New method of producing a more efficient coagulant for the treatment of water from seeds of moringa oleifera.

The coagulation is essential in raw water treatment. The most used coagulants are often of chemical origin and expensive and their use generates non-biodegradable residues. This is why innovative studies on the synthesis of natural coagulants based on plant extracts are very important. This study presents a new method of producing a coagulant for the treatment of water from seeds of moringa oleifera. The application of the new method is done in several steps: Harvesting moringa oleifera seeds, shelling, crushing the seeds, extracting oil from moringa oleifera powder, extracting salts from powder from moringa seeds oleifera, microfiltration with a 0.2 µm filter and lyophilization which gives the final product in dry form. We used the resulting product for coagulation of lake water with an initial turbidity of 40 NTU. This treatment reduced the turbidity of the water by almost 95 % when we used 0.7 mg of this coagulant per liter of water. Moringa has previously been used to produce coagulant but the peculiarity of this study is that it takes a very small amount of the synthesized product to effectively treat water. -The results of this research work have shown that the coagulant produced rom the seeds of Moringa oleifera can be used effectively for the treatment of surface water.-The residual turbidity obtained after the treatment of lake water with the coagulant produced was in accordance with the drinking water standard according to the World Health Organization (WHO) (less than 5 NTU).-The coagulant produced by this new method from moringa oleifera seed is an alternative to aluminum sulfate coagulant for water treatment.

UPLC-QToF-ESI-MS identification and anthelmintic activity of Mitragyna inermis (Willd.) Kuntze (Rubiaceae).

Medicinal plants attract the attention of many researchers to find natural and safe remedies for various resistant diseases. Leaves of Mitragyna inermis are widely used in traditional veterinary medicine for the treatment of gastrointestinal strongyles of small ruminants. The aim of the current study is to estimate the antioxidant, anthelmintic and the larval toxicity of the aqueous and hydroethanolic extracts of this plant in addition to the hexane, dichloromethane and ethanol fractions of the hydroethanolic extract. Investigation of the most active extract using Ultra Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight Electrospray Ionization Mass Spectrometry (UPLC-QToF-ESI-MS). Both plant extracts showed good antioxidant activity by scavenging the 2,2'-diphényl-1-picrylhydrazyl (DPPH) radical and reducing the ferric ion. Similarly, they were no-toxic to Artemia salina larvae (CL50 > 0.1 µg/mL). Also, they significantly reduced larval migration and motility of Haemonchus contortus adult worms (p < 0.001). The hexane, dichloromethane and ethanolic fractions of the hydroethanolic extract showed low activity compared to crude extracts except for the hexane fraction on H. contortus adult worms (p < 0.001) while it showed a poor result on larvae. It thus appears that the anthelmintic activity of the extract may be linked to the synergistic action of these compounds. The UPLC-QToF-ESI-MS analysis revealed the tentative identification of 15 compounds including 7 alkaloids. The results of the present study confirm the anthelmintic activity of M. inermis in traditional veterinary medicine.