RESUMO
This study analyses the analgesic effect of a hydroalcoholic extract (HE) from Phyllanthus corcovadensis in several models of pain in mice. HE (3-60 mg kg-1, i.p.) or (100-500 mg kg-1, p.o.) caused a graded and potent analgesic effect against the abdominal constriction response caused by acetic acid and acetylcholine with an ID50 of about 3 and 100 mg kg-1, respectively. In the tail-flick model HE (up to 500 mg kg-1, p.o.) was without effect, while morphine (1-10 mg kg-1, s.c.) caused a graded increase in pain latency (ID50, 3 mg kg-1). HE (1-300 mg kg-1) given both intraperitoneally and orally caused a potent and graded inhibition of the second phase of formalin-induced persistent pain in mice with an ID50 of 1 and 80 mg kg-1, respectively. In contrast, morphine (1-5 mg kg-1, s.c.) inhibited both phases of formalin-induced pain with an ID50 of 2.5 mg kg-1. Indomethacin (1-10 mg kg-1, i.p.) only inhibited the second phase of formalin-induced pain with an ID50 of about 3 mg kg-1. The analgesic effect of indomethacin, but not that caused by morphine and HE was accompanied by a graded inhibition of formalin-induced mouse paw oedema. In addition, HE up to 1 g kg-1 failed to prevent carrageenan- and dextran-induced rat hindpaw oedema. It is concluded that HE exhibits a potent antinociceptive profile, either when given intraperitoneally or orally.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Analgésicos/farmacologia , Anti-Inflamatórios/farmacologia , Nociceptores/efeitos dos fármacos , Extratos Vegetais/farmacologia , Plantas Medicinais , Animais , Carragenina , Dextranos , Relação Dose-Resposta a Droga , Edema/induzido quimicamente , Edema/tratamento farmacológico , Etanol , Formaldeído , Masculino , Camundongos , Modelos Biológicos , Medição da Dor/efeitos dos fármacos , RatosRESUMO
The 10 patients with atopic asthma and 6 healthy subjects were selected for the study. Each person underwent prick testing to common allergens (extracts of house dust, grass, trees, animal danders, feathers and mould). In addition blood was drawn for the specific IgE antibodies to D. pteronyssinus and total serum IgE levels. Neutrophil and eosinophil chemiluminescence induced by PMA and FMLP was measured in both groups. Eosinophils obtained from patients with atopic asthma stimulated with PMA and FMLP generated significantly greater free radicals than obtained from normal subjects. There was no observed significant difference of neutrophil chemiluminescence activated by PMA and FMLP and of the percentage of hypodense eosinophils in the peripheral blood between subjects with asthma and healthy ones.