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1.
Cancer Lett ; 292(2): 171-5, 2010 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-20036459

RESUMO

Human Papillomaviruses (HPVs) have been found in association with benign and malignant growth of epithelia. The cell cycle inhibitor p16(Ink4a) has been shown to be overexpressed in HPV-positive cervical pre-malignant and malignant lesions, probably as a result of pRB targeting by the viral E7 protein. Inverted papillomas of the urinary bladder are epithelial tumors considered to be of benign nature. In this report we analyze the expression of p16(Ink4a) and the presence of HPV sequences in inverted papillomas and in non-tumoral bladder controls. Our results show no association of HPV infection and inverted papillomas. Further, no correlation between p16 overexpression and HPV positivity was found. We conclude that HPV does not play an indispensable role in the development of urinary bladder inverted papillomas and that overexpression of p16(Ink4a) does not correlate with HPV infection in these tumors.


Assuntos
Alphapapillomavirus/isolamento & purificação , Inibidor p16 de Quinase Dependente de Ciclina/metabolismo , Papiloma/metabolismo , Papiloma/virologia , Neoplasias da Bexiga Urinária/metabolismo , Neoplasias da Bexiga Urinária/virologia , Alphapapillomavirus/genética , Genótipo , Humanos , Imuno-Histoquímica , Reação em Cadeia da Polimerase , Neoplasias da Bexiga Urinária/patologia
2.
Mod Pathol ; 19(5): 630-3, 2006 May.
Artigo em Inglês | MEDLINE | ID: mdl-16528369

RESUMO

uPM3 is the first urine-based genetic test that is highly specific for detecting prostate cancer. The histopathologic characteristics of uPM3-detected cancer in radical prostatectomy specimens have not been previously described. We evaluated a consecutive series of radical prostatectomies to determine the extent, zonal distribution and other features of prostate cancer following uPM3 detection. A total of 24 whole-mounted, totally embedded radical prostatectomy specimens were evaluated. All patients had clinically localized cancer and none received preoperative therapy. Zonal location of cancer, distance to the urethra, cancer volume, Gleason grade and multicentricity were recorded; volume of cancer was measured using the grid-counting method. Patients ranged in age from 43 to 75 years (mean 65 years). In 21/24 cases, cancer involved the transition and peripheral zones and was multicentric (87%). Mean cancer volume was 3.96 cm3 (range 0.08-16.86 cm3). Mean distance to the urethra was 0.50 cm for the closest cancer and 0.61 cm for the most distant cancer. Mean Gleason score was 7 (range 5-9); pathologic stage was pT2 for 17 cases and pT3 for seven cases. The uPM3 is an independent and specific biomarker that detects prostate cancers of similar volume, location, extent and grade as other tests for early detection. uPM3 does not preferentially identify large or aggressive prostate cancers.


Assuntos
Antígenos de Neoplasias/genética , Neoplasias da Próstata/diagnóstico , Adulto , Idoso , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Próstata/metabolismo , Próstata/patologia , Próstata/cirurgia , Prostatectomia/métodos , Neoplasias da Próstata/genética , Neoplasias da Próstata/urina , RNA Mensageiro/genética , RNA Mensageiro/urina , RNA Neoplásico/genética , RNA Neoplásico/urina , Sensibilidade e Especificidade
3.
Hum Pathol ; 36(5): 536-45, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15948121

RESUMO

We applied an antiserum (SA226P) specifically recognizing the phosphorylated form of connexin43 (P-Cx43) to human breast samples including normal breast samples, with fibrocystic disease (FCD), fibroadenomas (FA), in situ and infiltrating carcinomas of all major types, and miscellaneous extramammary tumors. The findings were compared with those obtained with commercial antisera recognizing all Cx43 forms (pan-Cx43). A subset of samples was stained for Her2-neu and p44/42 to mitogen-activated protein kinase. Paraffin step sections were used. Immunoblots were performed on frozen samples of a representative subset of cases. In the normal breast, FCD, and FA, SA226P stained strongly and extensively most myoepithelial cells (MECs); luminal cells remained unstained. In proliferative FCD and some cellular FA, SA226P stained MEC and the capillary endothelium (CE). In ductal and lobular in situ carcinomas, SA226P reacted strongly and diffusely with the remaining MEC, the CE, and the transformed luminal cells. SA226P stained all infiltrating carcinomas except the tubular variant. In all breast carcinomas, the CE within and adjacent to tumors and some myofibroblasts stained with SA226P. By contrast, pan-Cx43 stained weakly and sporadically the MEC and rare samples of invasive carcinomas. Notably, Mab p44/42 reacted in parallel with the samples stained with SA226P, whereas reactions with Her2 were negative. Immunoblot findings paralleled those obtained immunohistochemically. We conclude that P-Cx43, restricted to MEC in the normal breast, is up-regulated in the same cells in hyperplasias and dysplasias and FA and is strongly up-regulated in invasive carcinomas. Notably, in some proliferative FCD and in most in situ and infiltrating carcinomas, P-Cx43 is strongly expressed in CE within and adjacent to the lesions but not away from them. These findings were paralleled by the strong nuclear reactions noted with Mab p44/42. These phenomena, although not exclusive to malignancy, are particularly conspicuous in breast carcinomas and seemingly reflect active proliferation associated with abnormal gap junctional intercellular communication.


Assuntos
Doenças Mamárias/metabolismo , Neoplasias da Mama/metabolismo , Mama/metabolismo , Conexina 43/biossíntese , Hiperplasia/metabolismo , Mama/irrigação sanguínea , Mama/patologia , Doenças Mamárias/patologia , Neoplasias da Mama/irrigação sanguínea , Neoplasias da Mama/patologia , Capilares/metabolismo , Carcinoma Ductal de Mama/irrigação sanguínea , Carcinoma Ductal de Mama/metabolismo , Carcinoma Ductal de Mama/patologia , Carcinoma Intraductal não Infiltrante/irrigação sanguínea , Carcinoma Intraductal não Infiltrante/metabolismo , Carcinoma Intraductal não Infiltrante/patologia , Conexina 43/química , Endotélio Vascular/metabolismo , Células Epiteliais/metabolismo , Feminino , Fibroadenoma/irrigação sanguínea , Fibroadenoma/metabolismo , Fibroadenoma/patologia , Doença da Mama Fibrocística/irrigação sanguínea , Doença da Mama Fibrocística/metabolismo , Doença da Mama Fibrocística/patologia , Humanos , Hiperplasia/patologia , Immunoblotting , Imuno-Histoquímica , Proteína Quinase 1 Ativada por Mitógeno/biossíntese , Proteína Quinase 3 Ativada por Mitógeno/biossíntese , Fosforilação , Receptor ErbB-2/biossíntese , Regulação para Cima
4.
Hum Pathol ; 36(4): 325-9, 2005 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15891991

RESUMO

Epstein-Barr virus (EBV) has been linked etiologically to infectious mononucleosis, some non-Hodgkin as well as Hodgkin lymphomas, and lymphoepithelioma-like carcinomas. Moreover, various EBV antigens have been identified by a variety of techniques in a number of visceral carcinomas including breast, prostate, colon and lung primaries. We have now demonstrated by immunohistochemistry the presence of EBV nuclear antigen-1 (EBNA-1) in 4 of 15 cases of conjuntival squamous carcinomas and related dysplasias. At present, there is no significant evidence linking etiologically EVB to this type of tumor and dysplasia. However, our findings merit further investigation given the growing evidence that EBV may enhance proliferation and aggressiveness of tumor systems as well as the immortalization of non-neoplastic cells.


Assuntos
Carcinoma in Situ/virologia , Carcinoma de Células Escamosas/virologia , Neoplasias da Túnica Conjuntiva/virologia , Antígenos Nucleares do Vírus Epstein-Barr/análise , Infecções Tumorais por Vírus/virologia , Idoso , Carcinoma in Situ/patologia , Carcinoma de Células Escamosas/patologia , Neoplasias da Túnica Conjuntiva/patologia , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Lesões Pré-Cancerosas/patologia , Lesões Pré-Cancerosas/virologia , Infecções Tumorais por Vírus/patologia
5.
World J Gastroenterol ; 11(1): 94-8, 2005 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-15609404

RESUMO

AIM: To investigate the clinicopathological factors underlying the ethnic differences of Helicobacter pylori gastritis and cancer. METHODS: We analyzed clinicopathological parameters of gastric biopsies having H pylori infection that were randomly selected from different ethnic populations including 147 Americans, 149 Japanese, and 181 Koreans. RESULTS: Males were predominant in Japanese and Korean populations (77.9 and 67.4% respectively) in comparison with Americans (48.3%) (P<0.001). H pylori gastritis in Koreans and Japanese was characterized by the predominant antral involvement. In the antrum, neutrophilic infiltration into the proliferative zone of pit, i.e. acute foveolitis, was more frequent in Koreans (82%) than in Japanese (71%) (P<0.05) and Americans (61%) (P<0.001). Interstitial neutrophilic infiltration, intestinal metaplasia and atrophy were also frequent in Koreans and Japanese. In the body, the prevalence of acute foveolitis was not significantly different among the populations while chronic interstitial inflammation and lymphoid follicles were more pronounced in the body of Americans than in the body of others (P<0.01). CONCLUSION: The male-, and antrum-predominant H pylori gastritis in Koreans and Japanese is compatible with the pattern of sex and topographical distribution of gastric cancer incidence. Our data suggest that persistent acute foveolitis at the proliferative zone is a crucial step in the gastric carcinogenesis.


Assuntos
Gastrite/etnologia , Infecções por Helicobacter/etnologia , Helicobacter pylori , Antro Pilórico/microbiologia , Antro Pilórico/patologia , Adulto , Atrofia , Biópsia , Doença Crônica , Células Epiteliais/imunologia , Células Epiteliais/microbiologia , Células Epiteliais/patologia , Feminino , Gastrite/imunologia , Gastrite/patologia , Infecções por Helicobacter/imunologia , Infecções por Helicobacter/patologia , Humanos , Intestinos/patologia , Japão/epidemiologia , Coreia (Geográfico)/epidemiologia , Tecido Linfoide/patologia , Masculino , Metaplasia , Pessoa de Meia-Idade , Neutrófilos/patologia , Antro Pilórico/imunologia , Distribuição por Sexo , Estados Unidos/epidemiologia
6.
Diagn Cytopathol ; 30(3): 201-7, 2004 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-14986303

RESUMO

We present cytologic data from multiple samples from two cases of inverted sinonasal papilloma (ISP). These samples displayed the entire spectrum of squamous cell changes, including benign squamous papilloma, variable degrees of dysplasia, and invasive squamous cell carcinoma. In all instances, the cytologic impression coincided with the final diagnosis based on frozen and/or permanent histologic sections from the same samples. We suggest that cytologic examination be viewed as a useful initial approach in the diagnosis of ISP, and in the differential diagnosis of other tumors that occur in the same sites.


Assuntos
Carcinoma de Células Escamosas/diagnóstico , Neoplasias Primárias Múltiplas/diagnóstico , Papiloma Invertido/patologia , Neoplasias dos Seios Paranasais/patologia , Adulto , Núcleo Celular/patologia , Terapia Combinada , Citoplasma/patologia , Diagnóstico Diferencial , Humanos , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia , Papiloma Invertido/terapia , Neoplasias dos Seios Paranasais/terapia , Resultado do Tratamento
7.
Appl Immunohistochem Mol Morphol ; 11(2): 120-4, 2003 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12777994

RESUMO

HER-2/Neu overexpression is seen in 20% to 30% of invasive breast carcinomas and has been reported in as many as 80% of high-grade infiltrating carcinomas. Earlier studies have suggested that 100% of the tumor cells in mammary Paget disease show overexpression of HER-2 protein. We undertook this study to assess HER-2 status of mammary Paget disease and of the underlying breast carcinoma, when present, by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). Formalin-fixed, paraffin-embedded tissue from 20 cases of mammary Paget disease were analyzed for HER-2 status by IHC and FISH. IHC for estrogen receptor (ER) was also performed. The patients ranged in age from 34 to 88 years, with a mean age of 62 years. Eighty percent of the cases showed strong overexpression (3+) of HER-2 protein by IHC, and all of these cases showed more than 5-fold amplification of the HER-2 gene by FISH. The remaining 4 cases, which were negative for HER-2/Neu by IHC, showed no amplification by FISH. All of the latter cases expressed ER, whereas no case that overexpressed HER-2 expressed ER. Sixteen cases had an underlying tumor, which was in situ in 6 cases. The underlying tumors were identical to the Paget disease with respect to their HER-2/Neu overexpression by both IHC and FISH. HER-2 overexpression was identified in 80% of our cases of Paget disease. There was 100% concordance between HER-2 protein overexpression by immunohistochemistry and gene amplification in both the Paget and the underlying tumor. Moreover, all of the cases negative for HER-2 overexpression expressed ER, whereas those positive for HER-2 did not.


Assuntos
Doença de Paget Mamária/química , Receptor ErbB-2/análise , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Amplificação de Genes , Humanos , Imuno-Histoquímica , Hibridização in Situ Fluorescente , Pessoa de Meia-Idade , Proteínas de Neoplasias/análise , Doença de Paget Mamária/patologia , Receptores de Estrogênio/análise
8.
Am J Pathol ; 162(4): 1203-11, 2003 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-12651612

RESUMO

Cancers may develop in the background of genomic instability with accumulated mutations. Helicobacter pylori gastritis is characterized by acute foveolitis of the proliferative zone, which is found in any stage of the gastritis as long as the infection persists. Because acute foveolitis targets specifically the proliferative zone of pits, the proliferating epithelial cells are under severe and persistent mutagenic pressure. In H. pylori gastritis, a characteristic morphological change of epithelial cells, the malgun (clear) cell change is frequently present in association with acute foveolitis. Malgun cells have enlarged euchromatic nuclei and abundant cytoplasm. The expression of proliferating cell nuclear antigen and cytokeratin 8 are typically up-regulated in them indicating that they are mitotically and metabolically active. Here, we report evidence for DNA damage and repair in malgun cells. Significant double-strand DNA breaks were shown by the consistent terminal dUTP nick-end labeling in the nuclei of malgun cells. Proteins related to DNA damage and repair, such as Ku, poly(ADP-ribosyl) polymerase, OGG1, and MSH2 were selectively up-regulated in malgun cells. Inducible nitric oxide synthase was also up-regulated. There were occasional bcl2- and p53-expressing cells suggesting that further steps of carcinogenesis took place at the single cell level. Our results suggest that the malgun cell change represents a characteristic morphological sign of cellular genomic damage and repair, and may be implicated in an early stage of carcinogenesis. It is suggested that acute foveolitis of the proliferative zone is a major pathogenetic step of gastric carcinogenesis in H. pylori gastritis.


Assuntos
Dano ao DNA/genética , Reparo do DNA/genética , Mucosa Gástrica/patologia , Gastrite/microbiologia , Infecções por Helicobacter/genética , Infecções por Helicobacter/patologia , Helicobacter pylori , Adulto , Idoso , Biópsia , Divisão Celular , Gastrite/genética , Gastrite/patologia , Humanos , Imuno-Histoquímica , Pessoa de Meia-Idade
9.
Urology ; 61(1): 248-52, 2003 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-12559317

RESUMO

OBJECTIVES: To review the expression of A-80 in prostate cancer and prostatic intraepithelial neoplasia and compare it with that of normal and hyperplastic prostatic tissue. The ability to recognize cancer after androgen deprivation therapy and residual and/or recurrent cancer after radiotherapy using A-80 staining was also examined. A-80 is a glycoprotein linked to exocrine differentiation that shows little or no expression in normal exocrine cells, but is selectively overexpressed in dysplasias and adenocarcinomas. METHODS: We studied 277 prostate samples with a monoclonal antibody (MAb) to A-80. We applied this MAb to paraffin sections of specimens of fetal (n = 12), benign prostatic hyperplasia (n = 26, from transurethral prostate resection specimens), atypical adenomatous hyperplasia (n = 11), prostate cancer (n = 103, from radical prostatectomy specimens), and autopsy (n = 7) tissue. In addition, 54 prostatectomy specimens after androgen-deprivation therapy and 64 specimens after radiotherapy were similarly studied. RESULTS: MAb A-80 stained the epithelial component of all 12 prostate specimens from fetal tissue; no staining was seen in normal adult prostatic tissue (0 of 7). In benign prostatic hyperplasia, sporadic cells reacted in 13% of cases (4 of 30); the atypical adenomatous hyperplasia samples were all negative (0 of 11). In patients with prostate cancer, more than 99% (102 of 103) stained positive, regardless of the grade or stage of cancer. Low and high-grade prostatic intraepithelial neoplasia reacted in 73% (38 of 52) and 92% (77 of 84) of cases, respectively. All 64 (100%) salvage prostatectomy samples after external beam radiotherapy stained positive for A-80. Carcinoma subsequent to neoadjuvant hormonal therapy stained positive in 98% (53 of 54) of cases. CONCLUSIONS: A-80 is useful in differentiating benign prostatic hyperplasia and atypical adenomatous hyperplasia from prostate cancer. Also, the strong A-80 reactions in most high-grade prostatic intraepithelial neoplasia provide strong molecular support to the precancerous nature of the lesion. In particular, A-80 staining of biopsies may be useful in detecting residual and/or recurrent prostate carcinoma after radiation or hormonal therapy.


Assuntos
Adenocarcinoma/química , Adenocarcinoma/genética , Glicoproteínas/análise , Próstata/química , Hiperplasia Prostática/diagnóstico , Neoplasia Prostática Intraepitelial/química , Neoplasias da Próstata/química , Neoplasias da Próstata/genética , Adenocarcinoma/diagnóstico , Antagonistas de Androgênios/uso terapêutico , Anticorpos Monoclonais , Diagnóstico Diferencial , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , Próstata/cirurgia , Antígeno Prostático Específico/sangue , Prostatectomia , Hiperplasia Prostática/genética , Hiperplasia Prostática/metabolismo , Neoplasia Prostática Intraepitelial/diagnóstico , Neoplasia Prostática Intraepitelial/genética , Neoplasias da Próstata/diagnóstico
11.
Breast J ; 8(6): 349-55, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12390357

RESUMO

It is important to identify T1-substage breast carcinomas (BCs) which are inherently aggressive, so that these can be managed more assertively. The purpose of this study was to distinguish those T1 BCs with the potential to metastasize to axillary lymph nodes from those lacking that ability by multiparametric analysis of several clinicopathologic features. The authors studied 197 patients with invasive BC who had undergone modified radical mastectomy; 161 tumors were ductal and 26 were lobular BCs. The study group was stratified by age into two groups:

Assuntos
Biomarcadores Tumorais/metabolismo , Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Linfonodos/patologia , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Axila , Feminino , Humanos , Imuno-Histoquímica , Antígeno Ki-67/metabolismo , Modelos Logísticos , Metástase Linfática/patologia , Metástase Linfática/prevenção & controle , Pessoa de Meia-Idade , Análise Multivariada , Estadiamento de Neoplasias , Razão de Chances , Valor Preditivo dos Testes , Receptores de Estrogênio/metabolismo , Receptores de Progesterona/metabolismo , Proteína Supressora de Tumor p53/metabolismo
12.
Surg Clin North Am ; 82(3): 525-40, vi, 2002 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12371583

RESUMO

This article is an overview of the classification of pulmonary neuroendocrine neoplasms, their presentation, their pathologic appearance, and their clinical management. In addition, the original classification, based on histologic features, is reassessed in the light of newer areas in study, including neurosecretory products, neuroendocrine markers, ultrastructural studies, ploidy analysis, cell adhesion markers, apoptosis, oncogene mutation analysis, and genetic alterations. The histologic classification proposed in 1983 remains the single most valuable factor in establishing the diagnosis and, together with the TNM status, the prognosis of this group of interesting neoplasms.


Assuntos
Neoplasias Brônquicas/classificação , Neoplasias Pulmonares/classificação , Tumores Neuroendócrinos/classificação , Neoplasias Brônquicas/diagnóstico por imagem , Neoplasias Brônquicas/patologia , Humanos , Neoplasias Pulmonares/diagnóstico por imagem , Neoplasias Pulmonares/patologia , Tumores Neuroendócrinos/diagnóstico por imagem , Tumores Neuroendócrinos/patologia , Radiografia , Fatores de Tempo
14.
Cancer Res ; 62(17): 4876-8, 2002 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-12208733

RESUMO

EBV is an etiological agent in infectious mononucleosis, is implicated in some malignant lymphomas and lymphoepithelioma-like carcinomas, and has been sporadically reported in carcinomas of the breast, lung, and other sites. We studied immunohistochemically benign and malignant tumors of the breast, lung, colon, and prostate and found EBV in some carcinomas of those sites. Also, EBV reactions were noted in hyperplasias and dysplasias, e.g., breast carcinomas in situ and prostatic intraepithelial neoplasia. Benign tumor counterparts were negative. PCR analysis of selected cases confirmed the presence of EBV. Our results suggest that EBV is not restricted to lymphoepithelioma-like carcinomas but may play an oncogenic role in frequent epithelial cancers and possibly also in hyperplasias and certain dysplasias preceding carcinomas.


Assuntos
Carcinoma/virologia , Infecções por Vírus Epstein-Barr/complicações , Herpesvirus Humano 4 , Neoplasias/virologia , Neoplasias da Mama/virologia , Neoplasias do Colo/virologia , Infecções por Vírus Epstein-Barr/virologia , Antígenos Nucleares do Vírus Epstein-Barr/metabolismo , Feminino , Herpesvirus Humano 4/genética , Herpesvirus Humano 4/metabolismo , Humanos , Imuno-Histoquímica , Neoplasias Pulmonares/virologia , Masculino , Reação em Cadeia da Polimerase , Neoplasias da Próstata/virologia , RNA Viral/genética
15.
Diagn Cytopathol ; 26(6): 384-6, 2002 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12112829

RESUMO

A 4(1/2)-yr-old female presented with right-sided pleural effusion and a retroperitoneal mass. Cytologic analysis of the pleural fluid yielded malignant small round blue cells, which were noncohesive, 3-4 times the size of lymphocytes. The malignant cells had hyperchromatic, pleomorphic nuclei with moderate amounts of vacuolated cytoplasm. A few fiber-shaped cells were also seen. Immunostains for desmin, muscle-specific actin were positive; ultrastructural findings of thick and thin actin-myosin filaments confirmed the diagnosis of embryonal rhabdomyosarcoma. This case illustrates the importance of performing appropriate immunohistochemical stains and ultrastructural studies on cytological material to arrive at a definitive diagnosis.


Assuntos
Neoplasias Abdominais/diagnóstico , Rabdomiossarcoma/diagnóstico , Neoplasias Abdominais/diagnóstico por imagem , Neoplasias Abdominais/patologia , Pré-Escolar , Diagnóstico Diferencial , Feminino , Humanos , Rabdomiossarcoma/diagnóstico por imagem , Rabdomiossarcoma/patologia , Tomografia Computadorizada por Raios X
16.
Hum Pathol ; 33(5): 536-44, 2002 May.
Artigo em Inglês | MEDLINE | ID: mdl-12094380

RESUMO

Nuclear pore complexes (NPCs) are elaborate macromolecular structures that regulate the bidirectional nucleocytoplasmic traffic system. In vertebrate cells, NPCs include a family of 50 to 100 proteins termed nucleoporins (Nups). The 88-kD Nup has been found to be linked in a dynamic subcomplex with the oncogenic CAN/Nup214. Applying a polyclonal antiserum to Nup88 on paraffin sections, we found that it immunoreacts with numerous malignant neoplasms. All carcinomas reacted irrespective of site, type, or degree of differentiation; often, high-grade carcinomas stained more strongly and extensively. Some sarcomas (e.g., fibrosarcomas, leiomyosarcomas, liposarcomas, and rhabdomyosarcomas) reacted intensely; melanomas, gliomas, mesotheliomas, and malignant lymphomas also stained. In situ carcinomas of the colon, stomach, breast, and prostate stained convincingly, as did in situ melanomas; some samples of fetal tissues also reacted. Cytologic smears of some of the aforementioned tumors also stained. In selected samples, enhanced immunostaining of tissue sections and cytologic smears correlated strongly and consistently with immunoblot data. Immunoblots of the same tumors with antibodies to 2 other Nups (Nup214 and Nup153) showed no comparable enhancement. Therefore, it seems that in some malignant tumors, Nup88 overexpression is not parallelled by an overexpression of other Nups. Benign tumors, hyperplasias, and normal tissues showed weak and sporadic staining or absence of staining; immunoblots of the same samples yielded weak signals. Occasional highly proliferative hyperplastic-reactive processes showed focal staining. Thus, our correlative histologic, cytologic, and molecular data indicate that Nup88 may be viewed as a potentially useful, broadly based histodiagnostic and molecular marker of many malignancies and premalignant dysplasias, and further suggest that in some malignant tumors, Nup88 may be selectively overexpressed as compared with other Nups. Thus, we propose that Nup88 be designated as karyoporin.


Assuntos
Neoplasias/metabolismo , Complexo de Proteínas Formadoras de Poros Nucleares/metabolismo , Lesões Pré-Cancerosas/metabolismo , Biomarcadores Tumorais/metabolismo , Citodiagnóstico , Feminino , Humanos , Immunoblotting , Imuno-Histoquímica , Masculino , Neoplasias/patologia , Lesões Pré-Cancerosas/patologia
17.
Breast J ; 8(2): 101-7, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-11896756

RESUMO

Tumor expression of the proliferation marker (MIB-1) and the cell cycle-related protein (p27) may predict the biologic behavior of various human tumors. The purpose of this study was to evaluate the role of p27 and MIB-1 expression in predicting lymph node metastasis in male breast carcinomas (MBCs). We studied 67 patients with invasive MBC who had undergone modified radical mastectomy. Pathologic lymph node status was correlated with the p27 protein and the MIB-1 proliferation index. These factors were studied immunohistologically by standard methods. Men in this study ranged from 36 to 92 years of age (mean, 63 years); 43 (64%) were T1 lesions, and 24 (36%) were T2 lesions. Twenty-nine patients (43%) had positive nodes. p27 was expressed in 43 tumors (64%) and MIB-1 in 13 tumors (19.4%). Tumors with positive p27 showed positive lymph nodes in 10 cases (23%). In contrast, p27-negative tumors had positive lymph nodes in 18 cases (75%). Tumors positive for MIB-1 show positive lymph nodes in 11 cases (85%). However, when MIB-1 was negative, only 16 patients (30%) had positive lymph nodes. Multivariate logistic regression analysis confirmed the utility of MIB-1 overexpression in predicting lymph node metastasis ( p < 0.0006). Also, decreased p27 protein expression strongly correlates with lymph node metastasis ( p < or = 0.0001). Furthermore, when p27 was negative and MIB-1 was positive, 100% of the patients had positive lymph nodes. In contrast, when p27 was positive and MIB-1 was negative, only 12% of patients had positive lymph nodes. The reduced expression of the p27 protein and the overexpression of the MIB-1 proliferation index in this study show a significant correlation in predicting lymph nodes metastasis in MBCs.


Assuntos
Biomarcadores Tumorais/metabolismo , Neoplasias da Mama Masculina/metabolismo , Neoplasias da Mama Masculina/patologia , Proteínas Nucleares/metabolismo , Antígeno Nuclear de Célula em Proliferação/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Antígenos Nucleares , Ciclo Celular , Distribuição de Qui-Quadrado , Regulação Neoplásica da Expressão Gênica , Humanos , Imuno-Histoquímica , Antígeno Ki-67 , Metástase Linfática/patologia , Metástase Linfática/prevenção & controle , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Valor Preditivo dos Testes
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