que un programa de ejercicios en casa realizado durante diez semanas ayudó a los pacientes con HD a prevenir el deterioro de la función muscular. (AU)

Histological characterization of the human masticatory oral mucosa. A histochemical and immunohistochemical study.

BACKGROUND: Histology of human oral mucosa is closely related with its function and anatomical location, and a proper characterization of the human masticatory oral mucosa could be very useful in periodontal pathology. OBJECTIVE: In the present work, we have carried out a comprehensive study in order to determine the main histological features of parakeratinized (POM) and orthokeratinized (OOM) masticatory human oral mucosa using light and electron microscopy. METHODS: To perform this, we have used several histological, histochemical and immunohistochemical methods to detect key markets at the epithelial, basement membrane and connective tissue levels. RESULTS: Our results demonstrated that POM and OOM share many histological similarities, as expected. However, important differences were observed at the epithelial layer of POM, that was significantly thicker than the epithelial layer found in OOM, especially due to a higher number of cells at the stratum spinosum. The expression pattern of CK10 and filaggrin revealed intense signal expression in OOM as compared to POM. Collagen and proteoglycans were more abundant in OOM stroma than in POM. No differences were found for blood vessels and basement membrane. CONCLUSION: These results may contribute to a better understanding of the pathological conditions affecting the human masticatory oral mucosa. In addition, these findings could be useful for the generation of different types of oral mucosa by tissue engineering techniques. RESEARCH HIGHLIGHTS: Microscopical features of parakeratinized and orthokeratinized masticatory human oral mucosa showed important differences at both, epithelial and stromal levels. Parakeratinized masticatory human oral mucosa exert thicker epithelial layer, especially, at the stratum spinosum in comparison to orthokeratinized human oral mucosa. Cytokeratin 10 and filaggrin human epithelial markers were intensively expressed in orthokeratinized masticatory human oral mucosa in comparison to parakeratinized masticatory human oral mucosa. At the stromal level, orthokeratinized masticatory human oral mucosa exhibit higher levels of collagen and proteoglycans than parakeratinized masticatory oral mucosa. The deep knowledge of histological features of masticatory oral mucosa could lead to a better understanding of oral mucosa pathology and advanced treatments.

Comprehensive ex vivo and in vivo preclinical evaluation of novel chemo enzymatic decellularized peripheral nerve allografts.

As a reliable alternative to autografts, decellularized peripheral nerve allografts (DPNAs) should mimic the complex microstructure of native nerves and be immunogenically compatible. Nevertheless, there is a current lack of decellularization methods able to remove peripheral nerve cells without significantly altering the nerve extracellular matrix (ECM). The aims of this study are firstly to characterize ex vivo, in a histological, biochemical, biomechanical and ultrastructural way, three novel chemical-enzymatic decellularization protocols (P1, P2 and P3) in rat sciatic nerves and compared with the Sondell classic decellularization method and then, to select the most promising DPNAs to be tested in vivo. All the DPNAs generated present an efficient removal of the cellular material and myelin, while preserving the laminin and collagen network of the ECM (except P3) and were free from any significant alterations in the biomechanical parameters and biocompatibility properties. Then, P1 and P2 were selected to evaluate their regenerative effectivity and were compared with Sondell and autograft techniques in an in vivo model of sciatic defect with a 10-mm gap, after 15 weeks of follow-up. All study groups showed a partial motor and sensory recovery that were in correlation with the histological, histomorphometrical and ultrastructural analyses of nerve regeneration, being P2 the protocol showing the most similar results to the autograft control group.

Biocompatible Short-Peptides Fibrin Co-assembled Hydrogels.

Fibrin hydrogels made by self-assembly of fibrinogen obtained from human plasma have shown excellent biocompatible and biodegradable properties and are widely used in regenerative medicine. The fibrinogen self-assembly process can be triggered under physiological conditions by the action of thrombin, allowing the injection of pregel mixtures that have been used as cell carriers, wound-healing systems, and bio-adhesives. However, access to fibrinogen from human plasma is expensive and fibrin gels have limited mechanical properties, which make them unsuitable for certain applications. One solution to these problems is to obtain composite gels made of fibrin and other polymeric compounds that improve their mechanical properties and usage. Herein, we prepared composite hydrogels made by the self-assembly of fibrinogen together with Fmoc-FF (Fmoc-diphenylalanine) and Fmoc-RGD (Fmoc-arginine-glycine-aspartic acid). We have shown that the mixture of these three peptides co-assembles and gives rise to a unique type of supramolecular fiber, whose morphology and mechanical properties can be modulated. We have carried out a complete characterization of these materials from chemical, physical, and biological points of view. Composite gels have improved mechanical properties compared to pure fibrin gels, as well as showing excellent biocompatibility ex vivo. In vivo experiments have shown that these gels do not cause any type of inflammatory response or tissue damage and are completely resorbed in short time, which would enable their use as vehicles for cell, drug, or growth factor release.

Intraseason Changes in Vertical Jumps of Male Professional Basketball Players.

This study aimed to analyze basketball players' jumping bhavior in the Squat Jump (SJ), Countermovement Jump (CMJ), and Free Arm Swing CMJ (CMJ Free) during a professional basketball season and check if it is modulated by the players' specific playing position, the time played on court, and the different leagues. Fifty-three male professional basketball players were assessed in three different moments of the season through SJ, CMJ, and CMJ Free. Between the beginning of pre-season (1st assessment) and the second round of the season (3rd assessment), there was a strong increase in performance in the three jumps (SJ Height: 5.6%, η2P = 0.234, p = 0.007; CMJ Height: 5.1%, η2P = 0.177, p = 0.007; CMJ Free height: 4.11%, η2P = 0.142, p = 0.01). There was also a significantly large increase in SJ and CMJ between the 2nd and 3rd assessments and in the CMJ Free between the 1st and 2nd assessments. No significant interactions were found between jumping performance and the group factors (players' specific playing position, time played on court, and league). In conclusion, SJ, CMJ, and CMJ Free performance strongly increases between 1st and 3rd assessment, without being influenced by the specific playing position or the minutes played per game.

Expression of Basement Membrane Molecules by Wharton Jelly Stem Cells (WJSC) in Full-Term Human Umbilical Cords, Cell Cultures and Microtissues.

Wharton's jelly stem cells (WJSC) from the human umbilical cord (UC) are one of the most promising mesenchymal stem cells (MSC) in tissue engineering (TE) and advanced therapies. The cell niche is a key element for both, MSC and fully differentiated tissues, to preserve their unique features. The basement membrane (BM) is an essential structure during embryonic development and in adult tissues. Epithelial BMs are well-known, but similar structures are present in other histological structures, such as in peripheral nerve fibers, myocytes or chondrocytes. Previous studies suggest the expression of some BM molecules within the Wharton's Jelly (WJ) of UC, but the distribution pattern and full expression profile of these molecules have not been yet elucidated. In this sense, the aim of this histological study was to evaluate the expression of main BM molecules within the WJ, cultured WJSC and during WJSC microtissue (WJSC-MT) formation process. Results confirmed the presence of a pericellular matrix composed by the main BM molecules-collagens (IV, VII), HSPG2, agrin, laminin and nidogen-around the WJSC within UC. Additionally, ex vivo studies demonstrated the synthesis of these BM molecules, except agrin, especially during WJSC-MT formation process. The WJSC capability to synthesize main BM molecules could offer new alternatives for the generation of biomimetic-engineered substitutes where these molecules are particularly needed.

Peripheral nerve regeneration through nerve conduits evokes differential expression of growth-associated protein-43 in the spinal cord.

Growth-associated protein 43 plays a key role in neurite outgrowth through cytoskeleton remodeling. We have previously demonstrated that structural damage of peripheral nerves induces growth-associated protein 43 upregulation to promote growth cone formation. Conversely, the limited regenerative capacity of the central nervous system due to an inhibitory environment prevents major changes in neurite outgrowth and should be presumably associated with low levels of growth-associated protein 43 expression. However, central alterations due to peripheral nerve damage have never been assessed using the growth-associated protein 43 marker. In this study, we used the tubulization technique to repair 1 cm-long nerve gaps in the rat nerve injury/repair model and detected growth-associated protein 43 expression in the peripheral and central nervous systems. First, histological analysis of the regeneration process confirmed an active regeneration process of the nerve gaps through the conduit from 10 days onwards. The growth-associated protein 43 expression profile varied across regions and follow-up times, from a localized expression to an abundant and consistent expression throughout the regeneration tissue, confirming the presence of an active nerve regeneration process. Second, spinal cord changes were also histologically assessed, and no apparent changes in the structural and cellular organization were observed using routine staining methods. Surprisingly, remarkable differences and local changes appeared in growth-associated protein 43 expression at the spinal cord level, in particular at 20 days post-repair and beyond. Growth-associated protein 43 protein was first localized in the gracile fasciculus and was homogeneously distributed in the left posterior cord. These findings differed from the growth-associated protein 43 pattern observed in the healthy control, which did not express growth-associated protein 43 at these levels. Our results revealed a differential expression in growth-associated protein 43 protein not only in the regenerating nerve tissue but also in the spinal cord after peripheral nerve transection. These findings open the possibility of using this marker to monitor changes in the central nervous system after peripheral nerve injury.

Neuromuscular changes after a Long Distance Triathlon World Championship.

ABSTRACTThis study aimed to determine the neuromuscular changes in muscle contractile properties and countermovement jump (CMJ) performance after a long distance (LD) triathlon, inquiring to what extent it is influenced by gender and the evaluated muscle, and to explore for response differences. Four elite (2 females and 2 males) and 18 well-trained triathletes (3 females and 15 males) who competed in the 2019 LD Triathlon World Championship undergone, prior and post-race, a CMJ and a muscle contractile properties assessment in vastus lateralis (VL) and biceps femoris (BF) using tensiomyography. A mixed ANOVA was carried out to detect triathletes' neuromuscular changes after the competition. A large decrease in jump height and flight time was found in CMJ (p = 0.001, ηp2 = 0.767) with gender differences (ηp2 = 0.308). contraction (Tc), delay (Td) and sustain times (Ts) also showed a large decrease (p = 0.001, ηp2 = 0.174-0.413). However, maximum radial muscle belly displacement was not altered by the competition, but did greatly increase radial displacement velocity (p = 0.031; ηp2 = 0.212). No gender differences were found in contractile properties. Correlation analysis between race performance and the jumping and tensiomyography parameters only showed a moderate negative correlation with Tc of the BF in pre- and post-competition (r = -0.44; -0.43; p = 0.05). In conclusion, triathletes after a LD World Championship suffer a sharp drop in jump performance and variations in VL's and BF's contractile properties, showing decreases in Tc, Td and Ts without gender differences. The regular use of these parameters can allow the monitoring of triathlete's neuromuscular fatigue and aid in the distribution of more efficient loads in their training cycles.HighlightsTrainers and researchers in neuromuscular fatigue can have reference data of elite and well-trained triathletes after performing LD race.The magnitude of change in the CMJ and the contractile properties of BF and VL achieved in competition by these elite reference triathletes can guide to modulate triathletes training.The regular use of the parameters using these references can allow an exhaustive neuromuscular fatigue monitoring during the sessions or training cycles throughout the season.

Chitosan conduits enriched with fibrin-collagen hydrogel with or without adipose-derived mesenchymal stem cells for the repair of 15-mm-long sciatic nerve defect.

Hollow conduits of natural or synthetic origins have shown acceptable regeneration results in short nerve gap repair; however, results are still not comparable with the current gold standard technique "autografts". Hollow conduits do not provide a successful regeneration outcome when it comes to critical nerve gap repair. Enriching the lumen of conduits with different extracellular materials and cells could provide a better biomimicry of the natural nerve regenerating environment and is expected to ameliorate the conduit performance. In this study, we evaluated nerve regeneration in vivo using hollow chitosan conduits or conduits enriched with fibrin-collagen hydrogels alone or with the further addition of adipose-derived mesenchymal stem cells in a 15 mm rat sciatic nerve transection model. Unexpected changes in the hydrogel consistency and structural stability in vivo led to a failure of nerve regeneration after 15 weeks. Nevertheless, the molecular assessment in the early regeneration phase (7, 14, and 28 days) has shown an upregulation of useful regenerative genes in hydrogel enriched conduits compared with the hollow ones. Hydrogels composed of fibrin-collagen were able to upregulate the expression of soluble NRG1, a growth factor that plays an important role in Schwann cell transdifferentiation. The further enrichment with adipose-derived mesenchymal stem cells has led to the upregulation of other important genes such as ErbB2, VEGF-A, BDNF, c-Jun, and ATF3.

Predicting Hamstring Strains in Soccer Players Based on ROM: An Analysis From a Gender Perspective.

Purpose: The aim of this study was to explore if specific hip and knee range-of-movement (ROM) tests can predict a risk factor for hamstring strain (HS) injury in male and female soccer players. Methods: One hundred amateur soccer players (56 men and 44 women) performed six tests to determine hip and knee ROM: straight leg raise test (SLR), modified Thomas test (TT), hip internal and external rotation (ER), hip abduction and adduction, Nachlas test and Ridge test. A logistic regression analysis was carried out to create a predictive model for HS injuries. Results: The percentage of HS injury was 20.45% and 30.35%, for female and male players. The logistic regression showed a significant model for both genders on the logit of suffering an HS injury with active-SLR and TT variables for females (R2CS = 0.491; R2N = 0.771) and active SLR and ER variables for males (R2CS = 0.623; R2N = 0.882). The predictive models correctly classify 95.5% and 94.6% of cases presenting good sensitivity (77.8% and 88.2%) and full (100%) and high (97.4%) specificity respectively. Furthermore, female players showed a greater ROM than males (p ≤ 0.01). Conclusion: Both female and male soccer players that suffered a HS injury had lower ROM in SLR, NT and RT and higher ROM in the TT that non-injured players. The tests that most likely predict HS injury are SLR and TT in females and SLR and ER in males. Thus, it is suggested to including specific exercises in amateur soccer players training programs to improve hip and knee ROM for injury prevention.

Identification of histological threshold concepts in health sciences curricula: Students' perception.

Students' metacognitive skills and perceptions are considered important variables for high-quality learning. In this study, students' perceptions were used to identify histological threshold concepts (integrative, irreversible, transformative, and troublesome) in three health sciences curricula. A specific questionnaire was developed and validated to characterize students' perceptions of histological threshold concepts. A sample of 410 undergraduate students enrolled in the dentistry, medicine, and pharmacy degree programs participated in the study. Concepts assessed in the study were clustered to ten categories (factors) by exploratory and confirmatory factor analysis. Concepts linked to tissue organization and tissue functional states received the highest scores from students in all degree programs, suggesting that the process of learning histology requires the integration of both static concepts related to the constituent elements of tissues and dynamic concepts such as stem cells as a tissue renewal substrate, or the euplasic, proplasic and retroplasic states of tissues. The complexity of integrating static and dynamic concepts may pose a challenging barrier to the comprehension of histology. In addition, several differences were detected among the students in different degree programs. Dentistry students more often perceived morphostructural concepts as threshold concepts, whereas medical students highlighted concepts related to two-dimensional microscopic identification. Lastly, pharmacy students identified concepts related to tissue general activity as critical for the comprehension and learning of histology. The identification of threshold concepts through students' perceptions is potentially useful to improve the teaching and learning process in health sciences curricula.

Staining Methods for Normal and Regenerative Myelin in the Nervous System.

Histochemical and fluorescence-based techniques enable the specific identification of myelin by bright-field or fluorescence microscopy. In this chapter, we describe four histological methods for the evaluation of myelin on peripheral nerve tissue sections. The first method combines the Luxol fast blue (LFB) technique with a modified Picrosirius staining contrasted with Harris hematoxylin, called MCOLL. This method simultaneously stains myelin, collagen fibers, and cell nuclei, thus giving an integrated overview of the histology, collagen network, and myelin content of the tissue in paraffin-embedded or cryosectioned samples. Secondly, we describe the osmium tetroxide method, which provides a permanent positive reaction for myelin as well as other lipids present in the tissue. The third method is the immunofluorescence-based detection of myelin proteins that allows to combine information about their expression status with other proteins of interest. Finally, the FluoroMyelin™ stains enable a fast detection of the myelin content that can be easily implemented in immunofluorescence staining panels for cryosectioned tissues. Together, this chapter provides a variety of methods to accurately identify myelin in different experimental approaches.

Individualized Analysis of Lateral Asymmetry Using Hip-Knee Angular Measures in Soccer Players: A New Methodological Perspective of Assessment for Lower Limb Asymmetry.

This study aimed to: (1) determine the magnitude and direction of lateral asymmetry in well-trained soccer players using hip and knee ROM tests; (2) inquire if asymmetry relies on the ROM test performed and/or gender; and (3) establish asymmetry thresholds for each ROM test to individualize lower-limbs asymmetry. One hundred amateur soccer players were assessed using hip-knee ROM tests: Straight Leg Raise, modified Thomas Test, hip internal rotation and external rotation, hip abduction (ABD) and adduction (ADD), Nachlas Test and Rigde Test. There are significant differences between tests when determining the magnitude of lateral asymmetry (F = 3.451; p = 0.001; ηp2 = 0.031) without significant differences between gender (F = 0.204; p = 0.651; ηp2 = 0.001). Asymmetry threshold results differ significantly between using a fixed or a specific threshold (F = 65.966; p = 0.001; ηp2 = 0.985). All tests indicate that the direction of asymmetry is towards the dominant limb. In conclusion, the ROM test used determines the magnitude and direction of the lateral asymmetry of the amateur soccer players. The ABD and ADD are the ROM tests that showed higher percentages of asymmetry, without differences between female and male soccer players. Using a specific asymmetry threshold formula can classify more players as asymmetrical than with a fixed threshold.

Interday Reliability of a Testing Battery to Assess Lateral Symmetry and Performance in Well-Trained Volleyball Players.

ABSTRACT: Iglesias-Caamaño, M, Álvarez-Yates, T, Carballo-López, J, Cuba-Dorado, A, and García-García, O. Interday reliability of a testing battery to assess lateral symmetry and performance in well-trained volleyball players. J Strength Cond Res 36(4): 895-901, 2022-This study aimed to determine the relative and absolute interday reliability of a testing battery for lateral asymmetry detection and performance in well-trained volleyball players and to determine whether retaining for analysis the best or the average value of 3 attempts would modulate the interday reliability and the magnitude of asymmetry. Thirteen Superleague-2 male volleyball players performed on 3 different days a 5-test battery composed of 4 tests for assessing muscle asymmetries: active knee extension, Y-balance test (YBT), modified 20-yard shuttle run (M-20Y), and single-leg countermovement jump; and one for volleyball sports performance: attack jump (AJ). The intraclass correlation index and coefficient of variation (CV) together with the standard error of measurement (SEM, %SEM) and the minimum detectable change (MDC, %MDC) were taken to calculate relative and absolute reliability. Two-way repeated-measures analysis of variance was used to establish the differences between the best value and the average value in each session. The low %MDC (<10%) suggests that all the tests that make up the testing battery would have good sensitivity to detect possible asymmetries. All tests have shown an excellent interday absolute reliability (CV <4.5%; %SEM < 5%), both when retaining the best value and the average value of the 3 attempts. In addition, AJ and YBT (on both legs) obtained a good-excellent relative reliability, whereas the M-20Y test seems to show a lower relative reliability. Retaining the best value or the average value to determine the magnitude and direction of asymmetry seems to be equally reliable with no differences between both values.

Elite Triathlete Profiles in Draft-Legal Triathlons as a Basis for Talent Identification.

Draft-legal triathlons are the main short-distance races worldwide and are those on which talent-identification programs are usually focused. Performance in these races depends on multiple factors; however, many investigations do not focus on elite triathletes. Therefore, the aim of this narrative review was to carry out a systematic literature search to define the elite female and male triathlete profiles and their competition demands in draft-legal triathlons. This will allow us to summarize the main determinant factors of high-level triathletes as a basis for talent detection. A comprehensive review of Web of Science and Scopus was performed using the search strategy: Triathl* and (performance or competition or profile) and (elite or professional or "high performance" or "high level" or talent). A total of 1325 research documents were obtained, and after screening following the criteria, only 83 articles were selected. After data synthesis, elite triathlete aspects such as age, physiological, anthropometric, and psychosocial profile or competition demands were studied in the scientific literature. Thus, it is essential that when implementing talent identification programs, these factors must be considered. However, constant updating is needed due the continuous regulatory changes and the need of triathletes to adapt to these new competition demands.

Acute Mechanical, Neuromuscular, and Metabolic Responses to Different Set Configurations in Resistance Training.

ABSTRACT: Piqueras-Sanchiz, F, Cornejo-Daza, PJ, Sánchez-Valdepeñas, J, Bachero-Mena, B, Sánchez-Moreno, M, Martín-Rodríguez, S, García-García, Ó, and Pareja-Blanco, F. Acute mechanical, neuromuscular, and metabolic responses to different set configurations in resistance training. J Strength Cond Res 36(11): 2983-2991, 2022-The aim of this study was to investigate the effect of set configuration on mechanical performance, neuromuscular activity, metabolic response, and muscle contractile properties. Sixteen strength-trained men performed 2 training sessions in the squat exercise consisting of (a) 3 sets of 8 repetitions with 5 minutes rest between sets (3 × 8) and (b) 6 sets of 4 repetitions with 2 minutes rest between sets (6 × 4). Training intensity (75% one repetition maximum), total volume (24 repetitions), total rest (10 minutes), and training density were equalized between protocols. A battery of tests was performed before and after each protocol: (a) tensiomyography (TMG), (b) blood lactate and ammonia concentration, (c) countermovement jump, and (d) maximal voluntary isometric contraction in the squat exercise. Force, velocity, and power output values, along with electromyography data, were recorded for every repetition throughout each protocol. The 6 × 4 protocol resulted in greater mechanical performance (i.e., force, velocity, and power) and lower neuromuscular markers of fatigue (i.e., lower root mean square and higher median frequency) during the exercise compared with 3 × 8, particularly for the last repetitions of each set. The 3 × 8 protocol induced greater lactate and ammonia concentrations, greater reductions in jump height, and greater impairments in TMG-derived velocity of deformation after exercise than 6 × 4. Therefore, implementing lower-repetition sets with shorter and more frequent interset rest intervals attenuates impairments in mechanical performance, especially in the final repetitions of each set. These effects may be mediated by lower neuromuscular alterations, reduced metabolic stress, and better maintained muscle contractile properties.