Guideline for management of septic arthritis in native joints (SANJO).

This clinical guideline is intended for use by orthopedic surgeons and physicians who care for patients with possible or documented septic arthritis of a native joint (SANJO). It includes evidence and opinion-based recommendations for the diagnosis and management of patients with SANJO.

Evaluation of a syndromic panel polymerase chain reaction (spPCR) assay for the diagnosis of device-associated bone and joint infections (BJI).

OBJECTIVE: Pathogen detection is crucial for diagnosis and targeted therapy in implant-associated bone and joint infections (BJI). Culture-based microbiology regularly fails to identify causative pathogens. This study evaluated the diagnostic accuracy and clinical usefulness of a syndromic panel polymerase chain reaction (spPCR) assay targeting common BJI pathogens in tissue specimens from patients with implant-associated BJI. METHODS: Results obtained by spPCR assay and a 16S rDNA PCR were compared with results obtained from a standard of care (SOC) culture-based diagnostics, serving as a gold standard. In total, 126 specimens obtained from 73 patients were analyzed. RESULTS: The spPCR assay correctly identified 33/40 culture-positive samples (82.5 %) and was positive in 9/86 (10.5 %) culture-negative samples, resulting in an overall sensitivity of 84.6 % (95% confidence interval [CI] 68.79-93.59%) and specificity of 89.35% (95% CI 80.6-94.81%). The spPCR was more sensitive compared with the 16S rDNA PCR (37.5%). The spPCR identified pathogens in 7/51 (13.7%) SOC-negative patients. Re-evaluation of spPCR results in clinical context suggested their clinical significance. CONCLUSION: An spPCR assay targeting common pathogens causing implant-associated BJI may help to identify causative agents in culture-negative cases. As false-negative results are possible, spPCR assays appear as an add-on approach for pathogen detection in implant-associated BJI.

Functional outcome of septic arthritis after anterior cruciate ligament surgery.

PURPOSE: Septic arthritis is a rare complication after cruciate ligament surgery. The lack of conclusive evidence makes it difficult to obtain a consensus concerning the best treatment option. METHODS: From June 1993 to May 2010, 31 patients met the inclusion criteria for this prospective case series. The average age at ACL injury was 33.5 years. Treatment protocol was based on the grade of infection. Options included arthroscopic treatment for infections of Gaechter grades 1 and 2 or arthrotomy for infections of grades 3 and 4. Graft retention was decided based on the clinical findings. The setting was a specialized trauma hospital. Follow-up included International Knee Documentation Committee (IKDC) forms, Tegner score, and Lysholm scores at a mean of six years (71 months; range, 13-140) after treatment. RESULTS: In all cases, treatment of infection was successful; overall, a mean of 2.6 operations were required. In eight cases, it was possible to salvage the graft. The Tegner activity level before the knee injury was 6.5 points. At follow-up, the average score was 4.5 points. The postoperative subjective IKDC score averaged 63. The mean Lysholm score was 63.9. On clinical examination, a mean extension deficit of 2.5° and a mean maximum flexion of 121° were found. In the single-legged hop test, a mean capacity of 68% compared with the uninjured side was measured. CONCLUSION: The stage-adapted procedure gives reliable results for septic arthritis after ACL surgery. There were no recurrences of septic arthritis or bone infection. Early infection can be managed arthroscopically with satisfactory results. More advanced infections should be addressed with a more radical approach. In conclusion, functional outcome in most of the presented cases was only fair compared with results from ACL surgery not complicated by infection.

Surgical therapy of extensive knee joint empyema: mid-term results after two-stage versus one-stage procedures.

PURPOSE: Untreated knee joint empyema leads to rapid destruction of the joint and, thus far, has proven challenging to treat. This study presents data obtained after one- versus two-stage surgical approaches. Specifically, clinical outcome, complication rate, re-infection rate, duration of operation, and hospital stay are reported. METHODS: Between 2006 and 2010, 67 patients with extensive knee joint empyema underwent surgical therapy. Patients underwent either a two-stage surgical approach with open joint treatment for 7-10 days or a one-stage procedure with primary closure of the knee joint after radical debridement. RESULTS: The two-stage procedure was performed on 36 patients, whereas the one-stage procedure was performed on 31 patients. Infection duration prior to the index procedure ranged from 3 days to 55 months (two-stage: ~5 months, one-stage: ~2.4 months). The groups did not differ significantly in demographic group characteristics. Hospital stay and duration of operation were significantly (p < 0.01) reduced in the one-stage group compared to the two-stage group (31.1 ± 10.6 vs. 23.4 ± 14.4 days and 105 ± 32 vs. 68 ± 18 min, respectively). Functional outcome improved in both groups from the pre- to post-operative period, but did not differ significantly between groups. CONCLUSION: The one-stage open surgical approach produced similar results to the two-stage approach in the treatment of extensive knee joint empyemas. However, by waiving the open joint treatment concept, duration of hospital stay and operation time could be significantly reduced. LEVEL OF EVIDENCE: III.

Is non-union of tibial shaft fractures due to nonculturable bacterial pathogens? A clinical investigation using PCR and culture techniques.

BACKGROUND: Non-union continues to be one of the orthopedist's greatest challenges. Despite effective culture methods, the detection of low-grade infection in patients with non-union following tibial fracture still presents a challenge. We investigated whether "aseptic" tibial non-union can be the result of an unrecognized infection. METHODS: A total of 23 patients with non-union following tibial shaft fractures without clinical signs of infection were investigated. Intraoperative biopsy samples obtained from the non-union site were examined by means of routine culture methods and by polymerase chain reaction (PCR) for the detection of 16 S ribosomal RNA (rRNA). Control subjects included 12 patients with tibial shaft fractures. RESULTS: 23 patients (8 women and 15 men; mean age: 47.4 years) were included into this study. Preoperative C-reactive protein levels (mean: 20.8 mg/l) and WBC counts (mean: 8,359/µl) in the study group were not significantly higher than in the control group. None of the samples of non-union routine cultures yielded microorganism growth. Bacterial isolates were found by conventional culturing methods in only 1 case of an open fracture from the control group. In this case, PCR yielded negative results. 16 S rRNA was detected in tissue specimens from 2 patients (8.7%) with non-union. The analysis of these variable species-specific sequences enabled the identification of specific microorganisms (1x Methylobacterium species, 1x Staphylococcus species). Both PCR-positive patients were culture-negative. CONCLUSIONS: The combination of microbiological culture and broad-range PCR seems to substantially add to the number of microbiological diagnoses obtained and may improve the clinician's ability to tailor therapy to the individual patient's needs.

Medium-term results after total clavicle resection in cases of osteitis: a consecutive case series of five patients.

PURPOSE: Claviculectomy is a rare and poorly described event in the surgical therapy of diseases of the clavicle. We present a case series and functional results of patients who underwent total claviculectomy. METHODS: From 1995 to 2006 a total of 26 patients diagnosed with osteitis of the clavicle underwent surgery in our unit. Of these, five patients (all female) needed a total resection of the clavicle in order to ensure permanent healing from infection. The data collection was prospective. The data gathered preoperatively and at follow-up included clinical examinations, laboratory findings, radiographs and the Constant shoulder scores. The mean follow-up period was 7.5 months. RESULTS: The surgical concept described was able to eliminate infection in all cases studied within an average hospital stay of 13.4 days (8-18 days). Only one patient showed complications; suffering from chronic pain syndrome requiring surgical revision. After total resection of the clavicle four of five patients showed very good functional results. The average Constant score showed a significant increase from 82 before surgery to 95 at follow-up. CONCLUSIONS: The surgical technique described for total claviculectomy, along with the insertion of local antibiotic beads, was able to eliminate infection in every case. Good functional results and a low complication rates were observed. For good functional results and the permanent elimination of infection, adjacent joints have to be addressed, the periosteal tube should be preserved and early functional treatment is essential.

Surgical therapy of hip-joint empyema. Is the Girdlestone arthroplasty still up to date?

PURPOSE: Hip-joint empyema is a severe local infection that can cause general illness and, in the worst-case scenario, death by septic complications. For severe hip-joint infections, the Girdlestone arthroplasty has been an established treatment option for many decades. We describe functional midterm results after treatment of haematological and postoperative hip-joint infections. METHODS: From 2000 to 2010, 24 patients underwent surgical treatment for 27 hip-joint empyemas. The surgical procedures included radical debridement, implantation of local antibiotic beads and soft tissue management. Besides clinical, laboratory and imaging parameters, we analysed the Harris hip score (HHS). RESULTS: Twenty-three patients (26 hips) were followed up after a mean of 30 (3.1-126.8) months. The study group consists of 12 men and 11 women, with an average height of 1.71 m, weight of 84.7 kg and body mass index (BMI) of 28.6 kg/m(2). Hip-joint empyema was due to haematological septic spread in eight patients, surgery related in 12 hips and other causes in six cases. One patient died due to septic complications during the hospital stay. Intraoperative bacterial culture was positive in 50%, with Staphylococcus aureus as the most common organism (n = 11). Average hospital stay was 35 days. HHS significantly improved from 18.2 preoperatively to 47.8 at follow-up. Functional results were mainly poor, but pain relief increased significantly. The infection control rate was 96% with four (15%) complications. CONCLUSION: Resection arthroplasty by Girdlestone is able to control infection in most cases with an acceptable complication rate but poor functional results. In conclusion, the Girdlestone arthroplasty still is an essential surgical strategy for treating hip-joint empyema in cases in which functional outcome is of lesser priority.

Osteitis and Septic Arthritis after Tibial Head Fracture: Results of a Radical Treatment Regime.

OBJECTIVES: We report a series of 37 consecutive patients with both bone infection and septic arthritis of the knee following fracture of the proximal tibia. Aim of this study is to evaluate treatment results using a standardized treatment regime. The treatment regime consisted of an aggressive surgical approach to both the bone and the joint. In 61% the joint function was preserved, in the reminder an arthrodesis was required, there were no amputations. The difficulties in dealing with this condition are described as well as the clinical, radiological and occupational results. DESIGN/SETTING: Data collection was prospective. Data gathered at follow up included clinical examination, radiographs and the Rassmussen scoring system. Setting is a bone and joint infection and reconstruction unit of a tertiary referral hospital. PATIENTS/METHODS: Nine patients were female (24.3%). Mean age was 47.7 years (16.6-76.7 years). We classified fracture types according to Schatzker; ofwhich type VI fractures were the largest group with 13 cases. RESULTS: About 3.2 operations where required on average (1-7) for the treatment of infection. For 22 patients the joint function was salvaged. In the remaining cases an arthrodesis was required. CONCLUSIONS: The reported treatment regime gives reliable results for the treatment of combined bone and joint infection of the knee after fracture. In a large group of cases it was possible to salvage joint function. Comparison to other forms of treatment is not possible as there are no reported results so far.