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1.
Adv Sci (Weinh) ; 9(10): e2103827, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35137562

RESUMO

Parkinson's disease (PD) evolves over an extended and variable period in humans; years prior to the onset of classical motor symptoms, sleep and biological rhythm disorders develop, significantly impacting the quality-of-life of patients. Circadian-rhythm disorders are accompanied by mild cognitive deficits that progressively worsen with disease progression and can constitute a severe burden for patients at later stages. The gold-standard 6-methyl-1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin (MPTP) macaque model of PD recapitulates the progression of motor and nonmotor symptoms over contracted periods of time. Here, this multidisciplinary/multiparametric study follows, in five animals, the steady progression of motor and nonmotor symptoms and describes their reversal following grafts of neural precursors in diverse functional domains of the basal ganglia. Results show unprecedented recovery from cognitive symptoms in addition to a strong clinical motor recuperation. Both motor and cognitive recovery and partial circadian rhythm recovery correlate with the degree of graft integration, and in a subset of animals, with in vivo levels of striatal dopaminergic innervation and function. The present study provides empirical evidence that integration of neural precursors following transplantation efficiently restores function at multiple levels in parkinsonian nonhuman primates and, given interindividuality of disease progression and recovery, underlines the importance of longitudinal multidisciplinary assessments in view of clinical translation.


Assuntos
Disfunção Cognitiva , Doença de Parkinson , Animais , Disfunção Cognitiva/etiologia , Dopamina , Humanos , Estudos Longitudinais , Macaca
2.
Front Cell Dev Biol ; 8: 588814, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33178700

RESUMO

The orderly radial migration of cortical neurons from their birthplace in the germinal zones to their final destination in the cortical plate is a prerequisite for the functional assembly of microcircuits in the neocortex. Rodent and primate corticogenesis differ both quantitatively and qualitatively, particularly with respect to the generation of neurons of the supragranular layers. Marked area differences in the outer subventricular zone progenitor cell density impact the radial glia scaffold compactness which is likely to induce area differences in radial migration strategy. Here, we describe specific features of radial migration in the non-human primate, including the absence of the premigratory multipolar stage found in rodents. Ex vivo approaches in the embryonic macaque monkey visual cortex, show that migrating neurons destined for supragranular and infragranular layers exhibit significant differences in morphology and velocity. Migrating neurons destined for the supragranular layers show a more complex bipolar morphology and higher motility rates than do infragranular neurons. There are area differences in the gross morphology and membrane growth behavior of the tip of the leading process. In the subplate compartment migrating neurons destined for the supragranular layers of presumptive area 17 exhibit radial constrained trajectories and leading processes with filopodia, which contrast with the meandering trajectories and leading processes capped by lamellipodia observed in the migrating neurons destined for presumptive area 18. Together these results present evidence that migrating neurons may exhibit autonomy and in addition show marked area-specific differences. We hypothesize that the low motility and high radial trajectory of area 17 migrating neurons contribute to the unique structural features of this area.

3.
Cereb Cortex ; 30(2): 656-671, 2020 03 21.
Artigo em Inglês | MEDLINE | ID: mdl-31343065

RESUMO

Perturbation of the developmental refinement of the corticospinal (CS) pathway leads to motor disorders. While non-primate developmental refinement is well documented, in primates invasive investigations of the developing CS pathway have been confined to neonatal and postnatal stages when refinement is relatively modest. Here, we investigated the developmental changes in the distribution of CS projection neurons in cynomolgus monkey (Macaca fascicularis). Injections of retrograde tracer at cervical levels of the spinal cord at embryonic day (E) 95 and E105 show that: (i) areal distribution of back-labeled neurons is more extensive than in the neonate and dense labeling is found in prefrontal, limbic, temporal, and occipital cortex; (ii) distributions of contralateral and ipsilateral projecting CS neurons are comparable in terms of location and numbers of labeled neurons, in contrast to the adult where the contralateral projection is an order of magnitude higher than the ipsilateral projection. Findings from one largely restricted injection suggest a hitherto unsuspected early innervation of the gray matter. In the fetus there was in addition dense labeling in the central nucleus of the amygdala, the hypothalamus, the subthalamic nucleus, and the adjacent region of the zona incerta, subcortical structures with only minor projections in the adult control.


Assuntos
Encéfalo/citologia , Encéfalo/embriologia , Neurônios/fisiologia , Tratos Piramidais/citologia , Tratos Piramidais/embriologia , Animais , Axônios/fisiologia , Macaca fascicularis , Vias Neurais/citologia , Vias Neurais/embriologia , Técnicas de Rastreamento Neuroanatômico
4.
Cereb Cortex ; 30(3): 1407-1421, 2020 03 14.
Artigo em Inglês | MEDLINE | ID: mdl-31504286

RESUMO

There is an extensive modification of the functional organization of the brain in the congenital blind human, although there is little understanding of the structural underpinnings of these changes. The visual system of macaque has been extensively characterized both anatomically and functionally. We have taken advantage of this to examine the influence of congenital blindness in a macaque model of developmental anophthalmia. Developmental anophthalmia in macaque effectively removes the normal influence of the thalamus on cortical development leading to an induced "hybrid cortex (HC)" combining features of primary visual and extrastriate cortex. Here we show that retrograde tracers injected in early visual areas, including HC, reveal a drastic reduction of cortical projections of the reduced lateral geniculate nucleus. In addition, there is an important expansion of projections from the pulvinar complex to the HC, compared to the controls. These findings show that the functional consequences of congenital blindness need to be considered in terms of both modifications of the interareal cortical network and the ascending visual pathways.


Assuntos
Cegueira/congênito , Corpos Geniculados/fisiopatologia , Córtex Visual/fisiopatologia , Vias Visuais/fisiologia , Animais , Cegueira/fisiopatologia , Mapeamento Encefálico/métodos , Feminino , Corpos Geniculados/fisiologia , Macaca fascicularis , Masculino , Neurônios/fisiologia , Tálamo/fisiologia , Tálamo/fisiopatologia , Córtex Visual/fisiologia , Vias Visuais/fisiopatologia
5.
Cereb Cortex ; 28(8): 3017-3034, 2018 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-29850900

RESUMO

There is little understanding of the structural underpinnings of the functional reorganization of the cortex in the congenitally blind human. Taking advantage of the extensive characterization of the macaque visual system, we examine in macaque the influence of congenital blindness resulting from the removal of the retina during in utero development. This effectively removes the normal influence of the thalamus on cortical development leading to an induced hybrid cortex (HC) combining features of primary visual and extrastriate cortex. Retrograde tracers injected in HC reveal a local, intrinsic connectivity characteristic of higher order areas and show that the HC receives a uniquely strong, purely feedforward projection from striate cortex but no ectopic inputs, except from subiculum, and entorhinal cortex. Statistical modeling of quantitative connectivity data shows that HC is relatively high in the cortical hierarchy and receives a reinforced input from ventral stream areas while the overall organization of the functional streams are conserved. The directed and weighted anophthalmic cortical graph from the present study can be used to construct dynamic and structural models. These findings show how the sensory periphery governs cortical phenotype and reveal the importance of developmental arealization for understanding the functional reorganization in congenital blindness.


Assuntos
Mapeamento Encefálico , Amaurose Congênita de Leber/patologia , Neurônios/fisiologia , Córtex Visual/patologia , Córtex Visual/fisiopatologia , Vias Visuais/fisiopatologia , Animais , Modelos Animais de Doenças , Macaca fascicularis , Rede Nervosa/patologia , Pentobarbital/metabolismo
6.
J Comp Neurol ; 522(1): 225-59, 2014 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-23983048

RESUMO

The laminar location of the cell bodies and terminals of interareal connections determines the hierarchical structural organization of the cortex and has been intensively studied. However, we still have only a rudimentary understanding of the connectional principles of feedforward (FF) and feedback (FB) pathways. Quantitative analysis of retrograde tracers was used to extend the notion that the laminar distribution of neurons interconnecting visual areas provides an index of hierarchical distance (percentage of supragranular labeled neurons [SLN]). We show that: 1) SLN values constrain models of cortical hierarchy, revealing previously unsuspected areal relations; 2) SLN reflects the operation of a combinatorial distance rule acting differentially on sets of connections between areas; 3) Supragranular layers contain highly segregated bottom-up and top-down streams, both of which exhibit point-to-point connectivity. This contrasts with the infragranular layers, which contain diffuse bottom-up and top-down streams; 4) Cell filling of the parent neurons of FF and FB pathways provides further evidence of compartmentalization; 5) FF pathways have higher weights, cross fewer hierarchical levels, and are less numerous than FB pathways. Taken together, the present results suggest that cortical hierarchies are built from supra- and infragranular counterstreams. This compartmentalized dual counterstream organization allows point-to-point connectivity in both bottom-up and top-down directions.


Assuntos
Neurônios/citologia , Córtex Visual/anatomia & histologia , Vias Visuais/anatomia & histologia , Animais , Retroalimentação Sensorial , Feminino , Macaca fascicularis , Macaca mulatta , Masculino , Técnicas de Rastreamento Neuroanatômico , Córtex Visual/citologia , Vias Visuais/citologia
7.
Proc Natl Acad Sci U S A ; 110(13): 5187-92, 2013 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-23479610

RESUMO

We investigated the influence of interareal distance on connectivity patterns in a database obtained from the injection of retrograde tracers in 29 areas distributed over six regions (occipital, temporal, parietal, frontal, prefrontal, and limbic). One-third of the 1,615 pathways projecting to the 29 target areas were reported only recently and deemed new-found projections (NFPs). NFPs are predominantly long-range, low-weight connections. A minimum dominating set analysis (a graph theoretic measure) shows that NFPs play a major role in globalizing input to small groups of areas. Randomization tests show that (i) NFPs make important contributions to the specificity of the connectivity profile of individual cortical areas, and (ii) NFPs share key properties with known connections at the same distance. We developed a similarity index, which shows that intraregion similarity is high, whereas the interregion similarity declines with distance. For area pairs, there is a steep decline with distance in the similarity and probability of being connected. Nevertheless, the present findings reveal an unexpected binary specificity despite the high density (66%) of the cortical graph. This specificity is made possible because connections are largely concentrated over short distances. These findings emphasize the importance of long-distance connections in the connectivity profile of an area. We demonstrate that long-distance connections are particularly prevalent for prefrontal areas, where they may play a prominent role in large-scale communication and information integration.


Assuntos
Mapeamento Encefálico , Córtex Cerebral , Bases de Dados Factuais , Rede Nervosa , Animais , Córtex Cerebral/anatomia & histologia , Córtex Cerebral/fisiologia , Macaca , Rede Nervosa/anatomia & histologia , Rede Nervosa/fisiologia
8.
Stem Cells ; 26(6): 1444-53, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-18356572

RESUMO

Embryonic stem cells (ESC) have the ability of indefinite self-renewal and multilineage differentiation, and they carry great potential in cell-based therapies. The rhesus macaque is the most relevant preclinical model for assessing the benefit, safety, and efficacy of ESC-based transplantations in the treatment of neurodegenerative diseases. In the case of neural cell grafting, tracing both the neurons and their axonal projections in vivo is essential for studying the integration of the grafted cells in the host brain. Tau-Green fluorescent protein (tau-GFP) is a powerful viable lineage tracer, allowing visualization of cell bodies, dendrites, and axons in exquisite detail. Here, we report the first rhesus monkey ESC line that ubiquitously and stably expresses tau-GFP. First, we derived a new line of rhesus monkey ESC (LYON-ES1) that show marker expression and cell cycle characteristics typical of primate ESCs. LYON-ES1 cells are pluripotent, giving rise to derivatives of the three germ layers in vitro and in vivo through teratoma formation. They retain all their undifferentiated characteristics and a normal karyotype after prolonged culture. Using lentiviral infection, we then generated a monkey ESC line stably expressing tau-GFP that retains all the characteristics of the parental wild-type line and is clonogenic. We show that neural precursors derived from the tau-GFP ESC line are multipotent and that their fate can be precisely mapped in vivo after grafting in the adult rat brain. Disclosure of potential conflicts of interest is found at the end of this article.


Assuntos
Células-Tronco Embrionárias/fisiologia , Proteínas de Fluorescência Verde/genética , Proteínas tau/genética , Fosfatase Alcalina/metabolismo , Animais , Blastocisto/citologia , Diferenciação Celular , Linhagem Celular , Células-Tronco Embrionárias/citologia , Células-Tronco Embrionárias/enzimologia , Células-Tronco Embrionárias/virologia , Genes Reporter , Lentivirus , Macaca mulatta , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transplante de Células-Tronco/métodos , Teratoma/genética , Teratoma/patologia , Transfecção , Zona Pelúcida/fisiologia
9.
Cereb Cortex ; 16 Suppl 1: i26-34, 2006 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16766704

RESUMO

Regionalization of cell cycle kinetics of cortical precursors has been described in nonhuman primates and rodents indicating a fate map of areas as distinct proliferative programs in the germinal zones of the neocortex. It remains to be understood how proliferative gradients during corticogenesis are transcribed into a stepwise function to form adult areal borders. Here we have used the monkey areas 17 and 18, which show striking cytoarchitectonic differences, as a model system for studying how developmental events establish areal boundaries in the adult. We present data indicating that the events that are involved in the formation of a sharp border separating 2 areas involve an orchestration of diverse phenomena including differential rates of proliferation, migration, and tangential expansion.


Assuntos
Neocórtex/citologia , Neocórtex/embriologia , Rede Nervosa/citologia , Rede Nervosa/embriologia , Neurônios/citologia , Neurônios/fisiologia , Organogênese/fisiologia , Animais , Padronização Corporal/fisiologia , Diferenciação Celular/fisiologia , Proliferação de Células , Haplorrinos , Neocórtex/fisiologia , Rede Nervosa/fisiologia
10.
Neuron ; 47(3): 353-64, 2005 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-16055060

RESUMO

We have investigated the cell cycle-related mechanisms that lead to the emergence of primate areas 17 and 18. These areas are characterized by striking differences in cytoarchitectonics and neuron number. We show in vivo that (1) area 17 precursors of supragranular neurons exhibit a shorter cell cycle duration, a reduced G1 phase, and a higher rate of cell cycle reentry than area 18 precursors; (2) area 17 and area 18 precursors show contrasting and specific levels of expression of cyclin E (high in area 17, low in area 18) and p27Kip1 (low in area 17, high in area 18); (3) ex vivo up- and downmodulation of cyclin E and p27Kip1 show that both regulators influence cell cycle kinetics by modifying rates of cell cycle progression and cell cycle reentry; (4) modeling the areal differences in cell cycle parameters suggests that they contribute to areal differences in numbers of precursors and neuron production.


Assuntos
Ciclo Celular/fisiologia , Córtex Cerebral/citologia , Córtex Cerebral/embriologia , Fase G1/fisiologia , Macaca fascicularis/embriologia , Animais , Proteínas de Ciclo Celular/metabolismo , Ciclina E/metabolismo , Inibidor de Quinase Dependente de Ciclina p27 , Regulação para Baixo , Desenvolvimento Fetal , Feto/metabolismo , Técnicas In Vitro , Cinética , Modelos Neurológicos , Neurônios/citologia , Fatores de Tempo , Proteínas Supressoras de Tumor/metabolismo , Regulação para Cima
11.
Cereb Cortex ; 12(5): 453-65, 2002 May.
Artigo em Inglês | MEDLINE | ID: mdl-11950763

RESUMO

The laminar organization of cortico-cortical projection neurons (expressed by the percentage of supragranular projecting neurons - SLN%) characterizes cortical pathways as feedforward (FF) or feedback (FB) and determines the hierarchical ranking of cortical areas. There is evidence of a developmental reduction in SLN% of pathways to area V1. Here, by analyzing pre- and postnatal projections to area V4, we have been able to address whether developmental reductions of SLN% impact on information processing in the immature cortex. FB pathways to area V4 exhibit 28-84% reduction of SLN%. This contrasts with the FF projections, which show little or no SLN% reduction. However, SLN% values in the immature cortex allocated cortical areas to the same hierarchical levels as in the adult. The developmental reduction of SLN% is a widespread phenomenon in the neocortex and is a distinctive feature of FB pathways. Two mechanisms contribute to developmental changes in SLN%: (i) delayed ingrowth of axons into the cortical target from infragranular layer neurons and (ii) prolonged developmental reduction of the divergence of projections from supragranular layer neurons. The present results show that FF and FB projections exhibit different developmental processes and patterns of connections linking cortical areas and their hierarchical relations are established prenatally, independently of regressive phenomena.


Assuntos
Neurônios/fisiologia , Córtex Visual/embriologia , Córtex Visual/crescimento & desenvolvimento , Animais , Animais Recém-Nascidos/anatomia & histologia , Feminino , Macaca fascicularis , Vias Neurais/anatomia & histologia , Vias Neurais/embriologia , Vias Neurais/crescimento & desenvolvimento , Gravidez , Córtex Visual/anatomia & histologia
12.
Cereb Cortex ; 12(1): 37-53, 2002 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-11734531

RESUMO

We examined the development of the occipital lobe in fetal monkeys between embryonic day 37 (E37) and E108 in Nissl-stained and acetylcholine esterase (AChE)-reacted sections. We paid particular attention to features that distinguish the development of presumptive area 17. At E46 the neuroepithelium consists of a ventricular zone and a monolayer cortical plate sandwiched between a thin marginal zone and a minimal presubplate. Between E55 and E65 an augmented subplate emerges and continues to expand up to E94 to become a major compartment of the developing cortex. A mitotic subventricular zone is established by E55. Peaking in depth at E72, it constitutes the principal germinal zone. By E78 an invading fibre tract divides it into an outer radially organized zone and a more conventional inner zone. AChE staining reveals the future area 17/18 border from E86 onwards. Proceeding from presumptive area 17 to area 18 there is a progressive thinning of the radially structured subventricular zone. Comparison of these results with corticogenesis in rodents suggests a number of potentially unique primate features: (i) a minimal preplate stage; (ii) a radially augmented germinal zone not previously described in non-primates; (iii) a fibre tract dividing the subventricular zone into two laminae; (iv) late generation and expansion of the subplate.


Assuntos
Neurônios/fisiologia , Lobo Occipital/anatomia & histologia , Lobo Occipital/embriologia , Córtex Visual/anatomia & histologia , Córtex Visual/embriologia , Acetilcolinesterase/metabolismo , Animais , Células Epiteliais/enzimologia , Células Epiteliais/fisiologia , Epitélio/embriologia , Epitélio/enzimologia , Feminino , Imuno-Histoquímica , Macaca fascicularis , Camundongos , Mitose/fisiologia , Neuropeptídeo Y/metabolismo , Lobo Occipital/enzimologia , Gravidez , Telencéfalo/citologia , Telencéfalo/embriologia , Córtex Visual/enzimologia
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