[HPLC fingerprint of the antiarrhythmic fraction of Valeriana officinalis].

OBJECTIVE: To establish HPLC fingerprints of the Antiarrhythmic fraction of Valeriana officinalis. METHODS: Agilent C18 (250 mm x 4.6 mm, 5 microm) column was used and the acetonitrile-water was chosen as the mobile phase in a gradient mode. The column temperature was 380 degrees C and the detection wavelength was 218 nm. The detection time was 70 min, and the flow rate was 1.0 mL/ min. RESULTS: Fifteen characteristic peaks were indicated in HPLC fingerprints. The relative retention time and the ranges of relative areas of the common peaks were also determined. CONCLUSION: This method is simple and accurate with a good reproducibility and provides a reference standard for the quality control of Valeriana officinalis.