RESUMO
Parasitoids of dried-fruit insects were surveyed at a culled fig warehouse in Fresno, CA. Three parasitoids of pyralid larvae were found: Habrobracon hebetor (Say), Venturia canescens (Gravenhorst), and at least one species in the genus Goniozus Förster. Two parasitoids of pyralid pupae also were noted: Mesostenus gracilis (Cresson) and a new species of Psilochalcis Keifer. The latter is a new host association. Several beetle parasitoids were present, including Anisopteromalus calandrae (Howard), three species of Cephalonomia Westwood, Laelius centratus (Say), and Cerchysiella utilis Noyes. C. utilis, a parasitoid of driedfruit beetle, Carpophilus hemipterus (L.), is a new record for California. Most activity by parasitoids (detected by yellow flight traps) occurred directly above the fig mass. Pyralid parasitoids exhibited two peaks of seasonal activity; one in late summer through early fall, shortly after new figs were brought into the warehouse, and one in the spring. H. hebetor generally attacked older host larvae, whereas V. canescens equally attacked older and younger larvae, indicating that these two parasitoids may coexist by exploiting different portions of the host population. H. hebetor was active throughout the winter, suggesting that winter release of H. hebetor could be used to control diapausing pyralid populations in dried fruit and nut storage areas.
Assuntos
Frutas , Insetos , Animais , Insetos/parasitologia , Estações do AnoRESUMO
PURPOSE: To define the inductive pathways leading to rat tear IgA antibody responses. METHODS: Fluoresceinated dinitrophenylated bovine serum albumin was encapsulated in poly(lactide-co-glycolide) microparticles and was administered by intranasal, ocular topical, or gastrointestinal routes. Histologic methods were used to determine the microparticles' ability to access tissues associated with mucosal inductive pathways. Rats were immunized with microencapsulated antigen by intranasal or ocular topical routes. Tear IgA and serum IgG antibody concentrations were assessed by radioimmunoassay. The frequency of antibody-secreting cells in tissues, postulated to function in tear IgA induction, was measured by enzyme-linked immunospot assay. RESULTS: Although uptake of microencapsulated antigen was greatest at the site of delivery, ocular topical administration resulted in antigen uptake in the conjunctiva and in nasal-associated lymphoid tissue. Intranasal immunization resulted in earlier and significantly higher tear IgA and serum IgG antibody responses and in higher frequencies of antibody-secreting cells in corresponding draining cervical lymph nodes and lacrimal glands than did ocular topical immunization. CONCLUSIONS: Nasal-associated lymphoid tissue functions as a primary inductive site for tear IgA antibody responses by contributing triggered IgA-committed B cells to the lacrimal gland.
Assuntos
Células Produtoras de Anticorpos/imunologia , Dinitrofenóis/imunologia , Imunoglobulina A Secretora/biossíntese , Soroalbumina Bovina/imunologia , Lágrimas/imunologia , Administração Intranasal , Administração Tópica , Animais , Formação de Anticorpos , Materiais Biocompatíveis , Dinitrofenóis/administração & dosagem , Ensaio de Imunoadsorção Enzimática , Feminino , Imunidade nas Mucosas , Imunoglobulina G/análise , Intubação Gastrointestinal , Ácido Láctico , Tecido Linfoide/imunologia , Microesferas , Ácido Poliglicólico , Copolímero de Ácido Poliláctico e Ácido Poliglicólico , Polímeros , Radioimunoensaio , Ratos , Ratos Endogâmicos F344 , Soroalbumina Bovina/administração & dosagemRESUMO
A synthetic peptide E474 SFAVATTAL, derived from the sequence of mouse mammary tumor virus envelope protein, was previously shown to bind class I MHC Kd. Immunization of BALB/c mice with E474 in 50% incomplete Freund's adjuvant (IFA) followed by in vitro stimulation of immune cells with E474-coated antigen-presenting cells resulted in peptide-specific cytotoxic T lymphocytes (CTL). Furthermore, anti-E474 CTL lysed mammary tumor cell lines D2F2 and D2A1, derived from a spontaneous tumor that arose in BALB/c pre-neoplastic hyperplastic alveolar nodule (HAN) D2 line. Expression of Kd by D2A1 and D2F2 cells was verified by flow cytometry, and lysis of D2 tumor cells was blocked by monoclonal antibody 31-34-S, which interacted with the peptide-binding region of Kd, supporting the recognition of E474 in Kd by anti-E474 CTL. Immunization of BALB/c mice with E474 before D2F2 tumor challenge resulted in reduced tumor growth. Therefore, E474 is naturally processed and presented by these tumor cells and can induce anti-tumor immunity.
Assuntos
Neoplasias Mamárias Experimentais/imunologia , Vírus do Tumor Mamário do Camundongo/química , Oligopeptídeos/imunologia , Peptídeos/imunologia , Linfócitos T Citotóxicos/imunologia , Proteínas do Envelope Viral/imunologia , Sequência de Aminoácidos , Animais , Antígenos de Neoplasias/biossíntese , Antígenos de Neoplasias/imunologia , Antígenos de Neoplasias/farmacologia , Divisão Celular/efeitos dos fármacos , Antígenos de Histocompatibilidade Classe I/biossíntese , Antígenos de Histocompatibilidade Classe I/imunologia , Antígenos de Histocompatibilidade Classe I/farmacologia , Ativação Linfocitária/efeitos dos fármacos , Neoplasias Mamárias Experimentais/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Oligopeptídeos/biossíntese , Oligopeptídeos/farmacologia , Biossíntese Peptídica , Peptídeos/farmacologia , Linfócitos T Citotóxicos/efeitos dos fármacos , Células Tumorais Cultivadas , Proteínas do Envelope Viral/biossíntese , Proteínas do Envelope Viral/farmacologiaRESUMO
Mouse mammary tumor virus MMTV (C4) encodes a V beta 2-specific superantigen. In V beta 2 transgenic (TG2) mice more than 98% of peripheral T cells express V beta 2. Infection of Tg2 mice with MMTV (C4) at birth through their mothers' milk or at 6-8 weeks of age by intravenous injection resulted in massive deletion of peripheral CD4+ T cells and suppressed thymopoiesis. The number of peripheral CD8+ T cells was not affected in neonatally infected mice. In older mice injected with MMTV (C4), splenic CD8+ T cells were significantly elevated. Suppressed thymopoiesis was observed in both neonatally infected and older mice injected with MMTV (C4). Thymocytes which expressed high level CD3 or V beta 2 were deleted. To determine if T cells or thymocytes were deleted through apoptosis, DNA fragmentation was examined by flow cytometry and diphenylamine (DPA) binding assay. Approximately 31% of CD4+ T cells from MMTV (C4)-infected Tg2 mice as compared to 6% from normal Tg2 mice contained fragmented nuclear DNA by flow-cytometric analysis. The DPA binding assay showed significantly increased total soluble DNA in lymph node cells and thymocytes from MMTV (C4)-infected mice. The kinetics of T cell and thymocyte apoptosis correspond to their deletion, supporting apoptosis as the mechanism of T cell and thymocyte deletion. CD4+ T cell and thymocyte deletion by MMTV (C4) in Tg2 mice provides a sensitive system for the analysis of retrovirus superantigen-induced apoptosis.
Assuntos
Linfócitos T CD4-Positivos/patologia , Vírus do Tumor Mamário do Camundongo/patogenicidade , Timo/patologia , Infecções Tumorais por Vírus/patologia , Animais , Apoptose , Sequência de Bases , Dano ao DNA , Primers do DNA/química , Feminino , Rearranjo Gênico da Cadeia beta dos Receptores de Antígenos dos Linfócitos T , Hematopoese , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Transgênicos , Dados de Sequência Molecular , Receptores de Antígenos de Linfócitos T alfa-beta/genética , Infecções Tumorais por Vírus/imunologiaRESUMO
Most peptides with putative MHC I restricted sequence motifs do not bind to the corresponding MHC I nor induce cytolytic T cells. There exist additional constraints which limit peptide binding and immunogenicity. To identify immunogenic peptides in novel protein sequences, it will be necessary to first evaluate peptide binding to MHC I. In this study, a soluble single chain fusion protein SC-Kd was used to evaluate potential Kd binding peptides from the sequences of mouse mammary tumor virus gag and env proteins. A total of 27 peptides were identified which displayed the reported Kd restricted motif. Of the 27 peptides, six demonstrated strong to moderate binding to SC-Kd. The strongest binding peptides expressed tyrosine or phenylalanine at position 2 and leucine at the C-terminus. The capability of MMTV peptides to induce CTL corresponds to their SC-Kd binding activity. Of the six peptides that demonstrated moderate to strong binding, five induced CTL in BALB/c mice. These peptides induced CTL after 1-3 in vivo immunizations followed by 5 day in vitro stimulation. Furthermore, a single in vitro stimulation of naive lymphocytes with strong-binding G425 was sufficient to induce significant CTL activity. Weak or non-binding peptides did not induce CTL. Therefore, peptide binding to SC-Kd is a predictive indicator of CTL inducing activity.
Assuntos
Antígenos H-2/imunologia , Fragmentos de Peptídeos/imunologia , Ligação Proteica/imunologia , Linfócitos T Citotóxicos/imunologia , Sequência de Aminoácidos , Animais , Testes Imunológicos de Citotoxicidade , Citometria de Fluxo , Imunização/métodos , Ativação Linfocitária/imunologia , Vírus do Tumor Mamário do Camundongo/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Proteínas Recombinantes de Fusão/imunologia , Baço/citologia , Baço/imunologia , Células Tumorais CultivadasRESUMO
There is a large body of literature on immune reactivity to mouse mammary tumor virus (MMTV) during mammary tumor progression. It is a general consensus that MMTV is antigenic and elicits cell-mediated and humoral immune responses. In addition, activation of non-specific inflammatory cells has been observed in various mouse preneoplastic and neoplastic mammary lesions. How these non-conventionally activated inflammatory cells affect tumor progression is a subject of debate. The discovery of MMTV associated superantigens solved certain long standing mysteries and may provide new insight into the cause of these unusual host responses. In this article, we will discuss how superantigen associated secondary immune responses may contribute to mammary tumor progression and the possible impact superantigen may have on specific anti-MMTV reactivity.