RESUMO
Sphingobium yanoikuyae B1 initiates the catabolism of biphenyl by adding dioxygen to the aromatic nucleus to form (+)-cis-(2R, 3S)-dihydroxy-1-phenylcyclohexa-4,6-diene. The present study focuses on the biphenyl 2,3-dioxygenase system, which catalyzes the dioxygenation reaction. This enzyme has been shown to have a broad substrate range, catalyzing the dioxygenation of not only biphenyl, but also three- and four-ring polycyclic aromatic hydrocarbons. Extracts prepared from biphenyl-grown B1 cells contained three protein components that were required for the oxidation of biphenyl. The genes encoding the three components (bphA4, bphA3 and bphA1f,A2f) were expressed in Escherichia coli. Biotransformations of biphenyl, naphthalene, phenanthrene, and benzo[a]pyrene as substrates using the recombinant E. coli strain resulted in the formation of the expected cis-dihydrodiol products previously shown to be produced by biphenyl-induced strain B1. The three protein components were purified to apparent homogeneity and characterized in detail. The reductase component (bphA4), designated reductase(BPH-B1), was a 43 kD monomer containing one mol FAD/mol reductase(BPH-B1). The ferredoxin component (bphA3), designated ferredoxin(BPH-B1), was a 12 kD monomer containing approximately 2 g-atoms each of iron and acid-labile sulfur. The oxygenase component (bphA1f,A2f), designated oxygenase(BPH-B1), was a 217 kD heterotrimer consisting of alpha and beta subunits (approximately 51 and 21 kD, respectively). The iron and acid-labile sulfur contents of oxygenase(BPH-B1) per alphabeta were 2.4 and 1.8 g-atom per mol, respectively. Reduced ferredoxin(BPH-B1) and oxygenase(BPH-B1) each gave EPR signals typical of Rieske [2Fe-2S] proteins. Crystals of reductase(BPH-B1), ferredoxin(BPH-B1) and oxygenase(BPH-B1 )diffracted to 2.5 A, 2.0 A and 1.75 A, respectively. The structures of the three proteins are currently being determined.
Assuntos
Proteínas de Bactérias/metabolismo , Compostos de Bifenilo/metabolismo , Dioxigenases/metabolismo , Sphingomonadaceae/enzimologia , Sequência de Aminoácidos , Proteínas de Bactérias/química , Proteínas de Bactérias/isolamento & purificação , Compostos de Bifenilo/química , Cristalização/métodos , Dioxigenases/química , Dioxigenases/isolamento & purificação , Espectroscopia de Ressonância de Spin Eletrônica , Eletroforese em Gel de Poliacrilamida , Ferredoxinas/química , Ferredoxinas/metabolismo , Estrutura Molecular , Oxigenases/química , Oxigenases/metabolismo , Sphingomonadaceae/metabolismoRESUMO
The protein components of the 2-nitrotoluene (2NT) and nitrobenzene dioxygenase enzyme systems from Acidovorax sp. strain JS42 and Comamonas sp. strain JS765, respectively, were purified and characterized. These enzymes catalyze the initial step in the degradation of 2-nitrotoluene and nitrobenzene. The identical shared reductase and ferredoxin components were monomers of 35 and 11.5 kDa, respectively. The reductase component contained 1.86 g-atoms iron, 2.01 g-atoms sulfur, and one molecule of flavin adenine dinucleotide per monomer. Spectral properties of the reductase indicated the presence of a plant-type [2Fe-2S] center and a flavin. The reductase catalyzed the reduction of cytochrome c, ferricyanide, and 2,6-dichlorophenol indophenol. The ferredoxin contained 2.20 g-atoms iron and 1.99 g-atoms sulfur per monomer and had spectral properties indicative of a Rieske [2Fe-2S] center. The ferredoxin component could be effectively replaced by the ferredoxin from the Pseudomonas sp. strain NCIB 9816-4 naphthalene dioxygenase system but not by that from the Burkholderia sp. strain LB400 biphenyl or Pseudomonas putida F1 toluene dioxygenase system. The oxygenases from the 2-nitrotoluene and nitrobenzene dioxygenase systems each had spectral properties indicating the presence of a Rieske [2Fe-2S] center, and the subunit composition of each oxygenase was an alpha(3)beta(3) hexamer. The apparent K(m) of 2-nitrotoluene dioxygenase for 2NT was 20 muM, and that for naphthalene was 121 muM. The specificity constants were 7.0 muM(-1) min(-1) for 2NT and 1.2 muM(-1) min(-1) for naphthalene, indicating that the enzyme is more efficient with 2NT as a substrate. Diffraction-quality crystals of the two oxygenases were obtained.
Assuntos
Comamonadaceae/enzimologia , Comamonas/enzimologia , Dioxigenases/metabolismo , Nitrobenzenos/metabolismo , Tolueno/análogos & derivados , Tolueno/metabolismo , Comamonadaceae/crescimento & desenvolvimento , Comamonas/crescimento & desenvolvimento , Cristalização , Dioxigenases/química , Dioxigenases/isolamento & purificação , Cinética , Relação Estrutura-AtividadeRESUMO
The importance of five amino acids at the active site of the multicomponent naphthalene dioxygenase (NDO) system was determined by generating site-directed mutations in various combinations. The substrate specificities of the mutant enzymes were tested with the substrates indole, indoline, 2-nitrotoluene (2NT), naphthalene, biphenyl, and phenanthrene. Transformation of these substrates measured the ability of the mutant enzymes to catalyze dioxygenation, monooxygenation, and desaturation reactions. In addition, the position of oxidation and the enantiomeric composition of products were characterized. All enzymes with up to three amino acid substitutions were able to catalyze dioxygenation reactions. A subset of these enzymes could also catalyze the monooxygenation of 2NT and desaturation of indoline. Single amino acid substitutions at positions 352 and 206 had the most profound effects on product formation. Of the single mutations made, only changes at position 352 affected the stereochemistry of naphthalene cis-dihydrodiol formed from naphthalene, but in the presence of the F352I mutation, changes at positions 206 and 295 also affected enantioselectivity. Major shifts in regioselectivity with biphenyl and phenanthrene resulted with several of the singly, doubly, and triply mutated enzymes. A new product not formed by the wild-type enzyme, phenanthrene cis-9,10-dihydrodiol, was formed as a major product from phenanthrene by enzymes with two (A206I/F352I) or three amino acid substitutions (A206I/F352I/H295I). The results indicate that a variety of amino acid substitutions are tolerated at the active site of NDO.
Assuntos
Hidrocarbonetos Aromáticos/metabolismo , Complexos Multienzimáticos/genética , Mutagênese Sítio-Dirigida , Oxigenases/genética , Tolueno/análogos & derivados , Sítios de Ligação , Compostos de Bifenilo/metabolismo , Western Blotting , Dioxigenases , Eletroforese em Gel de Poliacrilamida , Escherichia coli/enzimologia , Escherichia coli/genética , Indóis/metabolismo , Complexos Multienzimáticos/química , Complexos Multienzimáticos/metabolismo , Naftalenos/metabolismo , Oxigenases/química , Oxigenases/metabolismo , Fenantrenos/metabolismo , Conformação Proteica , Pseudomonas/enzimologia , Estereoisomerismo , Especificidade por Substrato , Tolueno/metabolismoRESUMO
Naphthalene 1,2-dioxygenase (NDOS) is a three-component enzyme that catalyzes cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene formation from naphthalene, O2, and NADH. We have determined the conditions for a single turnover of NDOS for the first time and studied the regulation of catalysis. As isolated, the alpha3beta3 oxygenase component (NDO) has up to three catalytic pairs of metal centers (one mononuclear Fe2+ and one diferric Rieske iron-sulfur cluster). This form of NDO is unreactive with O2. However, upon reduction of the Rieske cluster and exposure to naphthalene and O2, approximately 0.85 cis-diol product per occupied mononuclear iron site rapidly forms. Substrate binding is required for oxygen reactivity. Stopped-flow and chemical quench analyses indicate that the rate constant of the single turnover product-forming reaction significantly exceeds the NDOS turnover number. UV-visible and electron paramagnetic resonance spectroscopies show that during catalysis, one mononuclear iron and one Rieske cluster are oxidized per product formed, satisfying the two-electron reaction stoichiometry. The addition of oxidized or reduced NDOS ferredoxin component (NDF) increases both the product yield and rate of oxidation of formerly unreactive Rieske clusters. The results show that NDO alone catalyzes dioxygenase chemistry, whereas NDF appears to serve only an electron transport role, in this case redistributing electrons to competent active sites.
Assuntos
Complexos Multienzimáticos/metabolismo , Oxigênio/metabolismo , Oxigenases/metabolismo , Catálise , Dioxigenases , Espectroscopia de Ressonância de Spin Eletrônica , OxirreduçãoRESUMO
The naphthalene dioxygenase (NDO) system catalyzes the first step in the degradation of naphthalene by Pseudomonas sp. strain NCIB 9816-4. The enzyme has a broad substrate range and catalyzes several types of reactions including cis-dihydroxylation, monooxygenation, and desaturation. Substitution of valine or leucine at Phe-352 near the active site iron in the alpha subunit of NDO altered the stereochemistry of naphthalene cis-dihydrodiol formed from naphthalene and also changed the region of oxidation of biphenyl and phenanthrene. In this study, we replaced Phe-352 with glycine, alanine, isoleucine, threonine, tryptophan, and tyrosine and determined the activity with naphthalene, biphenyl, and phenanthrene as substrates. NDO variants F352W and F352Y were marginally active with all substrates tested. F352G and F352A had reduced but significant activity, and F352I, F352T, F352V, and F352L had nearly wild-type activities with respect to naphthalene oxidation. All active enzymes had altered regioselectivity with biphenyl and phenanthrene. In addition, the F352V and F352T variants formed the opposite enantiomer of biphenyl cis-3,4-dihydrodiol [77 and 60% (-)-(3S,4R), respectively] to that formed by wild-type NDO [>98% (+)-(3R,4S)]. The F352V mutant enzyme also formed the opposite enantiomer of phenanthrene cis-1,2-dihydrodiol from phenanthrene to that formed by biphenyl dioxygenase from Sphingomonas yanoikuyae B8/36. A recombinant Escherichia coli strain expressing the F352V variant of NDO and the enantioselective toluene cis-dihydrodiol dehydrogenase from Pseudomonas putida F1 was used to produce enantiomerically pure (-)-biphenyl cis-(3S,4R)-dihydrodiol and (-)-phenanthrene cis-(1S,2R)-dihydrodiol from biphenyl and phenanthrene, respectively.
Assuntos
Antracenos/metabolismo , Compostos de Bifenilo/metabolismo , Complexos Multienzimáticos/metabolismo , Oxigenases/metabolismo , Fenantrenos/metabolismo , Fenilalanina/metabolismo , Sphingomonas/enzimologia , Antracenos/química , Compostos de Bifenilo/química , Dioxigenases , Complexos Multienzimáticos/genética , Mutagênese Sítio-Dirigida , Oxigenases/genética , Fenantrenos/química , Fenilalanina/genética , Estereoisomerismo , Relação Estrutura-Atividade , Especificidade por SubstratoRESUMO
Aromatic hydrocarbon dioxygenases belong to a large family of Rieske non-heme iron oxygenases. The dioxygenases have a broad substrate specificity and catalyze enantiospecific reactions with a wide range of substrates. These characteristics make them attractive synthons for the production of industrially and medically important chiral chemicals and also provide essential information for the development of bioremediation technology.
Assuntos
Biotecnologia , Oxigenases/metabolismo , Biodegradação Ambiental , Hidrocarbonetos Aromáticos , Oxigenases/química , Oxigenases/classificação , Filogenia , Relação Estrutura-Atividade , Especificidade por Substrato , Terminologia como AssuntoRESUMO
Naphthalene 1,2 dioxygenase (NDO) displays characteristic UV-Vis spectra depending on the oxidation state of the Rieske center. Investigations on crystals of NDO grown for X-ray diffraction experiments showed spectra characteristic of the oxidized form. Crystals reduced in an anaerobic glovebox using sodium-dithionite showed a characteristic reduced spectrum. Spectra of crystals (cooled to 100 K) after being exposed to X-rays for data collection showed spectra corresponding to a reduced Rieske iron center, demonstrating the ability of X-rays to change the oxidation state of the Rieske iron-sulfur cluster in NDO.
Assuntos
Proteínas Ferro-Enxofre/efeitos da radiação , Ferro/efeitos da radiação , Complexos Multienzimáticos/efeitos da radiação , Oxigenases/efeitos da radiação , Enxofre/efeitos da radiação , Cristalização , Dioxigenases , Escherichia coli/enzimologia , Ferro/metabolismo , Proteínas Ferro-Enxofre/química , Proteínas Ferro-Enxofre/metabolismo , Microespectrofotometria , Complexos Multienzimáticos/química , Complexos Multienzimáticos/metabolismo , Oxirredução , Oxigenases/química , Oxigenases/metabolismo , Enxofre/metabolismo , Temperatura , Fatores de Tempo , Raios XRESUMO
The three-component naphthalene dioxygenase (NDO) enzyme system carries out the first step in the aerobic degradation of naphthalene by Pseudomonas sp. strain NCIB 9816-4. The three-dimensional structure of NDO revealed that several of the amino acids at the active site of the oxygenase are hydrophobic, which is consistent with the enzyme's preference for aromatic hydrocarbon substrates. Although NDO catalyzes cis-dihydroxylation of a wide range of substrates, it is highly regio- and enantioselective. Site-directed mutagenesis was used to determine the contributions of several active-site residues to these aspects of catalysis. Amino acid substitutions at Asn-201, Phe-202, Val-260, Trp-316, Thr-351, Trp-358, and Met-366 had little or no effect on product formation with naphthalene or biphenyl as substrates and had slight but significant effects on product formation from phenanthrene. Amino acid substitutions at Phe-352 resulted in the formation of cis-naphthalene dihydrodiol with altered stereochemistry [92 to 96% (+)-1R,2S], compared to the enantiomerically pure [>99% (+)-1R,2S] product formed by the wild-type enzyme. Substitutions at position 352 changed the site of oxidation of biphenyl and phenanthrene. Substitution of alanine for Asp-362, a ligand to the active-site iron, resulted in a completely inactive enzyme.
Assuntos
Substituição de Aminoácidos , Complexos Multienzimáticos/metabolismo , Mutagênese Sítio-Dirigida , Naftalenos/metabolismo , Oxigenases/metabolismo , Sítios de Ligação , Biodegradação Ambiental , Compostos de Bifenilo/metabolismo , Meios de Cultura , Dioxigenases , Escherichia coli/enzimologia , Escherichia coli/genética , Índigo Carmim , Indóis/metabolismo , Modelos Moleculares , Complexos Multienzimáticos/química , Complexos Multienzimáticos/genética , Oxigenases/química , Oxigenases/genética , Fenantrenos/metabolismo , Plasmídeos/genética , Especificidade por SubstratoRESUMO
The three-dimensional structure of the aromatic hydroxylating enzyme naphthalene dioxygenase (NDO) from Pseudomonas sp. NCIB 9816-4 was recently determined. The refinement of the structure together with cyclic averaging showed that in the active site of the enzyme there is electron density for a flat aromatic compound. This compound appears to be an indole adduct, which in Escherichia coli is derived from tryptophan present in the rich culture medium. An indole-dioxygen adduct has been built which fits the electron density convincingly. Support for this interpretation was obtained from crystals of the enzyme purified from cells grown in the absence of tryptophan which had an empty substrate pocket. These types of crystals were soaked in indole solutions and the position of indole in this complex was similar to the corresponding part in the modelled indole-oxygen adduct. This suggests that a peroxide bound to iron end-on attacks the substrate and forms this intermediate. The substrate position has implications for the substrate specificity of the enzyme. Docking studies with indole, naphthalene and biphenyl inside the substrate pocket of NDO suggest the presence of subpockets where the one close to the active site iron is reserved for the binding of the aromatic ring which is hydroxylated upon catalysis. The plausible location for the binding of dioxygen is between this pocket and the catalytic iron. This is in accordance with the enantiospecificity of the products.
Assuntos
Indóis/metabolismo , Complexos Multienzimáticos/química , Complexos Multienzimáticos/metabolismo , Oxigenases/química , Oxigenases/metabolismo , Pseudomonas/enzimologia , Sequência de Aminoácidos , Sítios de Ligação , Compostos de Bifenilo/química , Compostos de Bifenilo/metabolismo , Cristalização , Cristalografia por Raios X , Dioxigenases , Elétrons , Escherichia coli/genética , Escherichia coli/metabolismo , Hidroxilação , Indóis/química , Ferro/metabolismo , Modelos Moleculares , Dados de Sequência Molecular , Complexos Multienzimáticos/biossíntese , Complexos Multienzimáticos/genética , Naftalenos/química , Naftalenos/metabolismo , Oxigênio/química , Oxigênio/metabolismo , Oxigenases/biossíntese , Oxigenases/genética , Conformação Proteica , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Estereoisomerismo , Relação Estrutura-Atividade , Especificidade por Substrato , Triptofano/metabolismoRESUMO
The naphthalene dioxygenase enzyme system carries out the first step in the aerobic degradation of naphthalene by Pseudomonas sp. strain NCIB 9816-4. The crystal structure of naphthalene dioxygenase (B. Kauppi, K. Lee, E. Carredano, R. E. Parales, D. T. Gibson, H. Eklund, and S. Ramaswamy, Structure 6:571-586, 1998) indicates that aspartate 205 may provide the most direct route of electron transfer between the Rieske [2Fe-2S] center of one alpha subunit and mononuclear iron in the adjacent alpha subunit. In this study, we constructed four site-directed mutations that changed aspartate 205 to alanine, glutamate, asparagine, or glutamine to test whether this residue is essential for naphthalene dioxygenase activity. The mutant proteins were very inefficient in oxidizing naphthalene to cis-naphthalene dihydrodiol, and oxygen uptake in the presence of naphthalene was below detectable levels. The purified mutant protein with glutamine in place of aspartate 205 had identical spectral properties to wild-type naphthalene dioxygenase and was reduced by NADH in the presence of catalytic amounts of ferredoxinNAP and reductaseNAP. Benzene, an effective uncoupler of oxygen consumption in purified naphthalene dioxygenase, did not elicit oxygen uptake by the mutant protein. These results indicate that electron transfer from NADH to the Rieske center in the mutant oxygenase is intact, a finding consistent with the proposal that aspartate 205 is a necessary residue in the major pathway of electron transfer to mononuclear iron at the active site.
Assuntos
Complexos Multienzimáticos/química , Complexos Multienzimáticos/metabolismo , Oxigenases/química , Oxigenases/metabolismo , Ácido Aspártico/química , Sequência de Bases , Domínio Catalítico/genética , Primers do DNA/genética , Dioxigenases , Transporte de Elétrons , Escherichia coli/genética , Ferro/química , Modelos Moleculares , Complexos Multienzimáticos/genética , Mutagênese Sítio-Dirigida , Oxigenases/genética , Mutação Puntual , Conformação Proteica , Pseudomonas/enzimologia , Pseudomonas/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismoRESUMO
The oxygenase component of toluene dioxygenase from Pseudomonas putida F1 is an iron-sulfur protein (ISPTOL) consisting of alpha (TodC1) and beta (TodC2) subunits. Purified TodC1 gave absorbance and electron paramagnetic resonance spectra identical to those given by purified ISPTOL. TodC1 was reduced by NADH and catalytic amounts of ReductaseTOL and FerredoxinTOL. Reduced TodC1 did not oxidize toluene, and catalysis was strictly dependent on the presence of purified TodC2.
Assuntos
Oxigenases/química , Oxigenases/metabolismo , Pseudomonas putida/enzimologia , Catálise , Transporte de Elétrons , Ferredoxinas/metabolismo , Genes Bacterianos , Cinética , Oxirredução , Oxigenases/genética , Conformação Proteica , Pseudomonas putida/genéticaRESUMO
Beijerinckia sp strain B1 grows with biphenyl as its sole source of carbon and energy. A mutant, strain B8/36, oxidized biphenyl to cis-(2S,3R)-dihydroxy-l-phenylcyclohexa-4,6-diene (cis-biphenyl dihydrodiol). Strain B8/36 oxidized anthracene, phenanthrene, benz[a]anthracene and benzo[a]pyrene to cis-dihydrodiols. Other substrates oxidized to cis-dihydrodiols were dibenzofuran, dibenzothiophene and dibenzo-p-dioxin. Biphenyl dioxygenase activity was observed in cells of Beijerinckia B1 and B8/36 after growth in the presence of biphenyl, m-, p-xylene and salicylate. Recent studies have led to the reclassification of Beijerinckia B1 as Sphingomonas yanoikuyae strain B1. Subsequent biotransformation studies showed that S. yanoikuyae B8/36 oxidized chrysene to a bis-cis-diol with hydroxyl substituents at the 3,4- and 9,10-positions. Dihydronaphthalene was oxidized to cis-1,2-dihydroxy-1,2,3,4-tetrahydronaphthalene, naphthalene, cis-1,2-dihydroxy-1,2-dihydronaphthalene and 2-hydroxy-1,2-dihydronaphthalene. Anisole and phenetole were oxidized to phenol. Thus the S. yanoikuyae biphenyl dioxygenase catalyzes cis-dihydroxylation, benzylic monohydroxylation, desaturation and dealkylation reactions. To date, the genes encoding biphenyl dioxygenase have not been cloned. However, the nucleotide sequence of a S. yanoikuyaeB1 DNA fragment contains five different alpha subunits as determined by conserved amino acids coordinating iron in a Rieske [2Fe-2S] center and mononuclear iron at the catalytic site. The specific role of the different putative oxygenases in biotransformation reactions catalyzed by S. yanoikuyae is not known and presents an exciting challenge for future studies.
RESUMO
BACKGROUND: Pseudomonas sp. NCIB 9816-4 utilizes a multicomponent enzyme system to oxidize naphthalene to (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene. The enzyme component catalyzing this reaction, naphthalene 1,2-dioxygenase (NDO), belongs to a family of aromatic-ring-hydroxylating dioxygenases that oxidize aromatic hydrocarbons and related compounds to cis-arene diols. These enzymes utilize a mononuclear non-heme iron center to catalyze the addition of dioxygen to their respective substrates. The present study was conducted to provide essential structural information necessary for elucidating the mechanism of action of NDO. RESULTS: The three-dimensional structure of NDO has been determined at 2.25 A resolution. The molecule is an alpha 3 beta 3 hexamer. The alpha subunit has a beta-sheet domain that contains a Rieske [2Fe-2S] center and a catalytic domain that has a novel fold dominated by an antiparallel nine-stranded beta-pleated sheet against which helices pack. The active site contains a non-heme ferrous ion coordinated by His208, His213, Asp362 (bidentate) and a water molecule. Asn201 is positioned further away, 3.75 A, at the missing axial position of an octahedron. In the Rieske [2Fe-2S] center, one iron is coordinated by Cys81 and Cys101 and the other by His83 and His104. CONCLUSIONS: The domain structure and iron coordination of the Rieske domain is very similar to that of the cytochrome bc1 domain. The active-site iron center of one of the alpha subunits is directly connected by hydrogen bonds through a single amino acid, Asp205, to the Rieske [2Fe-2S] center in a neighboring alpha subunit. This is likely to be the main route for electron transfer.
Assuntos
Proteínas de Bactérias/química , Proteínas Ferro-Enxofre/química , Complexos Multienzimáticos/química , Oxigenases/química , Sequência de Aminoácidos , Proteínas de Bactérias/metabolismo , Sítios de Ligação , Cristalografia , Dioxigenases , Transporte de Elétrons , Complexo III da Cadeia de Transporte de Elétrons/química , Ferredoxinas/metabolismo , Hidroxilação , Proteínas Ferro-Enxofre/metabolismo , Modelos Moleculares , Dados de Sequência Molecular , Complexos Multienzimáticos/metabolismo , Naftalenos/metabolismo , Oxigenases/metabolismo , Conformação Proteica , Pseudomonas/enzimologiaRESUMO
Bacterial three-component dioxygenase systems consist of reductase and ferredoxin components which transfer electrons from NAD(P)H to a terminal oxygenase. In most cases, the oxygenase consists of two different subunits (alpha and beta). To assess the contributions of the alpha and beta subunits of the oxygenase to substrate specificity, hybrid dioxygenase enzymes were formed by coexpressing genes from two compatible plasmids in Escherichia coli. The activities of hybrid naphthalene and 2,4-dinitrotoluene dioxygenases containing four different beta subunits were tested with four substrates (indole, naphthalene, 2,4-dinitrotoluene, and 2-nitrotoluene). In the active hybrids, replacement of small subunits affected the rate of product formation but had no effect on the substrate range, regiospecificity, or enantiomeric purity of oxidation products with the substrates tested. These studies indicate that the small subunit of the oxygenase is essential for activity but does not play a major role in determining the specificity of these enzymes.
Assuntos
Proteínas Ferro-Enxofre/metabolismo , Complexos Multienzimáticos/metabolismo , Oxigenases/metabolismo , Burkholderia/enzimologia , Dinitrobenzenos/metabolismo , Dioxigenases , Escherichia coli/genética , Indóis/metabolismo , Proteínas Ferro-Enxofre/genética , Complexos Multienzimáticos/genética , Naftalenos/metabolismo , Naftóis/metabolismo , Oxirredução , Oxigenases/genética , Pseudomonas/enzimologia , Proteínas Recombinantes/metabolismo , Estereoisomerismo , Especificidade por Substrato , Tolueno/análogos & derivadosRESUMO
Biotransformations with recombinant Escherichia coli expressing the genes encoding 2-nitrotoluene 2,3-dioxygenase (2NTDO) from Pseudomonas sp. strain JS42 demonstrated that 2NTDO catalyzes the dihydroxylation and/or monohydroxylation of a wide range of aromatic compounds. Extremely high nucleotide and deduced amino acid sequence identity exists between the components from 2NTDO and the corresponding components from 2,4-dinitrotoluene dioxygenase (2,4-DNTDO) from Burkholderia sp. strain DNT (formerly Pseudomonas sp. strain DNT). However, comparisons of the substrates oxidized by these dioxygenases show that they differ in substrate specificity, regiospecificity, and the enantiomeric composition of their oxidation products. Hybrid dioxygenases were constructed with the genes encoding 2NTDO and 2,4-DNTDO. Biotransformation experiments with these hybrid dioxygenases showed that the C-terminal region of the large subunit of the oxygenase component (ISP alpha) was responsible for the enzyme specificity differences observed between 2NTDO and 2,4-DNTDO. The small subunit of the terminal oxygenase component (ISP beta) was shown to play no role in determining the specificities of these dioxygenases.
Assuntos
Oxigenases/metabolismo , Pseudomonas/enzimologia , Sequência de Aminoácidos , Sequência de Bases , Biodegradação Ambiental , Biotransformação , Dioxigenases , Escherichia coli/genética , Hidroxilação , Dados de Sequência Molecular , Complexos Multienzimáticos/química , Complexos Multienzimáticos/metabolismo , Naftalenos/metabolismo , Oxigenases/química , Oxigenases/genética , Pseudomonas/genética , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência , Estereoisomerismo , Especificidade por SubstratoRESUMO
A recombinant Escherichia coli strain which expresses naphthalene dioxygenase (NDO) from Pseudomonas sp. strain NCIB 9816-4 oxidized (S)-1-indanol to trans-(1S,3S)-indan-1,3-diol (95.5%) and (R)-3-hydroxy-1-indanone (4.5%). The same cells oxidized (R)-1-indanol to cis-1,3-indandiol (71%), (R)-3-hydroxy-1-indanone (18.2%), and cis-1,2,3-indantriol (10.8%). Purified NDO oxidized (S)-1-indenol to both syn- and anti-2,3-dihydroxy-1-indanol.
Assuntos
Indanos/metabolismo , Complexos Multienzimáticos/genética , Complexos Multienzimáticos/metabolismo , Oxigenases/genética , Oxigenases/metabolismo , Pseudomonas/genética , Dioxigenases , Escherichia coli/genética , Regulação Bacteriana da Expressão Gênica , Indanos/química , Isomerismo , Estrutura Molecular , Oxirredução , Proteínas Recombinantes/metabolismo , Recombinação GenéticaRESUMO
The ferredoxin component (ferredoxinBPH) of biphenyl 2,3-dioxygenase was purified to homogeneity from crude cell extract of Pseudomonas sp strain LB400 using ion exchange, hydrophobic interaction and gel filtration column chromatography. The protein was a monomer with a molecular weight of 15,000 and contained 2 gram-atoms each of iron and acid-labile sulfur. Ultraviolet-visible absorbance spectroscopy showed peaks at 325 nm and 460 nm with a broad shoulder around 575 nm. The spectrum was partially bleached in the visible region upon reduction by reductaseBPH with NADPH as the source of electrons. Electron paramagnetic resonance spectrometry showed no signals for the oxidized protein. Upon reduction with sodium dithionite, signals with gx = 1.82, gy = 1.92 and gz = 2.02 were detected. These results indicate that the protein contains a Rieske-type (2Fe-2S) iron-sulfur center. FerredoxinBPH was required for the oxidation of biphenyl by the terminal oxygenase component of the enzyme and is probably involved in the transfer of reducing equivalents from reductaseBPH to the terminal oxygenase during catalysis.
Assuntos
Ferredoxinas/isolamento & purificação , Proteínas Ferro-Enxofre , Oxigenases/análise , Pseudomonas/enzimologia , Compostos de Bifenilo/metabolismo , Ferredoxinas/metabolismoRESUMO
Comamonas sp strain JS765 utilizes nitrobenzene as a carbon and nitrogen source. The initial attack on nitrobenzene is carried out by nitrobenzene 1,2-dioxygenase, which converts nitrobenzene to an unstable nitrohydrodiol that spontaneously decomposes to form catechol and nitrite. Catechol is then degraded via a meta cleavage pathway. We now report the cloning of a DNA fragment carrying a catechol 2,3-dioxygenase gene from JS765. Nucleotide sequence analysis revealed three open reading frames (ORFs) predicted to encode proteins of 33.6, 13.0, and 35.0 kDa. Homology searches of the deduced amino acid sequences of three proteins suggested that ORF1 encodes a LysR-type transcriptional regulator, ORF2 encodes a XylT-type ferredoxin, and ORF3 encodes a catechol 2,3-dioxygenase. The putative regulatory gene, designated cdoR, is divergently transcribed from the ferredoxin and catechol dioxygenase genes, cdoT and cdoE, respectively. The catechol 2,3-dioxygenase is most similar in amino acid sequence to the 1.2.C subfamily of extradiol dioxygenases which include 3-methylcatechol 2,3-dioxygenase from the aniline- and toluidine-degrading Pseudomonas putida UCC2, TbuE from the toluene monooxygenase pathway of Pseudomonas pickettii PKO1 and catechol 2,3-dioxygenase II from the TOL plasmid pWW15. The substrate range of the catechol 2,3-dioxygenase produced by the recombinant E. coli strains was very similar to that of the enzyme present in nitrobenzene-grown JS765, suggesting that we have cloned the catechol 2,3-dioxygenase gene required for nitrobenzene degradation.
Assuntos
Dioxigenases , Bacilos e Cocos Aeróbios Gram-Negativos/enzimologia , Nitrobenzenos/metabolismo , Oxigenases/genética , Sequência de Aminoácidos , Sequência de Bases , Biodegradação Ambiental , Catecol 2,3-Dioxigenase , Clonagem Molecular , Dados de Sequência MolecularRESUMO
A new procedure was developed for the purification of the terminal oxygenase component (ISPNAP) of naphthalene dioxygenase. From a five liter culture of Escherichia coli JM109(DE3)(pDTG121), 91 mg of pure protein were obtained with a specific activity of 2.48 mumol/ min/mg protein. ISPNAP was crystallized in the rhombohedral space group R32 with cell dimensions of a = b = 179.2 A; c = 322.5 A in the hexagonal setting. The crystals are brown, indicating the presence of an intact Rieske iron-sulfur center. Problems with non-isomorphism between native data sets necessitated the preparation of a selenomethionine-substituted protein. Complete replacement of methionine with selenomethionine was achieved and the purified protein had a specific activity almost identical to native ISPNAP. Crystals from this preparation belong to the same space group and have similar cell dimensions to native ISPNAP.