Harnessing market based mechanisms to improve water quality: Water quality trading policies in the river Alde, UK.

Nitrogen fertiliser in agriculture continues to be one of the largest contributors to water pollution driven by the global food demand. Consequently, policies designed to tackle nitrogen pollution tend to be focused on the farm level. Applying mitigation measures requires knowledge, local labour and financial investment to achieve desired goals. Influencing farming activity comes with challenges as policies result in economic losses. We propose Water Quality Trading (WQT) to minimize the cost of controlling water pollution and develop it for policy recommendations in the River Alde catchment in Suffolk. We apply WQT to three scenarios named Reference Pollution Target, Livestock Target Plan and Variation of Farming. Our findings demonstrate that WQT can reduce farmers nitrogen load by 8%, 7% and 18% respectively from the baseline of 6 mg/L. The scenario simulations show a net revenue increase of 6%, 5% and 18% respectively. Our study demonstrates the effectiveness of the WQT approach in reducing water pollution, promoting sustainable agriculture and meeting water management goals.

DNA methylation-based profiling for paediatric CNS tumour diagnosis and treatment: a population-based study.

BACKGROUND: Marked variation exists in the use of genomic data in tumour diagnosis, and optimal integration with conventional diagnostic technology remains uncertain despite several studies reporting improved diagnostic accuracy, selection for targeted treatments, and stratification for trials. Our aim was to assess the added value of molecular profiling in routine clinical practice and the impact on conventional and experimental treatments. METHODS: This population-based study assessed the diagnostic and clinical use of DNA methylation-based profiling in childhood CNS tumours using two large national cohorts in the UK. In the diagnostic cohort-which included routinely diagnosed CNS tumours between Sept 1, 2016, and Sept 1, 2018-we assessed how the methylation profile altered or refined diagnosis in routine clinical practice and estimated how this would affect standard patient management. For the archival cohort of diagnostically difficult cases, we established how many cases could be solved using modern standard pathology, how many could only be solved using the methylation profile, and how many remained unsolvable. FINDINGS: Of 484 patients younger than 20 years with CNS tumours, 306 had DNA methylation arrays requested by the neuropathologist and were included in the diagnostic cohort. Molecular profiling added a unique contribution to clinical diagnosis in 107 (35%; 95% CI 30-40) of 306 cases in routine diagnostic practice-providing additional molecular subtyping data in 99 cases, amended the final diagnosis in five cases, and making potentially significant predictions in three cases. We estimated that it could change conventional management in 11 (4%; 95% CI 2-6) of 306 patients. Among 195 historically difficult-to-diagnose tumours in the archival cohort, 99 (51%) could be diagnosed using standard methods, with the addition of methylation profiling solving a further 34 (17%) cases. The remaining 62 (32%) cases were unresolved despite specialist pathology and methylation profiling. INTERPRETATION: Together, these data provide estimates of the impact that could be expected from routine implementation of genomic profiling into clinical practice, and indicate limitations where additional techniques will be required. We conclude that DNA methylation arrays are a useful diagnostic adjunct for childhood CNS tumours. FUNDING: The Brain Tumour Charity, Children with Cancer UK, Great Ormond Street Hospital Children's Charity, Olivia Hodson Cancer Fund, Cancer Research UK, and the National Institute of Health Research.