Buoyancy driven mixing of miscible fluids by volumetric energy deposition of microwaves.

An experiment that seeks to investigate buoyancy driven mixing of miscible fluids by microwave volumetric energy deposition is presented. The experiment involves the use of a light, non-polar fluid that initially rests on top of a heavier fluid which is more polar. Microwaves preferentially heat the polar fluid, and its density decreases due to thermal expansion. As the microwave heating continues, the density of the lower fluid eventually becomes less than that of the upper, and buoyancy driven Rayleigh-Taylor mixing ensues. The choice of fluids is crucial to the success of the experiment, and a description is given of numerous fluid combinations considered and characterized. After careful consideration, the miscible pair of toluene/tetrahydrofuran (THF) was determined as having the best potential for successful volumetric energy deposition buoyancy driven mixing. Various single fluid calibration experiments were performed to facilitate the development of a heating theory. Thereafter, results from two-fluid mixing experiments are presented that demonstrate the capability of this novel Rayleigh-Taylor driven experiment. Particular interest is paid to the onset of buoyancy driven mixing and unusual aspects of the experiment in the context of typical Rayleigh-Taylor driven mixing.

Biomimetic monolayer and bilayer membranes made from amphiphilic block copolymer micelles.

The deposition of amphiphilic poly(ethylene oxide)-block-poly(butadiene) (PEO-b-PBD) copolymer micelles is demonstrated on solid substrates. Depending upon surface chemistry, micelle adsorption creates either monolayer or bilayer films. Lateral diffusion measurements reveal that strong coupling between hydrophilic surfaces and PEO blocks creates immobile bilayers, while monolayers retain the fluidity previously observed in vesicular assemblies.

Lipid bilayer reorganization under extreme pH conditions.

Supported lipid bilayers containing phosphatidylcholine headgroups are observed to undergo reorganization from a 2D fluid, lipid bilayer assembly into an array of complex 3D structures upon exposure to extreme pH environments. These conditions induce a combination of molecular packing and electrostatic interactions that can create dynamic morphologies of highly curved lipid membrane structures. This work demonstrates that fluid, single-component lipid bilayer assemblies can create complex morphologies, a phenomenon typically only associated with lipid bilayers of mixed composition.

Substrate effects on interactions of lipid bilayer assemblies with bound nanoparticles.

Understanding the interactions of nanoparticles with lipid membranes is crucial in establishing the mechanisms that govern assembly of membrane-based nanocomposites, nanotoxicology, and biomimetic inspired self-assembly. In this study, we explore binding of charged nanoparticles to lipid bilayers, both as liposomes and substrate supported assemblies. We find that the presence of a solid-support, regardless of curvature, eliminates the ability of zwitterionic fluid phase lipids to bind charged nanoparticles.

Hydrophilicity and the viscosity of interfacial water.

We measure the viscosity of nanometer-thick water films at the interface with an amorphous silica surface. We obtain viscosity values from three different measurements: friction force in a water meniscus formed between an oxide-terminated W tip and the silica surface under ambient conditions; similar measurements for these interfaces under water; and the repulsive "drainage" force as the two surfaces approach at various speeds in water. In all three cases, we obtain effective viscosities that are approximately 10(6) times greater than that of bulk water for nanometer-scale interfacial separations. This enhanced viscosity is not observed when we degrade the hydrophilicity of the surface by terminating it with -H or -CH3. In view of recent results from other interfaces, we conclude that the criterion for the formation of a viscous interphase is the degree of hydrophilicity of the interfacial pair.