RESUMO
Optimum storage conditions of cord blood-derived hematopoietic progenitor cells before isolation remain unknown. We therefore evaluated CD34+ cells isolated from cord blood units (n=57) within 1 h after collection and following storage for 24, 48 and 72 h at either room temperature (RT) or 4 degrees C. Isolated CD34+ cells were analyzed for their cell count, immunophenotype, apoptosis rate, clonogenicity and transmigration capacity in response to stroma-derived factor 1alpha using direct-paired comparisons (n=27). CD34+, CD133+ and CD45+ positivity after isolation remained the same under all conditions. After 24 h, CD34+ cell counts and numbers of CFU-GM colonies dropped regardless of the storage temperature. After 48 h, the number of CD34+ cells increased compared to 24 h, if the cord blood had been stored at RT resulting in almost three times more CD34+ cells than at 4 degrees C. These cells had a lower early apoptosis rate and formed four times more BFU-E than those stored at 4 degrees C with equivalent plating efficiencies. CD34+ cells kept at RT for 48 h had the highest transmigration capacities, which paralleled an increased CXCR-4 expression. Cord blood should be stored at RT before CD34+ isolation and a storage time for 48 h should be preferred to 24 h.
Assuntos
Preservação de Sangue/métodos , Separação Celular , Sangue Fetal/citologia , Células-Tronco Hematopoéticas/citologia , Antígenos CD34 , Apoptose , Preservação de Sangue/normas , Contagem de Células , Quimiotaxia , Células Clonais , Transplante de Células-Tronco Hematopoéticas , Imunofenotipagem , TemperaturaAssuntos
Anemia Falciforme/imunologia , Monócitos/imunologia , Fagocitose , Adolescente , Adulto , Anemia Falciforme/complicações , Infecções Bacterianas/etiologia , Candida , Citotoxicidade Imunológica , Suscetibilidade a Doenças , Feminino , Humanos , Síndromes de Imunodeficiência/etiologia , Masculino , Receptores de Complemento/fisiologia , Receptores de Complemento 3bAssuntos
Leucemia Linfoide/imunologia , Adolescente , Adulto , Linfócitos B/imunologia , Medula Óssea/patologia , Criança , Pré-Escolar , Feminino , Histocitoquímica , Humanos , Lactente , Leucemia Linfoide/sangue , Leucemia Linfoide/patologia , Linfócitos Nulos/imunologia , Masculino , Pessoa de Meia-Idade , Linfócitos T/imunologiaRESUMO
An increase of organelles occurs at the reticulocytary maturation stage, when compared with the erythroblastic one, in the peripheral blood of embryos and of patients with acquired hemolytic anemia. This increase is due to hemosome formation, in which possibly the final stage of hemoglobin biosynthesis may take place. In animals with posthemorrhagic anemia, biosynthesis does not occur and reticulocytes present only typical mitochondria. Based on these findings, as well as on reticulocyte basophilia, suggestions were done for the introduction of some modifications in the Harris & Kellermeyer16 1970 conception of the erythrocyte maturation.
