RESUMO
Mycobacterium arupense is a novel mycobacterium species. It was first identified from clinical specimens in 2006 and since then there have been only two reports of its recovery from clinical samples. In the present case M. arupense was isolated from the sputum of a 62-year-old man with a malignant mass in his left kidney, who presented with a one-month history of recurrent fever, dyspnea and haemoptysis. M. arupense was identified with sequencing of hsp65 and 16S rRNA genes. In the present study, its biochemical profile along with its resistance status and hsp65 RFLP analysis is presented.
Assuntos
Técnicas de Tipagem Bacteriana , Impressões Digitais de DNA , Farmacorresistência Bacteriana , Infecções por Mycobacterium/diagnóstico , Mycobacterium/classificação , Mycobacterium/efeitos dos fármacos , Polimorfismo de Fragmento de Restrição , Proteínas de Bactérias/genética , Chaperonina 60/genética , DNA Bacteriano/genética , DNA Ribossômico/genética , Humanos , Neoplasias Renais/complicações , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Mycobacterium/genética , Mycobacterium/isolamento & purificação , Infecções por Mycobacterium/microbiologia , Infecções por Mycobacterium/patologia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Escarro/microbiologiaRESUMO
Mycobacterium thermoresistibile is a non-tuberculous mycobacterium strongly associated with human infections. Since 1966, there have only been six reports of its isolation from clinical samples. We report on the first case from Europe and review all the previous cases. Identification was achieved with sequencing of the 16S rRNA and hsp65 genes. This study presents its phenotypic and biochemical profile, susceptibilities to selected antibiotics and hsp65 polymerase chain reaction-restriction fragment length polymorphism profile with BsteII and Hae III .
Assuntos
Infecções por Mycobacterium/diagnóstico , Mycobacterium/classificação , Mycobacterium/isolamento & purificação , Idoso , Proteínas de Bactérias/genética , Técnicas de Tipagem Bacteriana , Chaperonina 60 , Chaperoninas/genética , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Europa (Continente) , Humanos , Masculino , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Infecções por Mycobacterium/microbiologia , Filogenia , Polimorfismo de Fragmento de Restrição , RNA Ribossômico 16S/genética , Análise de Sequência de DNARESUMO
Mycobacterium malmoense was isolated from a broncho-alveolar lavage sample of a 73-year-old cancer (small cell lung carcinoma) patient in Crete, representing the first reported case of this pathogen in Greece. The isolate was considered to be a colonizer and the patient did not receive any antimycobacterial treatment while he received chemotherapy to which he responded favourably. No signs of pulmonary infection were noted during the course of his disease. This case provides evidence of the ubiquitous nature of this mycobacterial species, believed until recently to favour cooler climates. We, therefore, propose that the index of suspicion for this pathogen should be raised particularly in patients with underlying immunodeficiency, cancer and chronic lung disease, irrespective of the geographic location.
Assuntos
Líquido da Lavagem Broncoalveolar/microbiologia , Mycobacterium/isolamento & purificação , Idoso , Carcinoma de Células Pequenas , Grécia , Humanos , Neoplasias Pulmonares , MasculinoAssuntos
Mycobacterium tuberculosis/efeitos dos fármacos , Tuberculose Resistente a Múltiplos Medicamentos/epidemiologia , Emigração e Imigração , Grécia/epidemiologia , Humanos , Testes de Sensibilidade Microbiana , Mycobacterium tuberculosis/genética , Tuberculose Resistente a Múltiplos Medicamentos/tratamento farmacológicoAssuntos
Técnicas de Tipagem Bacteriana , DNA Bacteriano/análise , Mycobacterium tuberculosis/classificação , Mycobacterium/classificação , Kit de Reagentes para Diagnóstico , Animais , Bovinos , Genótipo , Humanos , Mycobacterium/genética , Mycobacterium/isolamento & purificação , Infecções por Mycobacterium/microbiologia , Mycobacterium bovis/classificação , Mycobacterium bovis/genética , Mycobacterium bovis/isolamento & purificação , Mycobacterium tuberculosis/genética , Mycobacterium tuberculosis/isolamento & purificação , Especificidade da EspécieRESUMO
A combination of Bactec MGIT 960 system, a PCR-based assay and a PCR-Restriction Analysis procedure (PRA) was assessed for the detection and identification of mycobacteria from clinical samples. The MGIT recovered 243 from 266 mycobacterial isolates. The PCR-based assay correctly identified all (234) Mycobacterium tuberculosis isolates, while 35 nontuberculous mycobacterial isolates were identified by PRA.