RESUMO
Seminal doses used for cervical and post-cervical artificial insemination (CAI and PCAI, respectively) vary in volume, the number of spermatozoa and packaging. The aim was to evaluate the outcomes when there was use of routine processing procedures for CAI- and PCAI-doses. Two different types of seminal doses were processed: 1) CAI: 2.7â¯×â¯109 sperm/80â¯ml; 2) PCAI: 1.5â¯×â¯109 sperm/45â¯ml. In Experiment 1, the cooling curve of seminal doses during processing occurred in two phases: 1st) At room temperature (23.4⯱â¯0.5⯰C) from 0 (just after packaging) to 120â¯min; 2nd) At refrigeration (15.7⯱â¯0.8⯰C) from 121-240â¯min. For the PCAI-doses, the time required to reach room temperature was 47â¯min compared to 107â¯min for CAI-doses (decreasing velocity of 0.093⯰C/min and 0.048⯰C/min, respectively). During refrigeration, for the PCAI-doses the time required to reach the desired preservation temperature was 20â¯min less than for CAI-doses (PCAI: 90â¯min, 0.074⯰C/min; CAI: 110â¯min, 0.066⯰C/min). In Experiment 2, sperm motility, kinetic parameters and acrosome damage for both types of doses were evaluated at 0, 24, 48 and 72â¯h of refrigeration. Also, morphology, pH, and osmolality were assessed at 0 and 72â¯h. Values for all these did not differ between CAI- and PCAI-doses. In conclusion, PCAI-doses took less time than CAI-doses to reach the desired temperature, but sperm quality was similar for CAI- and PCAI-doses during storage. Nevertheless, the different cooling curves should be taken into consideration for further investigation.