Doses and spectra, of leaking-out and scattered photons from X-ray tubes used in dentistry.

Diagnosis by X-ray image are procedures widely used to improve the diagnosis or to follow the evolution of a medical procedure, also are used to support the cancer treatment with linear accelerators. The procedure involves the X-ray unit and the detection system, while the X-ray beam is projected onto the patient; along this procedure X-rays are scattered out from the patient body and X-rays leak-out from the X-ray unit. Both, the scattered and the leaking out radiation, are important during the evaluation or the design of the shielding barriers. X-ray tube features like voltage, electric current, target and filter are related to the dose due to the scattered and leakage radiation. Also, the dose due to the scattered radiation depends upon the scattering angle; while the dose due to the leaking out radiation, depend upon the X-ray tube housing. The objective of this work was to estimate, using Monte Carlo methods, the X-ray spectra and doses (air Kerma and Ambient dose equivalent) produced by scattered and leaking out radiation of 70, 80 and 90 kV X-ray units.

Mathematical description of the effect of HIF inhibition on the radiobiological response of LNCaP cells.

According to the National Institute of Public Health, prostate cancer (PCa) is the leading cause of cancer death in Mexican men, highly associated with aggressiveness, resistance to treatment, and metastatic spread (Bharti et al., 2019) mediated by activation of the hypoxia-inducible factor 1 (HIF-1α). The objective of the present study was to evaluate the participation of HIF-1α activation in the radiobiological response of the human prostate adenocarcinoma cell line LNCaP, describing the phenomena with a mathematical model. Four groups were formed under different exposure conditions, including hypoxic cells treated with CoCl2 (300 µM for 22 h) with or without hypoxia-inducible factor inhibitor (150 nM chetomin for 4 h added after an incubation period of 18 h with CoCl2, just before completing the incubation period of 22 h). They were exposed to a source of 60Co in a dose range between 2 and 10 Gy to obtain survival curves that are fitted to a mathematical model. CoCl2 or chetomin treatments do not affect the viability of LNCaP cells that remained unchanged after irradiation. CoCl2 induced hypoxia reduces the survivability of LNCaP, and obstruction of HIF-1α signaling with chetomine produces a slight radioprotective effect. As others report, the genetic reprogramming induced by HIF-1α activation acts as an intrinsic agent that selects cells with more aggressive behavior (Pressley et al., 2017), while chetomin protects cells from death due to its scavenger properties. Interestingly, treatment with chetomin of cells induced to hypoxia (HIF-1 activation with CoCl2) produces a significant reduction in the radioresistance of LNCaP cells, demonstrating that the simultaneous use of chetomin and gamma radiation is an effective option for the treatment of hypoxic prostate cancer. At the molecular level, we suggest that the selective force exerted by HIF-1α depends on the production of free radicals by radiation. The proposed mathematical model showed that the rate of change in cell survival as a function of radiation dose is proportional to the product of two functions, one that describes cell death and the other that describes natural or artificial resistance to radiation.