Application of Biotechniques for In Vitro Virus and Viroid Elimination in Pome Fruit Crops.

Sustainable production of pome fruit crops is dependent upon having virus-free planting materials. The production and distribution of plants derived from virus- and viroid-negative sources is necessary not only to control pome fruit viral diseases but also for sustainable breeding activities, as well as the safe movement of plant materials across borders. With variable success rates, different in vitro-based techniques, including shoot tip culture, micrografting, thermotherapy, chemotherapy, and shoot tip cryotherapy, have been employed to eliminate viruses from pome fruits. Higher pathogen eradication efficiencies have been achieved by combining two or more of these techniques. An accurate diagnosis that confirms complete viral elimination is crucial for developing effective management strategies. In recent years, considerable efforts have resulted in new reliable and efficient virus detection methods. This comprehensive review documents the development and recent advances in biotechnological methods that produce healthy pome fruit plants. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Impact of COVID-19 in pediatric oncology care in Latin America during the first year of the pandemic.

BACKGROUND: The ongoing coronavirus 2019 disease (COVID-19) pandemic strained medical systems worldwide. We report on the impact on pediatric oncology care in Latin American (LATAM) during its first year. METHOD: Four cross-sectional surveys were electronically distributed among pediatric onco-hematologists in April/June/October 2020, and April/2021 through the Latin American Society of Pediatric Oncology (SLAOP) email list and St Jude Global regional partners. RESULTS: Four hundred fifty-three pediatric onco-hematologists from 20 countries responded to the first survey, with subsequent surveys response rates above 85%. More than 95% of participants reported that treatment continued without interruption for new and active ongoing patients, though with disruptions in treatment availability. During the first three surveys, respondents reported suspensions of outpatient procedures (54.2%), a decrease in oncologic surgeries (43.6%), radiotherapy (28.4%), stem cell transplants (SCT) (69.3%), and surveillance consultations (81.2%). Logistic regression analysis showed that at the beginning of the first wave, participants from countries with healthcare expenditure below 7% were more likely to report a decrease in outpatient procedures (odds ratio [OR]: 1.84, 95% CI: 1.19-2.8), surgeries (OR: 3, 95% CI: 1.9-4.6) and radiotherapy (OR: 6, 95% CI: 3.5-10.4). Suspension of surveillance consultations was higher in countries with COVID-19 case fatality rates above 2% (OR: 3, 95% CI: 1.4-6.2) and SCT suspensions in countries with COVID-19 incidence rate above 100 cases per 100,000 (OR: 3.48, 95% CI: 1.6-7.45). Paradoxically, at the beginning of the second wave with COVID-19 cases rising exponentially, most participants reported improvements in cancer services availability. CONCLUSION: Our data show the medium-term collateral effects of the pandemic on pediatric oncology care in LATAM, which might help delineate oncology care delivery amid current and future challenges posed by the pandemic.

Different loci control resistance to different isolates of the same race of Colletotrichum lindemuthianum in common bean.

KEY MESSAGE: Linkage and genome-wide association analyses using high-throughput SNP genotyping revealed different loci controlling resistance to different isolates of race 65 of Colletotrichum lindemuthianum in common bean. Development of varieties with durable resistance to anthracnose is a major challenge in common bean breeding programs because of the extensive virulence diversity of Colletotrichum lindemuthianum fungus. We used linkage and genome-wide association analyses to tap the genomic regions associated with resistance to different isolates of race 65. Linkage mapping was done using an F2 population derived from the cross between the Mesoamerican common beans BRS Estilo x Ouro Vermelho, inoculated with two different isolates of race 65. Association genetics relied on a diversity common bean panel containing 189 common bean accessions inoculated with five different isolates of race 65 as an attempt to validate the linkage analysis findings and, eventually, identify other genomic regions associated with resistance to race 65. The F2 population and diversity panel were genotyped with the BARCBean6K_3 Illumina BeadChip containing 5398 SNP markers. Both linkage and genome-wide association analyses identified different loci controlling resistance to different isolates of race 65 on linkage group Pv04. Genome-wide association analysis also detected loci on Pv05, Pv10 and Pv11 associated with resistance to race 65. These findings indicate that resistance to race 65 can be overcome by the virulence diversity among different isolates of the same race and could lead to the loss of resistance after cultivar release. We identified 25 resistant common bean cultivars to all five isolates of race 65 in the diversity panel. The accessions should be useful to develop cultivars combining different resistance genes that favor durable resistance to anthracnose in common bean.

Fine mapping of an anthracnose-resistance locus in Andean common bean cultivar Amendoim Cavalo.

Anthracnose, caused by the fungal pathogen Colletotrichum lindemuthianum, is one of the world's most destructive diseases of common bean. The use of resistant cultivars is the most cost-effective strategy to manage this disease; however, durable resistance is difficult to achieve due to the vast virulence diversity of the anthracnose pathogen. Finding new genes with broad-spectrum resistance increases the prospect of designing an effective anthracnose-management strategy. Genetic analysis confirmed the presence of a single, dominant anthracnose-resistance locus in AC, which we provisionally named Co-AC. Bulk segregant analysis and genetic mapping of two F2 populations from the crosses AC × PI207262 and AC × G 2333 were used to determine the position of the Co-AC locus in a 631 Kbp genomic region flanked by the SNP markers SS56 and SS92 on the lower arm of chromosome Pv01. By genotyping 77 F3 plants from the AC × PI207262 cross using nine additional markers, we fine-mapped the Co-AC locus to a significantly smaller genomic region (9.4 Kbp) flanked by the SNP markers SS102 and SS165. This 9.4 Kbp region harbors three predicted genes based on the common bean reference genome, notably including the gene model Phvul.001G244300, which encodes Clathrin heavy chain 1, a protein that supports specific stomatal regulation functions and might play a role in plant defense signaling. Because the Co-AC resistance locus is linked in cis, it can be selected with great efficiency using molecular markers. These results will be very useful for breeding programs aimed at developing bean cultivars with anthracnose resistance using marker-assisted selection. This study revealed the broad-spectrum resistance of AC to C. lindemuthianum and the existence of the Co-AC anthracnose-resistance locus. Fine mapping positioned this locus in a small genomic region on the lower end of chromosome Pv01 that contained three candidate genes for the Co-AC locus.

High-resolution mapping reveals linkage between genes in common bean cultivar Ouro Negro conferring resistance to the rust, anthracnose, and angular leaf spot diseases.

KEY MESSAGE: Co-segregation analysis and high-throughput genotyping using SNP, SSR, and KASP markers demonstrated genetic linkage between Ur-14 and Co-3 4 /Phg-3 loci conferring resistance to the rust, anthracnose and angular leaf spot diseases of common bean. Rust, anthracnose, and angular leaf spot are major diseases of common bean in the Americas and Africa. The cultivar Ouro Negro has the Ur-14 gene that confers broad spectrum resistance to rust and the gene cluster Co-3 4 /Phg-3 containing two tightly linked genes conferring resistance to anthracnose and angular leaf spot, respectively. We used co-segregation analysis and high-throughput genotyping of 179 F2:3 families from the Rudá (susceptible) × Ouro Negro (resistant) cross-phenotyped separately with races of the rust and anthracnose pathogens. The results confirmed that Ur-14 and Co-3 4 /Phg-3 cluster in Ouro Negro conferred resistance to rust and anthracnose, respectively, and that Ur-14 and the Co-3 4 /Phg-3 cluster were closely linked. Genotyping the F2:3 families, first with 5398 SNPs on the Illumina BeadChip BARCBEAN6K_3 and with 15 SSR, and eight KASP markers, specifically designed for the candidate region containing Ur-14 and Co-3 4 /Phg-3, permitted the creation of a high-resolution genetic linkage map which revealed that Ur-14 was positioned at 2.2 cM from Co-3 4 /Phg-3 on the short arm of chromosome Pv04 of the common bean genome. Five flanking SSR markers were tightly linked at 0.1 and 0.2 cM from Ur-14, and two flanking KASP markers were tightly linked at 0.1 and 0.3 cM from Co-3 4 /Phg-3. Many other SSR, SNP, and KASP markers were also linked to these genes. These markers will be useful for the development of common bean cultivars combining the important Ur-14 and Co-3 4 /Phg-3 genes conferring resistance to three of the most destructive diseases of common bean.

Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma.

BACKGROUND: The Andean cultivar Paloma is resistant to Mesoamerican and Andean races of Colletotrichum lindemuthianum, the fungal pathogen that causes the destructive anthracnose disease in common bean. Remarkably, Paloma is resistant to Mesoamerican races 2047 and 3481, which are among the most virulent races of the anthracnose pathogen. Most genes conferring anthracnose resistance in common bean are overcome by these races. The genetic mapping and the relationship between the resistant Co-Pa gene of Paloma and previously characterized anthracnose resistance genes can be a great contribution for breeding programs. RESULTS: The inheritance of resistance studies for Paloma was performed in F2 population from the cross Paloma (resistant) × Cornell 49-242 (susceptible) inoculated with race 2047, and in F2 and F2:3 generations from the cross Paloma (resistant) × PI 207262 (susceptible) inoculated with race 3481. The results of these studies demonstrated that a single dominant gene confers the resistance in Paloma. Allelism tests performed with multiple races of C. lindemuthianum showed that the resistance gene in Paloma, provisionally named Co-Pa, is independent from the anthracnose resistance genes Co-1, Co-2, Co-3, Co-4, Co-5, Co-6, Co-12, Co-13, Co-14, Co-15 and Co-16. Bulk segregant analysis using the SNP chip BARCBean6K_3 positioned the approximate location of Co-Pa in the lower arm of chromosome Pv01. Further mapping analysis located the Co-Pa gene at a 390 kb region of Pv01 flanked by SNP markers SS82 and SS83 at a distance of 1.3 and 2.1 cM, respectively. CONCLUSIONS: The results presented here showed that Paloma cultivar has a new dominant gene conferring resistance to anthracnose, which is independent from those genes previously described. The linkage between the Co-Pa gene and the SS82 and SS83 SNP markers will be extremely important for marker-assisted introgression of the gene into elite cultivars in order to enhance resistance.

Relación de caries dental y fluorosis en escolares de cuatro localidades termales del departamento de Potosí-Bolivia / Relationship between dental caries and fluorosis in schoolboys and schoolgirls of four thermal localities from Potosí-Bolivia

El presente trabajo es una recopilación de diferentes investigaciones realizadas por la misma autora, desde al año 2005 al 2008 en localidades termales ubicadas en las cercanías de la ciudad de Potosí - Bolivia, (Tarapaya, Miraflores, Chaqui y Don Diego). Los objetivos fueron evaluar la presencia de fluorosis dental, caries dental, hábitos higiénicos-alimenticios en escolares de estas regiones. Se determinó caries dental con el índice CPO-D, para Tarapaya 0.75, Miraflores 1.4, Chaqui 0.8, Don Diego 2.29. En el índice ceo-d se ubicó 4.7 Tarapaya, Miraflores 5.7, Chaqui 4.4 y Don Diego 3.69. Se estableció fluorosis dental con el índice de Dean. En Chaqui se encontró en dientes permanentes hasta el grado 4 (8,1%) y en piezas temporales no se evidenció. No se encontró fluorosis en Tarapaya ni Miraflores. En relación al consumo de alimentos con contenido de calcio, hidratos de carbono y azúcar refinada, la mayoría, sí consumen. Con referencia al consumo de aguas termales, no se realiza en Tarapaya, es escaso en Miraflores, en Chaqui y Don Diego el consumo es mixto. Se recomienda la implementación de un Programa de atención primaria en salud para caries y fluorosis dental. Asimismo ampliar el estudio de análisis químico de flúor en aguas en todas sus fuentes.

The present work is a compilation of different research projects made by the same author, from 2005 to 2008 in thermal localities placed near Potosí - Bolivia (Tarapaya, Miraflores, Chaquí and Don Diego). The objectives were to evaluate the presence of dental fluorosis, dental cavities, hygiene - nourishing habits in schoolboys and schoolgirls from these regions. The dental caries indexesusing DMFT was for Tarapaya 0.75, Miraflores 1.4. Chaquí 0.8, Don Diego 2.29. in the index dmft was 4.7 Tarapaya, Miraflores 5.7, Chaquí 4.4 and Don Diego 3.69. Dental fluorosis was established with the Dean index. In Chaquí it was found grade 5 (6,5%) in permanent teeth and grade 2 (1,6%), in temporal teeth till and it was not demonstrated in primary teeth. Fluorosis was found neither in Tarapaya nor in Miraflores. There was a relation between nourishing and the content of calcium, carbohydrates and refined sugar. Consumption of thermal water was limited to Miraflores and it was nor consumed in Tarapaya, in Chaquí and Don Diego the consumption was mixed. Its is recommended the implementation of a program for primary health attention for dental caries and dental fluorosis. This study should be completed with chemical analysis of fluoride in the water.

Ortoptest una nueva visión para el futuro / Ortoptest a new vision for the future

Este fue un proyecto realizado por cinco estudiantes de octavo semestre de la Facultad de Optometría de la Universidad de la Salle. Ortoptest es un aparato en forma de cubo que en cada una de sus caras dispone de test para un mejor diagnóstico. Estos son: Ishihara´s (14 laminas acrílicas), luces de Worth, rejillas de Amsler y test bicromado rojo-verde (para visión próxima). Dentro del cubo se encuentra un sistema de iluminación halógena de 25w, tiene una base giratoria que hace más fácil y rápido el desarrollo de los test agilizando el tiempo de consulta. Se realizó un estudio de tipo descriptivo, cuyo objetivo fue determinar la aceptación del Ortoptest entre los profesionales de la salud visual y Ocular (análisis de mercado), se empleó una muestra de 15 estudiantes de la Facultad de Optometría ULS de diferentes semestres, 10 Optómetras y 10 Oftalmólogos. A los integrantes de la muestra se les aplicó una encuesta con cinco preguntas cerradas y con una pregunta abierta para caracterizar el mercado objetivo. Las Principales respuestas estaban orientadas hacia la eficacia y la eficiencia del test en consulta, así como a las posibilidades de modificación, además el 74,27 por ciento de los encuestados lo obtendría por la disminución de tiempo en su consulta, así como por la integridad de los test. Los Optómetras pueden desarrollar ideas de negocios, innovadoras, solo es saber hallar las necesidades para poder darles solución, y, así demostrar que la Optometría es una ciencia que avanza en los negocios como en la tecnología.

[Hematopoietic stem cell transplantation: is it an immunologic therapy?]. / Trasplante de progenitores hematopoyéticos, es una terapia inmunológica?

The graft-versus-tumor effect occurring after allogeneic (genetically different) haematopoietic cell transplantation for treating human malignancies, represents the clearest example of the power of the human immune system to eradicate cancer. Recent advances in our understanding of the immunobiology of stem-cell engraftment, tolerance and tumor eradication are allowing clinicians to better harness this powerful effect.

Hematuria macroscópica persistente causada por el "Síndrome de cascanueces" / Persistent macroscopic hematuria caused by the nut cracker syndrome

We report the case a fifty nine years old male patient, with the nutcraker syndrome, with clinic characteristic of persisting macroscopic hematuria, radiologic and surgical evidence of compression of the left renal vein and consequent hypertension, by compression of the left kidney produced by an inflammatory pseudotumor of spleen.

Informe investigación caso control, brote de colera / Report Investigation case control, outbreak of colera

Como respuesta a la presentación de un brote de cólera en el Distrito IV de yacuiba un equipo de SNS,CCH,OPS,CDC viajaron a Yacuiba el 20 de enero para apoyar el control de la epidemia y realizar recomendaciones para la prevención de casos en el futuro

Sistemas de agua segura para consumo humano / Sake water for human use

Una gran parte de la población de nuestro país no tiene acceso al agua potable para el uso diario en sus hogares. En su lugar emplean agua de pozos, ríos, vertientes, arroyos y agua de cisternas de dudoso origen. Estas fuentes de agua tienen alta posibilidad de contaminación fecal, misma que representa un potencial riesgo para la salud. Por otro lado, los usuarios acostumbran a almacenar el agua para consumo diario en sus hogares, en recipientes de bocaancha, como baldes, latas, turriles, etc. que con frecuencia se recontaminan al introducir otros recipientes para sacar el agua. El principal objetivo de este artículo es enseñar a proteger la salud de la población reduciendo al mínimo o eliminando la contaminación del agua que en la mayoría de los casos es el vehículode las infecciones y epidemias. Al evaluar la calidad del agua, el consumidor depende por completo de sus sentidos, los componentes del agua pueden afectar su apariencia, olor y sabor y el consumidor evalua la calidad y aceptabilidad del agua, basándose esencialmente en esos criterios. Se considera así, peligrosa y se rechazará el agua que sea muy turbía, que tenga un color acentuado y un sabor desagradable. No obstante, ya no podemos confiar por completo en nuestros sentidoscuando se trata de juzgar la calidad microbiológica del agua. En su lugar debemos conseguir que la población tenga conocimiento sobre las posibilidades para contar con agua segura en el caso de no contar con redes de distribución de agua potable. Tres son los elementos básicos en este objetivo: Control de la contaminación fecal de las fuentes de agua

ESTUDIO MICROBIOLOGICO EN PACIENTES NIÑOS DE 3 A 12 AÑOS

Introducción, Estudio de la microbiología bucal en pacientes niños conprendidos entre a doce años,Generalidades saliva, Función secretora, Estimulo de las glandulas salivales, Humor bucal, Constitución y función bioquimica, Opalesencia, Indice del flujo salival, pH salival, Anticuerpos, Anticuerpos salivales, Corpusculos salivales o leucocitos, Identidad de los microorganismos responsables de destrucció dental, Factores biologicos en la saliva de posible importancia en la destrucción dental, Causas que frenan o destruyen la proliferación microbiana, La caries del niño en crecimiento, Odontología profilactica, Tartretomía y pulido, Pastas dentarias, Enjuagatorios y buchadas, Uso de la seda dental y mondadientes, Odontotomía, Material y metodos, Preparación, Observación directa, Cultivo en placa