RESUMO
Ferric and ferrous were used to prepare the magnetic nanoparticles (MNPs), and concanavalin A was bound onto the MNPs as a magnetic nano-adsorbent for lactoferrin (Lf) separation. Scanning electron microscope showed that the diameter of modified MNPs was about 15.27⯱â¯1.42â¯nm. The results showed that the optimum adsorption and elution conditions of modified MNPs on recovery Lf were 4â¯min and 5â¯min, respectively. The specificity of modified MNPs on recovery Lf was high, and the purity of Lf in eluent was 93.06%. The recovery rate of modified MNPs from whey and elution were more than 99.99%, and the recovery rate of Lf from whey performed a dose-dependent relationship. The maximum adsorption capacity of modified MNPs on recovery Lf was 90⯵g/mg. The adsorption capacity of modified MNPs stored in phosphate buffer at 4⯰C significantly higher (Pâ¯<â¯0.05) than those stored in other experimental conditions.