Colorimetric and gas-liquid chromatographic determination of atropine-hyoscyamine and hyoscine (scopolamine) in solanaceous plants.

Two colorimetric micromethods are described for the determination of atropine-hyoscyamine and hyoscine (scopolamine), using p-dimethylaminobenzaldehyde and citric acid-acetic anhydride as the color reagents. These methods are sensitive to 60-1200 and 10-360 mug alkaloid/10 ml. The colorimetric methods were also successfully applied after a preliminary thin layer chromatographic separation of the alkaloids. A gas-liquid chromatographic procedure was also developed, which yielded comparable results with the colorimetric methods.

Estimation of glycoalkaloids as solasodine in Solanum laciniatum.

Solasodine is isolated from Solanum laciniatum leaves by a rapid, one-step procedure which involves extraction and hydrolysis of the glycoalkaloids. The resulting steroidal aglycone is directly estimated by the formation of a colored complex with bromocresol green. The method is applicable to concentrations of 25-125 mug solasodine/7ml.