RESUMO
Some immunity parameters (interferon, tumor necrosis factor, interleukin 1, etc.) were studied in CBA/Calac mice infected with Lassa virus. The results permit a hypothesis that a pathologic inflammatory reaction is responsible for the death of animals in experimental Lassa fever. One of the components of this reaction is endogenous shock involving a manifest production of immune response mediators, such as interferon, interleukin 1. and tumor necrosis factor.
Assuntos
Febre Lassa/imunologia , Animais , Linhagem Celular , Chlorocebus aethiops , Citotoxicidade Imunológica , Humanos , Interferons/biossíntese , Interleucina-1/biossíntese , Células Matadoras Naturais/imunologia , Febre Lassa/metabolismo , Ativação Linfocitária , Camundongos , Camundongos Endogâmicos CBA , Células Tumorais Cultivadas , Fator de Necrose Tumoral alfa/biossíntese , Células VeroRESUMO
The parameters of humoral and cell-mediated immunity after inoculation of the infectious and inactivated antigen of Lassa virus to rabbits which are insusceptible to this infection were studied. Lassa virus antigen was demonstrated to induce synthesis of specific antibodies in rabbits the level of which was proportional to the dose of inoculation and the time of immunization. The results of leukocyte blastogenesis test and delayed type hypersensitivity test confirm the development of sensitization to inactivated Lassa virus in immune rabbits.
Assuntos
Arenaviridae/imunologia , Imunização , Vírus Lassa/imunologia , Animais , Formação de Anticorpos , Imunidade Celular , CoelhosRESUMO
In experimental infection of mice with Lassa virus, the infectious virus could be detected in all the organs and brain tissues tested. Histopathological lesions were demonstrated in cerebral and spinal cord tissues only. Roentgen irradiation in a dose of 500 R and cyclophosphamide protected mice against a lethal Lassa virus dose. Cyclosporin A in various doses exerted no effect on the outcome of the acute infection. The adoptive transfer of splenocytes from mouse donors inoculated intraperitoneally prevented the development of lethal disease symptoms and death of mice-recipients. It is suggested that immunocompetent cells are involved in the development and outcome of experimental infection of mice with Lassa virus.
Assuntos
Tolerância Imunológica , Febre Lassa/etiologia , Doença Aguda , Animais , Ciclofosfamida/farmacologia , Ciclosporinas/farmacologia , Tolerância Imunológica/efeitos dos fármacos , Tolerância Imunológica/efeitos da radiação , Imunidade Celular/efeitos dos fármacos , Imunidade Celular/efeitos da radiação , Imunização Passiva , Febre Lassa/imunologia , Febre Lassa/microbiologia , Vírus Lassa/efeitos dos fármacos , Vírus Lassa/isolamento & purificação , Vírus Lassa/fisiologia , Vírus Lassa/efeitos da radiação , Camundongos , Camundongos Endogâmicos C3H , Camundongos Endogâmicos CBA , Replicação Viral/efeitos dos fármacos , Replicação Viral/efeitos da radiaçãoRESUMO
The influence of humoral and cell-mediated immunity on the formation of resistance in mice immunized with inactivated Lassa virus was evaluated. As a result of immunization, both nonspecific and specific immune response developed, the main rule in the formation of resistance belonging to specific immunity.