[Peptide mapping of the major proteins in 2 strains of influenza virus]. / Peptidnoe kartirovanie mazhornykh belkov v 2 shtammov virusa grippa.

The protein composition of two influenza A virus strains from different serological groups was studied. For preliminary separation of envelope and core proteins the virus was treated with nonionic detergent Triton X-100 solution followed by chromatography in ultragel AcA 34. After their separation by disk electrophoresis and treatment with trypsin they were labeled with tritium and peptide analysis was carried out. Differences in peptide maps between the appropriate light and heavy chains of hemagglutinins of the strains under study were demonstrated. Differences in the composition of nucleoprotein and matrix protein were insignificant.

[Complementarily addressed alkylation and cleavage of T7 phage DNA adjacent to the oligothymidylic sequences]. / Komplementarno adresovannoe alkilirovanie i fragmentatsiia DNK fara T17 vblizi oligotimidilovykh posledovatel'nostei.

Effect of temperature and reagent excess on the alkylation of the T7 phage DNA by an alkylating derivative of hexaadenylate that containes a modifying group on the 3'-terminus has been studied. Under condition of saturation at 20 degrees the reagent covalently binds adjacent to the 133 5'-PuN2--4Tn greater than or equal to 4 sequences in the T7 DNA; at 40 degrees the reagent alkylates 24 5'-PuN2Tn greater than or equal to 6 sequences. DNA containing specific apurinic sites has been prepared due to elimination of the alkylated purines. This DNA has been cleaved at the apurinic sites and specific DNA fragments have been obtained. Three types of DNA fragments are formed due to alkylation at 20 degrees: one fragment with 26,500 nucleotides in length, seven fragments with 4500 nucleotides in length and 103--111 fragments with an average length of 190 nucleotides. Alkylation at 40 degrees with following apurinization and cleavage yields 21--23 fragments with the lengths: 1--31,500; 1--19,000; 3--9500 and 17--19 with 1100 nucleotides in average.