RESUMO
The potential applications of plant extract and nanoparticles in antibacterial and antioxidant studies have garnered significant interest in recent times. Despite being utilized in Ethiopian traditional medicine, Verbascum sinaiticum (qetetina) constituents and its usage in nanoparticle synthesis remain relatively unexplored. This study explores the potential of the plant extract and its nanoparticles for antibacterial and antioxidant applications, with a focus on the leaf extracts and its silver nanoparticles. The leaf extract was analyzed using LC-MS and GC-MS and found to contain over 70 compounds, including glycosides, phenolic compounds, flavonoids, and fatty acids. The synthesized nanoparticles had a maximum absorbance of 408 nm, with a size range of 2-40 nm and showed a spherical shape. Using the agar well diffusion method, the extract and nanoparticles were evaluated against Gram-positive (Staphylococcus aureus ATCC 2592, Streptococcus agalactia ATCC12386) and Gram-negative bacteria (Acinetobacter baumannii ATCC19606, Pseudomonas aeruginosa ATCC27853) bacterial strains. In terms of antibacterial effects, both the silver nanoparticles and leaf extract displayed a greater impact on gram-positive bacterial strains over gram-negative bacterial strains. Additionally, the tests for lowest inhibitory and bactericidal concentration indicated similar outcomes. Notably, the silver nanoparticles exhibited greater antibacterial activity compared to the leaf extract alone. The DPPH (2, 2-diphenylpicrylhydrazyl) assay was conducted to investigate antioxidant activity. The results showed that the plant extract had an IC50 value of 143 µg/ml, while the synthesized nanoparticle had an IC50 value of 216 µg/ml, indicating that the plant extract had greater antioxidant activity than the synthesized silver nanoparticles.
