RESUMO
Huanglongbing, also known as citrus greening, is currently the most devastating citrus disease with limited success in prevention and mitigation. A promising strategy for Huanglongbing control is the use of antimicrobials fused to a carrier protein (phloem protein of 16 kDa or PP16) that targets vascular tissues. This study investigated the effects of genetically modified citrus trees expressing Citrus sinensis PP16 (CsPP16) fused to human lysozyme and ß-defensin-2 on the soil microbiome diversity using 16S amplicon analysis. The results indicated that there were no significant alterations in alpha diversity, beta diversity, phylogenetic diversity, differential abundance, or functional prediction between the antimicrobial phloem-overexpressing plants and the control group, suggesting minimal impact on microbial community structure. However, microbiota diversity analysis revealed distinct bacterial assemblages between the rhizosphere soil and root environments. This study helps to understand the ecological implications of crops expressing phloem-targeted antimicrobials for vascular disease management, with minimal impact on soil microbiota.
Assuntos
Bactérias , Citrus , Microbiota , Floema , Doenças das Plantas , Rizosfera , Microbiologia do Solo , Floema/microbiologia , Floema/metabolismo , Bactérias/genética , Bactérias/classificação , Bactérias/metabolismo , Bactérias/isolamento & purificação , Doenças das Plantas/microbiologia , Citrus/microbiologia , Plantas Geneticamente Modificadas/microbiologia , Plantas Geneticamente Modificadas/genética , Filogenia , Metagenômica , Muramidase/metabolismo , Muramidase/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , beta-Defensinas/genética , RNA Ribossômico 16S/genética , Anti-Infecciosos/farmacologia , Anti-Infecciosos/metabolismo , Citrus sinensis/microbiologia , Raízes de Plantas/microbiologiaRESUMO
The use of metallic nanowires is mostly reduced to scientific areas where a small quantity of nanostructures are needed. In order to broaden the applicability of these nanomaterials, it is necessary to establish novel synthesis protocols that provide a larger amount of nanowires than the conventional laboratory fabrication processes at a more competitive cost. In this work, we propose several modifications to the conventional electrochemical synthesis of nanowires in order to increase the production with considerably reduced production time and cost. To that end, we use a soft anodization procedure of recycled aluminum at room temperature to produce the alumina templates, followed by galvanostatic growth of CoFe nanowires. We studied their morphology, composition and magnetic configuration, and found that their properties are very similar to those obtained by conventional methods.