1.
Biomed Chromatogr
; 17(8): 526-9, 2003 Dec.
Artigo
em Inglês
| MEDLINE
| ID: mdl-14648610
RESUMO
Reversed-phase TLC systems using ion-pairing reagents are described for the separation without prior derivatization of the porphyrins of rat Harderian gland. Porphyrins with R(f) values greater than protoporphyrin but smaller than coproporphyrin, representing harderoporphyrin, were labile to 1 m NaOH, which converted them to protoporphyrin, inconsistent with the established literature that harderoporphyrin is a tricarboxylic acid porphyrin. It was confirmed by HPLC/electrospray ionization MS that this 'harderoporphyrin' fraction consists of the recently characterized glycoconjugate, protoporphyrin-1-O-acyl beta-xyloside, with trace amounts of protoporphyrin-1-O-acyl beta-glucoside.
Assuntos
Cromatografia em Camada Fina/métodos , Glândula de Harder/química , Porfirinas/análise , Espectrometria de Massas por Ionização por Electrospray/métodos , Ácidos Tricarboxílicos/química , Animais , Feminino , Masculino , Porfirinas/química , Ratos , Ratos Wistar
2.
Clin Chem
; 48(3): 564-6, 2002 Mar.
Artigo
em Inglês
| MEDLINE
| ID: mdl-11861450