Evaluation of the effects of chitosan nanoparticles on polyhydroxy butyrate electrospun scaffolds for cartilage tissue engineering applications.

In this study, we synthesized and incorporated chitosan nanoparticles (Cs) into polyhydroxy butyrate (PHB) electrospun scaffolds for cartilage tissue engineering. The Cs nanoparticles were synthesized via an ionic gel interaction between Cs powder and tripolyphosphate (TPP). The mechanical properties, hydrophilicity, and fiber diameter of the PHB scaffolds with varying concentrations of Cs nanoparticles (1-5 wt%) were evaluated. The results of these evaluations showed that the scaffold containing 1 wt% Cs nanoparticles (P1Cs) was the optimum scaffold, with increased ultimate strength from 2.6 to 5.2 MPa and elongation at break from 5.31 % to 12.6 %. Crystallinity, degradation, and cell compatibility were also evaluated. The addition of Cs nanoparticles decreased crystallinity and accelerated hydrolytic degradation. MTT assay results showed that the proliferation of chondrocytes on the scaffold containing 1 wt% Cs nanoparticles were significantly higher than that on pure PHB after 7 days of cultivation. These findings suggest that the electrospun P1Cs scaffold has promising potential as a substrate for cartilage tissue engineering applications. This combination offers a promising approach for the fabrication of biomimetic scaffolds with enhanced mechanical properties, hydrophilicity, and cell compatibility for tissue engineering applications.

Evaluation of the effects of halloysite nanotube on polyhydroxybutyrate - chitosan electrospun scaffolds for cartilage tissue engineering applications.

Scaffolding method and material that mimic the extracellular matrix (ECM) of host tissue is an integral part of cartilage tissue engineering. This study aims to enhance the properties of electrospun scaffolds made of polyhydroxybutyrate (PHB) - Chitosan (Cs) by adding 1, 3, and 5 wt% halloysite nanotubes (HNT). The morphological, mechanical, and hydrophilicity evaluations expressed that the scaffold containing 3 wt% HNT exhibits the most appropriate features. The FTIR and Raman analysis confirmed hydrogen bond formation between the HNT and PHB-Cs blend. 3 wt% of HNT incorporation decreased the mean fibers' diameter from 965.189 to 745.16 nm and enhanced tensile strength by 169.4 %. By the addition of 3 wt% HNT, surface contact angle decreased from 61.45° ± 3.3 to 46.65 ± 1.8° and surface roughness increased from 684.69 to 747.62 nm. Our findings indicated that biodegradation had been slowed by incorporating HNT into the PHB-Cs matrix. Also, MTT test results demonstrated a significant increase in cell viability of chondrocytes on the PHB-Cs/3 wt% HNT (PC-3H) scaffold after 7 days of cell culture. Accordingly, the PC-3H scaffold can be considered a potential candidate for cartilage tissue engineering.