RESUMO
A flow cytometry-adapted fluorescent antibody to membrane antigen (FAMA) assay to detect IgG antibodies against varicella-zoster virus (VZV) was developed and tested in 62 serum samples, showing 90.32% accuracy obtained from a receiver operating characteristic (ROC) curve with a 0.9125 (95% confidence interval [CI], 0.829 to 1.00) area below the curve compared to the result with standard FAMA.
Assuntos
Anticorpos Antivirais/sangue , Citometria de Fluxo , Imunofluorescência , Herpesvirus Humano 3/imunologia , Imunidade , Antígenos de Superfície , Humanos , Imunoglobulina G/sangue , Curva ROC , Sensibilidade e EspecificidadeRESUMO
A new antitumor antibiotic, fredericamycin A (FCRC-A48, NSC-305263), has been isolated from a strain of Streptomyces griseus (FCRC-48). Based on its unique ultraviolet-visible spectrum, infrared spectrum, proton and carbon-13 nuclear magnetic resonance spectra and mass spectra, it is judged to be a novel acid-base indicator type of compound. Its production, isolation and physicochemical properties are discussed. The isolation, ultraviolet-visible spectrum and some biological properties of two minor components, fredericamycin B and fredericamycin C, are also described.
Assuntos
Antibióticos Antineoplásicos/isolamento & purificação , Antibióticos Antineoplásicos/biossíntese , Fenômenos Químicos , Físico-Química , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Fermentação , Isoquinolinas/isolamento & purificação , Compostos de Espiro/isolamento & purificação , Streptomyces griseus/metabolismoRESUMO
Isolation of a pentaene macrolide antibiotic (NSC-277813) from Streptomyces griseus (FCRC-21) fermentation broth is described. Using field desorption mass spectrometry and high resolution field desorption mass spectrometry on the intact and derivatized antibiotic and degradation products, the antibiotic was identified as fungichromin. The application of field desorption mass spectrometry in the identification of polyene antibiotics is discussed.
Assuntos
Antibióticos Antineoplásicos , Espectrometria de Massas , Antibióticos Antineoplásicos/análise , Antibióticos Antineoplásicos/isolamento & purificação , Macrolídeos , Métodos , Polienos/análise , Polienos/isolamento & purificação , Streptomyces griseus/análiseRESUMO
Three antibiotics possessing cytotoxic properties were isolated from a strain of Streptomyces griseus (FCRC-57). One was found to be identical with griseorhodin A. A second, FCRC-57-U, was found to be identical to griseorhodin C. FCRC-57-G is a new antibiotic structurally related to griseorhodins A and C, and is active against KB cells in vitro. The structure of this new antibiotic was determined using mass spectrometry, proton and carbon nuclear magnetic resonance spectroscopy and synthesis.