Transgeneration effects of N-stearoylethanolamine in irradiated rats. / TRANSGENERATsIY̆NI EFEKTY N-STEAROÏLETANOLAMINU V OPROMINENYKh ShchURIV.

OBJECTIVE: to explore possible transgeneration effects in the rats offspring of the first generation of parents subject ed to the combined effects of N stearoylethanolamine (NSE) and external exposure.Materials and metods. In the first generation rats of both sexes born to parents who have experienced the combined influence of ionizing radiation at a dose of 2.0 Gy and NSE a daily dose of 50.0 mg/kg, administered before or after exposure indicators of pro and antioxidant systems (the concentration of TBA reactive products, catalase and glu tathione peroxidase activity in plasma) were defined, concentrations of sex hormones testosterone and estradiol and nitrite anion were studied. RESULTS: Irradiation of parents caused a three fold reduction of testosterone in the blood plasma of males progeny, increased activity of catalase in plasma of female offsprings, as well as significantly increased the concentration of protein in the offsprings' blood plasma of both sexes. Introduction of NSE to parents before exposure caused the acti vation of lipid peroxidation in plasma of both sexes offsprings' against the background of a trustworthy decrease in activity of antioxidant enzymes (catalase and glutathione peroxidase), however, prevented a sharp reduction of testosterone content in the blood plasma of males offsprings, conditioned by the influence of radiation on the body of their parents. NSE introduction to parents after exposure caused no significant violations of pro/ antioxidant bal ance in the body of both sexes progeny, but did not eliminate the negative impact of parental exposure to testos terone levels in male offsprings. CONCLUSION: The transgeneration impact of NSE is manifested by radio sensitizing properties in the first generation offsprings in case of application to parents before irradiation.

Long-chain N-acylethanolamines inhibit lipid peroxidation in rat liver mitochondria under acute hypoxic hypoxia.

Two long-chain N-acylethanolamines (NAEs), N-palmitoyl- (NPE) and N-stearoylethanolamine (NSE), are shown to inhibit an in vitro non-enzymatic Fe(2+)-induced free radical oxidation of lipids in the liver mitochondria of rats with hypoxic hypoxia. NSE appeared to be more effective than NPE in suppressing some kinetic parameters of the Fe(2+)-induced chemiluminescence. The inhibitory action of NAEs on non-enzymatic lipid peroxidation supports the idea that they possess membrane protective properties.