Acute toxicity and bioaccumulation of common urban metals in Bombus impatiens life stages.

Metal contamination is ubiquitous in urban areas and represents a risk to arthropod species. Bees are exposed to metals while foraging within contaminated landscapes from multiple sources. Eliminating the risk of bee exposure to metals is complex, and requires an understanding of how bees become contaminated, how metals accumulate within bee bodies, and how this exposure influences their health. We selected Bombus impatiens, the common eastern bumble bee, as our focal species because it is the most frequently encountered bumble bee species in the eastern United States and common within urban greenspaces. The aims of this study were to quantify the lethal concentration exposure limit (LC50) for B. impatiens foragers, assess the bioaccumulation ability of environmentally relevant concentrations of common urban metals in adults, larvae, and pupae, and compare the LC50 values against field relevant concentrations collected by foraging bumble bees within a legacy city. Bumble bees were orally exposed to arsenic oxide, cadmium chloride, or chromium oxide in sucrose solution to encourage consumption. The LC50 for arsenic (As2O3 36.4 mg/L), cadmium (CdCl2 10.3 mg/L), and chromium (CrO3 189.6 mg/L) are 202×, 79×, and 1459× greater than concentrations found within urban bumble bee collected provisions, respectively. Adult bumble bees fed field realistic concentrations of metals accumulate significant amounts of cadmium and lead within their bodies, but do not accumulate chromium and arsenic. Additionally, adults accumulate significantly higher concentrations of metals than brood. While bumble bee foragers are unlikely to encounter lethal metal concentrations while foraging in contaminated landscapes, it is crucial to consider and understand how sublethal concentrations impact overall colony functioning. The results from this study highlight the need to identify hazards and bioaccumulation ability of common metals as bees respond differently to each metal species, as well as the impacts of metal mixtures on bioaccumulation and toxicity.

Potential for urban warming to postpone overwintering dormancy of temperate mosquitoes.

Cities are generally hotter than surrounding rural areas due to the Urban Heat Island (UHI) effect. These increases in temperature advance plant and animal phenology, development, and reproduction in the spring. However, research determining how increased temperatures affect the seasonal physiology of animals in the fall has been limited. The Northern house mosquito, Culex pipiens, is abundant in cities and transmits several pathogens including West Nile virus. Females of this species enter a state of developmental arrest, or reproductive diapause, in response to short days and low temperatures during autumn. Diapausing females halt reproduction and blood-feeding, and instead accumulate fat and seek sheltered overwintering sites. We found that exposure to increased temperatures in the lab that mimic the UHI effect induced ovarian development and blood-feeding, and that females exposed to these temperatures were as fecund as non-diapausing mosquitoes. We also found that females exposed to higher temperatures had lower survival rates in winter-like conditions, despite having accumulated equivalent lipid reserves relative to their diapausing congeners. These data suggest that urban warming may inhibit diapause initiation in the autumn, thereby extending the active biting season of temperate mosquitoes.

Sustained VWF-ADAMTS-13 axis imbalance and endotheliopathy in long COVID syndrome is related to immune dysfunction.

BACKGROUND: Prolonged recovery is common after acute SARS-CoV-2 infection; however, the pathophysiological mechanisms underpinning Long COVID syndrome remain unknown. VWF/ADAMTS-13 imbalance, dysregulated angiogenesis, and immunothrombosis are hallmarks of acute COVID-19. We hypothesized that VWF/ADAMTS-13 imbalance persists in convalescence together with endothelial cell (EC) activation and angiogenic disturbance. Additionally, we postulate that ongoing immune cell dysfunction may be linked to sustained EC and coagulation activation. PATIENTS AND METHODS: Fifty patients were reviewed at a minimum of 6 weeks following acute COVID-19. ADAMTS-13, Weibel Palade Body (WPB) proteins, and angiogenesis-related proteins were assessed and clinical evaluation and immunophenotyping performed. Comparisons were made with healthy controls (n = 20) and acute COVID-19 patients (n = 36). RESULTS: ADAMTS-13 levels were reduced (p = 0.009) and the VWF-ADAMTS-13 ratio was increased in convalescence (p = 0.0004). Levels of platelet factor 4 (PF4), a putative protector of VWF, were also elevated (p = 0.0001). A non-significant increase in WPB proteins Angiopoietin-2 (Ang-2) and Osteoprotegerin (OPG) was observed in convalescent patients and WPB markers correlated with EC parameters. Enhanced expression of 21 angiogenesis-related proteins was observed in convalescent COVID-19. Finally, immunophenotyping revealed significantly elevated intermediate monocytes and activated CD4+ and CD8+ T cells in convalescence, which correlated with thrombin generation and endotheliopathy markers, respectively. CONCLUSION: Our data provide insights into sustained EC activation, dysregulated angiogenesis, and VWF/ADAMTS-13 axis imbalance in convalescent COVID-19. In keeping with the pivotal role of immunothrombosis in acute COVID-19, our findings support the hypothesis that abnormal T cell and monocyte populations may be important in the context of persistent EC activation and hemostatic dysfunction during convalescence.

The Role of Community Science in Entomology.

Community (or citizen) science, the involvement of volunteers in scientific endeavors, has a long history. Over the past few centuries, the contributions of volunteers to our understanding of patterns and processes in entomology have been inspiring. From the collation of large-scale and long-term data sets, which have been instrumental in underpinning our knowledge of the status and trends of many insect groups, to action, including species management, whether for conservation or control, community scientists have played pivotal roles. Contributions, such as pest monitoring by farmers and species discoveries by amateur naturalists, set foundations for the research engaging entomologists today. The next decades will undoubtedly bring new approaches, tools, and technologies to underpin community science. The potential to increase inclusion within community science is providing exciting opportunities within entomology. An increase in the diversity of community scientists, alongside an increasing taxonomic and geographic breadth of initiatives, will bring enormous benefits globally for people and nature.

Longitudinal Analysis of COVID-19 Patients Shows Age-Associated T Cell Changes Independent of Ongoing Ill-Health.

Objectives: The immunological and inflammatory changes following acute COVID-19 are hugely variable. Persistent clinical symptoms following resolution of initial infection, termed long COVID, are also hugely variable, but association with immunological changes has not been described. We investigate changing immunological parameters in convalescent COVID-19 and interrogate their potential relationships with persistent symptoms. Methods: We performed paired immunophenotyping at initial SARS-CoV-2 infection and convalescence (n=40, median 68 days) and validated findings in 71 further patients at median 101 days convalescence. Results were compared to 40 pre-pandemic controls. Fatigue and exercise tolerance were assessed as cardinal features of long COVID using the Chalder Fatigue Scale and 6-minute-walk test. The relationships between these clinical outcomes and convalescent immunological results were investigated. Results: We identify persistent expansion of intermediate monocytes, effector CD8+, activated CD4+ and CD8+ T cells, and reduced naïve CD4+ and CD8+ T cells at 68 days, with activated CD8+ T cells remaining increased at 101 days. Patients >60 years also demonstrate reduced naïve CD4+ and CD8+ T cells and expanded activated CD4+ T cells at 101 days. Ill-health, fatigue, and reduced exercise tolerance were common in this cohort. These symptoms were not associated with immune cell populations or circulating inflammatory cytokines. Conclusion: We demonstrate myeloid recovery but persistent T cell abnormalities in convalescent COVID-19 patients more than three months after initial infection. These changes are more marked with age and are independent of ongoing subjective ill-health, fatigue and reduced exercise tolerance.

Effects of urban greenspace configuration and native vegetation on bee and wasp reproduction.

Pollinator welfare is a recognized research and policy target, and urban greenspaces have been identified as important habitats. Yet, landscape-scale habitat fragmentation and greenspace management practices may limit a city's conservation potential. We examined how landscape configuration, composition, and local patch quality influenced insect nesting success across inner-city Cleveland, Ohio (U.S.A.), a postindustrial legacy city containing a high abundance of vacant land (over 1600 ha). Here, 40 vacant lots were assigned 1 of 5 habitat treatments (T1, vacant lot; T2, grass lawn; T3, flowering lawn; T4, grass prairie; and T5, flowering prairie), and we evaluated how seeded vegetation, greenspace size, and landscape connectivity influenced cavity-nesting bee and wasp reproduction. Native bee and wasp larvae were more abundant in landscapes that contained a large patch (i.e., >6 ha) of contiguous greenspace, in habitats with low plant biomass, and in vacant lots seeded with a native wildflower seed mix or with fine-fescue grass, suggesting that fitness was influenced by urban landscape features and habitat management. Our results can guide urban planning by demonstrating that actions that maintain large contiguous greenspace in the landscape and establish native plants would support the conservation of bees and wasps. Moreover, our study highlights that the world's estimated 350 legacy cities are promising urban conservation targets due to their high abundance of vacant greenspace that could accommodate taxa's habitat needs in urban areas.

Efectos de la Configuración de Espacios Verdes Urbanos y la Vegetación Nativa sobre la Reproducción de Abejas y Avispas Resumen El bienestar de los polinizadores es un objetivo reconocido de la investigación y las políticas, y los espacios verdes urbanos han sido identificados como hábitats importantes para esto. Aun así, la fragmentación de hábitat a escala de paisaje y las prácticas de manejo de los espacios verdes pueden limitar el potencial de conservación de una ciudad. Analizamos cómo la configuración del paisaje, la composición y la calidad del fragmento local influyen sobre el éxito de anidación de los insectos a lo largo del casco urbano de Cleveland, Ohio (E.U.A.), una ciudad con legado postindustrial que contiene una abundancia alta de terrenos baldíos (más de 1,600 ha). En este estudio, se les asignó a 40 lotes baldíos uno de cinco tratamientos de hábitat (T1, lote baldío; T2, jardín de césped; T3, jardín con flores; T4, pradera de césped; T5, pradera con flores) y evaluamos cómo la vegetación sembrada, el tamaño del espacio verde y la conectividad del paisaje influyeron sobre la reproducción de abejas y avispas que anidan en cavidades. Las larvas de abejas y avispas nativas fueron más abundantes en los paisajes con un fragmento grande de espacio verde contiguo (es decir, >6 ha), en hábitats con baja biomasa vegetal y en lotes baldíos con sembrados de una mezcla de flores silvestres nativas y césped de festuca fina, lo que sugiere que la aptitud estuvo influenciada por las características del paisaje urbano y el manejo del hábitat. Nuestros resultados pueden guiar a la planeación urbana mediante la demostración de acciones que mantienen un gran espacio verde contiguo dentro del paisaje y establecer que las plantas nativas podrían apoyar en la conservación de abejas y avispas. Además, nuestro estudio resalta que las 350 ciudades de legado postindustrial estimadas son objetivos prometedores de conservación debido a la alta abundancia que presentan de espacio verde vacante, el cual podría acomodar las necesidades de hábitat de los taxones en las áreas urbanas.

Community science data suggests that urbanization and forest habitat loss threaten aphidophagous native lady beetles.

Community scientists have illustrated rapid declines of several aphidophagous lady beetle (Coccinellidae) species. These declines coincide with the establishment of alien coccinellids. We established the Buckeye Lady Beetle Blitz program to measure the seasonal occupancy of coccinellids within gardens across a wide range of landscape contexts. Following the Habitat Compression Hypothesis, we predicted that gardens within agricultural landscapes would be alien-dominated, whereas captures of natives would be higher within landscapes encompassing a high concentration of natural habitat.Within the state of Ohio, USA, community scientists collected lady beetles for a 7-day period across 4 years in June and August using yellow sticky card traps. All identifications were verified by professional scientists and beetles were classified by three traits: status (alien or native), mean body length, and primary diet. We compared the relative abundance and diversity of coccinellids seasonally and determined if the distribution of beetles by size, status, and diet was related to landscape features.Alien species dominated the aphidophagous fauna. Native aphidophagous coccinellid abundance was positively correlated with forest habitat while alien species were more common when gardens were embedded within agricultural landscapes. Urbanization was negatively associated with both aphidophagous alien and native coccinellids. Synthesis and Applications: Our census of native coccinellid species within residential gardens-a widespread and understudied habitat-was enabled by volunteers. These data will serve as an important baseline to track future changes within coccinellid communities within this region. We found that native coccinellid species richness and native aphidophagous coccinellid abundance in gardens were positively associated with forest habitat at a landscape scale of 2 km. However, our understanding of when and why (overwintering, summer foraging, or both) forest habitats are important remains unclear. Our findings highlight the need to understand how declining aphidophagous native species utilize forest habitats as a conservation priority.

Comparison of SARS-CoV-2 IgM and IgG seroconversion profiles among hospitalized patients in two US cities.

The clinical and public health utility of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serologic testing requires a better understanding of the dynamics of the humoral response to infection. To track seroconversion of IgG and IgM antibodies in patients with SARS-CoV-2 infection and its association with patient and clinical factors and outcomes. Residual patient specimens were analyzed on the Abbott ARCHITECT i2000 instrument using the Abbott SARS-CoV-2 IgG assay and prototype SARS-CoV-2 IgM assay. Age, sex, comorbidities, symptom onset date, mortality, and specimen collection date were obtained from electronic medical records. Three hundred fifty-nine longitudinal samples were collected from 89 hospitalized patients 0 to 82 days postsymptom onset. Of all, 51.7% of the patients developed IgG and IgM antibodies simultaneously; 32.8% seroconverted for IgM before IgG. On average, patients seroconverted for IgG by 8 days and for IgM by 7 days postsymptom onset. All patients achieved IgG seropositivity by 19 days and IgM seropositivity by 17 days. Median time to IgG and IgM seroconversion was prolonged and initial levels of IgG were lower in immunocompromised patients and patients <65 years of age compared to immune competent patients and those ≥65 years of age. Immunocompromised patients also had persistently lower levels of IgM that peaked on day 17.6 and decreased thereafter compared to immune competent patients. IgM seroconversion in patients who died reached significantly higher levels later after symptom onset than in those who recovered. SARS-CoV-2 infected patients have similar time to seroconversion for IgG and IgM. However, differences in immune status and age alter time to seroconversion. These results may help guide serologic testing application in COVID-19 management.

Artificial light at night alters the seasonal responses of biting mosquitoes.

Urban light pollution caused by artificial light at night (ALAN) profoundly affects the ecology, behavior, and physiology of plants and animals. Further, this widespread environmental pollutant has the potential to negatively impact human and animal health by changing the seasonal dynamics of disease-transmitting insects. In response to short days, females of the Northern house mosquito enter an overwintering dormancy, or diapause. While in diapause, female mosquitoes divert energy away from reproduction, cease blood-feeding, and no longer transmit disease. We demonstrate that exposure to dim ALAN (~4 lx) causes female mosquitoes to avert diapause and become reproductively active, as these females acquired less fat content, developed larger egg follicles, imbibed vertebrate blood, and produced viable eggs and larvae. Our findings suggest that mosquitoes in highly light-polluted areas such as cities may be actively reproducing and biting later in the season, thereby extending the period of disease risk for urban residents. Our results suggest that ALAN should be considered when modeling mosquito abundance, disease risk, and when deciding how long mosquito surveillance and control should persist in temperate regions.

Rich and abundant spider communities result from enhanced web capture breadth and reduced overlap in urban greenspaces.

Urbanization is a key contributor to biodiversity loss, but evidence is mounting that cities can support rich arthropod communities, including rare and threatened species. Furthermore, greenspace is growing within hundreds of "shrinking cities" that have lost population resulting in a need to demolish an overabundance of infrastructure creating vacant land. Efforts are underway to transform vacant lots, often viewed as blighted areas, into habitats that promote biodiversity and generate ecosystem services, such as urban agroecosystems. To understand how reconfiguring these greenspaces might influence species conservation, elucidation of the factors that drive the distribution of an urban species pool is needed. In particular, the importance of species interactions in structuring urban communities is poorly understood. We tested hypotheses that (1) greater breadth of prey captured by web-building spiders and reduced overlap of prey capture among individuals facilitates the conservation of genera richness and abundance and (2) heterogeneity within a greenspace patch facilitates enhanced dietary niche breadth and greater resource partitioning. In 2013 and 2014, the abundance, breadth and degree of overlap in prey capture of sheet web spiders (Linyphiidae) was measured using web mimic traps at 160 microsites (0.25 m2 ) situated in four urban vacant lots and four urban farms in the city of Cleveland, Ohio, USA. Within a subset of 40 microsites, we used vacuum sampling and hand collection to measure the abundance and genera richness of Linyphiidae. Spider richness and abundance were significantly reduced within urban farms relative to vacant lots. The distribution of spiders and prey was explained by habitat structure, with microsites dominated by tall grasses and flowering plants, with a high bloom abundance and richness, supporting greater prey capture and a higher genera richness and abundance of spiders. In 2014, web capture overlap was significantly greater within microsites dominated by bare ground. These findings illustrate that urban greenspace conservation efforts that focus on reducing bare ground and incorporating a diversity of grasses and flowering plant species can promote linyphiid spiders, potentially by relaxing exploitative competition for shared prey.

Disentangling landscape and local drivers of ground-dwelling beetle community assembly in an urban ecosystem.

Community assembly is the process by which local communities are organized and maintained from the regional species pool. Understanding processes of insect assembly are of interest in "shrinking" cities where vacant land has become abundant as a result of protracted economic decline and population loss. Vacant land represents a viable conservation space for insects such as beetles that contribute to ecosystem services including pest suppression, decomposition, and nutrient cycling. However, the inherent heterogeneity of cities may pose challenges for beetle dispersal from source populations, while quality of the urban environment may constrain establishment. The objective of this study was to investigate the constraints to ground-dwelling beetle community assembly in vacant lots and pocket prairies of Cleveland, Ohio using a functional trait-based approach. Functional traits with a strong predictive capacity for ecological functions were measured on beetle species collected via pitfall traps. Assembly of beetle communities was primarily constrained by dispersal limitations to colonization. Over 93% of species found within treatments were capable of flight, and functional diversity of beetle communities was higher across all treatments than expected by chance. Once beetles colonized, successful establishment was influenced by heavy metal contamination and mowing frequency, with these disturbances shaping communities based on body size, antennae length, and origin. Colonization of dispersal-limited species could be facilitated by increasing connectivity among greenspaces in cities, while establishment could be enhanced by managing local environmental conditions. Understanding how insect communities are structured in urban ecosystems provides context for observed patterns of biodiversity, advances conservation efforts, and fosters ecosystem services.

Examining the distributional equity of urban tree canopy cover and ecosystem services across United States cities.

Examining the distributional equity of urban tree canopy cover (UTCC) has increasingly become an important interdisciplinary focus of ecologists and social scientists working within the field of environmental justice. However, while UTCC may serve as a useful proxy for the benefits provided by the urban forest, it is ultimately not a direct measure. In this study, we quantified the monetary value of multiple ecosystem services (ESD) provisioned by urban forests across nine U.S. cities. Next, we examined the distributional equity of UTCC and ESD using a number of commonly investigated socioeconomic variables. Based on trends in the literature, we predicted that UTCC and ESD would be positively associated with the variables median income and percent with an undergraduate degree and negatively associated with the variables percent minority, percent poverty, percent without a high school degree, percent renters, median year home built, and population density. We also predicted that there would be differences in the relationships between each response variable (UTCC and ESD) and the suite of socioeconomic predictor variables examined because of differences in how each response variable is derived. We utilized methods promoted within the environmental justice literature, including a multi-city comparative analysis, the incorporation of high-resolution social and environmental datasets, and the use of spatially explicit models. Patterns between the socioeconomic variables and UTCC and ESD did not consistently support our predictions, highlighting that inequities are generally not universal but rather context dependent. Our results also illustrated that although the variables UTCC and ESD had largely similar relationships with the predictor variables, differences did occur between them. Future distributional equity research should move beyond the use of proxies for environmental amenities when possible while making sure to consider that the use of ecosystem service estimates may result in different patterns with socioeconomic variables of interest. Based on our findings, we conclude that understanding and remedying the challenges associated with inequities requires an understanding of the local social-ecological system if larger sustainability goals are to be achieved.

A reference cytochrome c oxidase subunit I database curated for hierarchical classification of arthropod metabarcoding data.

Metabarcoding is a popular application which warrants continued methods optimization. To maximize barcoding inferences, hierarchy-based sequence classification methods are increasingly common. We present methods for the construction and curation of a database designed for hierarchical classification of a 157 bp barcoding region of the arthropod cytochrome c oxidase subunit I (COI) locus. We produced a comprehensive arthropod COI amplicon dataset including annotated arthropod COI sequences and COI sequences extracted from arthropod whole mitochondrion genomes, the latter of which provided the only source of representation for Zoraptera, Callipodida and Holothyrida. The database contains extracted sequences of the target amplicon from all major arthropod clades, including all insect orders, all arthropod classes and Onychophora, Tardigrada and Mollusca outgroups. During curation, we extracted the COI region of interest from approximately 81 percent of the input sequences, corresponding to 73 percent of the genus-level diversity found in the input data. Further, our analysis revealed a high degree of sequence redundancy within the NCBI nucleotide database, with a mean of approximately 11 sequence entries per species in the input data. The curated, low-redundancy database is included in the Metaxa2 sequence classification software (http://microbiology.se/software/metaxa2/). Using this database with the Metaxa2 classifier, we performed a cross-validation analysis to characterize the relationship between the Metaxa2 reliability score, an estimate of classification confidence, and classification error probability. We used this analysis to select a reliability score threshold which minimized error. We then estimated classification sensitivity, false discovery rate and overclassification, the propensity to classify sequences from taxa not represented in the reference database. Our work will help researchers design and evaluate classification databases and conduct metabarcoding on arthropods and alternate taxa.

Current Approaches and Clinician Attitudes to the Use of Cerebrospinal Fluid Biomarkers in Diagnostic Evaluation of Dementia in Europe.

BACKGROUND: BIOMARKAPD seeks to diminish the barriers associated with the clinical use of cerebrospinal fluid (CSF) biomarker analysis by reducing variation in CSF laboratory methodologies and generating consensus recommendations on their clinical interpretation and application for dementia diagnosis. OBJECTIVE: To examine the disparity in practitioner attitudes and clinical practice relating to the use of CSF biomarkers for dementia diagnosis across Europe. METHODS: Clinical dementia experts were surveyed on the prevalence of national consensus guidelines and analytical reimbursement across Europe, their biomarker platform preferences, lumbar puncture methodologies and application of reference values and cut-offs for CSF analysis. RESULTS: 74% of respondents (total n = 51) use CSF biomarkers in clinical practice and 69% perform lumbar punctures on an outpatient basis. Most use CSF biomarkers to diagnose atypical (84%) and early-onset cases of cognitive impairment (71%) and for the differential diagnosis of other dementias (69%). 82% state they are sufficiently informed about CSF biomarkers yet 61% report a lack of national consensus guidelines on their use for dementia diagnosis. 48% of countries represented do not reimburse clinical CSF analysis costs. 43% report using normal reference ranges derived from publications. CONCLUSION: Variations in attitude and practice relating to CSF biomarkers, widely recognised as barriers to their clinical acceptance, remain evident within and between countries across Europe, even in expert centres. These shortcomings must be addressed by developing consensus guidelines on CSF-related methodologies and their clinical application, to further their use for the diagnostic evaluation of dementia.

Influence of heavy metal contamination on urban natural enemies and biological control.

Urban agriculture is increasing worldwide. A history of contamination within urban landscapes may negatively impact the biota necessary for sustainable crop production, including arthropod natural enemies. This investigation revealed that heavy metal contamination can influence the composition of natural enemy communities and exposure can have reproductive, developmental, immunological and behavioral impacts on predators and parasitoids. Natural enemies exposed to heavy metals typically live shorter lives, take longer to develop and exhibit a reduced reproductive potential. Further, they may incur significant energy costs though the production of detoxification enzymes. This is a new and relatively unexplored area for biological control research, with important implications for our understanding of urban agricultural food web interactions.

A tale of two rain gardens: Barriers and bridges to adaptive management of urban stormwater in Cleveland, Ohio.

Green infrastructure installations such as rain gardens and bioswales are increasingly regarded as viable tools to mitigate stormwater runoff at the parcel level. The use of adaptive management to implement and monitor green infrastructure projects as experimental attempts to manage stormwater has not been adequately explored as a way to optimize green infrastructure performance or increase social and political acceptance. Efforts to improve stormwater management through green infrastructure suffer from the complexity of overlapping jurisdictional boundaries, as well as interacting social and political forces that dictate the flow, consumption, conservation and disposal of urban wastewater flows. Within this urban milieu, adaptive management-rigorous experimentation applied as policy-can inform new wastewater management techniques such as the implementation of green infrastructure projects. In this article, we present a narrative of scientists and practitioners working together to apply an adaptive management approach to green infrastructure implementation for stormwater management in Cleveland, Ohio. In Cleveland, contextual legal requirements and environmental factors created an opportunity for government researchers, stormwater managers and community organizers to engage in the development of two distinct sets of rain gardens, each borne of unique social, economic and environmental processes. In this article we analyze social and political barriers to applying adaptive management as a framework for implementing green infrastructure experiments as policy. We conclude with a series of lessons learned and a reflection on the prospects for adaptive management to facilitate green infrastructure implementation for improved stormwater management.

Effectiveness of early identification and electronic interventions for teens with risk factors for the development of heart disease and diabetes.

PURPOSE: Serum risk factors for the development of heart disease and diabetes are not routinely evaluated in teens. The intent of this study was to determine the prevalence of these risk factors in teens and evaluate the effectiveness of a two-part electronic education program (recurring electronic lifestyle education program [REEP]) on reducing risks. DATA SOURCES: Teens (n = 170) were recruited from one urban and one rural high school in the mid-Atlantic in 2014. Following baseline data collection in February, REEP was initiated and data collection repeated at 12 weeks. Data were analyzed and students sent a report with results and recommendations. CONCLUSIONS: One or more serum and/or physical risk factors were found in the majority of students with low vitamin D and elevated body mass index (BMI) being the most common. Correlations existed between elevated BMI and elevated diastolic blood pressure, low vitamin D, and low high-density lipoprotein. All but one risk factor (BMI) improved at 12 weeks. IMPLICATIONS FOR PRACTICE: The majority of teens had one or more physical and/or serum risk factors. Using multiple electronic methods to deliver healthy lifestyle recommendations helps lower these risks. Also, Blackboard, an electronic learning platform, was found to be an effective data management and communication center.