The effect of medroxyprogesterone acetate with or without eCG on conception rate of fat-tail ewes in out of breeding season.

Many protocols of estrous synchronization in ewes have been developed in the last few decades. The aim of this study was the effect of intravaginal progestogen, medroxyprogesterone acetate (MPA), sponges (a progesterone analogue) with or without equine chorionic gonadotropin (eCG) in out of breeding season of fat-tailed Iranian Shaal breed anestrus ewes to estimate the conception rate in suburb of Tehran-Iran. In total, 50 non-cyclic ewes out of 85 ewes with < 0.05 ng/ml progesterone (P4) and aged 2 to > 7 years old were selected for out of breeding season program in May-June 2018. They were distributed into two groups (control and treatment). The treatment group (no. 25) received 12 days a sponge containing 60 mg MPA and 500 IU eCG on the day of sponge removal. The control group (no. 25) received 12 days of sponge containing MPA only. Rams were introduced to the flock after removal of sponges in treatment group and concomitant in control group. They remained within the flock for 40 days. The ram to ewe ratio was 1 to 6. Transabdominal ultrasonography was performed for pregnancy diagnosis. The obtained data was analyzed using SPSS software version 16. The conception rate in treatment and control groups were differed significantly (P < 0.01). The conception rates in MPA and MPA+ eCG were 28% and 44%, respectively. Ewes (60%) with < 2 years old had the most conception rate. However, it was shown that use of vaginal sponges containing MPA and eCG in removal time of sponge can increase significantly the conception rate in out of breeding season fat-tail ewes Shaal breed.

Investigation of persistent infection of bovine viral diarrhea virus (BVDV) in Holstein dairy cows.

The aim of this study was to investigate the persistent infection (PI) of bovine viral diarrhea virus (BVDV) along with its coexistence between BVDV antibody titer and BVD virus in blood of Holstein dairy cows. Only large commercial farms (each contained < 1000-3000 unvaccinated cows) were included. There were 11 dairy cattle herds. They included nearly 20,000 dairy cows. Totally, 140 cows, > 3 months to almost 10 years old, were randomly sampled. Indirect enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect BVDV antibody and virus, respectively. The percent positivity (PP) < 14 and ≥ 14 values are interpreted negative and positive, respectively. Simultaneously, whole blood samples pooled in groups of 10 animals were used for molecular detection of BVDV. The results revealed that 138 (98.56%) out of 140 cows were positive for BVDV antibody, while the BVDV antigen was detected only in 2 (1.42%) cows, which were negative for BVDV antibody and so were considered as persistent infection (PI) cows. They were also retested 3 weeks apart. Since the results showed the strong coexistence between seropositivity and BVD virus, in the infected dairy cattle herds, the combination of simple ELISA and pooled whole blood RT-PCR strategy could be an achievable approach to detect PI animals.

Isolation and comparative investigation of vaginal mycoflora in feline population of urban and dairy cattle herds.

OBJECTIVE: The aim of this work was to identify the predominant fungal species present at vaginal site in different healthy cats including households, stray and cats in industrial dairy cattle herds. MATERIALS AND METHODS: Fungal isolates of vaginal fluids of 100 cats were collected using the sterilized cotton swabs from discharges of vagina. The isolates were identified according to their morphological characteristics and biochemical profile. Were included 34%, 33% and 33% households, stray cats and cats of industrial dairy cattle herds of Mashhad-Iran, respectively. They were short hair cats. RESULT: Vaginal fungi were isolated from 69% of feline population. Fungal isolates were obtained from vagina of 22%, 25% and 22% of households, stray cats and cats of industrial dairy cattle herds, respectively. There were no significant differences among the cats. The highest fungal agent was recovered in cats of 1-2 years old. Twenty two different isolates were recovered in this study. The most frequently recovered species samples were Penicillum spp. (11%) followed by Aspergillus section Nidulanti (4%). However, the vagina of healthy cats could be contaminated by 2-4 different fungal agents. CONCLUSION: It is concluded that fungal infections can occur in vaginal cavity of different healthy kinds of cats.

Intrauterine idiopathic amputation of the head of a porcine foetus.

An anencephalic full-term porcine foetus accompanied by a mummified head was submitted for examination. The neck almost entirely lacked skin and was covered by granulation tissue as were the exposed parts of the spine and spinal cord. The case represents a rare case of intrauterine amputation. A definitive cause could not be established because the placenta was not available. The most likely cause is strangulation of the neck. Such strangulation could be due to a defect of the allantoamnion with herniation of the foetal head or entanglement by amniotic constriction bands.