1.
Bioorg Med Chem Lett
; 10(23): 2639-42, 2000 Dec 04.
Artigo
em Inglês
| MEDLINE
| ID: mdl-11128641
RESUMO
A statistically exhaustive, 8800 compound tripeptidal amidomethylcoumarin library was synthesized as discreet compounds using solid-phase combinatorial chemistry. A subset of the compounds was purified by HPLC and tested in a high-throughput fluorometric assay against several known serine and cysteine proteases to demonstrate the utility of this library for profiling protease substrate specificity.
Assuntos
Cumarínicos/síntese química , Endopeptidases/metabolismo , Corantes Fluorescentes/síntese química , Cromatografia Líquida de Alta Pressão , Técnicas de Química Combinatória , Cumarínicos/química , Corantes Fluorescentes/química , Sondas Moleculares , Especificidade por Substrato
2.