Influence of Pichia pastoris X-33 produced in industrial residues on productive performance, egg quality, immunity, and intestinal morphometry in quails.

This study was conducted in quails to evaluate the probiotic potential of Pichia pastoris X-33, cultivated in parboiled rice effluent supplemented with biodiesel glycerol or in standard medium Yeast Extract-Peptone-Dextrose (YPD). Forty-days-old female quails were divided into three treatments: T1 (Control) received a basal diet without P. pastoris; T2 (Pichia Effluent) received a basal diet supplemented with P. pastoris grown in parboiled rice effluent and biodiesel glycerol, and T3 (Pichia YPD) received a basal diet supplemented with P. pastoris produced in YPD. The birds were vaccinated against Newcastle Disease (NDV), Avian Infectious Bronchitis (IBV), and Gumboro Disease on days 1 and 28. The following parameters were analyzed: performance, egg quality, humoral immune response to the vaccines, organ weight, and intestinal morphometry. P. pastoris grown in YPD increased egg weight (p < 0.05). The lowest liver weight on day 14 was obtained in Pichia Effluent, whereas both P. pastoris supplemented groups had the lowest duodenum weights on day 14. Besides that, livers and duodenums presented no morphological changes in any of the three treatments. Supplementation of P. pastoris modulated the immune system of the birds, increasing anti-IBV, anti-NDV, and anti-Gumboro antibodies levels compared to the Control (p < 0.05). In conclusion, quail's immune response was improved by Pichia pastoris X-33, either it was grown in YPD or industrial residues, and the egg weight increased with Pichia pastoris X-33 grown in YPD, thereby demonstrating to be a promising probiotic for poultry.

Bioremediation and biomass production from the cultivation of probiotic Saccharomyces boulardii in parboiled rice effluent.

The parboilization of rice generates 2 L of effluent per kilogram of processed grain. Several methodologies have previously been tested with the aim of reducing the environmental impact of this effluent. The objective of this study was to evaluate the bioremediation of parboiled rice effluent supplemented with sucrose or residual glycerol from the biodiesel during the cultivation of the Saccharomyces boulardii probiotic. In the first stage of the experiment, cultures were grown in orbital shaker, and five media compositions were evaluated: 1) parboiled rice effluent; 2) effluent supplemented with 1% sucrose; 3) effluent supplemented with 3% sucrose; 4) effluent supplemented with 15 g.L-1 of biodiesel glycerol and 5) standard yeast culture medium (YM). The addition of 1% of sucrose generated the most promising results in terms of cell viability, removal of nitrogen, phosphorus and chemical oxygen demand (COD). From these results, four independent cultures were grown in a bioreactor using effluent +1% of sucrose as the medium. This assays generated a mean of 3.8 g.L-1 of biomass, 1.8 × 1011 CFU.L-1, and removal of 74% of COD and 78% of phosphorus. Therefore, the cultivation of Saccharomyces boulardii in parboiled rice effluent supplemented with 1% sucrose may represent a viable method by which the environmental impact of this effluent can be reduced while simultaneously producing probiotic culture for use in animal production.

Probiotic effect of Pichia pastoris X-33 produced in parboiled rice effluent and YPD medium on broiler chickens.

In a previous paper we showed that the yeast Pichia pastoris X-33 grown in parboiled rice effluent supplemented with glycerol byproduct from the biodiesel industry improved the quality of the effluent. In this paper we show the validation of this yeast (PPE) as probiotic for broilers. Its effect on feed efficiency and immunomodulation was compared with the same yeast grown in yeast peptone dextrose medium (PPY), with Saccharomyces boulardii (SBY) and with the controls fed unsupplemented feed (CON). One-day-old female chicks were vaccinated against infectious bursal disease (IBD) and the titers of anti-IBD antibodies were measured by ELISA. PPE group had the highest mean titres on days 14 and 28 (p<0,05), and at 28 days, 64% of the animals showed seroconvertion. The PPE group also showed the best weight gains at 42 days of age, that, on days 7, 14 and 21 were 19%, 15%, and 8.7% higher, respectively, than the control group. The best feed conversion, 8.2% higher than the control group, was obtained by PPY at 42 days. Histopathological studies did not detect any undesirable effects in the supplemented animals. We concluded that Pichia pastoris X-33 when grown in effluents of the rice parboiling industry supplemented with glycerol byproduct from the biodiesel has probiotic properties for poultry.

Bioremediation of parboiled rice effluent supplemented with biodiesel-derived glycerol using Pichia pastoris X-33.

This paper describes the use of Pichia pastoris X-33 as a bioremediator to reduce the chemical oxygen demand (COD), total Kjeldahl nitrogen (TKN), and phosphorus (P-PO(4) (3-)), after culture in parboiled rice effluent supplemented with p.a. glycerol or a glycerol by-product of the biodiesel industry. The greatest reduction in the COD (55%), TKN (45%), and P-PO(4) (3-) (52%) of the effluent was observed in cultures of P. pastoris X-33 supplemented with 15 g.L(-1) of biodiesel-derived glycerol. Furthermore, the overall biomass yield was 2.1 g.L(-1). These data suggest that biodiesel-derived glycerol is an efficient carbon source for the bioremediation of parboiled rice effluent and biomass production.