Assuntos
Acne Vulgar , Medicamentos sem Prescrição , Humanos , Acne Vulgar/tratamento farmacológico , Acne Vulgar/imunologia , Medicamentos sem Prescrição/uso terapêutico , Medicamentos sem Prescrição/efeitos adversos , Marketing , Fármacos Dermatológicos/uso terapêutico , Alérgenos/imunologia , Alérgenos/efeitos adversos , Hipersensibilidade a Drogas/imunologia , Hipersensibilidade a Drogas/diagnósticoAssuntos
Toxinas Botulínicas Tipo A/uso terapêutico , Carcinoma de Células Escamosas/cirurgia , Bochecha/cirurgia , Fístula/tratamento farmacológico , Doenças Parotídeas/tratamento farmacológico , Complicações Pós-Operatórias/tratamento farmacológico , Neoplasias Cutâneas/cirurgia , Idoso de 80 Anos ou mais , Carcinoma de Células Escamosas/patologia , Humanos , Masculino , Cirurgia de Mohs , Estadiamento de Neoplasias , Fármacos Neuromusculares/uso terapêutico , Neoplasias Cutâneas/patologiaRESUMO
The tethering together of sister chromatids by the cohesin complex ensures their accurate alignment and segregation during cell division. In vertebrates, sister chromatid cohesion requires the activity of the ESCO2 acetyltransferase, which modifies the Smc3 subunit of cohesin. It was shown recently that ESCO2 promotes cohesion through interaction with the MCM replicative helicase. However, ESCO2 does not significantly colocalize with the MCM complex, suggesting there are additional interactions important for ESCO2 function. Here we show that ESCO2 is recruited to replication factories, sites of DNA replication, through interaction with PCNA. We show that ESCO2 contains multiple PCNA-interaction motifs in its N terminus, each of which is essential to its ability to establish cohesion. We propose that multiple PCNA-interaction motifs embedded in a largely flexible and disordered region of the protein underlie the unique ability of ESCO2 to establish cohesion between sister chromatids precisely as they are born during DNA replication.
