Design, Fabrication, and Evaluation of 3D Biopotential Electrodes and Intelligent Garment System for Sports Monitoring.

This study presents the development and evaluation of an innovative intelligent garment system, incorporating 3D knitted silver biopotential electrodes, designed for long-term sports monitoring. By integrating advanced textile engineering with wearable monitoring technologies, we introduce a novel approach to real-time physiological signal acquisition, focusing on enhancing athletic performance analysis and fatigue detection. Utilizing low-resistance silver fibers, our electrodes demonstrate significantly reduced skin-to-electrode impedance, facilitating improved signal quality and reliability, especially during physical activities. The garment system, embedded with these electrodes, offers a non-invasive, comfortable solution for continuous ECG and EMG monitoring, addressing the limitations of traditional Ag/AgCl electrodes, such as skin irritation and signal degradation over time. Through various experimentation, including impedance measurements and biosignal acquisition during cycling activities, we validate the system's effectiveness in capturing high-quality physiological data. Our findings illustrate the electrodes' superior performance in both dry and wet conditions. This study not only advances the field of intelligent garments and biopotential monitoring, but also provides valuable insights for the application of intelligent sports wearables in the future.

Day-to-Day Variability in Measurements of Respiration Using Bioimpedance from a Non-Standard Location.

Non-invasive monitoring of pulmonary health may be useful for tracking several conditions such as COVID-19 recovery and the progression of pulmonary edema. Some proposed methods use impedance-based technologies to non-invasively measure the thorax impedance as a function of respiration but face challenges that limit the feasibility, accuracy, and practicality of tracking daily changes. In our prior work, we demonstrated a novel approach to monitor respiration by measuring changes in impedance from the back of the thigh. We reported the concept of using thigh-thigh bioimpedance measurements for measuring the respiration rate and demonstrated a linear relationship between the thigh-thigh bioimpedance and lung tidal volume. Here, we investigate the variability in thigh-thigh impedance measurements to further understand the feasibility of the technique for detecting a change in the respiratory status due to disease onset or recovery if used for long-term in-home monitoring. Multiple within-session and day-to-day impedance measurements were collected at 80 kHz using dry electrodes (thigh) and wet electrodes (thorax) across the five healthy subjects, along with simultaneous gold standard spirometer measurements for three consecutive days. The peak-peak bioimpedance measurements were found to be highly correlated (0.94 ± 0.03 for dry electrodes across thigh; 0.92 ± 0.07 for wet electrodes across thorax) with the peak-peak spirometer tidal volume. The data across five subjects indicate that the day-to-day variability in the relationship between impedance and volume for thigh-thigh measurements is smaller (average of 14%) than for the thorax (40%). However, it is affected by food and water and might limit the accuracy of the respiratory tidal volume.

Dependence of Skin-Electrode Contact Impedance on Material and Skin Hydration.

Dry electrodes offer an accessible continuous acquisition of biopotential signals as part of current in-home monitoring systems but often face challenges of high-contact impedance that results in poor signal quality. The performance of dry electrodes could be affected by electrode material and skin hydration. Herein, we investigate these dependencies using a circuit skin-electrode interface model, varying material and hydration in controlled benchtop experiments on a biomimetic skin phantom simulating dry and hydrated skin. Results of the model demonstrate the contribution of the individual components in the circuit to total impedance and assist in understanding the role of electrode material in the mechanistic principle of dry electrodes. Validation was performed by conducting in vivo skin-electrode contact impedance measurements across ten normative human subjects. Further, the impact of the electrode on biopotential signal quality was evaluated by demonstrating an ability to capture clinically relevant electrocardiogram signals by using dry electrodes integrated into a toilet seat cardiovascular monitoring system. Titanium electrodes resulted in better signal quality than stainless steel electrodes. Results suggest that relative permittivity of native oxide of electrode material come into contact with the skin contributes to the interface impedance, and can lead to enhancement in the capacitive coupling of biopotential signals, especially in dry skin individuals.

A biomimetic skin phantom for characterizing wearable electrodes in the low-frequency regime.

Advances in the integration of wearable devices in our daily life have led to the development of new electrode designs for biopotential monitoring. Historically, the development and testing of wearable electrodes for the acquisition of biopotential signals has been empirical, relying on experiments on human volunteers. However, the lack of explicit control on human variables, the intra-, and inter-subject variability complicates the understanding of the performance of these wearable electrodes. Herein, phantom mimicking the electrical properties of the skin in the low-frequency range (1 Hz-1000 Hz), which has the potential to be used as a platform for controlled benchtop experiments for testing electrode functionality, is demonstrated. The fabricated phantom comprises two layers representing the deeper tissues and stratum corneum. The lower layer of the phantom mimicking deeper tissues was realized using polyvinyl alcohol cryogel (PVA-c) prepared with 0.9% W/W saline solution by a freeze-thaw technique. The properties of the upper layer representing the stratum corneum were simulated using a 100µm thick layer fabricated by spin-coating a mixture of polydimethylsiloxane (PDMS), 2.5% W/W carbon black (CB) for conductance, and 40% W/W barium titanate (BaTiO3) as a dielectric. The hydration of the stratum corneum was modeled in a controlled way by varying porosity of the phantom's upper layer. Impedance spectroscopy measurements were carried out to investigate the electrical performance of the fabricated phantom and validated against the impedance response obtained across a physiological skin impedance range of five human subjects. The results indicated that the Bode plot depicting the impedance response obtained on the phantom was found to lie in the human skin range. Moreover, it was observed that the change of porosity provides control over the hydration and the phantom can be tuned as per the skin ranges among different individuals. Also, the phantom was able to mimic the impact of dry and hydrated skin on a simulated ECG signal in the time domain. The developed skin phantom is affordable, fairly easy to manufacture, stable over time, and can be used as a platform for benchtop testing of new electrode designs.

A Wirelessly Controlled Scalable 3D-Printed Microsystem for Drug Delivery.

Here we present a 3D-printed, wirelessly controlled microsystem for drug delivery, comprising a refillable microreservoir and a phase-change peristaltic micropump. The micropump structure was inkjet-printed on the back of a printed circuit board around a catheter microtubing. The enclosure of the microsystem was fabricated using stereolithography 3D printing, with an embedded microreservoir structure and integrated micropump. In one configuration, the microsystem was optimized for murine inner ear drug delivery with an overall size of 19 × 13 × 3 mm3. Benchtop results confirmed the performance of the device for reliable drug delivery. The suitability of the device for long-term subcutaneous implantation was confirmed with favorable results of implantation of a microsystem in a mouse for six months. The drug delivery was evaluated in vivo by implanting four different microsystems in four mice, while the outlet microtubing was implanted into the round window membrane niche for infusion of a known ototoxic compound (sodium salicylate) at 50 nL/min for 20 min. Real-time shifts in distortion product otoacoustic emission thresholds and amplitudes were measured during the infusion, demonstrating similar results with syringe pump infusion. Although demonstrated for one application, this low-cost design and fabrication methodology is scalable for use in larger animals and humans for different clinical applications/delivery sites.